Microorganism isolated from chinese elm (Ulmus sp.) and process for preparing exopolysaccharides by employing the microorganism
    3.
    发明授权
    Microorganism isolated from chinese elm (Ulmus sp.) and process for preparing exopolysaccharides by employing the microorganism 失效
    从榆树(Ulmus sp。)分离的微生物和使用微生物制备胞外多糖的方法

    公开(公告)号:US06727081B2

    公开(公告)日:2004-04-27

    申请号:US09921013

    申请日:2001-07-27

    IPC分类号: C12P1904

    摘要: The present invention relates to a novel Enterobacter sp. strain SSYL deposited under accession number KCTC 0687BP isolated from the root bark of Chinese elm, which produces immunostimulating exopolysaccharides with anticancer activity, a process for preparing the exopolysaccharides by fermenting the said microorganism in a culture medium, exopolysaccharides prepared by the process and their uses thereof. The exopolysaccharides of the invention have a molecular weight of 100,000 to 1,000,000 and consist of 40-75% of total sugar, 5-15% of total acidic sugar and 10-25% of total protein. The exopolysaccharides exhibits a high immunoenhancing activity in immune cell proliferation, direct mitogenicity and mixed lymphocyte reaction, and further a high anticancer activity in vivo by virtue of immunostimulation. Moreover, the production of the exopolysaccharides by fermentation of a microorganism, makes it possible to provide the exopolysaccharides with a uniform quality and mass production without destruction of the plant species. The exopolysaccharides of the subject invention have practical uses as an active ingredient for anticancer agents, immunoenhancers and foodstuffs.

    摘要翻译: 本发明涉及一种新型肠杆菌属 以保藏号为KCTC 0687BP的中国榆皮根分离的菌株SSYL,其产生具有抗癌活性的免疫刺激性外多糖,通过在培养基中发酵所述微生物制备外多糖的方法,通过该方法制备的外多糖及其用途 。 本发明的外多糖的分子量为100,000至1,000,000,由总糖的40-75%,总酸性糖的5-15%和总蛋白的10-25%组成。 外多糖在免疫细胞增殖,直接有丝分裂原和混合淋巴细胞反应中表现出高的免疫增强活性,并通过免疫刺激进一步提高体内抗癌活性。 此外,通过微生物的发酵生产外多糖,使得可以提供均匀质量和大量生产的外多糖而不破坏植物物种。 本发明的外多糖作为抗癌剂,免疫增强剂和食品的活性成分具有实用性。