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    CELL CULTURE MEMBRANE, CELL CULTURE SUBSTRATE, AND METHOD FOR MANUFACTURING CELL CULTURE SUBSTRATE
    1.
    发明申请
    CELL CULTURE MEMBRANE, CELL CULTURE SUBSTRATE, AND METHOD FOR MANUFACTURING CELL CULTURE SUBSTRATE 有权
    细胞培养膜，细胞培养基质和制备细胞培养基质的方法

    公开(公告)号：US20140212974A1

    公开(公告)日：2014-07-31

    申请号：US14128089

    申请日：2012-06-22

    申请人： Yasuo Yoshimoto ,  Kentaro Kamimae ,  Yuki Tanabe ,  Taku Oguni ,  Takashi Inoue ,  Tsutomu Mochizuki ,  Makoto Hirama ,  Teruo Fujii ,  Hiroshi Kimura ,  Hideto Tozawa

    发明人： Yasuo Yoshimoto ,  Kentaro Kamimae ,  Yuki Tanabe ,  Taku Oguni ,  Takashi Inoue ,  Tsutomu Mochizuki ,  Makoto Hirama ,  Teruo Fujii ,  Hiroshi Kimura ,  Hideto Tozawa

    IPC分类号： C12N5/00 ,  B05D1/00

    CPC分类号： B05D1/60 ,  C12M23/20 ,  C12M25/02 ,  C12N5/0068 ,  C12N2533/30

    摘要： Provided is: a cell culture membrane, which is free from materials derived from living organisms, can easily be industrially mass-produced, exhibits superior long-term storage properties and chemical resistance, has excellent cell adhesion properties and long-term culture properties and is capable of replicating a cell adhesion morphology that is similar to that of collagen derived from living organisms and being used for conventional cell cultivation. Also provided are a cell culture substrate, and a method for manufacturing the cell culture substrate. In the present invention, as a cell adhesion layer, a polymer membrane represented by formula (I) is formed on the base of a cell culture substrate so as to have a membrane thickness equal to or greater than 0.2 μm (in the formula, R1 and R2 represent a —(CH2)n—NH2 moiety (n is an integer of 1-10 inclusive.) or H, with at least one of R1 and R2 being a —(CH2)n—NH2 moiety. Moreover, l and m are positive integers expressing polymerization degree).

    摘要翻译： 提供：不含活体的物质的细胞培养膜可以容易地在工业上批量生产，具有优异的长期保存性和耐化学性，具有优异的细胞粘附性和长期培养性，并且是 能够复制与来自活生物体的胶原相似的细胞粘附形态，并用于常规细胞培养。 还提供了细胞培养基质和细胞培养基质的制造方法。 在本发明中，作为细胞粘附层，在细胞培养基材的基材上形成由式（I）表示的聚合物膜，以使膜厚等于或大于0.2μm（式中，R1 并且R 2表示 - （CH 2）n -NH 2部分（n是包括1-10的整数）或H，其中R 1和R 2中的至少一个是 - （CH 2）n -NH 2部分，此外， m是表示聚合度的正整数）。

    Buffer capacity change monitoring method and apparatus
    3.
    发明授权
    Buffer capacity change monitoring method and apparatus 失效
    缓冲容量变化监测方法及装置

    公开(公告)号：US5862409A

    公开(公告)日：1999-01-19

    申请号：US580021

    申请日：1995-12-20

    申请人： Yasuo Yoshimoto ,  Hiroki Sumida

    发明人： Yasuo Yoshimoto ,  Hiroki Sumida

    IPC分类号： G06F12/02 ,  G06F5/10 ,  G06F11/32 ,  G06F11/34

    CPC分类号： G06F5/10 ,  G06F11/3452 ,  G06F11/324 ,  G06F2201/81

    摘要： During monitoring of a buffer configured so that expansion of an extent c of a buffer capacity takes place when the size of an unused region is reduced to a required expansion threshold value b, execution of a required calculation using an expansion threshold value b and buffer capacity extent c and c' (where c' is an initial value) for each point of time occurs. The reference values s1 and s2 corresponding to buffer capacities c and c' for each time point are determined and those reference values are used as the basis for screen display of expansion changes to the extent of the buffer capacity, thereby ensuring that a content of a display occurs corresponding to expansion for the extent of a buffer capacity. In addition, the use of the calculation formula for reference value s1 or s2 can be selected, while it is also possible to use a reference value (a fixed value ) which has been defined by the user.

    摘要翻译： 在监视被配置为使得当未使用区域的尺寸减小到所需的扩展阈值b时发生缓冲器容量的扩展c的缓冲器的监视中，使用扩展阈值b和缓冲器容量执行所需的计算 发生每个时间点的范围c和c'（其中c'是初始值）。 确定对应于每个时间点的缓冲器容量c和c'的参考值s1和s2，并且将这些参考值用作作为缓冲器容量程度的扩展改变的屏幕显示的基础，由此确保 显示器对应于缓冲容量的扩展。 此外，可以选择使用参考值s1或s2的计算公式，同时也可以使用由用户定义的参考值（固定值）。