公开(公告)号：US07138241B2

公开(公告)日：2006-11-21

申请号：US10146816

申请日：2002-05-17

申请人： Yasuhiro Goda ,  Ayako Kobayashi ,  Masato Hirobe

发明人： Yasuhiro Goda ,  Ayako Kobayashi ,  Masato Hirobe

IPC分类号： G01N33/53 ,  C07K16/00

CPC分类号： C07K16/26 ,  G01N33/6854

摘要： The present invention provides for a method of selecting an antibody against a target substance to be measured, which comprises selecting the antibody against the target substance by antigen-antibody reaction in the presence of a substance interfering with the antigen-antibody reaction. That is, an antigen and a labeled antigen are reacted with the antibody in the presence of an interfering substance, such as an environment pollutant, and on the basis of the degree of reaction thereof, the antibody against the target substance, which is highly resistant to the interfering substance, is selected. Thereby, even if a test sample is contaminated with a substance interfering with antigen-antibody reaction, the antibody, which is highly resistant to the substance interfering with antigen-antibody reaction, is not influenced by the interfering substance and gives a correct value in the quantification. The present invention further provides for antibodies, which are highly resistant to the substance interfering with the antigen-antibody reaction, and hybridomas producing the antibodies.

摘要翻译： 本发明提供了选择抗被测物质的抗体的方法，该方法包括在干扰抗原 - 抗体反应的物质存在下通过抗原 - 抗体反应选择针对目标物质的抗体。 也就是说，抗原和标记的抗原在抗干扰物质如环境污染物的存在下，与抗体反应，并且基于其抗高度耐药的靶物质的抗体的反应程度 对干扰物质的选择。 因此，即使测试样品被干扰抗原抗体反应的物质污染，对干扰抗原反应的物质高度抗性的抗体也不受干扰物质的影响，并且在 量化。 本发明进一步提供对干扰抗原 - 抗体反应的物质高抗性的抗体和产生抗体的杂交瘤。

公开(公告)号：US20050019833A1

公开(公告)日：2005-01-27

申请号：US10929543

申请日：2004-08-31

申请人： Hideo Ohkawa ,  Shigeru Fujimoto ,  Yasuhiro Goda

发明人： Hideo Ohkawa ,  Shigeru Fujimoto ,  Yasuhiro Goda

IPC分类号： C07K14/47 ,  C07K16/26 ,  C07K16/44 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/13 ,  G01N33/53 ,  A61K39/395

CPC分类号： C07K16/44 ,  C07K14/47 ,  C07K16/26 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2319/00

摘要： The present invention provides a protein capable of binding to an environmental hormone, which protein is supplemented with useful properties for measuring, quantifying and concentrating environmental hormones, such as high sensitivity, low cross-reactivity, unlikelihood of being influenced by interfering substances, and unlikelihood of being influenced by solvents. Specifically, the present invention provides a modified protein prepared by obtaining various genes for various antibodies with environmental hormones, and modifying, by gene recombination technology, various properties of the original antibody, such as affinity, avidity, cross-reactivity, resistance for interfering substance on antigen-antibody reaction, resistance for interfering substance on enzymatic color developing reaction, resistance for solvent and the like.

摘要翻译： 本发明提供能够结合环境激素的蛋白质，该蛋白质补充有用于测量，定量和浓缩环境激素的有用性质，例如高灵敏度，低交叉反应性，受干扰物质影响的不可能性和不可能性 受到溶剂的影响。 具体地说，本发明提供了通过获得具有环境激素的各种抗体的各种基因制备的修饰蛋白质，并且通过基因重组技术修饰了原始抗体的各种性质，例如亲和力，亲合力，交叉反应性，抗干扰物质的抗性 抗原抗体反应，对酶促显色反应的干扰物质的抗性，溶剂性等。

公开(公告)号：US20070072234A1

公开(公告)日：2007-03-29

申请号：US10553305

申请日：2004-04-13

申请人： Norihiro Kobayashi ,  Yasuhiro Goda ,  Masato Hirobe

发明人： Norihiro Kobayashi ,  Yasuhiro Goda ,  Masato Hirobe

IPC分类号： G01N33/53 ,  C07K14/47 ,  C07K16/18

CPC分类号： C07K14/47 ,  G01N33/1826

摘要： The present invention provides a protein having a binding capacity to a plasticizer, which has been conferred with useful properties for measuring, quantifying, or concentrating a plasticizer, such as high sensitivity, low cross-reactivity, high tolerance for interferents, and high tolerance for solvents. Specifically, the present invention provides a modified protein having various properties, such as affinity for antigenic plasticizers, antigen binding capacity, cross-reactivity, tolerance for antigen-antibody reaction interferents, tolerance for enzymatic color developing reaction interferents, and tolerance for solvents, improved by gene recombination technology.

摘要翻译： 本发明提供了具有对增塑剂的结合能力的蛋白质，其具有用于测量，定量或浓缩增塑剂的有用性质，例如高灵敏度，低交叉反应性，对干扰物的高耐受性和高耐受性 溶剂。 具体而言，本发明提供具有各种性质的修饰蛋白质，例如抗原增塑剂的亲和性，抗原结合能力，交叉反应性，抗原抗体反应干扰物的耐受性，酶促显色反应干扰物的耐受性和耐溶剂性，改进 通过基因重组技术。

公开(公告)号：US20060216761A1

公开(公告)日：2006-09-28

申请号：US10516260

申请日：2003-05-30

申请人： Kyoichi Saito ,  Tomofumi Shiraishi ,  Yasuhiro Goda

发明人： Kyoichi Saito ,  Tomofumi Shiraishi ,  Yasuhiro Goda

IPC分类号： G01N33/542 ,  B32B27/04 ,  C12M1/34

CPC分类号： B01J20/26 ,  B01J20/261 ,  B01J20/262 ,  B01J20/264 ,  B01J20/28014 ,  B01J20/28033 ,  B01J20/28038 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3255 ,  B01J20/3274 ,  B01J20/3278 ,  B01J2220/54 ,  B01J2220/58 ,  G01N33/74 ,  Y10T442/2525

摘要： The present invention relates to an isolation material for an endocrine disrupter, which is prepared by immobilizing an anti-endocrine disrupter antibody to a graft-chain-containing carrier made of a polymeric material via covalent binding. According to the present invention, an isolation material for an endocrine disrupter, which is capable of specific and efficient isolation of an endocrine disruptor, and an endocrine disrupter concentration and a clean-up method capable of specific and efficient concentration or clean-up of an endocrine disruptor at a high rate can be afforded.

摘要翻译： 本发明涉及用于内分泌干扰物的分离材料，其通过将抗内分泌干扰物抗体通过共价结合固定在由聚合物材料制成的含接枝链的载体上而制备。 根据本发明，能够特异且有效地分离内分泌干扰物的内分泌干扰物分离材料和内分泌干扰物浓度以及清洁方法能够特异性和有效地浓缩或清除 可以提供高分辨率的内分泌干扰物。

公开(公告)号：US20060110771A1

公开(公告)日：2006-05-25

申请号：US10506063

申请日：2003-02-28

申请人： Masanao Katagiri ,  Shigeru Fujimoto ,  Yasuhiro Goda

发明人： Masanao Katagiri ,  Shigeru Fujimoto ,  Yasuhiro Goda

IPC分类号： G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C07K14/72 ,  C07J5/00

CPC分类号： C07K14/575 ,  C07K14/47 ,  C07K16/26 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/622 ,  G01N33/74 ,  G01N33/743

摘要： The present invention provides a protein capable of binding to a female sex hormone, which protein is supplemented with useful properties for measuring, quantifying and concentrating female sex hormones, such as high sensitivity, low cross-reactivity, unlikelihood of being influenced by interfering substances, and unlikelihood of being influenced by solvents. Specifically, the present invention provides a recombinant protein prepared by obtaining various genes for various antibodies against female sex hormones, and modifying, by gene recombination technology, various properties of the original antibody, such as affinity, avidity, cross-reactivity, resistance for interfering substance on antigen-antibody reaction, resistance for interfering substance on enzymatic color developing reaction, resistance for solvent and the like.

摘要翻译： 本发明提供能够结合雌性激素的蛋白质，该蛋白质补充有用于测量，定量和浓缩女性性激素的有用性质，例如高灵敏度，低交叉反应性，受干扰物质影响的不可靠性， 并且不受溶剂影响。 具体地说，本发明提供了通过获得针对女性性激素的各种抗体的各种基因而制备的重组蛋白质，并且通过基因重组技术修饰原始抗体的各种性质，例如亲和力，亲合力，交叉反应性，抗干扰性 抗原抗体反应的物质，干燥物质对酶显色反应的抵抗性，溶剂性等。

公开(公告)号：US20050244906A1

公开(公告)日：2005-11-03

申请号：US10516823

申请日：2003-06-09

申请人： Akira Kawahara ,  Yasuhiro Goda ,  Naoko Mitsui

发明人： Akira Kawahara ,  Yasuhiro Goda ,  Naoko Mitsui

IPC分类号： C07K16/18 ,  G01N33/68 ,  G01N33/53 ,  C12M1/34 ,  G01N33/537 ,  G01N33/543

CPC分类号： G01N33/68 ,  C07K16/18 ,  G01N2333/4606

摘要： On a surface of a well previously disposed on a plate, a primary antibody that recognizes vitellogenin is solid-phased, in the well a sample obtained from a test body exposed to an environment is injected to react, followed by injecting a secondary antibody that is labeled with an enzyme and recognizes the vitellogenin, further followed by injecting a chromogenic reagent to cause a coloring reaction and by measuring the stained amount, still further followed by calculating an amount of vitellogenin from the stained amount to evaluate an environment based on the amount of vitellogenin.

摘要翻译： 在预先设置在平板上的孔的表面上，识别卵黄发生素的一级抗体是固相的，在井中注射从暴露于环境的测试体获得的样品进行反应，然后注入二级抗体 用酶标记并识别卵黄发生素，然后进一步注射显色试剂以引起着色反应，并通过测量染色量，然后再根据染色量计算出一个量的卵黄发生素，以评价环境的量 卵黄蛋白原

公开(公告)号：US6074451A

公开(公告)日：2000-06-13

申请号：US044961

申请日：1993-04-08

申请人： Yasuhiro Goda

发明人： Yasuhiro Goda

IPC分类号： C02F11/02 ,  B09C1/10 ,  C02F3/34 ,  C05F3/00 ,  C05F11/08 ,  C05F17/00 ,  C12S9/00

CPC分类号： C05F17/0036 ,  B09C1/10 ,  C02F3/34 ,  C05F11/08 ,  C05F3/00 ,  Y02P20/145 ,  Y02W30/43

摘要： A microorganism fertilizer which causes in the soil a phenomenal multiplication of fluorescent Pseudomonas that have a superior effect in preventing the common scab of potato. The microorganism fertilizer is obtained such that medium-temperature and high-temperature Actinomycetes are used as seed bacteria and cultured while being spread/agitated in an organic substance such as livestock manure, etc. in an isolated propagation bed. The thus obtained microorganism fertilizer can be used as a countermeasure against environmental pollution.

摘要翻译： 一种在土壤中引起荧光假单胞菌的显着增殖的微生物肥料，其具有预防马铃薯常见痂病的优异效果。 获得微生物肥料，使得将中等温度和高温放线菌用作种细菌并在分离繁殖床中在诸如家畜粪便等有机物质中扩散/搅拌的同时进行培养。 这样获得的微生物肥料可以用作对抗环境污染的对策。

公开(公告)号：US06251612B1

公开(公告)日：2001-06-26

申请号：US08942915

申请日：1997-10-02

申请人： Yukio Toyoda ,  Masanori Fujita ,  Yasuhiro Goda ,  Ken-ichiro Miyagawa ,  Shigeru Fujimoto

发明人： Yukio Toyoda ,  Masanori Fujita ,  Yasuhiro Goda ,  Ken-ichiro Miyagawa ,  Shigeru Fujimoto

IPC分类号： G01N3353

CPC分类号： C07K16/44 ,  G01N33/5308

摘要： A hybridoma obtainable by fusing a spleen cell or lymphocyte of an animal immunized by a complex of a surfactant compound for synthetic detergent and protein with a myeloma cell, which produces a monoclonal antibody against the compound or its degradation product. A monoclonal antibody against a surfactant compound for synthetic detergent or its degradation product which is produced by the hybridoma, a kit for immunoassay of detergent, its degradation product or a mixture thereof containing as an essential constitutional component the monoclonal antibody, and an immunoassay method, in particular, ELISA of detergent, its degradation product or a mixture thereof in a specimen by reacting the specimen with the monoclonal antibody supported on a carrier and the detergent, its degradation product or the mixture thereof labeled with a labeling agent are also disclosed.

摘要翻译： 通过将由合成洗涤剂和蛋白质的表面活性剂化合物的复合物免疫的动物的脾细胞或淋巴细胞与骨髓瘤细胞融合而获得的杂交瘤，其产生针对该化合物或其降解产物的单克隆抗体。 针对合成洗涤剂或其降解产物的表面活性剂化合物的单克隆抗体，其由杂交瘤产生，用于免疫测定洗涤剂的试剂盒，其降解产物或其含有作为单克隆抗体的必需组成成分的混合物，以及免疫测定法， 特别地，还公开了通过使样品与负载在载体上的单克隆抗体以及用标记试剂标记的洗涤剂，其降解产物或其混合物使样品中的洗涤剂，其降解产物或其混合物的ELISA。