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公开(公告)号:US08765669B2
公开(公告)日:2014-07-01
申请号:US12999654
申请日:2009-06-15
申请人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Naohiro Hayashi , Kazuyuki Ishii , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
发明人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Naohiro Hayashi , Kazuyuki Ishii , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
CPC分类号: C07K14/605 , A61K38/00
摘要: Oligosaccharide chain added GLP-1 peptides are more stable in blood and more active in controlling blood-sugar levels than GLP-1 peptides without added oligosaccharides. Oligosaccharide chain added GLP-1 peptides having GLP-1 activity include at least one or at least two amino acids each substituted with an oligosaccharide chain added amino acid in GLP-1; a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or a GLP-1 analog. Oligosaccharide chain added GLP-1 peptides with at least one amino acid substituted with an oligosaccharide chain added amino acid include an oligosaccharide chain with oligo hyaluronic acid. Oligosaccharide chain added amino acids include oligosaccharide chains attached to amino acids via linkers.
摘要翻译: 添加糖链的GLP-1肽在血液中更稳定,并且在没有添加低聚糖的情况下比GLP-1肽更有效地控制血糖水平。 具有GLP-1活性的添加糖链的GLP-1肽包括GLP-1中由糖链连接的氨基酸取代的至少一个或至少两个氨基酸; 具有GLP-1的氨基酸序列的肽,其具有缺失,取代或添加一个或几个氨基酸; 或GLP-1类似物。 具有至少一个被寡糖链添加的氨基酸取代的氨基酸的添加糖链的GLP-1肽包括具有寡聚透明质酸的寡糖链。 寡糖链添加的氨基酸包括通过接头连接到氨基酸的寡糖链。
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公开(公告)号:US20110195897A1
公开(公告)日:2011-08-11
申请号:US12999654
申请日:2009-06-15
申请人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Naohiro Hayashi , Kazuyuki Ishii , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
发明人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Naohiro Hayashi , Kazuyuki Ishii , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
IPC分类号: A61K38/26 , C07K14/605 , A61P3/10
CPC分类号: C07K14/605 , A61K38/00
摘要: Oligosaccharide chain added GLP-1 peptides are more stable in blood and more active in controlling blood-sugar levels than GLP-1 peptides without added oligosaccharides. Oligosaccharide chain added GLP-1 peptides having GLP-1 activity include at least one or at least two amino acids each substituted with an oligosaccharide chain added amino acid in GLP-1; a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or a GLP-1 analog. Oligosaccharide chain added GLP-1 peptides with at least one amino acid substituted with an oligosaccharide chain added amino acid include an oligosaccharide chain with oligo hyaluronic acid. Oligosaccharide chain added amino acids include oligosaccharide chains attached to amino acids via linkers.
摘要翻译: 添加糖链的GLP-1肽在血液中更稳定,并且在没有添加低聚糖的情况下比GLP-1肽更有效地控制血糖水平。 具有GLP-1活性的添加糖链的GLP-1肽包括GLP-1中由糖链连接的氨基酸取代的至少一个或至少两个氨基酸; 具有GLP-1的氨基酸序列的肽,其具有缺失,取代或添加一个或几个氨基酸; 或GLP-1类似物。 具有至少一个被寡糖链添加的氨基酸取代的氨基酸的添加糖链的GLP-1肽包括具有寡聚透明质酸的寡糖链。 寡糖链添加的氨基酸包括通过接头连接到氨基酸的寡糖链。
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公开(公告)号:US08507429B2
公开(公告)日:2013-08-13
申请号:US13152082
申请日:2011-06-02
申请人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
发明人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
CPC分类号: C07K14/605 , A61K38/00
摘要: The present invention relates to an oligosaccharide chain added GLP-1 peptide that has higher stability in blood than that of GLP-1 and, preferably, exhibits higher activity of controlling blood-sugar levels than that of GLP-1. The present invention relates to an oligosaccharide chain added GLP-1 peptide having GLP-1 activity, wherein at least one amino acid is substituted with an oligosaccharide chain added amino acid, in: (a) GLP-1; (b) a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or (c) a GLP-1 analog.
摘要翻译: 本发明涉及一种与GLP-1相比血液中稳定性更高的糖链附加GLP-1肽,优选表现出比GLP-1更高的控制血糖水平的活性。 本发明涉及:(a)GLP-1;具有GLP-1活性的附加了糖链的GLP-1肽,其中至少一个氨基酸被添加了糖链的氨基酸取代。 (b)具有缺失,取代或添加一个或几个氨基酸的具有GLP-1氨基酸序列的肽; 或(c)GLP-1类似物。
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公开(公告)号:US07985731B2
公开(公告)日:2011-07-26
申请号:US12140866
申请日:2008-06-17
申请人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
发明人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
CPC分类号: C07K14/605 , A61K38/00
摘要: The present invention relates to an oligosaccharide chain added GLP-1 peptide that has higher stability in blood than that of GLP-1 and, preferably, exhibits higher activity of controlling blood-sugar levels than that of GLP-1. The present invention relates to an oligosaccharide chain added GLP-1 peptide having GLP-1 activity, wherein at least one amino acid is substituted with an oligosaccharide chain added amino acid, in: (a) GLP-1; (b) a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or (c) a GLP-1 analog.
摘要翻译: 本发明涉及一种与GLP-1相比血液中稳定性更高的糖链附加GLP-1肽,优选表现出比GLP-1更高的控制血糖水平的活性。 本发明涉及:(a)GLP-1;具有GLP-1活性的附加了糖链的GLP-1肽,其中至少一个氨基酸被添加了糖链的氨基酸取代。 (b)具有缺失,取代或添加一个或几个氨基酸的具有GLP-1氨基酸序列的肽; 或(c)GLP-1类似物。
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公开(公告)号:US20090111739A1
公开(公告)日:2009-04-30
申请号:US12140866
申请日:2008-06-17
申请人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
发明人: Yasuhiro Kajihara , Takashi Tsuji , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Katsunari Tezuka , Hiroaki Asai
CPC分类号: C07K14/605 , A61K38/00
摘要: The present invention relates to an oligosaccharide chain added GLP-1 peptide that has higher stability in blood than that of GLP-1 and, preferably, exhibits higher activity of controlling blood-sugar levels than that of GLP-1. The present invention relates to an oligosaccharide chain added GLP-1 peptide having GLP-1 activity, wherein at least one amino acid is substituted with an oligosaccharide chain added amino acid, in: (a) GLP-1; (b) a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or (c) a GLP-1 analog.
摘要翻译: 本发明涉及一种与GLP-1相比血液中稳定性更高的糖链附加GLP-1肽,优选表现出比GLP-1更高的控制血糖水平的活性。 本发明涉及:(a)GLP-1;具有GLP-1活性的附加了糖链的GLP-1肽,其中至少一个氨基酸被添加了糖链的氨基酸取代。 (b)具有缺失,取代或添加一个或几个氨基酸的具有GLP-1氨基酸序列的肽; 或(c)GLP-1类似物。
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公开(公告)号:US20120047586A9
公开(公告)日:2012-02-23
申请号:US12739480
申请日:2008-10-23
申请人: Takashi Tsuji , Yasuhiro Kajihara , Katsunari Tezuka , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
发明人: Takashi Tsuji , Yasuhiro Kajihara , Katsunari Tezuka , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
CPC分类号: C07K16/00 , A61K38/00 , C07K16/2887 , C07K2317/72 , C07K2317/732 , C07K2319/30 , C07K2319/33
摘要: Disclosed is a polypeptide having an enhanced effector function. Specifically disclosed are: a polypeptide having a modified Fc region; a nucleic acid encoding the polypeptide; a vector carrying the nucleic acid; a host cell or a host organism harboring the vector; a pharmaceutical composition comprising the polypeptide; a method for producing the polypeptide; a method for enhancing the effector function of an antibody; and a method for producing a cell capable of producing an antibody having a high effector function.
摘要翻译: 公开了具有增强的效应子功能的多肽。 具体公开的是:具有修饰的Fc区的多肽; 编码该多肽的核酸; 携带核酸的载体; 携带载体的宿主细胞或宿主生物体; 包含该多肽的药物组合物; 一种生产该多肽的方法; 用于增强抗体的效应子功能的方法; 以及能够产生具有高效应功能的抗体的细胞的制备方法。
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公开(公告)号:US20100306864A1
公开(公告)日:2010-12-02
申请号:US12739480
申请日:2008-10-23
申请人: Takashi Tsuji , Yasuhiro Kajihara , Katsunari Tezuka , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
发明人: Takashi Tsuji , Yasuhiro Kajihara , Katsunari Tezuka , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
CPC分类号: C07K16/00 , A61K38/00 , C07K16/2887 , C07K2317/72 , C07K2317/732 , C07K2319/30 , C07K2319/33
摘要: Disclosed is a polypeptide having an enhanced effector function. Specifically disclosed are: a polypeptide having a modified Fc region; a nucleic acid encoding the polypeptide; a vector carrying the nucleic acid; a host cell or a host organism harboring the vector; a pharmaceutical composition comprising the polypeptide; a method for producing the polypeptide; a method for enhancing the effector function of an antibody; and a method for producing a cell capable of producing an antibody having a high effector function.
摘要翻译: 公开了具有增强的效应子功能的多肽。 具体公开的是:具有修饰的Fc区的多肽; 编码该多肽的核酸; 携带核酸的载体; 携带载体的宿主细胞或宿主生物体; 包含该多肽的药物组合物; 一种生产该多肽的方法; 用于增强抗体的效应子功能的方法; 以及能够产生具有高效应功能的抗体的细胞的制备方法。
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公开(公告)号:US08809258B2
公开(公告)日:2014-08-19
申请号:US12671238
申请日:2008-07-30
申请人: Yasuhiro Kajihara , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
发明人: Yasuhiro Kajihara , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
CPC分类号: C07K1/1133 , C07K1/04 , C07K1/1077 , C07K5/1008 , C07K7/06 , C07K9/00 , C07K14/001 , C07K14/4727
摘要: The present invention provides a method for producing a peptide, characterized in that it comprises converting an —SH group of a peptide comprising an amino acid residue having the —SH group to an —OH group, wherein said method comprises the following steps (a) to (c): (a) allowing an —SH group in a peptide to react with a methylating agent to convert the —SH group to an -SMe group; (b) allowing the -SMe group obtained in the step (a) to react with a cyanizing agent to produce a reaction intermediate; and (c) converting the reaction intermediate obtained in the step (b) to a peptide comprising an amino acid residue having an —OH group under more basic conditions than the conditions in the step (b).
摘要翻译: 本发明提供一种生产肽的方法,其特征在于包括将包含具有-SH基团的氨基酸残基的肽的-SH基转化为-OH基团,其中所述方法包括以下步骤(a) 至(c):(a)使肽中的-SH基团与甲基化剂反应以将-SH基团转化为-SMe基团; (b)使步骤(a)中得到的-SMe基与氰化剂反应生成反应中间体; 和(c)将步骤(b)中获得的反应中间体转化为比在步骤(b)中的条件更碱性的条件下包含具有-OH基团的氨基酸残基的肽。
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公开(公告)号:US20110172392A1
公开(公告)日:2011-07-14
申请号:US12671238
申请日:2008-07-30
申请人: Yasuhiro Kajihara , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
发明人: Yasuhiro Kajihara , Izumi Sakamoto , Yuri Nambu , Kazuhiro Fukae , Hiroaki Asai
IPC分类号: C07K1/113
CPC分类号: C07K1/1133 , C07K1/04 , C07K1/1077 , C07K5/1008 , C07K7/06 , C07K9/00 , C07K14/001 , C07K14/4727
摘要: The present invention provides a method for producing a peptide, characterized in that it comprises converting an —SH group of a peptide comprising an amino acid residue having the —SH group to an —OH group, wherein said method comprises the following steps (a) to (c): (a) allowing an —SH group in a peptide to react with a methylating agent to convert the —SH group to an —SMe group; (b) allowing the —SMe group obtained in the step (a) to react with a cyanizing agent to produce a reaction intermediate; and (c) converting the reaction intermediate obtained in the step (b) to a peptide comprising an amino acid residue having an —OH group under more basic conditions than the conditions in the step (b).
摘要翻译: 本发明提供一种生产肽的方法,其特征在于包括将包含具有-SH基团的氨基酸残基的肽的-SH基转化为-OH基团,其中所述方法包括以下步骤(a) 至(c):(a)使肽中的-SH基团与甲基化剂反应以将-SH基团转化为-SMe基团; (b)使步骤(a)中得到的-SMe基与氰化剂反应生成反应中间体; 和(c)将步骤(b)中获得的反应中间体转化为比在步骤(b)中的条件更碱性的条件下包含具有-OH基团的氨基酸残基的肽。
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公开(公告)号:US20120264684A1
公开(公告)日:2012-10-18
申请号:US13502723
申请日:2010-10-25
申请人: Yasuhiro Kajihara , Takashi Tsuji , Yuri Nambu , Kazuyuki Ishii , Kenta Yoshida , Katsunari Tezuka
发明人: Yasuhiro Kajihara , Takashi Tsuji , Yuri Nambu , Kazuyuki Ishii , Kenta Yoshida , Katsunari Tezuka
IPC分类号: C07K14/605 , A61P3/10 , A61K38/26
CPC分类号: C07K14/605 , A61K38/00
摘要: Disclosed is a glucagon-like peptide-1 (GLP-1) analogue which is obtained by ameliorating a highly antigenic GLP-1 analogue so that the GLP-1 analogue has reduced antigenicity without being lowered in the blood glucose suppressing activity. Specifically disclosed is a glycosylated form of an antigenic GLP-1 analogue, which has GLP-1 activity and is obtained by substituting at least one amino acid of an antigenic GLP-1 analogue with a glycosylated amino acid.
摘要翻译: 公开了通过改善高度抗原性GLP-1类似物而获得的胰高血糖素样肽-1(GLP-1)类似物,使得GLP-1类似物具有降低的抗原性而不降低血糖抑制活性。 具体公开的是具有GLP-1活性的抗原性GLP-1类似物的糖基化形式,其通过用糖基化氨基酸取代抗原性GLP-1类似物的至少一个氨基酸获得。
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