公开(公告)号：US20050019790A1

公开(公告)日：2005-01-27

申请号：US10777585

申请日：2004-02-12

申请人： Yoshihide Iwaki ,  Yoshihiko Makino ,  Hiroshi Shinoki ,  Satoru Kuhara ,  Kosuke Tashiro ,  Shigeru Muta

发明人： Yoshihide Iwaki ,  Yoshihiko Makino ,  Hiroshi Shinoki ,  Satoru Kuhara ,  Kosuke Tashiro ,  Shigeru Muta

IPC分类号： G01N33/53 ,  B01J19/00 ,  C12M1/00 ,  C12N15/09 ,  C40B40/06 ,  C40B60/14 ,  G01N33/566 ,  G01N37/00 ,  C12Q1/68 ,  B05D3/00 ,  C12M1/34

CPC分类号： B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

摘要： A micro-array for analysis of DNA is prepared by the steps of spotting onto a solid carrier in its predetermined area in which plural reactive groups are fixed an aqueous solution which contains a thickening agent and probe molecules (e.g., DNA fragments) having a group reactive with the reactive group of the solid carrier to produce covalent bonding; spotting onto the solid carrier in a different area having the same reactive groups an aqueous solution (same or different); incubating the aqueous solution-spotted solid carrier to produce the covalent bondings; and washing the solid carrier with an aqueous medium to remove the thickening agent from the solid carrier. An electrostatic bonding can be utilized in place of the covalent bonding.

摘要翻译： 用于分析DNA的微阵列通过以下步骤制备：将固定载体固定在其中固定有多个反应性基团的预定区域的固体载体上，所述固定载体含有增稠剂的水溶液和具有基团的探针分子（例如，DNA片段） 与固体载体的反应性基团反应产生共价键; 在具有相同反应性基团的不同区域的固体载体上发现水溶液（相同或不同）; 孵育水溶液点固体载体以产生共价键; 并用水介质洗涤固体载体以从固体载体中除去增稠剂。 可以使用静电键来代替共价键。

公开(公告)号：US06871427B2

公开(公告)日：2005-03-29

申请号：US10287660

申请日：2002-11-05

申请人： Shigeru Muta ,  Kazuyuki Miyazaki

发明人： Shigeru Muta ,  Kazuyuki Miyazaki

IPC分类号： B66C23/74 ,  E02F9/18 ,  B66C13/00

CPC分类号： E02F9/18 ,  B66C23/74

摘要： The construction machine according to the present invention comprises a lower running body, an upper turning body mounted free to turn on the lower running body, and a connecting bracket arranged between the rear end of a turning frame of the upper turning body and a rear device arranged on the rear end side, characterized in that the connecting bracket is provided with a connecting portion connected detachably to the rear end of the turning frame, and a mounting portion for detachably mounting the rear device such as a counterweight elevating device. Thereby, the construction machine can be applied to multi-purpose uses while using the frame construction in common.

摘要翻译： 根据本发明的施工机械包括下行进体，可自由转动下行走体的上转体，以及设置在上转体本体的转动架的后端和后装置之间的连接支架 其特征在于，所述连接托架设置有可拆卸地连接到所述车削框架的后端的连接部，以及用于可拆卸地安装所述后部装置（例如配重升降装置）的安装部。 因此，在使用框架结构时，施工机械可以应用于多用途。