公开(公告)号：US20070218471A1

公开(公告)日：2007-09-20

申请号：US11550099

申请日：2006-10-17

申请人： Young Rok Kim ,  Jun Hong Min ,  In Ho Lee ,  Ah gi Kim ,  Kui Hyun Kim

发明人： Young Rok Kim ,  Jun Hong Min ,  In Ho Lee ,  Ah gi Kim ,  Kui Hyun Kim

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： G01N33/48721 ,  C12Q1/6825 ,  C12Q2565/631

摘要： Disclosed herein is a method for detecting DNA using a nanopore including treating the surface of a nanopore formed in a solid substrate with a substance carrying positive charges; introducing a DNA-containing sample into the surface-treated nanopore; and detecting electrical signals generated during translocation of the sample through the nanopore. Also disclosed herein is device for detecting DNA using a nanopore including a solid substrate including a nanopore, treated with a substance which carries positive charges to change a surface property of the nanopore so that the nanopore surface carries positive charges; an electrode applying voltage to the nanopore of the solid substrate; and a measurement unit measuring an electrical signal generated during translocation of a DNA-containing sample through the nanopore.

摘要翻译： 本文公开了一种使用纳米孔检测DNA的方法，包括用携带正电荷的物质处理在固体基质中形成的纳米孔的表面; 将含DNA样品引入表面处理的纳米孔中; 并检测通过纳米孔在样品易位期间产生的电信号。 本文还公开了使用纳米孔检测DNA的装置，所述纳米孔包括包含纳米孔的固体基质，用携带正电荷的物质处理以改变纳米孔的表面性质，使得纳米孔表面带有正电荷; 电极向固体基底的纳米孔施加电压; 以及测量单元，其测量通过纳米孔在含DNA样品的易位期间产生的电信号。

公开(公告)号：US07943369B2

公开(公告)日：2011-05-17

申请号：US11517090

申请日：2006-09-07

申请人： In Ho Lee ,  Jun Hong Min ,  Su Hyeon Kim ,  Chin Sung Park ,  Ah gi Kim ,  Kui Hyun Kim

发明人： In Ho Lee ,  Jun Hong Min ,  Su Hyeon Kim ,  Chin Sung Park ,  Ah gi Kim ,  Kui Hyun Kim

IPC分类号： C12M1/34

CPC分类号： G01N33/5308

摘要： A DNA (Deoxyribo Nucleic Acid) detection device and a manufacturing method thereof detects DNA by an electrical method without a separate process for detection by using semiconductor microfabrication techniques. The DNA detection device includes a pair of chambers formed on a semiconductor substrate for accommodating a detection sample, a channel connecting the pair of chambers and a lid covering the pair of chambers. According to the present invention, it is possible to attain a DNA detection device, which can be mass-produced from a silicon substrate by using semiconductor manufacturing technology with improved microfabrication techniques, and a manufacturing method thereof.

摘要翻译： DNA（脱氧核糖核酸）检测装置及其制造方法，通过使用半导体微细加工技术，不用单独的检测方法，通过电气方式检测DNA。 DNA检测装置包括形成在用于容纳检测样品的半导体基板上的一对室，连接该对室的通道和覆盖该对室的盖。 根据本发明，可以通过使用具有改进的微细加工技术的半导体制造技术来获得可以从硅基板批量生产的DNA检测装置及其制造方法。