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公开(公告)号：US07741534B2
公开(公告)日：2010-06-22
申请号：US12051893
申请日：2008-03-20
申请人： Xiaomu Niu , Haiyin Wang , Dwight T Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T Tomes , Sabine Hantke , Norbert Brugiere
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素诱导能力的启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20100325752A1
公开(公告)日：2010-12-23
申请号：US12819888
申请日：2010-06-21
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions and methods include expression cassettes and transformed plants and provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物和方法包括表达盒和转化植物，并提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20080172760A1
公开(公告)日：2008-07-17
申请号：US12051893
申请日：2008-03-20
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素诱导能力的启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20060037103A1
公开(公告)日：2006-02-16
申请号：US11074144
申请日：2005-03-07
申请人： Xiaomu Niu , Haiyin Wang , Dwight Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight Tomes , Sabine Hantke , Norbert Brugiere
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin. inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素的启动子的新型核酸序列。 诱导性。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20090188003A1
公开(公告)日：2009-07-23
申请号：US12358698
申请日：2009-01-23
CPC分类号： C12N15/8273 , C07K14/415 , C07K14/4705 , C12N15/8271
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel polypeptides involved in modulating gene expression in response to abiotic stress such as cold or drought, and the polynucleotides encoding the polypeptides. Methods for expressing the polynucleotides in a plant and improving cold and/or drought tolerance of plants are also provided.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物包括涉及调节基因表达以响应非生物胁迫如寒冷或干旱的新型多肽和编码多肽的多核苷酸。 还提供了用于在植物中表达多核苷酸并改善植物的冷和/或耐旱性的方法。
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公开(公告)号：US07553951B2
公开(公告)日：2009-06-30
申请号：US11669281
申请日：2007-01-31
申请人： Norbert Brugiere
发明人： Norbert Brugiere
CPC分类号： C12N15/8295 , C12N9/1085 , C12N15/52 , C12N15/8261 , C12N15/8262 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Polynucleotide sequences encoding isopentenyl transferase (IPT) polypeptides are provided, as are the amino acid sequences of the encoded polypeptides. The sequences can be used in a variety of methods including modulating root development, modulating floral development, modulating leaf and/or shoot development, modulating senescence, modulating seed size and/or weight, and modulating tolerance of plants to abiotic stress. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译： 提供了调节植物发育的方法和组合物。 提供编码异戊烯基转移酶（IPT）多肽的多核苷酸序列，以及编码多肽的氨基酸序列。 该序列可用于各种方法，包括调节根发育，调节花发育，调节叶和/或芽发育，调节衰老，调节种子大小和/或重量，以及调节植物对非生物胁迫的耐受性。 还提供了转化的植物，植物细胞，组织和种子。
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7.发明申请DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING MATE-EFFLUX POLYPEPTIDES 审中-公开耐旱植物及相关构成及相关方法涉及编码MAFFEFFLUX多肽的基因公开(公告)号：US20130269063A1
公开(公告)日：2013-10-10
申请号：US13989838
申请日：2011-12-19
申请人： Stephen M. Allen , Stanley Luck , Jeffrey Mullen , Hajime Sakai , Scott V. Tingey , Robert Wayne Williams , Norbert Brugiere , Ratna Kumria , Amitabh Mohanty , Rupa Raja
发明人： Stephen M. Allen , Stanley Luck , Jeffrey Mullen , Hajime Sakai , Scott V. Tingey , Robert Wayne Williams , Norbert Brugiere , Ratna Kumria , Amitabh Mohanty , Rupa Raja
IPC分类号： C12N15/82
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a MATE-efflux polypeptide.
摘要翻译： 用于赋予耐旱性的分离的多核苷酸和多肽和重组DNA构建体，包含这些重组DNA构建体的组合物（例如植物或种子），以及利用这些重组DNA构建体的方法。 重组DNA构建体包含可操作地连接于在植物中有功能的启动子的多核苷酸，其中所述多核苷酸编码MATE-外排多肽。
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公开(公告)号：US20080189810A1
公开(公告)日：2008-08-07
申请号：US12020910
申请日：2008-01-28
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Methods employing type A Response Regulators (RR) are provided. The type A RR sequences are used in a variety of methods including modulating root development, modulating leaf and/or shoot development, modulating shoot regeneration from callus, modulating tolerance under abiotic stress, modulating yield, modulating cytokinin level/activity, and modulating plant responsiveness to cytokinin. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.
摘要翻译： 提供了调节植物发育的方法和组合物。 提供了使用A型响应调节器（RR）的方法。 A型RR序列用于各种方法，包括调节根发育，调节叶和/或芽发育，调节愈伤组织的芽再生，调节非生物胁迫下的耐受性，调节产量，调节细胞分裂素水平/活性和调节植物反应性 到细胞分裂素。 还提供了转化的植物，植物细胞，组织，种子和表达载体。
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公开(公告)号：US20060168692A1
公开(公告)日：2006-07-27
申请号：US11329868
申请日：2006-01-11
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Methods employing type A Response Regulators (RR) are provided. The type A RR sequences are used in a variety of methods including modulating root development, modulating leaf and/or shoot development, modulating shoot regeneration from callus, modulating tolerance under abiotic stress, modulating yield, modulating cytokinin level/activity, and modulating plant responsiveness to cytokinin. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.
摘要翻译： 提供了调节植物发育的方法和组合物。 提供了使用A型响应调节器（RR）的方法。 A型RR序列用于各种方法，包括调节根发育，调节叶和/或芽发育，调节愈伤组织的芽再生，调节非生物胁迫下的耐受性，调节产量，调节细胞分裂素水平/活性和调节植物反应性 到细胞分裂素。 还提供了转化的植物，植物细胞，组织，种子和表达载体。
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公开(公告)号：US08222483B2
公开(公告)日：2012-07-17
申请号：US12165935
申请日：2008-07-01
申请人： Norbert Brugiere , Jeffrey E Habben
发明人： Norbert Brugiere , Jeffrey E Habben
CPC分类号： C12N15/8222 , C12N9/0032 , C12N15/8261 , C12N15/8271 , C12N15/8295 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Polynucleotide sequences and amino acid sequences encoding cytokinin oxidase polypeptides are provided. The sequences can be used in a variety of methods including modulating root development, modulating floral development, modulating leaf and/or shoot development, modulating seed size and/or weight, modulating tolerance under abiotic stress, and modulating resistance to pathogens. Polynucleotides comprising CKX promoters are also provided. The promoters can be used to regulate expression of a sequence of interest. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译： 提供了调控植物发育的方法和组合物。 提供了编码细胞分裂素氧化酶多肽的多核苷酸序列和氨基酸序列。 该序列可用于各种方法，包括调节根发育，调节花发育，调节叶和/或芽发育，调节种子大小和/或重量，调节非生物胁迫下的耐受性和调节对病原体的抗性。 还提供了包含CKX启动子的多核苷酸。 启动子可用于调节感兴趣序列的表达。 还提供了转化的植物，植物细胞，组织和种子。
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