公开(公告)号：US20060183160A1

公开(公告)日：2006-08-17

申请号：US11403512

申请日：2006-04-12

申请人： Ziyang Zhong ,  Barbara Cordell ,  Diana Quon ,  Yu-Wang Liu ,  Qiang Xu ,  Frauke Schimmoller ,  Paul Hyslop ,  Edward Johnstone ,  Sheila Little ,  Stephen Queener ,  Tinggui Yin

发明人： Ziyang Zhong ,  Barbara Cordell ,  Diana Quon ,  Yu-Wang Liu ,  Qiang Xu ,  Frauke Schimmoller ,  Paul Hyslop ,  Edward Johnstone ,  Sheila Little ,  Stephen Queener ,  Tinggui Yin

IPC分类号： G01N33/53

CPC分类号： C12N9/6478 ,  A61K38/00 ,  C07K14/4711 ,  C12N9/6421 ,  C12Q1/37 ,  G01N33/6896 ,  G01N2500/00

摘要： The invention concerns a novel β-secretase, a method of partially purifying this novel β-secretase, and its use in assays to screen for potential drug candidates against Alzheimer's disease and other neurological diseases. The novel β-secretase has an estimated molecular weight of about 32-39 kDa or 22-26 kDa in HEK293 cell membrane extracts and human brain samples, respectively, as calculated from radiation inactivation analysis, and has a pH optimum at about pH 6.5-7.0.

摘要翻译： 本发明涉及一种新颖的β-分泌酶，一种部分纯化该新型β-分泌酶的方法，以及其用于筛选潜在的候选药物对抗阿尔茨海默病和其他神经疾病的测定中的应用。 根据放射灭活分析计算，新型β-分泌酶分别在HEK293细胞膜提取物和人脑样品中具有约32-39kDa或22-26kDa的估计分子量，并且具有约pH6.5〜 7.0。

公开(公告)号：US20070224656A1

公开(公告)日：2007-09-27

申请号：US11712211

申请日：2007-02-27

申请人： Barbara Cordell ,  Frauke Schimmoller ,  Yu-Wang Liu ,  Diana Quon

发明人： Barbara Cordell ,  Frauke Schimmoller ,  Yu-Wang Liu ,  Diana Quon

IPC分类号： C12Q1/37

CPC分类号： C12Q1/37 ,  C07K14/4711 ,  C12N9/6421 ,  G01N2500/04

摘要： The present invention is based on the findings that BACE2, a homolog of β-secretase BACE, is able to stimulate processing of APP in a non-amyloidogenic pathway, thereby suppressing the level of Aβ. Accordingly, the present invention provides methods and means for the identification and use of modulators of this unique activity of BACE2 to suppress Aβ production. The compounds identified using the methods and means provided herein may be used as potential candidates for the treatment of Alzheimer's disease and other neurological diseases.

摘要翻译： 本发明基于以下发现：BACE2是β-分泌酶BACE的同源物，能够刺激非淀粉样蛋白生成途径中APP的加工，从而抑制Abeta的水平。 因此，本发明提供了鉴定和使用BACE2独特活性调节剂来抑制Abeta生产的方法和手段。 使用本文提供的方法和手段鉴定的化合物可以用作治疗阿尔茨海默病和其他神经疾病的潜在候选物。