公开(公告)号：US08835163B2

公开(公告)日：2014-09-16

申请号：US12445505

申请日：2007-10-18

申请人： Yong Zhao ,  Theodore Mazzone

发明人： Yong Zhao ,  Theodore Mazzone

IPC分类号： C12N5/10 ,  A61K35/14 ,  C12N5/071 ,  A61K35/54 ,  C12N5/074 ,  C12N5/078 ,  A61K35/39 ,  A61K35/12

CPC分类号： C12N5/0607 ,  A61K35/17 ,  A61K35/39 ,  A61K35/545 ,  A61K2035/122 ,  C12N5/0634 ,  C12N5/0676 ,  C12N2500/34 ,  C12N2501/22 ,  C12N2501/335 ,  C12N2506/03

摘要： The present invention is related generally to embryonic-like stem cells isolated from adult human peripheral blood, designated herein as peripheral blood-stem cells (PB-SC), which display the characteristics of embryonic stem cells and hematopoietic cells. These cells have the capability of proliferation and are able to differentiate to other types of cells. These cells are, therefore, suitable for use in stem cell-based therapies, particularly autologous stem cell therapies, for the treatment of various diseases such as neurodegenerative diseases, autoimmune diseases, diabetes, spinal cord damage, multiple sclerosis, cardiovascular disease, stroke and birth defects.

摘要翻译： 本发明一般涉及从成人外周血分离的胚胎样干细胞，本文命名为外周血干细胞（PB-SC），其显示胚胎干细胞和造血细胞的特征。 这些细胞具有增殖能力，能够区分到其他类型的细胞。 因此，这些细胞适合用于基于干细胞的治疗，特别是自体干细胞治疗，用于治疗各种疾病，例如神经退行性疾病，自身免疫性疾病，糖尿病，脊髓损伤，多发性硬化，心血管疾病，中风和 出生缺陷。

公开(公告)号：US20070059824A1

公开(公告)日：2007-03-15

申请号：US11519307

申请日：2006-09-12

申请人： Yong Zhao ,  Theodore Mazzone

发明人： Yong Zhao ,  Theodore Mazzone

IPC分类号： C12N5/08

CPC分类号： C12N5/0647 ,  C12N5/0607 ,  C12N5/0676 ,  C12N2500/34 ,  C12N2501/052 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2506/03 ,  C12N2506/11

摘要： The present invention relates to a purified population of fibroblast-like macrophage (f-macrophage, f-MΦ) and methods using the same. The f-MΦ can be expanded in vitro and differentiated into several lineages, including insulin-expressing cells, endothelial cells, and neuronal cells. The f-MΦ described herein have been generated from human umbilical cord blood (CB f-MΦ) and have characteristics similar to f-MΦ derived from peripheral blood. Thrombopoietin (TPO), at low dosage, significantly stimulates the proliferation of CB f-MΦ, wherein the TPO-expanded CB f-MΦ retain their pluripotent differentiation potential.

摘要翻译： 本发明涉及纯化的成纤维细胞样巨噬细胞群（f-巨噬细胞，f-MPhi）和使用其的方法。 f-MPhi可以在体外扩增并分化成几个谱系，包括表达胰岛素的细胞，内皮细胞和神经元细胞。 本文描述的f-MPhi已经由人脐带血（CB f-MPhi）产生并且具有与来自外周血的f-MPhi相似的特征。 血小板生成素（TPO）以低剂量显着刺激CB f-MPhi的增殖，其中TPO扩张的CB f-MPhi保留其多能分化潜能。

公开(公告)号：US09388382B2

公开(公告)日：2016-07-12

申请号：US12099054

申请日：2008-04-07

申请人： Yong Zhao ,  Theodore Mazzone

发明人： Yong Zhao ,  Theodore Mazzone

IPC分类号： C12N5/00 ,  C12N5/074 ,  A61K35/12

CPC分类号： C12N5/0607 ,  A61K2035/122

摘要： The present invention is related generally to embryonic-like stem cells isolated from human umbilical cord blood, designated herein as cord blood-stem cells (CB-SC's), which display the characteristics of embryonic stem cells and hematopoietic cells. These cells have the capability of proliferation and are able to differentiate to multiple types of cells. In addition, the CB-SC display low immunogenicity and immune regulation. These cells are, therefore, suitable for use in stem cell-based therapies for the treatment of diseases such as Parkinson's disease, diabetes, spinal cord damage, multiple sclerosis, cardiovascular disease, stroke and birth defects, and for preventing, treating and/or reducing an autoimmune disease in a mammalian subject.

摘要翻译： 本发明一般涉及从人脐带血分离的胚胎样干细胞，本文称为脐带血干细胞（CB-SC），其显示胚胎干细胞和造血细胞的特征。 这些细胞具有增殖能力，能够分化成多种类型的细胞。 此外，CB-SC显示低免疫原性和免疫调节。 因此，这些细胞适合用于治疗诸如帕金森病，糖尿病，脊髓损伤，多发性硬化，心血管疾病，中风和出生缺陷的疾病的基于干细胞的疗法，以及用于预防，治疗和/ 减少哺乳动物受试者的自身免疫性疾病。

公开(公告)号：US20100129440A1

公开(公告)日：2010-05-27

申请号：US12445505

申请日：2007-10-18

申请人： Yong Zhao ,  Theodore Mazzone

发明人： Yong Zhao ,  Theodore Mazzone

IPC分类号： A61K9/48 ,  C12N5/02 ,  C12N5/071 ,  A61K35/12

CPC分类号： C12N5/0607 ,  A61K35/17 ,  A61K35/39 ,  A61K35/545 ,  A61K2035/122 ,  C12N5/0634 ,  C12N5/0676 ,  C12N2500/34 ,  C12N2501/22 ,  C12N2501/335 ,  C12N2506/03

摘要： The present invention is related generally to embryonic-like stem cells isolated from adult human peripheral blood, designated herein as peripheral blood-stem cells (PB-SC), which display the characteristics of embryonic stem cells and hematopoietic cells. These cells have the capability of proliferation and are able to differentiate to other types of cells. These cells are, therefore, suitable for use in stem cell-based therapies, particularly autologous stem cell therapies, for the treatment of various diseases such as neurodegenerative diseases, autoimmune diseases, diabetes, spinal cord damage, multiple sclerosis, cardiovascular disease, stroke and birth defects.

摘要翻译： 本发明一般涉及从成人外周血分离的胚胎样干细胞，本文命名为外周血干细胞（PB-SC），其显示胚胎干细胞和造血细胞的特征。 这些细胞具有增殖能力，能够区分到其他类型的细胞。 因此，这些细胞适合用于基于干细胞的治疗，特别是自体干细胞治疗，用于治疗各种疾病，例如神经退行性疾病，自身免疫性疾病，糖尿病，脊髓损伤，多发性硬化，心血管疾病，中风和 出生缺陷。