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公开(公告)号：US20060183160A1
公开(公告)日：2006-08-17
申请号：US11403512
申请日：2006-04-12
申请人： Ziyang Zhong , Barbara Cordell , Diana Quon , Yu-Wang Liu , Qiang Xu , Frauke Schimmoller , Paul Hyslop , Edward Johnstone , Sheila Little , Stephen Queener , Tinggui Yin
发明人： Ziyang Zhong , Barbara Cordell , Diana Quon , Yu-Wang Liu , Qiang Xu , Frauke Schimmoller , Paul Hyslop , Edward Johnstone , Sheila Little , Stephen Queener , Tinggui Yin
IPC分类号： G01N33/53
CPC分类号： C12N9/6478 , A61K38/00 , C07K14/4711 , C12N9/6421 , C12Q1/37 , G01N33/6896 , G01N2500/00
摘要： The invention concerns a novel β-secretase, a method of partially purifying this novel β-secretase, and its use in assays to screen for potential drug candidates against Alzheimer's disease and other neurological diseases. The novel β-secretase has an estimated molecular weight of about 32-39 kDa or 22-26 kDa in HEK293 cell membrane extracts and human brain samples, respectively, as calculated from radiation inactivation analysis, and has a pH optimum at about pH 6.5-7.0.
摘要翻译： 本发明涉及一种新颖的β-分泌酶，一种部分纯化该新型β-分泌酶的方法，以及其用于筛选潜在的候选药物对抗阿尔茨海默病和其他神经疾病的测定中的应用。 根据放射灭活分析计算，新型β-分泌酶分别在HEK293细胞膜提取物和人脑样品中具有约32-39kDa或22-26kDa的估计分子量，并且具有约pH6.5〜 7.0。
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公开(公告)号：US07087399B1
公开(公告)日：2006-08-08
申请号：US09566746
申请日：2000-05-09
申请人： Ziyang Zhong , Barbara Cordell , Diana Hom Quon , Yu-Wang Liu , Qiang Xu , Frauke Schimmöller , Paul Andrew Hyslop , Edward Marion Johnstone , Sheila Parks Little , Steven Wyatt Queener , Tinggui Yin
发明人： Ziyang Zhong , Barbara Cordell , Diana Hom Quon , Yu-Wang Liu , Qiang Xu , Frauke Schimmöller , Paul Andrew Hyslop , Edward Marion Johnstone , Sheila Parks Little , Steven Wyatt Queener , Tinggui Yin
CPC分类号： C12N9/6478 , A61K38/00 , C07K14/4711 , C12N9/6421 , C12Q1/37 , G01N33/6896 , G01N2500/00
摘要： The invention concerns a novel β-secretase, a method of partially purifying this novel β-secretase, and its use in assays to screen for potential drug candidates against Alzheimer's disease and other neurological diseases. The novel β-secretase has an estimated molecular weight of about 32-39 kDa or 22-26 kDa in HEK293 cell membrane extracts and human brain samples, respectively, as calculated from radiation inactivation analysis, and has a pH optimum at about pH 6.5-7.0.
摘要翻译： 本发明涉及一种新颖的β-分泌酶，一种部分纯化该新型β-分泌酶的方法，以及其用于筛选潜在的候选药物对抗阿尔茨海默病和其他神经疾病的测定中的应用。 根据放射灭活分析计算，新型β-分泌酶分别在HEK293细胞膜提取物和人脑样品中具有约32-39kDa或22-26kDa的估计分子量，并且具有约pH6.5〜 7.0。
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公开(公告)号：US06670182B1
公开(公告)日：2003-12-30
申请号：US09027258
申请日：1998-02-20
申请人： Rebecca Pruss , John Huggins , Guy Rautmann , Barbara Cordell , Jan Marian Scardina , Ronald P. Mischak
发明人： Rebecca Pruss , John Huggins , Guy Rautmann , Barbara Cordell , Jan Marian Scardina , Ronald P. Mischak
IPC分类号： C12N506
CPC分类号： G01N33/6896 , G01N2500/04 , G01N2500/10
摘要： The invention relates to the design, construction and use of eukaryotic cell lines useful in the identification of inhibitors of &bgr;-amyloid processing. More specifically, the invention relates to in vitro assays capable of identifying or quantifying a 4.2 kDa &bgr;-amyloid protein. The present invention also provides for DNA and protein molecules for the design, construction and use of eukaryotic cell lines useful in the identification of inhibitors of &bgr;-amyloid processing.
摘要翻译： 本发明涉及可用于鉴定β-淀粉样蛋白加工抑制剂的真核细胞系的设计，构建和用途。 更具体地，本发明涉及能够鉴定或定量4.2kDaβ-淀粉样蛋白的体外测定。 本发明还提供用于设计，构建和使用可用于鉴定β-淀粉样蛋白加工抑制剂的真核细胞系的DNA和蛋白质分子。
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公开(公告)号：US06221670B1
公开(公告)日：2001-04-24
申请号：US08821876
申请日：1997-03-21
IPC分类号： G01N3348
CPC分类号： G01N33/6896 , G01N33/5088 , G01N2333/4709 , G01N2800/2821
摘要： The present invention provides methods that can be used to identify &bgr;-amyloid reducing compounds. The present invention is based on the use of organotypic brain slice culturing methods to simultaneously assess the toxicity and &bgr;-amyloid reducing activity of a test compound.
摘要翻译： 本发明提供可用于鉴定β-淀粉样蛋白还原化合物的方法。 本发明基于使用器官型脑切片培养方法来同时评估测试化合物的毒性和β-淀粉样蛋白降低活性。
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公开(公告)号：US5220013A
公开(公告)日：1993-06-15
申请号：US444118
申请日：1989-11-30
申请人： Phyllis A. Ponte , Barbara Cordell
发明人： Phyllis A. Ponte , Barbara Cordell
CPC分类号： C12N15/86 , C07K14/4711 , C12N15/71 , C12Q1/6883 , G01N33/6896 , A61K2123/00 , A61K38/00 , C12N2710/24143 , C12Q2600/156 , C12Q2600/158 , G01N2800/2821
摘要： DNA sequences encoding the beta-amyloid core protein, and beta-amyloid-related proteins associated with Alzheimer's disease are disclosed. These sequences are used in producing or constructing recombinant beta-amyloid core protein, beta-amyloid-related proteins and recombinant or synthetic immunogenic peptides. These sequences are also used to identify genomic mutations and/or restriction site alterations which are associated with a predisposition to Alzheimer's disease, for purposes of genetic screening. Antibodies generated against the recombinant proteins or immunogenic peptides derived therefrom can be used for cerebral fluid or serum protein diagnosis of Alzheimer's disease.
摘要翻译： 公开了编码β-淀粉样蛋白核心蛋白的DNA序列和与阿尔茨海默病相关的β-淀粉样蛋白相关蛋白。 这些序列用于生产或构建重组β-淀粉样蛋白核心蛋白，β-淀粉样蛋白相关蛋白和重组或合成的免疫原性肽。 为了遗传筛选的目的，这些序列还用于鉴定与阿尔茨海默氏病倾向相关的基因组突变和/或限制性位点改变。 针对重组蛋白产生的抗体或由其衍生的免疫原性肽可用于脑血液或阿尔茨海默病的血清蛋白诊断。
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公开(公告)号：US20080039461A1
公开(公告)日：2008-02-14
申请号：US11648226
申请日：2006-12-28
申请人： Andrew Protter , Camilla Svensson , Tony Yaksh , Barbara Cordell , Sundeep Dugar
发明人： Andrew Protter , Camilla Svensson , Tony Yaksh , Barbara Cordell , Sundeep Dugar
IPC分类号： A61K31/535 , A61K31/415 , A61K31/445 , A61P25/00 , A61K31/505
CPC分类号： A61K31/381 , A61K31/4164 , A61K31/44 , A61K31/496
摘要： The present invention relates to methods for the prevention or treatment of pain by the inhibition of p38 MAP kinase.
摘要翻译： 本发明涉及通过抑制p38 MAP激酶预防或治疗疼痛的方法。
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公开(公告)号：US5840527A
公开(公告)日：1998-11-24
申请号：US483939
申请日：1995-06-07
IPC分类号： A61K38/00 , C07K14/785 , C12N15/70 , C12N15/85 , C12N1/00 , C12N5/10 , C12N15/12 , C12N15/63
CPC分类号： C07K14/785 , C12N15/70 , C12N15/85 , A61K38/00 , C12N2800/108 , C12N2830/002 , C12N2830/15 , C12N2830/60 , C12N2830/85
摘要： Recombinant materials for the production of low molecular weight hydrophobic lung surfactant proteins are disclosed and claimed. The monomeric forms of the proteins from human and canine sources have apparent molecular weights of about 5 kd and form dimers under some conditions. The availability of these recombinant materials permits production of large amounts of these proteins through recombinant techniques and permits the use of these proteins in pharmaceutical compositions in the treatment of respiratory deficiency syndromes.
摘要翻译： 公开和要求保护用于生产低分子量疏水性肺表面活性蛋白质的重组材料。 来自人和犬源的蛋白质的单体形式具有约5kd的表观分子量，并且在某些条件下形成二聚体。 这些重组材料的可用性允许通过重组技术生产大量的这些蛋白质，并允许在药物组合物中使用这些蛋白质来治疗呼吸缺陷综合征。
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公开(公告)号：US5430020A
公开(公告)日：1995-07-04
申请号：US74290
申请日：1993-06-09
IPC分类号： A61K38/00 , C07K14/785 , C12N15/70 , C12N15/85 , C07K7/00
CPC分类号： C07K14/785 , C12N15/70 , C12N15/85 , A61K38/00 , C12N2800/108 , C12N2830/002 , C12N2830/15 , C12N2830/55 , C12N2830/85
摘要： The complete coding sequences and amino acid sequences for both canine and human 32K alveolar surfactant proteins (ASP) are disclosed; clones encoding variants of the SP-18 and SP-5 forms of human protein are disclosed. Methods and vectors for obtaining these proteins in recombinant form are also described. An improved method for purification of the 32K protein takes advantage of its carbohydrate affinity. Pharmaceutical compositions in the treatment of respiratory deficiency syndrome use the 10K proteins with or without the 32K form. Synthetic peptides based on the human SP-5 protein are provided as well, which peptides show significant ASP activity.
摘要翻译： 公开了犬和人类32K肺泡表面活性蛋白（ASP）的完整编码序列和氨基酸序列; 公开了编码SP-18和SP-5形式的人类蛋白质的变体的克隆。 还描述了用于获得重组形式的这些蛋白质的方法和载体。 改进的32K蛋白质的纯化方法利用其碳水化合物亲合力。 治疗呼吸缺陷综合症的药物组合物使用具有或不具有32K形式的10K蛋白质。 还提供了基于人SP-5蛋白的合成肽，该肽显示出显着的ASP活性。
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9.发明授权Recombinant Alzheimer's protease inhibitory amyloid protein and method of use 失效重组阿尔茨海默病蛋白酶抑制淀粉样蛋白及其使用方法
公开(公告)号：US5223482A
公开(公告)日：1993-06-29
申请号：US361912
申请日：1989-06-06
IPC分类号： A61K38/55 , A61K38/00 , A61K49/00 , A61K51/08 , A61P1/00 , C07K1/22 , C07K14/00 , C07K14/47 , C07K14/81 , C07K16/00 , C07K16/18 , C07K16/38 , C12N9/99 , C12N15/09 , C12N15/12 , C12N15/71 , C12N15/863 , C12P21/02 , C12P21/08 , C12Q1/68 , C12R1/19 , C12R1/91 , G01N33/50 , G01N33/53 , G01N33/68
CPC分类号： C12N15/86 , C07K14/4711 , C07K14/8114 , C07K16/18 , C07K16/38 , C12N15/71 , C12Q1/6883 , G01N33/6896 , A61K2123/00 , A61K38/00 , C12N2710/24143 , C12Q2600/158 , G01N2800/2821 , Y10S435/91 , Y10S530/839
摘要： DNA sequences encoding .beta.-amyloid-related proteins associated with Alzheimer's disease are disclosed. Also provided herein is a DNA sequence encoding a novel protease inhibitor. These sequences are used in producing or constructing recombinant .beta.-amyloid core protein, .beta.-amyloid-related proteins and recombinant or synthetic immunogenic peptides. Antibodies generated against the recombinant proteins or immunogenic peptides derived therefrom can be used for cerebral fluid or serum protein diagnosis of Alzheimer's disease.
摘要翻译： 公开了编码与阿尔茨海默病相关的β-淀粉样蛋白相关蛋白的DNA序列。 本文还提供了编码新型蛋白酶抑制剂的DNA序列。 这些序列用于生产或构建重组β-淀粉样蛋白核心蛋白，β-淀粉样蛋白相关蛋白和重组或合成的免疫原性肽。 针对重组蛋白产生的抗体或由其衍生的免疫原性肽可用于脑血液或阿尔茨海默病的血清蛋白诊断。
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公开(公告)号：US4920213A
公开(公告)日：1990-04-24
申请号：US888250
申请日：1986-07-21
申请人： Beverly Dale , Barbara Cordell
发明人： Beverly Dale , Barbara Cordell
IPC分类号： A61K39/145 , C07K14/11
CPC分类号： C07K14/005 , A61K39/12 , A61K39/145 , A61K2039/5256 , A61K2039/552 , A61K2039/55566 , A61K2039/6081 , C12N2710/24143 , C12N2760/16122 , C12N2760/16134
摘要： Recombinant vaccines for immunizing horses against equine influenza virus (EIV) are disclosed. The DNA sequences encoding the hemagglutinin (HA) and neuraminidase (NA) glycoproteins from the two strains of EIV currently infective in horses are used to construct vaccinia carried vaccines, to design synthetic peptides for primer and booster administration, and to permit recombinant synthesis of HA and/or NA protein based vaccines. These DNA sequences also provide probes useful for preparing similar vaccines from fresh isolates of new strains generated by genetic drift.
摘要翻译： 公开了用于针对马流感病毒（EIV）免疫马的重组疫苗。 使用编码目前在马中感染的两种EIV菌株的血凝素（HA）和神经氨酸酶（NA）糖蛋白的DNA序列来构建携带痘苗的疫苗，设计用于引物和加强给药的合成肽，并允许重组合成HA 和/或NA蛋白质疫苗。 这些DNA序列还提供了可用于从由遗传漂移产生的新菌株的新鲜分离物制备相似疫苗的探针。
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