公开(公告)号：US6137032A

公开(公告)日：2000-10-24

申请号：US390200

申请日：1999-09-07

申请人： Kuo-Joan Cheng ,  Leonard Brent Selinger ,  Jin-Hao Liu ,  Youji Hu ,  Cecil Wallace Forsberg ,  Maurice Martin Moloney

发明人： Kuo-Joan Cheng ,  Leonard Brent Selinger ,  Jin-Hao Liu ,  Youji Hu ,  Cecil Wallace Forsberg ,  Maurice Martin Moloney

IPC分类号： C07K14/415 ,  C12N9/24 ,  C12N15/56 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12Y302/01032 ,  C07K14/415 ,  C12N15/8242 ,  C12N15/8257 ,  C12N9/2485 ,  C07K2319/00

摘要： A xylanase gene, denoted xynC, encoding a novel xylanase (XynC) obtained from the anaerobic fungus Neocallimastix patriciarum is provided. The DNA sequence of the xynC gene is also provided. Transformation of microbial and plant hosts with the xynC gene is described. The xynC gene may be used to design probes for use in hybridization experiments to isolate xylanase genes from other anaerobic fungi. The xynC gene has been used to construct an oleosin-xynC expression construct encoding an oleosin-xylanase fusion protein which retains xylanase activity. Transgenic Brassica napus (canola), transformed with the oleosin-xynC expression construct, expresses the oleosin-xylanase fusion protein which is immobilized in the oil-body membrane of the B. napus seeds. Canola meal, the protein-rich residue left after canola oil is extracted from canola plants, when derived from the transgenic B. napus of the present invention, retains substantial xylanase activity, making it an ideal animal feed supplement.

摘要翻译： 提供了一种称为xynC的木聚糖酶基因，其编码从厌氧真菌Neocallimastix patriciar获得的新型木聚糖酶（XynC）。 还提供了xynC基因的DNA序列。 描述了具有xynC基因的微生物和植物宿主的转化。 xynC基因可用于设计用于杂交实验的探针，以从其他厌氧真菌中分离木聚糖酶基因。 已经使用xynC基因构建编码保留木聚糖酶活性的油质蛋白 - 木聚糖酶融合蛋白的油质蛋白-xynC表达构建体。 用油质蛋白-xynC表达构建体转化的转基因油菜（canola）表达固定在欧洲油菜种子的油体膜中的油质蛋白 - 木聚糖酶融合蛋白。 当从本发明的转基因甘蓝型油菜中得到的油菜籽油中，从卡诺拉植物中提取出低芥酸菜籽油后留下的富含蛋白质的残留物，保留了大量的木聚糖酶活性，使其成为理想的动物饲料添加剂。

公开(公告)号：US5720971A

公开(公告)日：1998-02-24

申请号：US497913

申请日：1995-07-05

申请人： Karen A. Beauchemin ,  Lyle Rode ,  Vincent J. Sewalt

发明人： Karen A. Beauchemin ,  Lyle Rode ,  Vincent J. Sewalt

IPC分类号： A23K1/14 ,  A23K1/165 ,  A23K1/18 ,  A23K3/03

CPC分类号： C12Y302/01004 ,  A23K20/189 ,  A23K30/18 ,  A23K50/10 ,  C12Y302/01032

摘要： Fibrolytic enzyme supplements for increasing the digestibility of legume forages and grain feeds for ruminants, a method of treating legume forages and grain feeds with fibrolytic enzymes, and feed compositions consisting of feed materials treated with a mixture of fibrolytic enzymes are provided. The enzyme supplements do not pre-digest the feed material but assist in the colonization of feed particles in the rumen by ruminal microbes. The fibrolytic enzyme supplements consist of mixtures of cellulase and xylanase in certain preferred ratios and levels which are dependent on the feed material to be treated. The cellulase and xylanase are dissolved in a buffer solution and sprayed onto dry legume forages or grain feeds. The feed material is then incubated for at least three hours to allow the enzymes to be absorbed into and adhere to the feed material. The resulting feed compositions remain stable for at least one year against predigestion. When cellulase and xylanase are applied to legume forages and grain feeds in certain ratios, levels and in accordance with the methods of the present invention, synergistic effects between the enzymes occur, providing large improvements in digestibility of feed materials at low enzyme levels.

摘要翻译： 提供用于增加反刍动物的豆类饲料和谷物饲料的消化率的纤维素酶补充剂，用纤维蛋白酶处理豆类饲料和谷物饲料的方法，以及由用纤维素酶混合物处理的饲料原料组成的饲料组合物。 酶补充剂不预消化饲料，但有助于瘤胃微生物对瘤胃饲料颗粒的定植。 纤维素酶补充剂由纤维素酶和木聚糖酶的混合物组成，其依赖于待处理饲料的某些优选比例和水平。 将纤维素酶和木聚糖酶溶解在缓冲溶液中并喷洒到干燥的豆类饲料或谷物饲料上。 然后将饲料料培养至少3小时，以使酶被吸收并粘附到饲料上。 所得饲料组合物在预消化过程中保持稳定至少一年。 当纤维素酶和木聚糖酶以一定的比例，水平应用于豆类饲料和谷物进料时，根据本发明的方法，发生酶之间的协同效应，在低酶水平下提供饲料物质的消化率的大的改进。

公开(公告)号：US5728675A

公开(公告)日：1998-03-17

申请号：US465324

申请日：1995-06-05

申请人： Allan L. Schaefer ,  Stephen D. Morgan Jones ,  Richard W. Stanley ,  Ian K. S. Turnbull ,  John R. Johanns

发明人： Allan L. Schaefer ,  Stephen D. Morgan Jones ,  Richard W. Stanley ,  Ian K. S. Turnbull ,  John R. Johanns

IPC分类号： A23K1/16 ,  A23K1/175 ,  A61K38/00 ,  A61K31/195 ,  A61K31/34 ,  A61K31/355 ,  A61K31/70

CPC分类号： A23K20/24 ,  A23K20/142 ,  A23K20/147 ,  A23K20/22

摘要： The invention relates to an antemortem stress supplement for livestock containing one or more sources of electrolytes including sodium, potassium and magnesium, one or more sources of amino acids including alanine, lysine, phenylalanine, methionine, threonine, leucine, isoleucine, valine, and glutamate, all in a bypass form; and a source of tryptophan. The invention also extends to methods of administering the supplements to livestock to prevent or reduce antemortem stress

摘要翻译： 本发明涉及含有一种或多种包括钠，钾和镁的电解质来源的一种或多种包括丙氨酸，赖氨酸，苯丙氨酸，甲硫氨酸，苏氨酸，亮氨酸，异亮氨酸，缬氨酸和谷氨酸的一种或多种氨基酸来源的家畜的抗em应激补充剂 ，都是绕行的形式; 和色氨酸的来源。 本发明还延伸到给牲畜补充剂的方法以预防或减少抗堕胎压力

公开(公告)号：US5595444A

公开(公告)日：1997-01-21

申请号：US543752

申请日：1995-10-16

申请人： Alan K. Tong ,  Stephen D. M. Jones ,  Allan L. Schaefer

发明人： Alan K. Tong ,  Stephen D. M. Jones ,  Allan L. Schaefer

IPC分类号： A01K29/00 ,  A22B5/00 ,  G01N25/72 ,  G01N33/12 ,  G01N25/00

CPC分类号： A22B5/007 ,  A01K29/00 ,  G01N25/72 ,  G01N33/12

摘要： The invention provides methods of detecting poor meat quality in live animals using infrared thermography. Animals from a group of live domestic animals such as cattle or swine are scanned to produce thermographic images. The images are then statistically analyzed to determine a measure of central tendency such as the mean temperature for each animal's image and for all of the images in the group. A measure of dispersion from the measure of central tendency, such as standard deviation is determined. Then, animals are rejected as having a high probability of producing poor meat quality if the measure of central tendency for that animal's temperature differs from the measure of central tendency for the group by more than 0.9 standard deviations. Alternatively a set percent of animals are rejected, preferably up to 20%, these being animals whose measures of central tendency differ the most from the measure of central tendency for the group. When mean temperature is used as a measure of central tendency, the method is preferably practised by rejecting animals whose mean temperature differs from the group mean temperature by more than 1.28 times the standard deviation for the group. The method is particularly useful in detecting high probability of poor meat quality in groups of animals in an antemortem environment which have mean temperatures significantly above or below the normal surface temperatures for unstressed animals.

摘要翻译： 本发明提供使用红外热成像检测活体动物肉质差的方法。 来自一组活的家畜如牛或猪的动物被扫描以产生热成像图像。 然后对图像进行统计分析，以确定中心趋势的度量，例如每个动物图像的平均温度和组中所有图像的平均温度。 确定中心趋势测量的度量，如标准偏差。 然后，如果动物温度的中心倾向度量与集团集中趋势的测量不符合0.9个以上的标准偏差，那么动物就被排除在产生较差肉质的概率很高的地方。 或者，一定百分比的动物被拒绝，优选高达20％，这些动物的中心趋势的度量与组的中心趋势的度量最大。 当使用平均温度作为中心倾向的度量时，该方法优选通过排除其平均温度不同于组平均温度的动物超过该组的标准偏差的1.28倍的动物来实施。 该方法特别可用于检测在具有显着高于或低于未受胁迫动物的正常表面温度的平均温度的抗ort环境中的动物群体中较差肉质量的可能性。