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    Nucleic acid concatenation
    1.
    发明申请
    Nucleic acid concatenation 审中-公开
    核酸连接

    公开(公告)号：US20080124707A1

    公开(公告)日：2008-05-29

    申请号：US11449872

    申请日：2006-06-09

    申请人： Yijun Ruan ,  Patrick Ng ,  Melissa Jane Fullwood ,  Yen Ling Lee

    发明人： Yijun Ruan ,  Patrick Ng ,  Melissa Jane Fullwood ,  Yen Ling Lee

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6804 ,  C12Q1/6809 ,  C12Q2525/151 ,  C12Q2525/131 ,  C12Q2539/103

    摘要： The present invention provides a method of manipulating nucleic acids. In particular, of length-controlled concatenating of nucleotide fragments, the method comprising: (a) providing at least two nucleotide fragments, wherein each fragment has one ligatable end and one non-ligatable end; and (b) allowing the two fragments to ligate at the ligatable ends to form an oligonucleotide comprising at least two concatenated nucleotide fragments. The present invention also provides an isolated oligonucleotide comprising at least two nucleotide fragments, wherein each fragment has at least one ligatable end and and one non-ligatable end, and the fragments are ligated at the ligatable ends to form the oligonucleotide.

    摘要翻译： 本发明提供了一种操纵核酸的方法。 特别地，长度控制的核苷酸片段连接，所述方法包括：（a）提供至少两个核苷酸片段，其中每个片段具有一个可连接末端和一个不可连接末端; 和（b）允许两个片段在可连接的末端连接以形成包含至少两个连接的核苷酸片段的寡核苷酸。 本发明还提供了包含至少两个核苷酸片段的分离的寡核苷酸，其中每个片段具有至少一个可连接的末端和一个不可连接的末端，并且将片段连接在可连接的末端以形成寡核苷酸。

    Method for gene identification signature (GIS) analysis
    2.
    发明授权
    Method for gene identification signature (GIS) analysis 有权
    基因鉴别特征（GIS）分析方法

    公开(公告)号：US07553947B2

    公开(公告)日：2009-06-30

    申请号：US11610118

    申请日：2006-12-13

    申请人： Patrick Ng ,  Chialin Wei ,  Yijun Ruan

    发明人： Patrick Ng ,  Chialin Wei ,  Yijun Ruan

    IPC分类号： C12N15/11 ,  C12N15/63 ,  C07H21/04

    CPC分类号： C12Q1/6809 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6855 ,  C12Q2525/191 ,  C12Q2525/131 ,  C12Q2521/313

    摘要： A method of identifying at least a nucleic acid molecule fragment to which a protein of interest binds, comprising: (i) preparing at least one nucleic acid molecule fragment to which a protein binds; (ii) isolating the 5′ terminus and the 3′ terminus of the nucleic acid fragment(s) and linking the 5′ terminus and 3′ terminus to create the at least one ditag; (iii) sequencing the ditag; and (iv) mapping the ditag sequence(s) to the genome.

    摘要翻译： 鉴定至少一个目标蛋白质所结合的核酸分子片段的方法，包括：（i）制备蛋白质结合至少一个核酸分子片段; （ii）分离核酸片段的5'末端和3'末端并连接5'末端和3'末端以产生至少一个ditag; （iii）测序ditag; 和（iv）将分离序列映射到基因组。

    Method for Gene Identification Signature (GIS) Analysis
    3.
    发明申请
    Method for Gene Identification Signature (GIS) Analysis 有权
    基因鉴别特征（GIS）分析方法

    公开(公告)号：US20070161024A1

    公开(公告)日：2007-07-12

    申请号：US11610118

    申请日：2006-12-13

    申请人： Patrick Ng ,  Chialin Wei ,  Yijun Ruan

    发明人： Patrick Ng ,  Chialin Wei ,  Yijun Ruan

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12Q1/6809 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6855 ,  C12Q2525/191 ,  C12Q2525/131 ,  C12Q2521/313

    摘要： A method of identifying at least a nucleic acid molecule fragment to which a protein of interest binds, comprising: (i) preparing at least one nucleic acid molecule fragment to which a protein binds; (ii) isolating the 5′ terminus and the 3′ terminus of the nucleic acid fragment(s) and linking the 5′ terminus and 3′ terminus to create the at least one ditag; (iii) sequencing the ditag; and (iv) mapping the ditag sequence(s) to the genome.

    摘要翻译： 鉴定至少一个目标蛋白质所结合的核酸分子片段的方法，包括：（i）制备蛋白质结合至少一个核酸分子片段; （ii）分离核酸片段的5'末端和3'末端并连接5'末端和3'末端以产生至少一个ditag; （iii）测序ditag; 和（iv）将分离序列映射到基因组。

    Method for gene identification signature (GIS) analysis
    6.
    发明申请
    Method for gene identification signature (GIS) analysis 审中-公开
    基因鉴别特征（GIS）分析方法

    公开(公告)号：US20050255501A1

    公开(公告)日：2005-11-17

    申请号：US11045468

    申请日：2005-01-31

    申请人： Patrick Ng ,  Chialin Wei ,  Yijun Ruan

    发明人： Patrick Ng ,  Chialin Wei ,  Yijun Ruan

    IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68

    CPC分类号： C12Q1/6809 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6855 ,  C12Q2525/191 ,  C12Q2525/131 ,  C12Q2521/313

    摘要： A method of identifying at least a nucleic acid molecule fragment to which a protein of interest binds, comprising: (i) preparing at least one nucleic acid molecule fragment to which a protein binds; (ii) isolating the 5′ terminus and the 3′ terminus of the nucleic acid fragment(s) and linking the 5′ terminus and 3′ terminus to create the at least one ditag; (iii) sequencing the ditag; and (iv) mapping the ditag sequence(s) to the genome.

    摘要翻译： 鉴定至少一个目标蛋白质所结合的核酸分子片段的方法，包括：（i）制备蛋白质结合至少一个核酸分子片段; （ii）分离核酸片段的5'末端和3'末端并连接5'末端和3'末端以产生至少一个ditag; （iii）测序ditag; 和（iv）将分离序列映射到基因组。

    VERIFICATION AND PROTECTION OF GENUINE SOFTWARE INSTALLATION USING HARDWARE SUPER KEY
    8.
    发明申请
    VERIFICATION AND PROTECTION OF GENUINE SOFTWARE INSTALLATION USING HARDWARE SUPER KEY 有权
    使用硬件超级钥匙验证和保护通用软件安装

    公开(公告)号：US20120079277A1

    公开(公告)日：2012-03-29

    申请号：US12890304

    申请日：2010-09-24

    申请人： Patrick Ng

    发明人： Patrick Ng

    IPC分类号： H04L9/32 ,  H04L9/08

    CPC分类号： H04L9/0822 ,  G06F8/61 ,  G06F21/10 ,  H04L63/18 ,  H04L2209/12 ,  H04L2463/061 ,  H04L2463/062

    摘要： A device, system, and method are disclosed. In one embodiment the device receives a user key from a user application. The device then creates a management engine key by applying a management engine key creation algorithm to the user key. Then the device sends the management engine key to a remote server. Later, the device retrieves a server key from the remote server. The device next performs a hash combination of the user key, the management engine key, and the server key to create a super key. Once the super key has been created, the device authenticates the super key, and if the super key is valid, the device then sends a management engine certification to the user application.

    摘要翻译： 公开了一种设备，系统和方法。 在一个实施例中，设备从用户应用接收用户密钥。 然后，设备通过将管理引擎密钥创建算法应用于用户密钥来创建管理引擎密钥。 然后设备将管理引擎密钥发送到远程服务器。 之后，设备从远程服务器检索服务器密钥。 该设备接下来执行用户密钥，管理引擎密钥和服务器密钥的哈希组合以创建超级密钥。 一旦创建了超级密钥，设备将对超级密钥进行认证，如果超级密钥有效，则设备会向用户应用发送管理引擎认证。

    Anti-growth factor receptor avidin fusion proteins as universal vectors for drug delivery
    9.
    发明申请
    Anti-growth factor receptor avidin fusion proteins as universal vectors for drug delivery 审中-公开
    抗生长因子受体亲和素融合蛋白作为药物递送的通用载体

    公开(公告)号：US20050085419A1

    公开(公告)日：2005-04-21

    申请号：US10858729

    申请日：2004-06-02

    申请人： Sherie Morrison ,  William Pardridge ,  Manuel Penichet ,  Josefina Coloma ,  Shin Seung-Uon ,  Patrick Ng

    发明人： Sherie Morrison ,  William Pardridge ,  Manuel Penichet ,  Josefina Coloma ,  Shin Seung-Uon ,  Patrick Ng

    IPC分类号： C07K14/465 ,  C07K16/28 ,  C12N15/87 ,  A61K38/17 ,  C07K14/72

    CPC分类号： C12N15/87 ,  A61K2039/505 ,  C07K14/465 ,  C07K16/2863 ,  C07K16/2881 ,  C07K2317/77 ,  C07K2319/00

    摘要： A fusion protein for delivery of a wide variety of agents to a cell via antibody-receptor-mediated endocytosis comprises a first segment and a second segment: the first segment comprising a variable region of an antibody that recognizes an antigen on the surface of a cell that after binding to the variable region of the antibody undergoes antibody-receptor-mediated endocytosis, and, optionally, further comprises at least one domain of a constant region of an antibody; and the second segment comprising a protein domain selected from the group consisting of avidin, an avidin mutein, a chemically modified avidin derivative, streptavidin, a streptavidin mutein, and a chemically modified streptavidin derivative. Typically, the antigen is a protein. Typically, the protein antigen on the surface of the cell is a receptor such as a transferrin receptor-or an insulin receptor. The invention also includes an antibody construct incorporating the fusion protein that is either a heavy chain or a light chain together with a complementary light chain or heavy chain to form an intact antibody molecule. The invention further includes targeting methods and screening methods.

    摘要翻译： 用于通过抗体 - 受体介导的内吞将多种试剂递送至细胞的融合蛋白包括第一区段和第二区段：第一区段包含识别细胞表面上的抗原的抗体的可变区 在与抗体的可变区结合后经历抗体受体介导的内吞作用，并且任选地还包含抗体恒定区的至少一个结构域; 并且第二片段包含选自抗生物素蛋白，抗生物素蛋白突变蛋白，化学修饰的亲和素衍生物，链霉亲和素，链霉抗生物素蛋白突变蛋白和化学修饰的链霉亲和素衍生物的蛋白质结构域。 通常，抗原是蛋白质。 通常，细胞表面上的蛋白质抗原是受体，例如转铁蛋白受体或胰岛素受体。 本发明还包括结合融合蛋白的抗体构建体，其是重链或轻链以及互补轻链或重链以形成完整的抗体分子。 本发明还包括靶向方法和筛选方法。