公开(公告)号：US10584362B2

公开(公告)日：2020-03-10

申请号：US15303944

申请日：2014-07-30

Applicant: Xiamen University

Inventor： Ning He ,  Haosheng Yao ,  Yu Liu ,  Wencheng Yu ,  Shan Yan ,  Yuanpeng Wang ,  Qingbiao Li

IPC: C12P19/04 ,  C12N1/20 ,  C12P21/02 ,  C07K14/32

Abstract: The present application provides a method for simultaneously synthesizing a biological flocculant with polysaccharide and γ-PGA as the active components by using Bacillus licheniformis. Bacillus licheniformis is inoculated to a slant culture medium to be cultured; a single colony on a fresh plate is inoculated to a seed culture medium to be cultured; and a seed fermentation broth is inoculated to a fermentation culture medium to be cultured, and then the biological flocculant having two different components is obtained. The flocculant synthesized in the present invention is high in activity and good in thermal stability; and especially, the flocculation effect of polysaccharide is relatively superior under acidic and neutral conditions, and the flocculation activity of γ-PGA is relatively higher under neutral and alkaline environments, which can satisfy a relatively large pH application range.

公开(公告)号：US20170029855A1

公开(公告)日：2017-02-02

申请号：US15303944

申请日：2014-07-30

Applicant: Xiamen University

Inventor： Ning He ,  Haosheng Yao ,  Yu Liu ,  Wencheng Yu ,  Shan Yan ,  Yuanpeng Wang ,  Qingbiao Li

IPC: C12P19/04 ,  C07K14/32

CPC classification number: C12P19/04 ,  C07K14/32 ,  C12N1/20 ,  C12P21/02

Abstract: The present application provides a method for simultaneously synthesizing a biological flocculant with polysaccharide and γ-PGA as the active components by using Bacillus licheniformis. Bacillus licheniformis is inoculated to a slant culture medium to be cultured; a single colony on a fresh plate is inoculated to a seed culture medium to be cultured; and a seed fermentation broth is inoculated to a fermentation culture medium to be cultured, and then the biological flocculant having two different components is obtained. The flocculant synthesized in the present invention is high in activity and good in thermal stability; and especially, the flocculation effect of polysaccharide is relatively superior under acidic and neutral conditions, and the flocculation activity of γ-PGA is relatively higher under neutral and alkaline environments, which can satisfy a relatively large pH application range.

Abstract translation: 本申请提供了一种通过使用地衣芽孢杆菌同时合成生物絮凝剂与多糖和γ-PGA作为活性成分的方法。 将地衣芽孢杆菌接种到待培养的倾斜培养基中; 将新鲜板上的单个菌落接种到待培养的种子培养基中; 将种子发酵液接种到待培养的发酵培养基中，得到具有两种不同成分的生物絮凝剂。 本发明合成的絮凝剂活性高，热稳定性好; 特别是在酸性和中性条件下，多糖的絮凝效果相对较好，中性和碱性环境下γ-PGA的絮凝活性相对较高，可以满足较大的pH应用范围。

公开(公告)号：US20170044474A1

公开(公告)日：2017-02-16

申请号：US15306398

申请日：2014-07-30

Applicant: XIAMEN UNIVERSITY

Inventor： Qingbiao Li ,  Xi Chen ,  Ning He ,  Yuanpeng Wang ,  Liang Shen ,  Haitao Wang

IPC: C12M1/00 ,  C02F3/12 ,  C12M1/12 ,  C02F1/44 ,  B01D69/08 ,  C02F3/32 ,  C02F3/10

CPC classification number: C12M21/02 ,  B01D63/02 ,  B01D69/08 ,  B01D2311/2688 ,  C02F1/44 ,  C02F3/102 ,  C02F3/1268 ,  C02F3/1273 ,  C02F3/322 ,  C02F2101/105 ,  C02F2101/16 ,  C02F2103/22 ,  C02F2203/006 ,  C12M21/04 ,  C12M23/58 ,  C12M25/10 ,  C12M29/00 ,  C12M29/16 ,  Y02E50/343 ,  Y02W10/15 ,  Y02W10/37

Abstract: A membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in a biogas slurry and treating method thereof, relating to biogas slurry treatment. The membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in a biogas slurry is provided with a biogas slurry storage tank, peristaltic pumps, a microalgae cultivating tank, an air pump, a membrane photobioreactor and a hollow fiber membrane. The biogas slurry containing nitrogen and phosphorus that are out of limits is stored in the biogas slurry storage tank, and is driven by a first peristaltic pump to circularly flow in a silicone pipe; a microalgae solution is cultivated under illumination in the microalgae cultivating tank, and is driven by a second peristaltic pump to circularly flow in a silicone pipe, air is fed into the microalgae cultivating tank through the air pump, the biogas slurry and the microalgae solution are converged in the membrane photobioreactor, and the biogas slurry circularly flows inside the hollow fiber membrane pipe and the microalgae solution circularly flows outside the hollow fiber membrane pipe, the two being in a cross flow; and the nitrogen and phosphorus that are out of limits in the biogas slurry penetrate from the inside of the hollow fiber membrane and are absorbed by the microalgae solution outside the membrane, and after cyclical cultivation, nitrogen and phosphorus that are out of limits in the biogas slurry are absorbed, and the discharge standards are achieved.

Abstract translation: 用于处理生物气浆中的氮和磷的限制的膜光生物反应器及其处理方法，涉及沼气处理。 用于处理生物气体浆液中不合格的氮磷的膜光生物反应器具有生物气浆料储罐，蠕动泵，微藻培养槽，空气泵，膜光生物反应器和中空纤维膜。 含有氮，磷的沼气浆料储存在沼气储存槽内，由第一蠕动泵驱动，在硅胶管中循环流动; 在微藻培养罐中照射微藻溶液，由第二蠕动泵驱动，在硅胶管中循环流动，空气通过空气泵进入微藻培养罐，沼液和微藻溶液为 汇聚在膜光生物反应器中，沼气浆液在中空纤维膜管内循环流动，微藻溶液循环流过中空纤维膜管，二者呈横流; 沼气浆中脱氮的氮磷从中空纤维膜的内部渗透，被膜外的微藻溶液吸收，经过循环栽培，沼气不合格的氮磷， 浆料被吸收，达到排放标准。

公开(公告)号：US11505656B2

公开(公告)日：2022-11-22

申请号：US16374464

申请日：2019-04-03

Applicant: XIAMEN UNIVERSITY

Inventor： Liming Che ,  Xiang Li ,  Ning He ,  Xue'e Wu ,  Yuanpeng Wang ,  Xueping Ling

IPC: C08J3/075 ,  A61L27/52 ,  A61L27/20 ,  A61K47/36 ,  A61K31/353 ,  A61K38/38 ,  A61K31/375 ,  A61K35/747 ,  A61K35/00

Abstract: The invention relates to a method for embedding a load based on gel high hydrostatic pressure liquefaction. Using the phenomenon that the physical gel is liquefied under high pressure, the vacuum-packaged high-methoxyl pectin gel is treated under a pressure of 400-600 MPa for 5-30 min, mixed with the load, and then subjected to a pressure of 400-600 MPa for homogenization treatment for 5 to 30 min. After pressure relief, the liquefied gel is poured into a mold for reshaping, followed by removal of free water and coating treatment. This method combines the advantages of high hydrostatic pressure technology in modification and sterilization. It has mild embedding conditions and wide sources of raw materials to prepare the carrier, which has excellent biocompatibility and biodegradability. It can be widely used for embedding microorganisms, enzymes, proteins and small molecular substances. The loaded gel prepared by the method has high microbial safety, can effectively maintain the activity of the load. The load distribution is uniform, and the load amount is much larger than the traditional adsorption load.

公开(公告)号：US10533148B2

公开(公告)日：2020-01-14

申请号：US15306398

申请日：2014-07-30

Applicant: XIAMEN UNIVERSITY

Inventor： Qingbiao Li ,  Xi Chen ,  Ning He ,  Yuanpeng Wang ,  Liang Shen ,  Haitao Wang

IPC: C12M1/00 ,  B01D69/08 ,  C02F3/12 ,  C02F3/32 ,  C12M1/12 ,  C02F101/10 ,  C02F101/16 ,  C02F1/44 ,  C02F3/10 ,  C02F103/22

Abstract: A membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in a biogas slurry and treating method thereof, relating to biogas slurry treatment. The membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in a biogas slurry is provided with a biogas slurry storage tank, peristaltic pumps, a microalgae cultivating tank, an air pump, a membrane photobioreactor and a hollow fiber membrane. The biogas slurry containing nitrogen and phosphorus that are out of limits is stored in the biogas slurry storage tank, and is driven by a first peristaltic pump to circularly flow in a silicone pipe; a microalgae solution is cultivated under illumination in the microalgae cultivating tank, and is driven by a second peristaltic pump to circularly flow in a silicone pipe, air is fed into the microalgae cultivating tank through the air pump, the biogas slurry and the microalgae solution are converged in the membrane photobioreactor, and the biogas slurry circularly flows inside the hollow fiber membrane pipe and the microalgae solution circularly flows outside the hollow fiber membrane pipe, the two being in a cross flow; and the nitrogen and phosphorus that are out of limits in the biogas slurry penetrate from the inside of the hollow fiber membrane and are absorbed by the microalgae solution outside the membrane, and after cyclical cultivation, nitrogen and phosphorus that are out of limits in the biogas slurry are absorbed, and the discharge standards are achieved.