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公开(公告)号：US5633441A
公开(公告)日：1997-05-27
申请号：US361467
申请日：1994-12-22
申请人： Willy De Greef , John Van Emmelo , Dulce E. De Oliveira , Maria-Helena De Souza , Marc Van Montagu
发明人： Willy De Greef , John Van Emmelo , Dulce E. De Oliveira , Maria-Helena De Souza , Marc Van Montagu
IPC分类号： C07K14/415 , C12N9/10 , C12N9/22 , C12N9/50 , C12N15/55 , C12N15/62 , C12N15/82 , A01H5/00 , C12N15/11
CPC分类号： C12N9/1007 , C07K14/415 , C12N15/62 , C12N15/829 , C12N9/22 , C12N9/50 , C07K2319/00 , C07K2319/02 , Y10S47/01
摘要： The invention concerns female-sterile plants that comprise a foreign DNA incorporated in the nuclear genome of their cells. This foreign DNA first comprises a female-sterility DNA encoding a protein or polypeptide such as barnase which, when produced in the cells of the plant, kills or significantly disturbs the metabolism, functioning or development of the cells. The foreign DNA also comprises a first promoter which directs expression of the female-sterility DNA selectively in style cells, stigma cells or style-and stigma cells of the female reproductive organs of the plants. The first promoter does not direct detectable expression of the female sterility DNA in the ovule or in other parts of the plant so that the plant remains male-fertile. The female-sterility DNA is in the same transcriptional unit as and under the control of the first promoter.
摘要翻译： 本发明涉及包含掺入其细胞的核基因组中的外来DNA的雌性不育植物。 该外源DNA首先包含编码蛋白质或多肽（如芽孢杆菌RNA酶）的雌性不育DNA，当在植物细胞中产生时，它们杀死或显着地干扰细胞的代谢，功能或发育。 外源DNA还包含第一启动子，其指导雌性不育DNA在植物的雌性生殖器官的样式细胞，柱头细胞或样式和柱头细胞中的表达。 第一个启动子不能引导雌性不育DNA在胚珠或植物的其他部分中的可检测的表达，使得植物保持雄性可育。 雌性不育DNA与第一启动子处于相同的转录单位和控制下。
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公开(公告)号：US5767374A
公开(公告)日：1998-06-16
申请号：US484332
申请日：1995-06-07
申请人： Willy De Greef , John Van Emmelo , Dulce Eleonora De Oliveira , Maria-Helena De Souza , Marc Van Montagu
发明人： Willy De Greef , John Van Emmelo , Dulce Eleonora De Oliveira , Maria-Helena De Souza , Marc Van Montagu
IPC分类号： C07K14/415 , C12N9/10 , C12N9/22 , C12N9/50 , C12N15/55 , C12N15/62 , C12N15/82 , C12N5/04 , C12N15/56
CPC分类号： C12N9/1007 , C07K14/415 , C12N15/62 , C12N15/829 , C12N9/22 , C12N9/50 , C07K2319/00 , C07K2319/02 , Y10S47/01
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning and/or development of cells of the flowers, particularly one or more of their female organs, or the seeds or the embryos of the plant. The foreign DNA sequence also optionally encodes a marker.
摘要翻译： 一种植物，其核基因组用编码选择性地破坏花的细胞，特别是其一个或多个雌性器官或种子或胚胎的细胞的代谢，功能和/或发育的外源DNA序列转化 的植物。 外来DNA序列还任选地编码标记物。
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公开(公告)号：US06344598B1
公开(公告)日：2002-02-05
申请号：US08485511
申请日：1995-06-07
IPC分类号： C12N1529
CPC分类号： C12Y302/01031 , C07K14/415 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12Y301/27003
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a protein or polypeptide which disrupts the metabolism, functioning and/or development of stamen cells of the plant, said foreign DNA under the control of a stamen-specific promoter. The foreign DNA sequence also optionally encodes a marker.
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公开(公告)号：US06316699B1
公开(公告)日：2001-11-13
申请号：US08485793
申请日：1995-06-07
IPC分类号： A01H102
CPC分类号： C12Y302/01031 , C07K14/415 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12Y301/27003
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning and/or development of stamen cells of the plant. The foreign DNA sequence also optionally encodes a marker.
摘要翻译： 一种植物，其核基因组用编码选择性地破坏植物雄蕊细胞的代谢，功能和/或发育的产物的外源DNA序列转化。 外来DNA序列还任选地编码标记物。
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公开(公告)号：US5792929A
公开(公告)日：1998-08-11
申请号：US466125
申请日：1995-06-06
申请人： Celestina Mariani , Jan Leemans , Willy De Greef
发明人： Celestina Mariani , Jan Leemans , Willy De Greef
IPC分类号： C07K14/32 , C12N9/02 , C12N9/10 , C12N15/63 , C12N15/82 , A01H5/00 , A01H1/02 , A01H1/04 , A01H5/10 , C12N15/29 , C12N15/31 , C12N15/32 , C12N15/55
CPC分类号： C07K14/32 , C12N15/63 , C12N15/8289 , C12N9/0089 , C12N9/1007 , C07K2319/00 , Y10S47/01
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which neutralizes the activity of another product which disrupts the metabolism, functioning and/or development selectively of the plant's flower cells, particularly reproductive organ cells, or seed cells or embryo cells. The foreign DNA sequence also optionally encodes a marker.
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公开(公告)号：US06627799B1
公开(公告)日：2003-09-30
申请号：US08485516
申请日：1995-06-07
IPC分类号： C12N1582
CPC分类号： C12Y302/01031 , C07K14/415 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12Y301/27003
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning and/or development of stamen cells of the plant. The foreign DNA sequence also optionally encodes a marker.
摘要翻译： 一种植物，其核基因组用编码选择性地破坏植物雄蕊细胞的代谢，功能和/或发育的产物的外源DNA序列转化。 外来DNA序列还任选地编码标记物。
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公开(公告)号：US06320097B1
公开(公告)日：2001-11-20
申请号：US08485788
申请日：1995-06-07
IPC分类号： A01H102
CPC分类号： C12Y302/01031 , C07K14/415 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12Y301/27003
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning and/or development of stamen cells of the plant. The foreign DNA sequence also optionally encodes a marker.
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公开(公告)号：US6046382A
公开(公告)日：2000-04-04
申请号：US21785
申请日：1998-02-10
申请人： Celestina Mariani , Jan Leemans , Willy De Greef
发明人： Celestina Mariani , Jan Leemans , Willy De Greef
IPC分类号： A01H5/00 , A01H1/00 , A01H1/02 , A01H5/10 , C07K14/32 , C12N20060101 , C12N5/00 , C12N5/04 , C12N5/10 , C12N5/14 , C12N9/02 , C12N9/10 , C12N15/00 , C12N15/09 , C12N15/29 , C12N15/31 , C12N15/53 , C12N15/54 , C12N15/62 , C12N15/63 , C12N15/82 , C12R1/91
CPC分类号： C12N9/0089 , C07K14/32 , C12N15/63 , C12N15/8289 , C12N9/1007 , C07K2319/00
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which neutralizes the activity of another product which disrupts the metabolism, functioning and/or development selectively of the plant's flower cells, particularly reproductive organ cells, or seed cells or embryo cells. The foreign DNA sequence also optionally encodes a marker.
摘要翻译： 一种植物，其核基因组用编码产物的外源DNA序列转化，所述产物中和另一种产物的活性，所述产物破坏植物花细胞，特别是生殖器官细胞或种子细胞的选择性代谢，功能和/或发育 或胚胎细胞。 外来DNA序列还任选地编码标记物。
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公开(公告)号：US5652354A
公开(公告)日：1997-07-29
申请号：US485792
申请日：1995-06-07
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , C07K14/415 , C12N5/10 , C12N9/10 , C12N9/22 , C12N9/24 , C12N9/50 , C12N15/09 , C12N15/29 , C12N15/55 , C12N15/56 , C12N15/57 , C12N15/82 , C12N5/04
CPC分类号： C12Y302/01031 , C07K14/415 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12Y301/27003
摘要： Promoters from endogenous genes of plants are isolated, wherein said promoters direct gene expression selectively in stamen cells of said plant, particularly in tapetum cells of said plant. The promoters may be used to transform a plant with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning, and/or development of stamen cells of the plant.
摘要翻译： 分离来自植物内源基因的启动子，其中所述启动子在所述植物的雄蕊细胞中特别是所述植物的绒毡层细胞中选择性地直接进行基因表达。 启动子可用于转化具有编码选择性地破坏植物雄蕊细胞的代谢，功能和/或发育的产物的外源DNA序列的植物。
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公开(公告)号：US06372967B1
公开(公告)日：2002-04-16
申请号：US08027580
申请日：1993-03-05
IPC分类号： A01H102
CPC分类号： C12Y302/01031 , C07K14/415 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12Y301/27003
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a protein or polypeptide which disrupts the metabolism, functioning and/or development of stamen cells of the plant, said foreign DNA under the control of a stamen-specific promoter. The foreign DNA sequence also optionally encodes a marker.
摘要翻译： 一种植物，其核基因组用编码破坏植物雄蕊细胞的代谢，功能和/或发育的蛋白质或多肽的外源DNA序列转化，所述外源DNA在雄蕊特异性启动子的控制下。 外来DNA序列还任选地编码标记物。
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