公开(公告)号：US08512987B2

公开(公告)日：2013-08-20

申请号：US12545966

申请日：2009-08-24

申请人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/24

CPC分类号： C12P13/08 ,  C12P13/04

摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

公开(公告)号：US20100221792A1

公开(公告)日：2010-09-02

申请号：US12749932

申请日：2010-03-30

申请人： YURI NAGAI ,  YOSHIHIRO USUDA

发明人： YURI NAGAI ,  YOSHIHIRO USUDA

IPC分类号： C12P13/04 ,  C12P13/08 ,  C12P13/14 ,  C12P13/24 ,  C12P13/06 ,  C12P13/22 ,  C12P13/12

CPC分类号： C12N1/20 ,  C12N9/22 ,  C12P13/08

摘要： An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which is able to produce the L-amino acid, and is modified so that the activity of ribonuclease G is decreased in a medium containing glycerol as the carbon source, and collecting the L-amino acid from the culture.

摘要翻译： 通过培养属于能够产生L-氨基酸的肠杆菌科的细菌产生L-氨基酸，并进行修饰，使得在含有作为碳源的甘油的培养基中核糖核酸酶G的活性降低， 并从培养物中收集L-氨基酸。

公开(公告)号：US08137938B2

公开(公告)日：2012-03-20

申请号：US12749932

申请日：2010-03-30

申请人： Yuri Nagai ,  Yoshihiro Usuda

发明人： Yuri Nagai ,  Yoshihiro Usuda

IPC分类号： C12P13/04 ,  C12P13/24 ,  C12P13/22

CPC分类号： C12N1/20 ,  C12N9/22 ,  C12P13/08

摘要： An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which is able to produce the L-amino acid, and is modified so that the activity of ribonuclease G is decreased in a medium containing glycerol as the carbon source, and collecting the L-amino acid from the culture.

摘要翻译： 通过培养属于能够产生L-氨基酸的肠杆菌科的细菌产生L-氨基酸，并进行修饰，使得在含有作为碳源的甘油的培养基中核糖核酸酶G的活性降低， 并从培养物中收集L-氨基酸。

公开(公告)号：US20120040415A1

公开(公告)日：2012-02-16

申请号：US13170661

申请日：2011-06-28

申请人： Yuichi Nakahara ,  Yusuke Hagiwara ,  Yuri Nagai ,  Takuji Ueda ,  Kazuyuki Hayashi ,  Yuki Yano

发明人： Yuichi Nakahara ,  Yusuke Hagiwara ,  Yuri Nagai ,  Takuji Ueda ,  Kazuyuki Hayashi ,  Yuki Yano

IPC分类号： C12P13/08 ,  C12P13/04

CPC分类号： C12P13/08 ,  C12P13/04

摘要： A method is provided for producing an L-amino acid which includes the steps of culturing a bacterium, which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the culture. The culture is performed as a fed-batch culture or a continuous culture, and a feed medium containing glycerol is added to the fermentation medium so that the glycerol concentration in the fermentation medium is 5 g/L or higher.

摘要翻译： 提供了一种生产L-氨基酸的方法，其包括在含有甘油作为碳源的培养基中培养属于肠杆菌科并能够产生L-氨基酸的细菌以产生和积累的步骤 培养基中的L-氨基酸，并从培养物中收集L-氨基酸。 作为补料分批培养或连续培养进行培养，向发酵培养基中添加含有甘油的饲料培养基，发酵培养基中的甘油浓度为5g / L以上。

公开(公告)号：US08293505B2

公开(公告)日：2012-10-23

申请号：US12258630

申请日：2008-10-27

申请人： Yoko Asakura ,  Yuri Nagai ,  Takuji Ueda

发明人： Yoko Asakura ,  Yuri Nagai ,  Takuji Ueda

IPC分类号： C12Q1/10 ,  C12P21/06 ,  C12P13/04

CPC分类号： C12N9/1077 ,  C12P13/04 ,  C12P13/08 ,  C12P13/14

摘要： An L-amino acid is produced by culturing a microorganism which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, wherein the bacterium has been modified to enhance orotate phosphoribosyltransferase activity is enhanced, in a medium to produce and cause accumulation of an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过培养属于肠杆菌科的微生物并且能够产生L-氨基酸产生L-氨基酸，其中所述细菌已经被修饰以增强乳酸磷酸核糖基转移酶活性，在培养基中产生并引起积累 的介质或细胞中的L-氨基酸，并从培养基或细胞中收集L-氨基酸。

公开(公告)号：US20100047878A1

公开(公告)日：2010-02-25

申请号：US12545966

申请日：2009-08-24

申请人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/24 ,  C12P13/04 ,  C12P13/14 ,  C12P13/08 ,  C12P13/06 ,  C12P13/22 ,  C12P13/12 ,  C12P13/10 ,  C12P13/20

CPC分类号： C12P13/08 ,  C12P13/04

摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

公开(公告)号：US20090104667A1

公开(公告)日：2009-04-23

申请号：US12258630

申请日：2008-10-27

申请人： Yoko Asakura ,  Yuri Nagai ,  Takuji Ueda

发明人： Yoko Asakura ,  Yuri Nagai ,  Takuji Ueda

IPC分类号： C12P13/04 ,  C12N1/20 ,  C12P13/08 ,  C12P13/24 ,  C12P13/06 ,  C12P13/22 ,  C12P13/12

CPC分类号： C12N9/1077 ,  C12P13/04 ,  C12P13/08 ,  C12P13/14

摘要： An L-amino acid is produced by culturing a microorganism which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, wherein the bacterium has been modified to enhance orotate phosphoribosyltransferase activity is enhanced, in a medium to produce and cause accumulation of an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

摘要翻译： 通过培养属于肠杆菌科的微生物并且能够产生L-氨基酸产生L-氨基酸，其中所述细菌已经被修饰以增强乳酸磷酸核糖基转移酶活性，在培养基中产生并引起积累 的介质或细胞中的L-氨基酸，并从培养基或细胞中收集L-氨基酸。