公开(公告)号：US20060062770A1

公开(公告)日：2006-03-23

申请号：US11203804

申请日：2005-08-15

申请人： Ying Zheng ,  Xiaobing Du ,  Wei Wang ,  Marylene Boucher ,  Satish Parimoo ,  Kurt Stenn

发明人： Ying Zheng ,  Xiaobing Du ,  Wei Wang ,  Marylene Boucher ,  Satish Parimoo ,  Kurt Stenn

IPC分类号： A61K35/36

CPC分类号： A61K35/36 ,  A61K8/02 ,  A61K8/985 ,  A61K35/12 ,  A61Q7/00 ,  C12N5/0627

摘要： A method assay for rapidly and reproducibly generating hair follicles from dissociated epithelial and mesenchymal cells is disclosed. The method serves both as a tool for measuring the trichogenic (i.e., hair growth-inducing) property of cells and for studying the mechanisms dissociated cells employ to assemble an organ. In a method of this application dissociated cells, isolated from newborn mouse skin, are injected into adult mouse truncal skin, hair follicles develop. This process involves the aggregation of epithelial cells to form clusters which are sculpted by apoptosis to generate “infundibular cysts”. From the “infundibular cysts” hair germs form followed by follicular buds and then pegs which grow asymmetrically to differentiate into cycling mature pilosebaceous structures. Using various techniques, exposure of the “infundibular cysts” by puncturing, piercing, or scratching the skin and, in an approach, covering the exposed cysts with a wound dressing material produced egressing hair follicles.

摘要翻译： 公开了一种用于从解离的上皮和间充质细胞中快速和可重复地产生毛囊的方法测定法。 该方法既用于测量细胞的致毛体（即毛发生长诱导）性质和用于研究解离的细胞用于组装器官的机制。 在这种方法中，从新生小鼠皮肤分离的解离的细胞被注射到成年小鼠的截肢皮肤中，形成毛囊。 该过程涉及上皮细胞的聚集以形成通过凋亡而雕刻以产生“漏斗状囊肿”的簇。 从“漏斗状囊肿”毛发细胞形式，然后是滤泡芽，然后是不对称生长的分离成循环成熟的皮脂状结构的栓。 使用各种技术，通过穿刺，刺穿或刮伤皮肤暴露“漏斗状囊肿”，并且在使用产生流出毛囊的伤口敷料材料覆盖暴露的囊肿的方法中。

公开(公告)号：US20120095445A1

公开(公告)日：2012-04-19

申请号：US13378732

申请日：2010-06-16

申请人： Ying Zheng ,  Marylene Boucher ,  Ying Homan ,  Charles Hollow ,  Polina Mamontov ,  Kurt Stenn

发明人： Ying Zheng ,  Marylene Boucher ,  Ying Homan ,  Charles Hollow ,  Polina Mamontov ,  Kurt Stenn

IPC分类号： A61K35/12 ,  A61P17/14 ,  A61M37/00 ,  C12N5/071

CPC分类号： C07D409/12 ,  A61K31/381 ,  A61K31/4436

摘要： Methods and compositions for increasing trichogenicity of cells in culture are provided. One embodiment provides culturing dissociated mammalian dermal cells in vitro in the presence of an effective amount of one or more sonic hedgehog (Shh) pathway agonists to increase the trichogenicity of the dissociated mammalian dermal cells compared to untreated dissociated mammalian dermal cells. The cell culture optionally includes epidermal cells. Preferred Shh agonists include, but are not limited to CUR-0236715 and CUR-0201365 available from Curis, Inc. Trichogenicity is measured using the Aderans Hair Patch Assay™. The cultured dermal cells can be maintained in culture in the presence of the one or more Shh agonists for at least 1 to 7 or more days prior to harvest. The treated, cultured dermal cells can be used to treat hair loss in a mammalian subject, preferably a human, by implanting them in an effective amount to induce hair follicle formation.

摘要翻译： 提供了用于增加培养物中细胞的致毛性的方法和组合物。 一个实施方案在有效量的一种或多种声刺猬（Shh）途径激动剂的存在下提供培养解离的哺乳动物真皮细胞，以增加与未处理的解离的哺乳动物真皮细胞相比，解离的哺乳动物真皮细胞的致毛性。 细胞培养物任选地包括表皮细胞。 优选的Shh激动剂包括但不限于可从Curis，Inc。获得的CUR-0236715和CUR-0201365。使用Aderans Hair Patch Assay TM测量Trichoigenicity。 培养的真皮细胞可以在收获前至少1至7天或更多天在一种或多种Shh激动剂存在下保持培养。 经处理的培养的真皮细胞可用于通过将它们植入有效量以诱导毛囊形成来治疗哺乳动物受试者，优选人类的脱发。