公开(公告)号：US07041635B2

公开(公告)日：2006-05-09

申请号：US10353753

申请日：2003-01-28

申请人： Hun-Taek Kim ,  In-Young Song ,  Jae Won Choi ,  Jin-Wook Jang ,  Yong-Kook Kim ,  Ho Soon Lee ,  Yung-Jue Bang ,  Dae-Kee Kim

发明人： Hun-Taek Kim ,  In-Young Song ,  Jae Won Choi ,  Jin-Wook Jang ,  Yong-Kook Kim ,  Ho Soon Lee ,  Yung-Jue Bang ,  Dae-Kee Kim

IPC分类号： A61K35/14 ,  A61K35/16 ,  A61K38/00

CPC分类号： C07K14/755 ,  A61K48/00 ,  Y10S514/802 ,  Y10S514/834 ,  Y10S930/10

摘要： The application discloses Factor VIII polypeptides comprising internal deletions of amino acids within the area of residues 741 to 1689, wherein the thrombin cleavage sites at about 741 and about 1689 are present, and a site at about 1648 is not present, as compared to human Factor VIII.

摘要翻译： 该应用公开了包含在残基741至1689的区域内的氨基酸的内部缺失的因子VIII多肽，其中存在约741和约1689的凝血酶切割位点，并且与人因子相比不存在约1648位点 八，

公开(公告)号：US6035160A

公开(公告)日：2000-03-07

申请号：US210620

申请日：1998-12-14

申请人： Yong-Kook Kim

发明人： Yong-Kook Kim

IPC分类号： G03G15/20

CPC分类号： G03G15/2032

摘要： A roller separating device automatically separates a pressure roller from a heat roller when the rollers are unable to rotate or when a fixing operation is not in progress. The roller separating device includes an idle gear engaged with a pressure roller gear, a cam driving gear engaged with the idle gear, an electronic clutch fixed to a cam shaft to which a cam is also fixed. The electronic clutch is detachably coupled to the cam driving gear. The cam is driven by a driving force of a power gear and the electronic clutch couples/uncouples the driving force to the cam. When the electronic clutch is actuated, a driving force of the cam driving gear is transferred to the cam to move a pressure roller support frame so that the pressure roller is separated from the heat roller. When the electronic clutch is inactuated, the pressure roller support frame returns to its normal position by an elastic force of a spring so that the pressure roller contacts the heat roller.

摘要翻译： 当辊不能旋转时或当定影操作未进行时，辊分离装置自动将加压辊与加热辊分离。 辊分离装置包括与压辊齿轮接合的空转齿轮，与空转齿轮啮合的凸轮驱动齿轮，固定到凸轮轴的电子离合器，凸轮也固定到凸轮轴上。 电子离合器可拆卸地联接到凸轮驱动齿轮。 凸轮由动力齿轮的驱动力驱动，并且电子离合器将驱动力耦合/解耦到凸轮。 当电子离合器被致动时，凸轮驱动齿轮的驱动力被传递到凸轮以移动压力辊支撑框架，使得加压辊与加热辊分离。 当电子离合器失效时，压力辊支撑架通过弹簧的弹力返回到其正常位置，使得压力辊接触加热辊。

公开(公告)号：US20110316100A1

公开(公告)日：2011-12-29

申请号：US13201112

申请日：2010-02-11

申请人： Yong-Kook Kim ,  Chung-Dam Song

发明人： Yong-Kook Kim ,  Chung-Dam Song

IPC分类号： H01L29/84 ,  H01L21/306 ,  H01L21/20

CPC分类号： H04R19/04 ,  B81B2201/0257 ,  B81B2203/0127 ,  B81C1/00158

摘要： A micro electro mechanical systems (MEMS) microphone, and a method of manufacturing the MEMS microphone having an interval between a membrane and a back plate, the interval being correctly adjusted by forming the membrane and the back plate after an air-gap forming portion on a silicon substrate. Since the membrane and/or the back plate are/is formed by electroless plating, a sacrificial layer is easily planarized, and a residual stress is easily removed or controlled. The MEMS microphone includes a silicon substrate in which a back chamber is formed and on which an air-gap forming portion is formed above the chamber by etching the silicon substrate to a predetermined depth above the chamber; a membrane formed on the air-gap forming portion of the silicon substrate or the silicon substrate; and a back plate that is formed on the air-gap forming portion or the silicon substrate so as to be spaced apart from the membrane, wherein an air gap is formed between the membrane and the back plate.

摘要翻译： 微电子机械系统（MEMS）麦克风以及制造具有隔膜与背板之间间隔的MEMS麦克风的方法，该间隔通过在气隙形成部分上形成膜和背板而正确地调节 硅衬底。 由于膜和/或背板通过无电镀形成，牺牲层容易平坦化，并且容易地去除或控制残余应力。 MEMS麦克风包括硅衬底，其中形成后室，并且通过将硅衬底蚀刻到室上方的预定深度而在腔上方形成气隙形成部分; 形成在硅衬底或硅衬底的气隙形成部分上的膜; 以及形成在所述气隙形成部或所述硅基板上以与所述膜隔开的背板，其中在所述膜与所述背板之间形成气隙。

公开(公告)号：US20110311081A1

公开(公告)日：2011-12-22

申请号：US13201143

申请日：2010-02-11

申请人： Yong-Kook Kim

发明人： Yong-Kook Kim

IPC分类号： H04R1/00 ,  H01L21/02

CPC分类号： B81B7/0016 ,  B81B2201/0257 ,  B81B2203/0109 ,  B81C1/00976 ,  H04R3/007 ,  H04R19/005 ,  H04R19/04 ,  H04R31/00

摘要： A micro electro mechanical system (MEMS) microphone capable of preventing a membrane and a back plate from being contacting each other by an overvoltage, an external shock, and the like, and a method of manufacturing the MEMS microphone. The MEMS microphone includes a silicon substrate in which a back chamber is to be formed; a back plate which is formed on the silicon substrate and has formed therein a plurality of sound holes; a membrane which is formed on the silicon substrate at a predetermined distance apart from the back plate to form an air gap; and a contact-preventing electrode unit which is formed on the silicon substrate and applies a repulsive force to the membrane.

摘要翻译： 能够通过过电压，外部冲击等防止膜和背板彼此接触的微机电系统（MEMS）麦克风，以及制造MEMS麦克风的方法。 MEMS麦克风包括将在其中形成后室的硅衬底; 背板，其形成在所述硅基板上并且在其中形成有多个声孔; 膜，其形成在硅基板上，与背板隔开预定距离，形成气隙; 以及形成在所述硅基板上并对所述膜施加斥力的接触防止电极单元。

公开(公告)号：US09441030B2

公开(公告)日：2016-09-13

申请号：US12528204

申请日：2007-02-23

申请人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-Il Ryu ,  Dae-Kee Kim

发明人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-Il Ryu ,  Dae-Kee Kim

IPC分类号： C07K14/755

CPC分类号： C07K14/755 ,  C07K1/22

摘要： Disclosed is a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate. This invention also relates to a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography.

摘要翻译： 公开了一种通过体外培养哺乳动物细胞产生在无血清培养基中具有因子VIII促凝血活性的蛋白质的方法，其中无血清培养基含有针对由培养细胞释放的蛋白酶的抑制剂。 根据本发明，抑制剂可以保护培养期间靶蛋白的切割并增加目标分子的均匀性，其中抑制剂可以是硫酸葡聚糖。 本发明还涉及通过亲和层析从含有靶分子和选择的抑制剂的培养基中纯化目标分子的方法。

公开(公告)号：US20100112641A1

公开(公告)日：2010-05-06

申请号：US12528204

申请日：2007-02-23

申请人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-II Ryu ,  Dae-Kee Kim

发明人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-II Ryu ,  Dae-Kee Kim

IPC分类号： C12P21/04

CPC分类号： C07K14/755 ,  C07K1/22

摘要： Disclosed is a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate. This invention also relates to a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography.

摘要翻译： 公开了一种通过体外培养哺乳动物细胞产生在无血清培养基中具有因子VIII促凝血活性的蛋白质的方法，其中无血清培养基含有针对由培养细胞释放的蛋白酶的抑制剂。 根据本发明，抑制剂可以保护培养期间靶蛋白的切割并增加目标分子的均匀性，其中抑制剂可以是硫酸葡聚糖。 本发明还涉及通过亲和层析从含有靶分子和选择的抑制剂的培养基中纯化目标分子的方法。