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公开(公告)号：US07943369B2
公开(公告)日：2011-05-17
申请号：US11517090
申请日：2006-09-07
申请人： In Ho Lee , Jun Hong Min , Su Hyeon Kim , Chin Sung Park , Ah gi Kim , Kui Hyun Kim
发明人： In Ho Lee , Jun Hong Min , Su Hyeon Kim , Chin Sung Park , Ah gi Kim , Kui Hyun Kim
IPC分类号： C12M1/34
CPC分类号： G01N33/5308
摘要： A DNA (Deoxyribo Nucleic Acid) detection device and a manufacturing method thereof detects DNA by an electrical method without a separate process for detection by using semiconductor microfabrication techniques. The DNA detection device includes a pair of chambers formed on a semiconductor substrate for accommodating a detection sample, a channel connecting the pair of chambers and a lid covering the pair of chambers. According to the present invention, it is possible to attain a DNA detection device, which can be mass-produced from a silicon substrate by using semiconductor manufacturing technology with improved microfabrication techniques, and a manufacturing method thereof.
摘要翻译： DNA（脱氧核糖核酸）检测装置及其制造方法，通过使用半导体微细加工技术，不用单独的检测方法，通过电气方式检测DNA。 DNA检测装置包括形成在用于容纳检测样品的半导体基板上的一对室，连接该对室的通道和覆盖该对室的盖。 根据本发明，可以通过使用具有改进的微细加工技术的半导体制造技术来获得可以从硅基板批量生产的DNA检测装置及其制造方法。
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2.发明申请METHOD FOR HIGHLY SENSITIVE NUCLEIC ACID DETECTION USING NANOPORE AND NON-SPECIFIC NUCLEIC ACID BINDING AGENT 审中-公开使用纳米和非特异性核酸结合剂进行敏感性核酸检测的方法公开(公告)号：US20090169431A1
公开(公告)日：2009-07-02
申请号：US12364235
申请日：2009-02-02
申请人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
发明人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
IPC分类号： G01N27/00
CPC分类号： B82Y5/00 , C12Q1/6825 , Y10S436/806 , Y10T436/143333 , C12Q2565/631
摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.
摘要翻译： 提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。 可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。
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3.发明申请PRIMER SET FOR AMPLIFYING TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, PROBE OR PROBE SET SPECIFICALLY HYBRIDIZING WITH TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, METHOD OF DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES USING THE PROBE OR PROBE SET, AND KIT FOR DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES 审中-公开用于放大抗生素细菌物种的目标序列的初步设置，特异性地与抗生素耐性细菌物种的目标序列混合的探针或探针，使用探针或探针组检测抗生素耐性细菌物种的方法， 和用于检测抗生素细菌物种的工具包公开(公告)号：US20090163382A1
公开(公告)日：2009-06-25
申请号：US11863984
申请日：2007-09-28
申请人： Ji-Young OH , Yeon-su LEE , Sang-hyun PAEK , Byung-chul KIM , Sook-young KIM , Kyung-hee PARK , Jung-nam LEE , Jong-suk CHUNG , Ah-gi KIM , Myo-yong LEE , Tae-jin AHN
发明人： Ji-Young OH , Yeon-su LEE , Sang-hyun PAEK , Byung-chul KIM , Sook-young KIM , Kyung-hee PARK , Jung-nam LEE , Jong-suk CHUNG , Ah-gi KIM , Myo-yong LEE , Tae-jin AHN
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Provided are a primer set for amplifying target sequence(s) of antibiotic-resistant bacterial species, a probe or probe set specifically hybridizing with target sequence(s) of antibiotic-resistant bacterial species, a microarray immobilized with the probe or probe set, a kit comprising the primer set and a method of detecting at least one antibiotic-resistant bacterial species using the probe or probe set.
摘要翻译： 提供了用于扩增抗生素抗性细菌种类的靶序列的引物组，与抗生素抗性细菌种类的靶序列特异性杂交的探针或探针组，固定有探针或探针组的微阵列 包括引物组的试剂盒和使用探针或探针组检测至少一种抗生素抗性细菌物种的方法。
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4.发明授权Method for highly sensitive nucleic acid detection using nanopore and non-specific nucleic acid-binding agent 失效使用纳米孔和非特异性核酸结合剂的高灵敏度核酸检测方法公开(公告)号：US07504261B2
公开(公告)日：2009-03-17
申请号：US11414022
申请日：2006-04-28
申请人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
发明人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
CPC分类号： B82Y5/00 , C12Q1/6825 , Y10S436/806 , Y10T436/143333 , C12Q2565/631
摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.
摘要翻译： 提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。 可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。
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公开(公告)号：US20060292605A1
公开(公告)日：2006-12-28
申请号：US11414022
申请日：2006-04-28
申请人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
发明人： Kui-hyun Kim , Jun-hong Min , In-ho Lee , Ah-gi Kim
CPC分类号： B82Y5/00 , C12Q1/6825 , Y10S436/806 , Y10T436/143333 , C12Q2565/631
摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.
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6.发明申请公开(公告)号：US20100267011A1
公开(公告)日：2010-10-21
申请号：US11499052
申请日：2006-08-04
申请人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
发明人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
CPC分类号： B82Y30/00 , C12Q1/6816 , C12Q2563/149 , C12Q2525/131 , C12Q2521/514
摘要： There are provided a method and apparatus for detecting nucleic acid using bead and nanopore, and more specifically, a method and apparatus capable of detecting nucleic acid fragments of 70 bps to 300 bps in length by a nanopore detection unit with nanopores of 20 to 120 nm in diameter by attaching a bead to a nucleic acid probe and then detecting the bead attached to nucleic acid not nucleic acid itself. Accordingly, the present invention can detect the nucleic acid fragments using the nanopore detection unit with nanopores of 20 to 120 nm in diameter, even in case where Polymerase Chain Reaction (PCR) products are given as the sample, particularly the PCR products are the nucleic acid fragments of 70 to 300 bps in length.
摘要翻译： 提供了使用珠粒和纳米孔检测核酸的方法和装置，更具体地，可以通过具有20至120nm的纳米孔的纳米孔检测单元来检测长度为70bps至300bps的核酸片段的方法和装置 通过将珠附着到核酸探针上，然后检测附着于核酸而不是核酸本身的珠。 因此，即使在给出聚合酶链反应（PCR）产物作为样品的情况下，本发明也可以使用直径为20-120nm的纳米孔的纳米孔检测单元来检测核酸片段，特别是PCR产物是核酸 长度为70至300 bps的酸性片段。
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7.发明申请METHOD AND COMPOSITIONS FOR DETECTING RESPIRATORY DISEASE-CAUSING BACTERIAL SPECIES 有权用于检测呼吸道疾病细菌物种的方法和组合物公开(公告)号：US20080081770A1
公开(公告)日：2008-04-03
申请号：US11862758
申请日：2007-09-27
申请人： Ji-young OH , Sang-hyun PAEK , Kyung-hee PARK , Jung-nam LEE , Jong-suk CHUNG , Ah-gi KIM
发明人： Ji-young OH , Sang-hyun PAEK , Kyung-hee PARK , Jung-nam LEE , Jong-suk CHUNG , Ah-gi KIM
IPC分类号： C40B40/08
CPC分类号： C12Q1/689 , C12Q2600/16 , Y02A50/451
摘要： Provided are a primer set for specifically amplifying target sequence(s) of twelve respiratory disease-causing bacterial species, a probe set specifically hybridizing with target sequence(s) of twelve respiratory disease-causing bacterial species, a microarray comprising the probe set, and a method of detecting a respiratory disease-causing bacterial species using the probe set.
摘要翻译： 提供了用于特异性扩增十二种呼吸道性疾病的细菌种类的靶序列的引物组，与12种呼吸道性疾病致细菌物种的靶序列特异性杂交的探针组，包含探针组的微阵列和 使用探针组检测呼吸系统疾病的细菌物种的方法。
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8.发明授权Method and compositions for detecting respiratory disease-causing bacterial species 有权用于检测呼吸道性疾病的细菌物种的方法和组合物公开(公告)号：US07659388B2
公开(公告)日：2010-02-09
申请号：US11862758
申请日：2007-09-27
申请人： Ji-young Oh , Sang-hyun Paek , Kyung-hee Park , Jung-nam Lee , Jong-suk Chung , Ah-gi Kim
发明人： Ji-young Oh , Sang-hyun Paek , Kyung-hee Park , Jung-nam Lee , Jong-suk Chung , Ah-gi Kim
CPC分类号： C12Q1/689 , C12Q2600/16 , Y02A50/451
摘要： Provided are a primer set for specifically amplifying target sequence(s) of twelve respiratory disease-causing bacterial species, a probe set specifically hybridizing with target sequence(s) of twelve respiratory disease-causing bacterial species, a microarray comprising the probe set, and a method of detecting a respiratory disease-causing bacterial species using the probe set.
摘要翻译： 提供了用于特异性扩增十二种呼吸道性疾病的细菌种类的靶序列的引物组，与12种呼吸道性疾病致细菌物种的靶序列特异性杂交的探针组，包含探针组的微阵列和 使用探针组检测呼吸系统疾病的细菌物种的方法。
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公开(公告)号：US08431337B2
公开(公告)日：2013-04-30
申请号：US11499052
申请日：2006-08-04
申请人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
发明人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
CPC分类号： B82Y30/00 , C12Q1/6816 , C12Q2563/149 , C12Q2525/131 , C12Q2521/514
摘要： There are provided a method and apparatus for detecting nucleic acid using bead and nanopore, and more specifically, a method and apparatus capable of detecting nucleic acid fragments of 70 bps to 300 bps in length by a nanopore detection unit with nanopores of 20 to 120 nm in diameter by attaching a bead to a nucleic acid probe and then detecting the bead attached to nucleic acid not nucleic acid itself. Accordingly, the present invention can detect the nucleic acid fragments using the nanopore detection unit with nanopores of 20 to 120 nm in diameter, even in case where Polymerase Chain Reaction (PCR) products are given as the sample, particularly the PCR products are the nucleic acid fragments of 70 to 300 bps in length.
摘要翻译： 提供了使用珠粒和纳米孔检测核酸的方法和装置，更具体地，可以通过具有20至120nm的纳米孔的纳米孔检测单元来检测长度为70bps至300bps的核酸片段的方法和装置 通过将珠附着到核酸探针上，然后检测附着于核酸而不是核酸本身的珠。 因此，即使在给出聚合酶链反应（PCR）产物作为样品的情况下，本发明也可以使用直径为20-120nm的纳米孔的纳米孔检测单元来检测核酸片段，特别是PCR产物是核酸 长度为70至300 bps的酸性片段。
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10.发明申请公开(公告)号：US20070218471A1
公开(公告)日：2007-09-20
申请号：US11550099
申请日：2006-10-17
申请人： Young Rok Kim , Jun Hong Min , In Ho Lee , Ah gi Kim , Kui Hyun Kim
发明人： Young Rok Kim , Jun Hong Min , In Ho Lee , Ah gi Kim , Kui Hyun Kim
CPC分类号： G01N33/48721 , C12Q1/6825 , C12Q2565/631
摘要： Disclosed herein is a method for detecting DNA using a nanopore including treating the surface of a nanopore formed in a solid substrate with a substance carrying positive charges; introducing a DNA-containing sample into the surface-treated nanopore; and detecting electrical signals generated during translocation of the sample through the nanopore. Also disclosed herein is device for detecting DNA using a nanopore including a solid substrate including a nanopore, treated with a substance which carries positive charges to change a surface property of the nanopore so that the nanopore surface carries positive charges; an electrode applying voltage to the nanopore of the solid substrate; and a measurement unit measuring an electrical signal generated during translocation of a DNA-containing sample through the nanopore.
摘要翻译： 本文公开了一种使用纳米孔检测DNA的方法，包括用携带正电荷的物质处理在固体基质中形成的纳米孔的表面; 将含DNA样品引入表面处理的纳米孔中; 并检测通过纳米孔在样品易位期间产生的电信号。 本文还公开了使用纳米孔检测DNA的装置，所述纳米孔包括包含纳米孔的固体基质，用携带正电荷的物质处理以改变纳米孔的表面性质，使得纳米孔表面带有正电荷; 电极向固体基底的纳米孔施加电压; 以及测量单元，其测量通过纳米孔在含DNA样品的易位期间产生的电信号。
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