公开(公告)号：US20080076189A1

公开(公告)日：2008-03-27

申请号：US11694605

申请日：2007-03-30

申请人： Yuri Belosludtsev ,  Nasanshargal Battulga ,  Mistu Reddy ,  Anelia Kraltcheva ,  Susan Hardin ,  Tommie Lincecum ,  Hongyi Wang ,  Norha Deluge ,  Uma Nagaswamy ,  Benjamin Stevens ,  Kristi Kincaid

发明人： Yuri Belosludtsev ,  Nasanshargal Battulga ,  Mistu Reddy ,  Anelia Kraltcheva ,  Susan Hardin ,  Tommie Lincecum ,  Hongyi Wang ,  Norha Deluge ,  Uma Nagaswamy ,  Benjamin Stevens ,  Kristi Kincaid

IPC分类号： G01N21/64

CPC分类号： G01N21/6428

摘要： Support surfaces are disclosed that are designed to support molecules or molecular assemblies immobilized thereon so that the molecules or molecular assemblies can be observed in single molecule detections systems, where the support surfaces have reduced background and the fluorescent labels associated with the immobilized molecules or molecular assemblies have longer active lifetimes prior to permanent photo-bleaching or deactivation and have improve fluorescence properties and where the surfaces have more uniform fluorescent properties.

摘要翻译： 公开了支撑表面，其被设计成支持固定在其上的分子或分子组件，使得可以在单分子检测系统中观察分子或分子组件，其中支持表面具有降低的背景，并且与固定化分子或分子组装相关联的荧光标记 在永久性光漂白或去活化之前具有更长的活性寿命，并且具有改善的荧光性能以及其表面具有更均匀的荧光性质。

公开(公告)号：US20110165652A1

公开(公告)日：2011-07-07

申请号：US12812952

申请日：2009-01-14

申请人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

发明人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

IPC分类号： C12N9/12

CPC分类号： C07H19/207 ,  C07H19/10 ,  C12N9/1252 ,  C12Q1/6869

摘要： Compositions, systems and methods of sequencing are disclosed, where the compositions and systems include polymerase enzymes that have been genetically modified to more efficiently incorporate nucleotides including labels having a detectable properties that are released during incorporation, to augment a rate of labeled nucleotide incorporation, to augment a rate of pyrophosphate release, or to augment two or more of these properties and rates. Also disclosed are terminally labeled and dual labeled nucleotides, and click-chemistry based methods of synthesizing the same.

摘要翻译： 公开了组合物，系统和测序方法，其中组合物和系统包括经遗传修饰以更有效地掺入核苷酸的聚合酶，包括在掺入期间释放的具有可检测性质的标记，以增加标记的核苷酸掺入率， 增加焦磷酸盐释放的速率，或增加这些性质和速率中的两种或更多种。 还公开了末端标记和双重标记的核苷酸，以及基于点击化学的合成方法。

公开(公告)号：US20070250274A1

公开(公告)日：2007-10-25

申请号：US11671956

申请日：2007-02-06

申请人： Andrei Volkov ,  Costa Colbert ,  Ivan Pan ,  Anelia Kraltcheva ,  Mitsu Reddy ,  Nasanshargal Battulga ,  Michael Rea ,  Keun Lee ,  Susan Hardin ,  Brent Mulder ,  Chris Hebel ,  Alok Bandekar

发明人： Andrei Volkov ,  Costa Colbert ,  Ivan Pan ,  Anelia Kraltcheva ,  Mitsu Reddy ,  Nasanshargal Battulga ,  Michael Rea ,  Keun Lee ,  Susan Hardin ,  Brent Mulder ,  Chris Hebel ,  Alok Bandekar

IPC分类号： G01N31/00

CPC分类号： C12Q1/6813 ,  G01N21/6408 ,  G01N21/6456 ,  G01N2021/6439

摘要： A method to be implemented on or in a computer is disclosed, where the method includes data collection, calibration, candidate selection, and analysis of data streams associated with each candidate to classify single molecule fluorescence resonance energy transfer events. Once classified, the classification can be related to the nature of the events, such as the identification of dNTP incorporation during primer extension to obtain a base read out of an unknown template.

摘要翻译： 公开了一种在计算机上或计算机中实现的方法，其中该方法包括与每个候选物相关联的数据流的数据收集，校准，候选选择和分析，以分类单分子荧光共振能量传递事件。 一旦分类，分类可以与事件的性质相关，例如在引物延伸期间鉴定dNTP并入以获得从未知模板读出的碱基。

公开(公告)号：US07668697B2

公开(公告)日：2010-02-23

申请号：US11671956

申请日：2007-02-06

申请人： Andrei Volkov ,  Costa M. Colbert ,  Ivan Pan ,  Anelia Kraltcheva ,  Mitsu Reddy ,  Nasanshargal Battulga ,  Michael A. Rea ,  Keun Woo Lee ,  Susan H. Hardin ,  Brent Mulder ,  Chris Hebel ,  Alok Bandekar

发明人： Andrei Volkov ,  Costa M. Colbert ,  Ivan Pan ,  Anelia Kraltcheva ,  Mitsu Reddy ,  Nasanshargal Battulga ,  Michael A. Rea ,  Keun Woo Lee ,  Susan H. Hardin ,  Brent Mulder ,  Chris Hebel ,  Alok Bandekar

IPC分类号： G06F19/00 ,  G06F17/40 ,  G01N33/00

CPC分类号： C12Q1/6813 ,  G01N21/6408 ,  G01N21/6456 ,  G01N2021/6439

摘要： A method to be implemented on or in a computer is disclosed, where the method includes data collection, calibration, candidate selection, and analysis of data streams associated with each candidate to classify single molecule fluorescence resonance energy transfer events. Once classified, the classification can be related to the nature of the events, such as the identification of dNTP incorporation during primer extension to obtain a base read out of an unknown template.

摘要翻译： 公开了一种在计算机上或计算机中实现的方法，其中该方法包括与每个候选物相关联的数据流的数据收集，校准，候选选择和分析，以分类单分子荧光共振能量传递事件。 一旦分类，分类可以与事件的性质相关，例如在引物延伸期间鉴定dNTP并入以获得从未知模板读出的碱基。

公开(公告)号：US20100235105A1

公开(公告)日：2010-09-16

申请号：US12686372

申请日：2010-01-12

申请人： Andrei Volkov ,  Costa M. Colbert ,  Ivan Pan ,  Anelia Kraltcheva ,  Mitsu Reddy ,  Nasanshargal Battulga ,  Michael A. Rea ,  Keun Woo Lee ,  Susan H. Hardin ,  Brent Mulder ,  Chris Hebel ,  Alok Bandekar

发明人： Andrei Volkov ,  Costa M. Colbert ,  Ivan Pan ,  Anelia Kraltcheva ,  Mitsu Reddy ,  Nasanshargal Battulga ,  Michael A. Rea ,  Keun Woo Lee ,  Susan H. Hardin ,  Brent Mulder ,  Chris Hebel ,  Alok Bandekar

IPC分类号： G06F19/00

CPC分类号： C12Q1/6813 ,  G01N21/6408 ,  G01N21/6456 ,  G01N2021/6439

摘要： A method to be implemented on or in a computer is disclosed, where the method includes data collection, calibration, candidate selection, and analysis of data streams associated with each candidate to classify single molecule fluorescence resonance energy transfer events. Once classified, the classification can be related to the nature of the events, such as the identification of dNTP incorporation during primer extension to obtain a base read out of an unknown template.

摘要翻译： 公开了一种在计算机上或计算机中实现的方法，其中该方法包括与每个候选物相关联的数据流的数据收集，校准，候选选择和分析，以分类单分子荧光共振能量传递事件。 一旦分类，分类可以与事件的性质相关，例如在引物延伸期间鉴定dNTP并入以获得从未知模板读出的碱基。