公开(公告)号：US08512987B2

公开(公告)日：2013-08-20

申请号：US12545966

申请日：2009-08-24

申请人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/24

CPC分类号： C12P13/08 ,  C12P13/04

摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

公开(公告)号：US20100047878A1

公开(公告)日：2010-02-25

申请号：US12545966

申请日：2009-08-24

申请人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/24 ,  C12P13/04 ,  C12P13/14 ,  C12P13/08 ,  C12P13/06 ,  C12P13/22 ,  C12P13/12 ,  C12P13/10 ,  C12P13/20

CPC分类号： C12P13/08 ,  C12P13/04

摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

公开(公告)号：US20060154289A1

公开(公告)日：2006-07-13

申请号：US11337580

申请日：2006-01-24

申请人： Stephen Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

发明人： Stephen Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

IPC分类号： C12Q1/68 ,  C12Q1/18 ,  C12N1/21

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

摘要翻译： 影响使用细胞的物质生产的代谢通量是通过以下方法确定的：1）根据基质通过所需生产物质的生物化学反应的公式产生化学计量矩阵，2）从所有代谢通量中选择相同数量的独立代谢通量， 作为自由通量的化学计量矩阵的自由度，3）产生足够数量的自由通量的随机组合用于统计分析，并且基于化学计量矩阵计算每个创建的组合的代谢通量分布，4）获得包括 通过多变量统计分析从计算的代谢通量分布中显示与物质生产相关的最小数量的自由通量，以及5）基于所获得的回归方程中的系数确定影响物质生产的至少一种代谢通量。

公开(公告)号：US08030036B2

公开(公告)日：2011-10-04

申请号：US11877726

申请日：2007-10-24

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

IPC分类号： C12P13/08 ,  C12N9/04 ,  C12N1/21 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

公开(公告)号：US07306933B2

公开(公告)日：2007-12-11

申请号：US11275437

申请日：2006-01-03

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

IPC分类号： C12P13/08 ,  C12N9/04 ,  C12N1/21 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

公开(公告)号：US07809511B2

公开(公告)日：2010-10-05

申请号：US11337580

申请日：2006-01-24

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

IPC分类号： G06F19/00

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

摘要翻译： 影响使用细胞的物质生产的代谢通量是通过以下方法确定的：1）根据基质通过所需生产物质的生物化学反应的公式产生化学计量矩阵，2）从所有代谢通量中选择相同数量的独立代谢通量， 作为自由通量的化学计量矩阵的自由度，3）产生足够数量的自由通量的随机组合用于统计分析，并且基于化学计量矩阵计算每个创建的组合的代谢通量分布，4）获得包括 通过多变量统计分析从计算的代谢通量分布中显示与物质生产相关的最小数量的自由通量，以及5）基于所获得的回归方程中的系数确定影响物质生产的至少一种代谢通量。

公开(公告)号：US20090148915A1

公开(公告)日：2009-06-11

申请号：US11877726

申请日：2007-10-24

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

IPC分类号： C12P13/08

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

公开(公告)号：US20060154344A1

公开(公告)日：2006-07-13

申请号：US11275437

申请日：2006-01-03

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

IPC分类号： C12P13/08 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

公开(公告)号：US20120040415A1

公开(公告)日：2012-02-16

申请号：US13170661

申请日：2011-06-28

申请人： Yuichi Nakahara ,  Yusuke Hagiwara ,  Yuri Nagai ,  Takuji Ueda ,  Kazuyuki Hayashi ,  Yuki Yano

发明人： Yuichi Nakahara ,  Yusuke Hagiwara ,  Yuri Nagai ,  Takuji Ueda ,  Kazuyuki Hayashi ,  Yuki Yano

IPC分类号： C12P13/08 ,  C12P13/04

CPC分类号： C12P13/08 ,  C12P13/04

摘要： A method is provided for producing an L-amino acid which includes the steps of culturing a bacterium, which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the culture. The culture is performed as a fed-batch culture or a continuous culture, and a feed medium containing glycerol is added to the fermentation medium so that the glycerol concentration in the fermentation medium is 5 g/L or higher.

摘要翻译： 提供了一种生产L-氨基酸的方法，其包括在含有甘油作为碳源的培养基中培养属于肠杆菌科并能够产生L-氨基酸的细菌以产生和积累的步骤 培养基中的L-氨基酸，并从培养物中收集L-氨基酸。 作为补料分批培养或连续培养进行培养，向发酵培养基中添加含有甘油的饲料培养基，发酵培养基中的甘油浓度为5g / L以上。

公开(公告)号：US08293505B2

公开(公告)日：2012-10-23

申请号：US12258630

申请日：2008-10-27

申请人： Yoko Asakura ,  Yuri Nagai ,  Takuji Ueda

发明人： Yoko Asakura ,  Yuri Nagai ,  Takuji Ueda

IPC分类号： C12Q1/10 ,  C12P21/06 ,  C12P13/04

CPC分类号： C12N9/1077 ,  C12P13/04 ,  C12P13/08 ,  C12P13/14

摘要： An L-amino acid is produced by culturing a microorganism which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, wherein the bacterium has been modified to enhance orotate phosphoribosyltransferase activity is enhanced, in a medium to produce and cause accumulation of an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过培养属于肠杆菌科的微生物并且能够产生L-氨基酸产生L-氨基酸，其中所述细菌已经被修饰以增强乳酸磷酸核糖基转移酶活性，在培养基中产生并引起积累 的介质或细胞中的L-氨基酸，并从培养基或细胞中收集L-氨基酸。