公开(公告)号：US07138241B2

公开(公告)日：2006-11-21

申请号：US10146816

申请日：2002-05-17

申请人： Yasuhiro Goda ,  Ayako Kobayashi ,  Masato Hirobe

发明人： Yasuhiro Goda ,  Ayako Kobayashi ,  Masato Hirobe

IPC分类号： G01N33/53 ,  C07K16/00

CPC分类号： C07K16/26 ,  G01N33/6854

摘要： The present invention provides for a method of selecting an antibody against a target substance to be measured, which comprises selecting the antibody against the target substance by antigen-antibody reaction in the presence of a substance interfering with the antigen-antibody reaction. That is, an antigen and a labeled antigen are reacted with the antibody in the presence of an interfering substance, such as an environment pollutant, and on the basis of the degree of reaction thereof, the antibody against the target substance, which is highly resistant to the interfering substance, is selected. Thereby, even if a test sample is contaminated with a substance interfering with antigen-antibody reaction, the antibody, which is highly resistant to the substance interfering with antigen-antibody reaction, is not influenced by the interfering substance and gives a correct value in the quantification. The present invention further provides for antibodies, which are highly resistant to the substance interfering with the antigen-antibody reaction, and hybridomas producing the antibodies.

摘要翻译： 本发明提供了选择抗被测物质的抗体的方法，该方法包括在干扰抗原 - 抗体反应的物质存在下通过抗原 - 抗体反应选择针对目标物质的抗体。 也就是说，抗原和标记的抗原在抗干扰物质如环境污染物的存在下，与抗体反应，并且基于其抗高度耐药的靶物质的抗体的反应程度 对干扰物质的选择。 因此，即使测试样品被干扰抗原抗体反应的物质污染，对干扰抗原反应的物质高度抗性的抗体也不受干扰物质的影响，并且在 量化。 本发明进一步提供对干扰抗原 - 抗体反应的物质高抗性的抗体和产生抗体的杂交瘤。

公开(公告)号：US20070072234A1

公开(公告)日：2007-03-29

申请号：US10553305

申请日：2004-04-13

申请人： Norihiro Kobayashi ,  Yasuhiro Goda ,  Masato Hirobe

发明人： Norihiro Kobayashi ,  Yasuhiro Goda ,  Masato Hirobe

IPC分类号： G01N33/53 ,  C07K14/47 ,  C07K16/18

CPC分类号： C07K14/47 ,  G01N33/1826

摘要： The present invention provides a protein having a binding capacity to a plasticizer, which has been conferred with useful properties for measuring, quantifying, or concentrating a plasticizer, such as high sensitivity, low cross-reactivity, high tolerance for interferents, and high tolerance for solvents. Specifically, the present invention provides a modified protein having various properties, such as affinity for antigenic plasticizers, antigen binding capacity, cross-reactivity, tolerance for antigen-antibody reaction interferents, tolerance for enzymatic color developing reaction interferents, and tolerance for solvents, improved by gene recombination technology.

摘要翻译： 本发明提供了具有对增塑剂的结合能力的蛋白质，其具有用于测量，定量或浓缩增塑剂的有用性质，例如高灵敏度，低交叉反应性，对干扰物的高耐受性和高耐受性 溶剂。 具体而言，本发明提供具有各种性质的修饰蛋白质，例如抗原增塑剂的亲和性，抗原结合能力，交叉反应性，抗原抗体反应干扰物的耐受性，酶促显色反应干扰物的耐受性和耐溶剂性，改进 通过基因重组技术。