公开(公告)号：US20110033842A1

公开(公告)日：2011-02-10

申请号：US12935970

申请日：2008-04-04

申请人： Woo Chul Moon ,  Jin Yung Lee ,  Keun Yang Park ,  Jung Sik Shing

发明人： Woo Chul Moon ,  Jin Yung Lee ,  Keun Yang Park ,  Jung Sik Shing

IPC分类号： C12Q1/70 ,  C07H1/06 ,  C12P19/34 ,  C12Q1/68 ,  C12M1/00 ,  A61B10/00

CPC分类号： A61B10/02

摘要： The present invention relates to a new skin gene card for genetic test, a method for acquiring DNA and RNA and performing various genetic tests using the same, and practical applications thereof. More specifically, the inventors of the present invention have developed a skin gene card capable of acquiring samples from human skin, hair or mucosa simply, safely and quickly and enabling stable long-term storage and transport of DNA and RNA included in the acquired sample at room temperature. Various genetic tests may performed using the acquired DNA and RNA, including polymerase chain reaction (PCR), reverse transcription (RT)-PCR, real-time PCR, sequencing, hybridization, DNA chip analysis, single-nucleotide polymorphism (SNP) assay, gene mutation assay, promoter methylation assay, gene expression assay, etc. The genetic skin test result may be utilized for disease prognosis, nutrigenomic test, pharmacogenomic test, forensic test such as personal identification, diagnosis of genetic diseases, diagnosis of skin diseases, or the like. In addition, through an objective evaluation of the skin or hair condition, a personalized cosmetic and skin care system may be established for practical application in beauty care, cosmetology, dermatology, and clinical practice.

摘要翻译： 本发明涉及一种用于遗传检测的新皮肤基因卡，一种获取DNA和RNA并使用该皮肤基因卡进行各种遗传检测的方法及其实际应用。 更具体地说，本发明的发明人开发出能够简单，安全，快速地从人体皮肤，毛发或粘膜中获取样品的皮肤基因卡，并使获得的样品中包含的DNA和RNA能够稳定地长期储存和转运 室内温度。 使用获得的DNA和RNA进行各种遗传检测，包括聚合酶链反应（PCR），逆转录（RT）-PCR，实时PCR，测序，杂交，DNA芯片分析，单核苷酸多态性（SNP） 基因突变测定，启动子甲基化测定，基因表达测定等。遗传性皮肤试验结果可用于疾病预后，营养基因组检测，药物基因组学检测，法医检验，如个人鉴定，遗传疾病诊断，皮肤病诊断或 类似。 此外，通过对皮肤或毛发状况的客观评价，可以建立个性化的化妆品和护肤系统，用于美容护理，美容，皮肤病学和临床实践中的实际应用。

公开(公告)号：US20130184164A1

公开(公告)日：2013-07-18

申请号：US13704942

申请日：2010-06-25

申请人： Woo Chul Moon ,  Myung Ryurl Oh

发明人： Woo Chul Moon ,  Myung Ryurl Oh

IPC分类号： C12Q1/70

CPC分类号： C12Q1/708 ,  C12Q1/6837 ,  C12Q1/6886

摘要： Disclosed is a DNA chip (or DNA microarray) on which probes complementarily binding to the nucleic acids of 44 types of HPV, which is the main cause of cervical cancer and the most common cause of sexually transmitted diseases, are spotted, a genotyping kit including same and a genotyping method using same. In accordance with the present disclosure, all the 44 types of HPV invading the genitalia can be detected and coinfection by more than one type of HPV can be diagnosed accurately. The sensitivity and specificity of HPV genotyping is close to 100% and a number of samples can be tested quickly. The present disclosure is very useful in predicting cervical cancer and precancerous lesions.

摘要翻译： 公开了一种DNA芯片（或DNA微阵列），其中探测到与宫颈癌的主要原因和最常见的性传播疾病的44种类型的HPV的核酸互补结合的探针，基因分型试剂盒包括 相同的和使用它的基因分型方法。 根据本公开，可以检测所有44种HPV侵入生殖器，并且可以准确地诊断多于一种类型的HPV的共感染。 HPV基因分型的敏感性和特异性接近100％，许多样品可以快速测试。 本公开在预测子宫颈癌和癌前病变中非常有用。

公开(公告)号：US20130237427A1

公开(公告)日：2013-09-12

申请号：US13581371

申请日：2010-03-26

申请人： Woo Chul Moon ,  Myung Ryurl Oh

发明人： Woo Chul Moon ,  Myung Ryurl Oh

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6886 ,  C12Q1/6888 ,  C12Q1/701 ,  C12Q1/708 ,  C12Q2600/156 ,  C12Q2565/543

摘要： The present disclosure relates to a Y-shaped nucleotide probe having two probe parts in one body, which provides improved sensitivity, specificity and accuracy in genotype and genetic analysis and thus is widely applicable to diagnosis and a variant thereof (d- or b-shaped probe), and a DNA microarray, a kit and a genetic analysis method using the same. The Y-shaped probe comprises a left-side probe part, a left-side stem part, a linker part, a right-side stem part and a right-side probe part. The DNA microarray of the present disclosure can improve accuracy of test by testing the same gene simultaneously or by testing two different target genes at once. Especially, since both the target gene and the control gene can be tested simultaneously from one spot, error can be reduced, quantitative analysis is possible and standardization is easy. The Y-shaped probe of the present disclosure may be widely used for genotyping, analysis of gene expression, analysis of mutation or SNP, diagnosis and prediction of diseases, determination of therapeutic regimen, or the like.

摘要翻译： 本公开内容涉及在一个体内具有两个探针部分的Y型核苷酸探针，其在基因型和遗传分析中提供改善的灵敏度，特异性和准确性，因此广泛适用于诊断及其变体（d-或b-型 探针）和DNA微阵列，试剂盒和使用其的遗传分析方法。 Y形探针包括左侧探针部分，左侧杆部分，连接部分，右侧杆部分和右侧探针部分。 本公开的DNA微阵列可以通过同时测试相同的基因或通过一次测试两种不同的靶基因来提高测试的准确性。 特别是，既可以从一个点同时测试靶基因和对照基因，也可以减少误差，可以进行定量分析，容易进行标准化。 本公开的Y型探针可广泛用于基因分型，基因表达分析，突变或SNP分析，疾病的诊断和预测，治疗方案的确定等。

公开(公告)号：US20120004113A1

公开(公告)日：2012-01-05

申请号：US13131207

申请日：2008-12-17

申请人： Woo Chul Moon

发明人： Woo Chul Moon

IPC分类号： C40B20/00 ,  C40B40/06

CPC分类号： C12Q1/689 ,  C12Q1/6837 ,  C12Q2600/156 ,  C12Q2600/16

摘要： Disclosed are a DNA chip and a kit capable of quickly and accurately detecting or genotyping the highly prevalent and important eleven microbes causing sexually transmitted diseases (STD) Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, syphilis-causing treponema, pallidum, chancroid-causing Haemophilus ducreyi, genital herpes-causing herpes simplex virus 1 and 2, human papillomavirus (HPV) and Trichomonas vaginalis and three related organisms Candida albicans, Gardnerella vaginalis and coliform bacteria and analyzing antibiotic resistance against tetracycline and lactam antibiotics, and a method for detecting or genotyping using the same. According to the present invention, the presence, genotype and antibiotic resistance of the fourteen organisms can be analyzed quickly and accurately from a DNA sample. With excellent sensitivity, specificity, reproducibility and accuracy of the 14 STD-causing and related microorganisms may be automatically identified quickly and accurately from multiple samples, and selection of antibiotics may be aided.

摘要翻译： 公开了能够快速且准确地检测或基因分型引起性传播疾病（STD）淋病奈瑟氏球菌，沙眼衣原体，解脲支原体，生殖支原体，人支原体，梅毒引起的密螺旋体的DNA芯片和试剂盒， 苍白球，致引起流感嗜血杆菌，生殖器疱疹单纯疱疹病毒1和2，人乳头状瘤病毒（HPV）和阴道毛滴虫和三种相关生物体白色念珠菌，阴道加德纳菌和大肠杆菌，分析抗四环素和内酰胺抗生素的抗生素抗性， 一种使用其检测或基因分型的方法。 根据本发明，可以从DNA样品中快速准确地分析14种生物体的存在，基因型和抗生素抗性。 具有良好的灵敏度，特异性，重现性和14 STD引起和相关微生物的准确性可以从多个样品中快速准确地自动识别，并且可以帮助选择抗生素。