公开(公告)号：US10889846B2

公开(公告)日：2021-01-12

申请号：US15580582

申请日：2016-06-03

Applicant: Sarissa Biomedical Limited

Inventor： Nicholas Dale ,  Chris Imray ,  Bruno Frenguelli

IPC: G01N27/327 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/543 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/48

Abstract: The invention provides a method of determining a predisposition to a condition associated with ATP depletion, such as an ischaemic event, in a subject comprising: a. measuring the concentration of one or more purines in a body fluid of the subject, the purines being selected from adenosine, inosine, hypoxanthine, xanthine and ATP, and b. comparing the measured concentration with a threshold concentration of the one or more purines, wherein the threshold concentration is preferably in the range 2 [micro]M to 8 [micro]M and wherein a measured concentration higher than the threshold concentration indicates the presence of ischaemia.

公开(公告)号：US10907189B2

公开(公告)日：2021-02-02

申请号：US15580575

申请日：2016-06-03

Applicant: Sarissa Biomedical Limited

Inventor： Nicholas Dale ,  Chris Imray ,  Faming Tian

IPC: G01N27/327 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/543

Abstract: The invention describes a method of determining the presence of acute cerebral ischaemic disorder in a subject comprising: a. measuring the concentration of one or more purines in a body fluid of the subject, the purines being selected from adenosine, inosine, hypoxanthine, xanthine and ATP, and b. comparing the measured concentration with a threshold concentration of the one or more purines, wherein the threshold concentration is preferably in the range 5 μM to 15 μM and wherein a measured concentration higher than the threshold concentration indicates the presence of acute ischaemic disorder. Also is described a method of determining the absence of an acute cerebral disorder in a subject comprising: a. measuring the concentration of one or more purines in a body fluid of the subject, the purines being selected from adenosine, inosine, hypoxanthine, xanthine and ATP, and b. comparing the measured concentration with a threshold concentration of the one or more purines, wherein the threshold concentration is preferably below 4 μM and wherein a measured concentration lower than the threshold concentration indicates the absence of acute cerebral ischaemic disorder.

公开(公告)号：US09791401B2

公开(公告)日：2017-10-17

申请号：US14649993

申请日：2013-11-28

Applicant: SARISSA BIOMEDICAL LIMITED

Inventor： Nicholas Dale ,  Faming Tian

IPC: G01N27/327 ,  A61B10/00 ,  G01N33/543 ,  C12Q1/00 ,  B01L3/00

CPC classification number: G01N27/327 ,  A61B10/0096 ,  B01L3/5021 ,  B01L3/50825 ,  B01L2300/046 ,  B01L2300/0636 ,  B01L2300/0645 ,  C12Q1/003 ,  G01N27/3271 ,  G01N33/5438 ,  G01N2223/307 ,  G01N2800/2871 ,  G01N2800/302

Abstract: A device utilizing biosensors to enable rapid electrochemical sensing of one or more analytes in a container. The device comprises a holder which incorporates at least one reference electrode and at least one sensing electrode. The sensing electrode comprising an electrically conductive substrate which is coated in a first layer of a suitable electron acceptor and subsequently with a second layer incorporating a biorecognition molecule adsorbed or within a suitable electropolymer matrix or carrier.