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    DEVICE FOR PROVIDING A CELL SUSPENSION
    1.
    发明公开
    DEVICE FOR PROVIDING A CELL SUSPENSION 审中-公开

    公开(公告)号：US20230340386A1

    公开(公告)日：2023-10-26

    申请号：US18026664

    申请日：2021-09-21

    申请人： Lonza Cologne GmbH

    发明人： Timo GLEISSNER ,  Claudiu TODOR

    IPC分类号： C12M1/00

    CPC分类号： C12M23/38 ,  C12M29/20 ,  C12M23/34 ,  C12M29/00

    摘要： The invention relates to a device for providing a cell suspension to an apparatus for treating the cells, wherein the device comprises at least one internal space 16 for holding the suspension, and wherein the internal space 16 is at least partially surrounded by a wall 15. The device further comprises at least one inlet port 2 comprising at least one first opening 3 for introducing the suspension into the internal space 16 and at least one outlet port comprising at least one second opening for removing the suspension from said internal space 16. The inlet port 2 further comprises at least one barrier element 6 bordering the first opening 3 at a side furthest to the wall 15 and is partially arranged between the first opening 3 and the internal space 16.

    METHOD AND ELECTRODE ASSEMBLY FOR TREATING ADHERENT CELLS
    3.
    发明申请
    METHOD AND ELECTRODE ASSEMBLY FOR TREATING ADHERENT CELLS 有权
    用于治疗附着细胞的方法和电极装置

    公开(公告)号：US20130260434A1

    公开(公告)日：2013-10-03

    申请号：US13806491

    申请日：2011-06-21

    申请人： Herbert Mueller-Hartmann ,  Andreas Wirth

    发明人： Herbert Mueller-Hartmann ,  Andreas Wirth

    IPC分类号： C12N13/00

    CPC分类号： C12N13/00 ,  A61N1/327 ,  C12M23/12 ,  C12M35/02 ,  C12N15/87

    摘要： The invention relates to an electrode assembly 20, in particular for applying at least one electric field to adherent cells, comprising at least two electrodes 21, each having at least one surface 32 which is arranged opposite the corresponding surface 32 of the other electrode 21, wherein an electrically insulating material 26 is arranged at least partially between the surfaces 32 of the electrodes 21. The solution according to the invention allows the electric field to be concentrated in the region of the cells to be treated such that a voltage pulse, or the current produced thereby, flows through the cells without the majority flowing away over the cells unused in the electrolyte. The invention further relates to a method for applying at least one electric field to adherent cells, in which the electric field is generated by applying a voltage to at least two electrodes, the electric field is concentrated on the side of the electrodes which faces the cells and/or is limited to the space between the cells and the side of the electrodes which faces the cells.

    摘要翻译： 本发明涉及电极组件20，特别是用于将至少一个电场施加到贴壁电池，包括至少两个电极21，每个具有至少一个与另一电极21的对应表面32相对的表面32， 其中电绝缘材料26至少部分地布置在电极21的表面32之间。根据本发明的溶液允许电场集中在要处理的电池的区域中，使得电压脉冲或 由此产生的电流流过电池，而绝大多数电解液不会在电解质中未使用的电池上流动。 本发明还涉及一种用于将至少一个电场施加到贴壁电池的方法，其中通过向至少两个电极施加电压而产生电场，电场集中在面对电池的电极侧 和/或限于电池与面对电池的电极侧之间的空间。

    Device and method for large volume transfection
    8.
    发明授权
    Device and method for large volume transfection 有权

    公开(公告)号：US11352615B2

    公开(公告)日：2022-06-07

    申请号：US16185045

    申请日：2018-11-09

    申请人： Lonza Cologne GmbH

    发明人： Ludger Altrogge ,  Timo Gleissner ,  Andreas Heinze ,  Sven Hermsmeier

    IPC分类号： C12N13/00 ,  C12M3/00 ,  C12M1/42 ,  C12N15/87 ,  A61N1/04

    摘要： The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6). According to the invention each compartment (26, 27) is designed to hold the suspension and comprises at least one port (7, 8, 10, 11) for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber (6) and at the same time charge a further aliquot of the suspension into the chamber (6), wherein the separating element (13) is moved in a second direction opposite to a first direction, and wherein the separating element (13) separates the aliquots from each other.

    DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION
    9.
    发明申请
    DEVICE AND METHOD FOR LARGE VOLUME TRANSFECTION 审中-公开

    公开(公告)号：US20200325466A1

    公开(公告)日：2020-10-15

    申请号：US16820847

    申请日：2020-03-17

    申请人： Lonza Cologne GmbH

    发明人： Ludger ALTROGGE ,  Timo GLEISSNER ,  Andreas HEINZE ,  Sven HERMSMEIER

    IPC分类号： C12N13/00 ,  C12M1/42 ,  C12N15/87 ,  A61N1/04

    摘要： Disclosed is a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. Also disclosed is a method in which voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.

    METHOD AND DEVICE FOR UNIFORMLY TREATING ADHERENT CELLS
    10.
    发明申请
    METHOD AND DEVICE FOR UNIFORMLY TREATING ADHERENT CELLS 有权
    用于均匀处理附着细胞的方法和装置

    公开(公告)号：US20130230895A1

    公开(公告)日：2013-09-05

    申请号：US13806395

    申请日：2011-06-21

    申请人： Thomas Koblizek ,  Andreas Heinze ,  Timo Gleissner ,  Herbert Mueller-Hartmann ,  Andreas Wirth

    发明人： Thomas Koblizek ,  Andreas Heinze ,  Timo Gleissner ,  Herbert Mueller-Hartmann ,  Andreas Wirth

    IPC分类号： C12N13/00

    CPC分类号： C12N13/00 ,  A61N1/327 ,  C12M23/12 ,  C12M35/02 ,  C12N15/87

    摘要： The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.

    摘要翻译： 本发明涉及一种使贴壁细胞经受至少一个电场的方法，其中通过向至少两个有效电极63施加电压而产生电场，其中至少提供三个电极63,64，其中在 当施加电压以产生第一电场时，至少两个电极63,64是有效电极63，并且其中产生至少一个第二电场，其中两个先前有效电极63中的至少一个是电位 - 当施加电压时，电极64。 本发明还涉及包括至少三个电极63,64的装置58，这些电极通过至少一个开关装置59,60,61,62连接到至少一个电压源，其中至少五个电极63,64 通过四个开关装置59,60,61,62连接到至少一个电压源，其中至少两个电极63,64通过公共开关装置59,60,61连接到电压源， 结合上述方法，根据本发明的装置允许使用电场对粘附细胞进行有效和均匀的处理，其中有效和无电极之间的开关可以使用较低的 开关器件数量。