公开(公告)号：US4959320A

公开(公告)日：1990-09-25

申请号：US763587

申请日：1985-08-08

申请人： Yahiro Uemura ,  Kazumi Fukuyama ,  Takashi Kobayashi ,  Yoshiaki Kanou ,  Ryutaro Yamana ,  Eiji Kashiwagi ,  Tomokuni Taniguchi ,  Kazuaki Nakura ,  Masahiro Watanabe ,  Masayuki Nishida ,  Tadakazu Suyama

发明人： Yahiro Uemura ,  Kazumi Fukuyama ,  Takashi Kobayashi ,  Yoshiaki Kanou ,  Ryutaro Yamana ,  Eiji Kashiwagi ,  Tomokuni Taniguchi ,  Kazuaki Nakura ,  Masahiro Watanabe ,  Masayuki Nishida ,  Tadakazu Suyama

IPC分类号： G01N33/574 ,  A61K38/00 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/30 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC分类号： C07K16/3046 ,  C07K16/303 ,  A61K38/00

摘要： A monoclonal antibody is described, having a specific reactivity with a cancer-associated antigen selected from pancreas cancer-associated antigen, intestinum crassum cancer-associated antigen and hepatoma-associated antigen and has the following characteristics:(1) it exists in a blood serum of pancreas cancer, intestinum crassum cancer and hepatoma patients;(2) it comprises glycoproteins;(3) it has a molecular weight of about 700,000 to 1,500,000, as measured by gel-filtration and determined by comparison with that of a known protein;(4) it has a maximum absorption of 2800 nm when dissolved in 0.1 M acetic acid buffer solution (pH 4.5); and(5) it is released at least in a cultured supernatant medium of establihed cell line of COLO-201, TE-1, TE-2, TE-3, NRC-12, MKN-45 and KATO III. The monoclonal antibody is utilized as a reagent for detecting pancreas cancer, intestinum crassum cancer and hepatoma. A process for preparing the monoclonal antibody and a process for preparing the reagent are also disclosed.

摘要翻译： 描述了与选自胰腺癌相关抗原，肠um癌相关抗原和肝癌相关​​抗原的癌相关抗原具有特异性反应性的单克隆抗体，具有以下特征：（1）其存在于血清 的胰腺癌，肠um癌和肝癌患者; （2）它包含糖蛋白; （3）通过凝胶过滤测定，其分子量约为700,000至1,500,000，并与已知蛋白质的比较确定; （4）当溶于0.1M乙酸缓冲溶液（pH 4.5）时，其最大吸收为2800nm; 至少在COLO-201，TE-1，TE-2，TE-3，NRC-12，MKN-45和KATO III的建立细胞系的培养上清培养基中释放。 单克隆抗体用作检测胰腺癌，肠um癌和肝癌的试剂。 还公开了制备单克隆抗体的方法和制备该试剂的方法。