公开(公告)号：US09492842B2

公开(公告)日：2016-11-15

申请号：US14128089

申请日：2012-06-22

申请人： Yasuo Yoshimoto ,  Kentaro Kamimae ,  Yuki Tanabe ,  Taku Oguni ,  Takashi Inoue ,  Tsutomu Mochizuki ,  Makoto Hirama ,  Teruo Fujii ,  Hiroshi Kimura ,  Hideto Tozawa

发明人： Yasuo Yoshimoto ,  Kentaro Kamimae ,  Yuki Tanabe ,  Taku Oguni ,  Takashi Inoue ,  Tsutomu Mochizuki ,  Makoto Hirama ,  Teruo Fujii ,  Hiroshi Kimura ,  Hideto Tozawa

IPC分类号： B05D1/00 ,  C12M1/12 ,  C12N5/00 ,  C12M1/00

CPC分类号： B05D1/60 ,  C12M23/20 ,  C12M25/02 ,  C12N5/0068 ,  C12N2533/30

摘要： Provided is: a cell culture membrane, which is free from materials derived from living organisms, can easily be industrially mass-produced, exhibits superior long-term storage properties and chemical resistance, has excellent cell adhesion properties and long-term culture properties and is capable of replicating a cell adhesion morphology that is similar to that of collagen derived from living organisms and being used for conventional cell cultivation. Also provided are a cell culture substrate, and a method for manufacturing the cell culture substrate. In the present invention, as a cell adhesion layer, a polymer membrane represented by formula (I) is formed on the base of a cell culture substrate so as to have a membrane thickness equal to or greater than 0.2 μm (in the formula, R1 and R2 represent a —(CH2)n—NH2 moiety (n is an integer of 1-10 inclusive.) or H, with at least one of R1 and R2 being a —(CH2)n—NH2 moiety. Moreover, l and m are positive integers expressing polymerization degree).

公开(公告)号：US20140212974A1

公开(公告)日：2014-07-31

申请号：US14128089

申请日：2012-06-22

申请人： Yasuo Yoshimoto ,  Kentaro Kamimae ,  Yuki Tanabe ,  Taku Oguni ,  Takashi Inoue ,  Tsutomu Mochizuki ,  Makoto Hirama ,  Teruo Fujii ,  Hiroshi Kimura ,  Hideto Tozawa

发明人： Yasuo Yoshimoto ,  Kentaro Kamimae ,  Yuki Tanabe ,  Taku Oguni ,  Takashi Inoue ,  Tsutomu Mochizuki ,  Makoto Hirama ,  Teruo Fujii ,  Hiroshi Kimura ,  Hideto Tozawa

IPC分类号： C12N5/00 ,  B05D1/00

CPC分类号： B05D1/60 ,  C12M23/20 ,  C12M25/02 ,  C12N5/0068 ,  C12N2533/30

摘要： Provided is: a cell culture membrane, which is free from materials derived from living organisms, can easily be industrially mass-produced, exhibits superior long-term storage properties and chemical resistance, has excellent cell adhesion properties and long-term culture properties and is capable of replicating a cell adhesion morphology that is similar to that of collagen derived from living organisms and being used for conventional cell cultivation. Also provided are a cell culture substrate, and a method for manufacturing the cell culture substrate. In the present invention, as a cell adhesion layer, a polymer membrane represented by formula (I) is formed on the base of a cell culture substrate so as to have a membrane thickness equal to or greater than 0.2 μm (in the formula, R1 and R2 represent a —(CH2)n—NH2 moiety (n is an integer of 1-10 inclusive.) or H, with at least one of R1 and R2 being a —(CH2)n—NH2 moiety. Moreover, l and m are positive integers expressing polymerization degree).

摘要翻译： 提供：不含活体的物质的细胞培养膜可以容易地在工业上批量生产，具有优异的长期保存性和耐化学性，具有优异的细胞粘附性和长期培养性，并且是 能够复制与来自活生物体的胶原相似的细胞粘附形态，并用于常规细胞培养。 还提供了细胞培养基质和细胞培养基质的制造方法。 在本发明中，作为细胞粘附层，在细胞培养基材的基材上形成由式（I）表示的聚合物膜，以使膜厚等于或大于0.2μm（式中，R1 并且R 2表示 - （CH 2）n -NH 2部分（n是包括1-10的整数）或H，其中R 1和R 2中的至少一个是 - （CH 2）n -NH 2部分，此外， m是表示聚合度的正整数）。