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    Ethanol extract of Antrodia camphorata for inducing apoptosis and preparation method thereof
    1.
    发明授权
    Ethanol extract of Antrodia camphorata for inducing apoptosis and preparation method thereof 有权
    用于诱导凋亡的樟脑酸乙醇提取物及其制备方法

    公开(公告)号：US08883167B2

    公开(公告)日：2014-11-11

    申请号：US13398368

    申请日：2012-02-16

    申请人： Yang-Chang Wu ,  Mei-Chin Lu ,  Fang-Rong Chang ,  Ying-Chi Du ,  Tung-Ying Wu

    发明人： Yang-Chang Wu ,  Mei-Chin Lu ,  Fang-Rong Chang ,  Ying-Chi Du ,  Tung-Ying Wu

    IPC分类号： A61K36/07

    CPC分类号： A61K36/07 ,  Y10T436/216

    摘要： A preparation method for an ethanol extract of the fruiting body of Antrodia camphorata (EEAC) is provided. The preparation method includes steps of: (a) providing the fruiting body of A. camphorata (AC); (b) extracting the fruiting bodies with a first ethanol solution; and (c) obtaining EEAC. EEAC further can be sequentially extracted or fractioned by n-hexane, ethyl acetate and ethanol, and an n-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated. The growth inhibition and apoptosis induction of leukemia cell line HL 60 are effectively mediated by FA product, in which zhankuic acid A is the bioactive marker. The amount of triterpenoid in the fruiting body of AC can be determined by NMR and HPLC analysis.

    摘要翻译： 本发明提供了樟树（梧桐）子实体的乙醇提取物的制备方法。 该制备方法包括以下步骤：（a）提供樟芝（AC）的子实体; （b）用第一乙醇溶液萃取子实体; 和（c）获得EEAC。 EEAC还可以通过正己烷，乙酸乙酯和乙醇依次萃取或分馏，分别生成正己烷馏分（FC），乙酸乙酯馏分（FA）和乙醇馏分（FB）。 白血病细胞系HL60的生长抑制和凋亡诱导由FA产物有效介导，其中zh ic酸A是生物活性标记物。 AC的子实体中三萜类化合物的量可以通过NMR和HPLC分析确定。

    ETHANOL EXTRACT OF ANTRODIA CAMPHORATA FOR INDUCING APOPTOSIS AND PREPARATION METHOD THEREOF
    2.
    发明申请
    ETHANOL EXTRACT OF ANTRODIA CAMPHORATA FOR INDUCING APOPTOSIS AND PREPARATION METHOD THEREOF 有权
    用于诱导蚜虫的蒽醌提取物及其制备方法

    公开(公告)号：US20120141524A1

    公开(公告)日：2012-06-07

    申请号：US13398368

    申请日：2012-02-16

    申请人： Yang-Chang Wu ,  Mei-Chin Lu ,  Fang-Rong Chang ,  Ying-Chi Du ,  Tung-Ying Wu

    发明人： Yang-Chang Wu ,  Mei-Chin Lu ,  Fang-Rong Chang ,  Ying-Chi Du ,  Tung-Ying Wu

    IPC分类号： A61K36/07 ,  A61P35/02 ,  G01N30/00 ,  A61K31/575

    CPC分类号： A61K36/07 ,  Y10T436/216

    摘要： A preparation method for an ethanol extract of the fruiting body of Antrodia camphorata (EEAC) is provided. The preparation method includes steps of: (a) providing the fruiting body of A. camphorata (AC); (b) extracting the fruiting bodies with a first ethanol solution; and (c) obtaining EEAC. EEAC further can be sequentially extracted or fractioned by n-hexane, ethyl acetate and ethanol, and an n-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated. The growth inhibition and apoptosis induction of leukemia cell line HL 60 are effectively mediated by FA product, in which zhankuic acid A is the bioactive marker. The amount of triterpenoid in the fruiting body of AC can be determined by NMR and HPLC analysis.

    摘要翻译： 本发明提供了樟树（梧桐）子实体的乙醇提取物的制备方法。 该制备方法包括以下步骤：（a）提供樟芝（AC）的子实体; （b）用第一乙醇溶液萃取子实体; 和（c）获得EEAC。 EEAC还可以通过正己烷，乙酸乙酯和乙醇依次萃取或分馏，分别生成正己烷馏分（FC），乙酸乙酯馏分（FA）和乙醇馏分（FB）。 白血病细胞系HL60的生长抑制和凋亡诱导由FA产物有效介导，其中zh ic酸A是生物活性标记物。 AC的子实体中三萜类化合物的量可以通过NMR和HPLC分析确定。

    Benzenoid compounds of antrodia cinnamomea, preparation and analysis method thereof
    3.
    发明授权
    Benzenoid compounds of antrodia cinnamomea, preparation and analysis method thereof 有权
    桂皮酚的苯并噻吩类化合物，其制备及分析方法

    公开(公告)号：US08716499B2

    公开(公告)日：2014-05-06

    申请号：US13346300

    申请日：2012-01-09

    申请人： Yang-Chang Wu ,  Fang-Rong Chang ,  Mei-Chin Lu ,  Ying-Chi Du ,  Tung-Ying Wu ,  Yu-Ming Hsu

    发明人： Yang-Chang Wu ,  Fang-Rong Chang ,  Mei-Chin Lu ,  Ying-Chi Du ,  Tung-Ying Wu ,  Yu-Ming Hsu

    IPC分类号： C07D317/48

    CPC分类号： C07D317/64 ,  C07C41/38 ,  G01N2030/8813 ,  C07C43/215

    摘要： Disclosed are a method for preparing an n-hexane extract of the fruiting body of Antrodia cinnamomea (AC), wherein the fruiting body of AC is sequentially extracted with the ethanol solution and the n-hexane solution to obtain the n-hexane extract containing at least one benzenoid compound. The amounts of 4,7-dimethoxy-5-(3-methylbut-3-en-1-ynyl)-6-methyl-1,3-benzodioxole, 4,7-dimethoxy-5-methyl-1,3-benzodioxole, antrocamphine A and the combination thereof in the at least one benzenoid compound are determined using chromatography, NMR and HPLC. In addition, the present invention is applicable on detecting the amounts of benzenoid compounds in the AC healthcare food/drug or the fruiting body of AC, and thus owns the industrial values.

    摘要翻译： 本发明提供了一种用于制备仙人掌（AC）子实体的正己烷提取物的方法，其中AC的子实体依次用乙醇溶液和正己烷溶液萃取，得到含有 至少一种苯类化合物。 4,7-二甲氧基-5-（3-甲基丁-3-烯-1-炔基）-6-甲基-1,3-苯并间二氧杂环戊烯，4,7-二甲氧基-5-甲基-1,3-苯并间二氧杂环戊烯 ，抗阿奇霉素A及其在至少一种苯类化合物中的组合使用色谱法，NMR和HPLC测定。 此外，本发明可应用于检测AC保健食品/药物或AC的子实体中的苯类化合物的量，因此具有工业价值。

    ETHANOL EXTRACT OF ANTRODIA CAMPHORATA FOR INDUCING APOPTOSIS AND PREPARATION METHOD THEREOF
    5.
    发明申请
    ETHANOL EXTRACT OF ANTRODIA CAMPHORATA FOR INDUCING APOPTOSIS AND PREPARATION METHOD THEREOF 审中-公开
    用于诱导蚜虫的蒽醌提取物及其制备方法

    公开(公告)号：US20100210869A1

    公开(公告)日：2010-08-19

    申请号：US12538549

    申请日：2009-08-10

    申请人： Yang-Chang Wu ,  Mei-Chin Lu ,  Fang-Rong Chang ,  Ying-Chi Du ,  Tung-Ying Wu

    发明人： Yang-Chang Wu ,  Mei-Chin Lu ,  Fang-Rong Chang ,  Ying-Chi Du ,  Tung-Ying Wu

    IPC分类号： C07C69/003 ,  C07C29/74 ,  G01N33/00

    CPC分类号： A61K36/07 ,  Y10T436/216

    摘要： A preparation method for an ethanol extract of the fruiting body of Antrodia camphorata (EEAC) is provided. The preparation method includes steps of: (a) providing the fruiting body of A. camphorata (AC); (b) extracting the fruiting bodies with a first ethanol solution; and (c) obtaining EEAC. EEAC further can be sequentially extracted or fractioned by n-hexane, ethyl acetate and ethanol, and an n-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated. The growth inhibition and apoptosis induction of leukemia cell line HL 60 are effectively mediated by FA product, in which zhankuic acid A is the bioactive marker. The amount of triterpenoid in the fruiting body of AC can be determined by NMR and HPLC analysis.

    摘要翻译： 本发明提供了樟树（梧桐）子实体的乙醇提取物的制备方法。 该制备方法包括以下步骤：（a）提供樟芝（AC）的子实体; （b）用第一乙醇溶液萃取子实体; 和（c）获得EEAC。 EEAC还可以通过正己烷，乙酸乙酯和乙醇依次萃取或分馏，分别生成正己烷馏分（FC），乙酸乙酯馏分（FA）和乙醇馏分（FB）。 白血病细胞系HL60的生长抑制和凋亡诱导由FA产物有效介导，其中zh ic酸A是生物活性标记物。 AC的子实体中三萜类化合物的量可以通过NMR和HPLC分析确定。