公开(公告)号：US5834212A

公开(公告)日：1998-11-10

申请号：US417847

申请日：1995-04-06

申请人： Yasunori Okada ,  Masayoshi Shinmei ,  Taro Hayakawa ,  Kazushi Iwata ,  Yumi Korin ,  Shuji Kodama ,  Shinichi Yoshida

发明人： Yasunori Okada ,  Masayoshi Shinmei ,  Taro Hayakawa ,  Kazushi Iwata ,  Yumi Korin ,  Shuji Kodama ,  Shinichi Yoshida

IPC分类号： C12N5/10 ,  C07K16/00 ,  C07K16/40 ,  C07K19/00 ,  C12N5/20 ,  C12N9/64 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/564 ,  G01N33/573 ,  G01N33/577 ,  G01N33/543

CPC分类号： C12N9/6491 ,  C07K16/40

摘要： Anti-human stromelysin monoclonal antibodies reactive with latent and active forms of stromelysin without discrimination between the two, each being immunoreactive with only one of the antigenic determinants of human stromelysin, are provided. The use of a combination of two such monoclonal antibodies which specifically react with different antigenic determinants of human stromelysin renders it possible to accurately determine the amount of human stromelysin in human body fluids, and thus to carry out the diagnosis of rheumatoid arthritis. There are thus provided said monoclonal antibodies per se, a sandwich enzyme immunoassay for the determination of the amount of human stromelysin in human body fluid samples using a combination of two such monoclonal antibodies, and a method for the diagnosis of rheumatoid arthritis using said immunoassay.

摘要翻译： 与人类基质溶素的一个抗原决定簇之间的免疫反应性提供了两者之间的区别两者的潜在和活性形式的基质溶素的抗人基质溶素蛋白单克隆抗体。 使用与人类溶基质蛋白酶的不同抗原决定簇特异性反应的两种这样的单克隆抗体的组合使得可以准确地确定人体液中的人类基质溶素的量，从而进行类风湿性关节炎的诊断。 因此，提供了所述单克隆抗体本身，使用两种这样的单克隆抗体的组合来测定人体液样品中人基质溶素的量的夹心酶免疫测定法和使用所述免疫测定法诊断类风湿性关节炎的方法。