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8 条记录 (耗时 0.03 秒)

    PROCESS FOR PRODUCING AND PURIFYING FACTOR VIII AND ITS DERIVATIVES
    1.
    发明申请
    PROCESS FOR PRODUCING AND PURIFYING FACTOR VIII AND ITS DERIVATIVES 有权
    生产和净化因子VIII及其衍生物的方法

    公开(公告)号：US20100112641A1

    公开(公告)日：2010-05-06

    申请号：US12528204

    申请日：2007-02-23

    申请人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-II Ryu ,  Dae-Kee Kim

    发明人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-II Ryu ,  Dae-Kee Kim

    IPC分类号： C12P21/04

    CPC分类号： C07K14/755 ,  C07K1/22

    摘要： Disclosed is a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate. This invention also relates to a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography.

    摘要翻译： 公开了一种通过体外培养哺乳动物细胞产生在无血清培养基中具有因子VIII促凝血活性的蛋白质的方法，其中无血清培养基含有针对由培养细胞释放的蛋白酶的抑制剂。 根据本发明，抑制剂可以保护培养期间靶蛋白的切割并增加目标分子的均匀性，其中抑制剂可以是硫酸葡聚糖。 本发明还涉及通过亲和层析从含有靶分子和选择的抑制剂的培养基中纯化目标分子的方法。

    Process for producing and purifying factor VIII and its derivatives
    2.
    发明授权
    Process for producing and purifying factor VIII and its derivatives 有权
    生产和净化因子VIII及其衍生物的方法

    公开(公告)号：US09441030B2

    公开(公告)日：2016-09-13

    申请号：US12528204

    申请日：2007-02-23

    申请人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-Il Ryu ,  Dae-Kee Kim

    发明人： In-Young Song ,  Hun-Taek Kim ,  Jong-Wan Kim ,  Yong-Kook Kim ,  Jong-Il Ryu ,  Dae-Kee Kim

    IPC分类号： C07K14/755

    CPC分类号： C07K14/755 ,  C07K1/22

    摘要： Disclosed is a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate. This invention also relates to a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography.

    摘要翻译： 公开了一种通过体外培养哺乳动物细胞产生在无血清培养基中具有因子VIII促凝血活性的蛋白质的方法，其中无血清培养基含有针对由培养细胞释放的蛋白酶的抑制剂。 根据本发明，抑制剂可以保护培养期间靶蛋白的切割并增加目标分子的均匀性，其中抑制剂可以是硫酸葡聚糖。 本发明还涉及通过亲和层析从含有靶分子和选择的抑制剂的培养基中纯化目标分子的方法。

    IMPROVEMENT APPARATUS OF SURFACE ROUGHNESS DEFECT OF HOT/COLD ROLLED STAINLESS STEEL COILS AND THE METHOD THEREOF
    7.
    发明申请
    IMPROVEMENT APPARATUS OF SURFACE ROUGHNESS DEFECT OF HOT/COLD ROLLED STAINLESS STEEL COILS AND THE METHOD THEREOF 审中-公开
    热/冷轧不锈钢线圈的表面粗糙度缺陷的改进设备及其方法

    公开(公告)号：US20100101064A1

    公开(公告)日：2010-04-29

    申请号：US12516156

    申请日：2007-09-12

    申请人： Jong-Wan Kim ,  Jung-Ki Kim ,  Joung-Mo Lee ,  Dong-Sik Kim

    发明人： Jong-Wan Kim ,  Jung-Ki Kim ,  Joung-Mo Lee ,  Dong-Sik Kim

    IPC分类号： B21B45/00 ,  B21B45/08 ,  B21B45/04

    CPC分类号： B21B45/08 ,  B21B3/02 ,  Y10T29/4544 ,  Y10T29/4561 ,  Y10T29/4567 ,  Y10T83/242

    摘要： Disclosed is to an improvement apparatus of surface roughness of hot/cold rolled stainless steel coils and the method thereof. The present invention provides an improvement apparatus of surface roughness of hot/cold rolled stainless steel coils including a water spray unit for spraying water on a surface of a cast stainless steel slab; a cutting unit for cutting the stainless steel slab; and a high-pressure water spray having at least one nozzle in a region where the stainless steel slab is cut by the cutting unit and discharged and installed to be symmetric in upper and lower parts of the stainless steel slab; and a roller brush installed to be in contact with and symmetric in the upper and lower parts of the stainless steel slab passed through the water spray so as to rotate at a high speed. Accordingly, the improvement apparatus according to the present invention may be useful to produce a stainless steel slab having an excellent surface quality since it is possible to easily remove the scale and mold slag from the stainless steel slab.

    摘要翻译： 本发明公开了一种热轧不锈钢卷材表面粗糙度的改进装置及其制造方法。 本发明提供了一种包括用于在铸造不锈钢板的表面上喷水的喷水单元的热/冷轧不锈钢卷材表面粗糙度的改进装置， 用于切割不锈钢板的切割单元; 以及在不锈钢板被切割单元切割的区域中具有至少一个喷嘴的高压喷水器，并且在不锈钢板的上部和下部排出并安装成对称的区域; 以及安装成与穿过喷水头的不锈钢板的上部和下部接触并对称的辊刷，以便高速旋转。 因此，根据本发明的改进装置可以用于生产具有优异表面质量的不锈钢板，因为可以容易地从不锈钢板上去除垢和模具渣。

    On-Line Quality Prediction System for Stainless Steel Slab and the Predicting Method Using It
    8.
    发明申请
    On-Line Quality Prediction System for Stainless Steel Slab and the Predicting Method Using It 审中-公开
    不锈钢板在线质量预测系统及其预测方法

    公开(公告)号：US20090138223A1

    公开(公告)日：2009-05-28

    申请号：US11992736

    申请日：2006-09-27

    申请人： Jong-Wan Kim ,  Ja-Yong Choi ,  Bo-Sung Seo

    发明人： Jong-Wan Kim ,  Ja-Yong Choi ,  Bo-Sung Seo

    IPC分类号： G06F19/00

    CPC分类号： G05B19/41875 ,  B22D11/16 ,  G05B2219/32194 ,  Y02P90/18 ,  Y02P90/22

    摘要： Disclosed is an on-line quality prediction system for stainless steel slab and the predicting method using it, which can allow produced slab quality to predict in high precision on the on-line using a network based system by collecting all operation data available from a steel making process to a continuous casting process and then using them as a metallurgical calculation evaluating model through thermodynamics and statistics programs, the system comprises: a main computer collecting and storing information from a production line for the stainless steel slab; a thermodynamics calculation only computer mutually communicating with the main computer; and a server computer mutually communicating with the main computer, whereby it can overcome a limitation of a predicting method due to existing operation data and allow produced slab quality to predict in high precision on the on-line using a network based system by collecting all operation data available from a steel making process to a continuous casting process and then using them as a metallurgical calculation evaluating model through thermodynamics and statistics programs, significantly improving quality and productivity.

    摘要翻译： 公开了一种用于不锈钢板的在线质量预测系统及其使用的预测方法，其可以使用基于网络的系统在线准确地预测制造的板坯质量，通过收集钢中可获得的所有操作数据 制造过程进行连续铸造，然后通过热力学和统计程序将其用作冶金计算评估模型，该系统包括：收集和存储不锈钢板生产线信息的主计算机; 仅与计算机相互通信的热力学计算机; 以及与主计算机相互通信的服务器计算机，由此可以克服由于现有操作数据引起的预测方法的限制，并且通过收集所有操作，允许生产的平板质量在网上使用基于网络的系统在线准确地预测 从制钢过程到连续铸造过程的数据，然后通过热力学和统计程序将其用作冶金计算评估模型，显着提高质量和生产率。