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    Large volume ex vivo electroporation method
    2.
    发明申请
    Large volume ex vivo electroporation method 有权
    大体积体外电穿孔法

    公开(公告)号：US20060108229A1

    公开(公告)日：2006-05-25

    申请号：US10537254

    申请日：2004-03-15

    申请人： Richard Walters ,  Alan King

    发明人： Richard Walters ,  Alan King

    IPC分类号： C25D5/18

    CPC分类号： C12N13/00 ,  A61K9/0009 ,  C12M1/42 ,  C12M3/00 ,  C12M3/006 ,  C12M3/02 ,  C12M35/02 ,  C12M45/07 ,  C12N15/87

    摘要： An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm−1) defined by the quotient of the electrode gap squared (cm2) divided by the chamber volume (cm3), wherein the geometric factor is less than or equal to 0.1 cm−1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 0.01 to 1.0 milliSiemens, wherein the suspension is enclosed in the chamber during treatment, and h. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform.

    摘要翻译： 本发明的目的是提供一种用外源材料处理囊泡的电穿孔方法，用于将外源材料插入囊泡中，其包括以下步骤：a。 将囊泡和外源材料的悬浮液保持在包括电极的室中的处理体积中，其中所述室具有由电极间隙平方的商定义的几何因子（cm -1 -1 -1） cm 2）除以室容积（cm 3），其中几何因子小于或等于0.1cm -1，其中 囊泡和外源材料的悬浮液在调节介质中使介质具有在0.01至1.0毫西门子范围内的电导率的介质，其中悬浮液在处理过程中封闭在腔室中，而h。 用一个或多个脉冲电场处理封闭在腔室中的悬架。 利用该方法，悬浮液的处理体积是可扩展的，并且腔室中的囊泡的处理时间基本上是均匀的。

    Large volume ex vivo electroporation method
    4.
    发明授权
    Large volume ex vivo electroporation method 有权

    公开(公告)号：US09982251B2

    公开(公告)日：2018-05-29

    申请号：US10537254

    申请日：2004-03-15

    申请人： Richard E. Walters ,  Alan D. King

    发明人： Richard E. Walters ,  Alan D. King

    IPC分类号： C12N13/00 ,  C12N15/87 ,  C12M3/02 ,  C12M3/00 ,  C12M1/42 ,  C12M1/00 ,  A61K9/00

    CPC分类号： C12N13/00 ,  A61K9/0009 ,  C12M1/42 ,  C12M3/00 ,  C12M3/006 ,  C12M3/02 ,  C12M35/02 ,  C12M45/07 ,  C12N15/87

    摘要： An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm.sup.−1) defined by the quotient of the electrode gap squared (cm.sup.2) divided by the chamber volume (cm.sup.3), wherein the geometric factor is less than or equal to 0.1 cm.sup.−1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 50 microSiemens/cm to 500 microSiemens/cm, wherein the suspension is enclosed in the chamber during treatment, and b. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform.

    Large Volume Ex Vivo Electroporation Method
    5.
    发明申请
    Large Volume Ex Vivo Electroporation Method 审中-公开

    公开(公告)号：US20180237765A1

    公开(公告)日：2018-08-23

    申请号：US15956103

    申请日：2018-04-18

    申请人： Cellectis S.A.

    发明人： Richard E Walters ,  Alan D King

    IPC分类号： C12N13/00

    CPC分类号： C12N13/00 ,  A61K9/0009 ,  C12M1/42 ,  C12M3/00 ,  C12M3/006 ,  C12M3/02 ,  C12M35/02 ,  C12M45/07 ,  C12N15/87 ,  H02K1/146 ,  H02K1/246 ,  H02K19/103

    摘要： An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm−1 ) defined by the quotient of the electrode gap squared (cm2) divided by the chamber volume (cm3), wherein the geometric factor is less than or equal to 0.1 cm−1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 50 microSiemens/cm to 500 microSiemens/cm, wherein the suspension is enclosed in the chamber during treatment, and b. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform.

    Apparatus and method that prevent flux reversal in the stator back material of a two-phase SRM (TPSRM)
    6.
    发明授权
    Apparatus and method that prevent flux reversal in the stator back material of a two-phase SRM (TPSRM) 有权
    防止二相SRM（TPSRM）定子背材料中的磁通反转的装置和方法

    公开(公告)号：US07579734B2

    公开(公告)日：2009-08-25

    申请号：US10549800

    申请日：2004-03-17

    申请人： Krishnan Ramu ,  Savio Nimal Lobo

    发明人： Krishnan Ramu ,  Savio Nimal Lobo

    IPC分类号： H02K17/42

    CPC分类号： C12N13/00 ,  A61K9/0009 ,  C12M1/42 ,  C12M3/00 ,  C12M3/006 ,  C12M3/02 ,  C12M35/02 ,  C12M45/07 ,  C12N15/87 ,  H02K1/146 ,  H02K1/246 ,  H02K19/103

    摘要： A TPSRM may include a stator, having a plurality of poles and a ferromagnetic or iron back material, and a rotor having a plurality of poles and a ferromagnetic or iron back material. A current flowing through coils wound around a first set of the plurality of stator poles induces a flux flow through the first set of stator poles and portions of the stator back material during a first excitation phase. A current flowing through coils wound around a second set of the plurality of stator poles induces a flux flow through the second set of stator poles and portions of the stator back material during a second excitation phase. The numbers of stator and rotor poles for this TPSRM are selected such that substantially no flux reversal occurs in any part of the stator back material as a result of transitioning between the first and second excitation phases.

    摘要翻译： TPSRM可以包括具有多个极和铁磁或铁背材料的定子，以及具有多个极和铁磁或铁背材料的转子。 在第一激励阶段期间，流过卷绕在第一组多个定子极上的线圈的电流在第一组定子极和定子背材料的部分引起磁通流。 在第二激励阶段期间，流过绕第二组定子极的线圈的电流在第二组定子极和定子背材料的一部分引起磁通流。 选择该TPSRM的定子和转子极数，使得在第一和第二激发相之间的转变的结果，基本上不会在定子背材料的任何部分发生磁通反转。

    Apparatus and method that prevent flux reversal in the stator back material of a two-phase SRM (TPSRM)
    8.
    发明申请
    Apparatus and method that prevent flux reversal in the stator back material of a two-phase SRM (TPSRM) 失效
    防止二相SRM（TPSRM）定子背材料中的磁通反转的装置和方法

    公开(公告)号：US20040263013A1

    公开(公告)日：2004-12-30

    申请号：US10802054

    申请日：2004-03-17

    发明人： Krishnan Ramu ,  Nimal Savio Lobo

    IPC分类号： H02K017/00 ,  H02K017/42

    CPC分类号： C12N13/00 ,  A61K9/0009 ,  C12M1/42 ,  C12M3/00 ,  C12M3/006 ,  C12M3/02 ,  C12M35/02 ,  C12M45/07 ,  C12N15/87 ,  H02K1/146 ,  H02K1/246 ,  H02K19/103

    摘要： A TPSRM may include a stator, having a plurality of poles and a ferromagnetic or iron back material, and a rotor having a plurality of poles and a ferromagnetic or iron back material. A current flowing through coils wound around a first set of the plurality of stator poles induces a flux flow through the first set of stator poles and portions of the stator back material during a first excitation phase. A current flowing through coils wound around a second set of the plurality of stator poles induces a flux flow through the second set of stator poles and portions of the stator back material during a second excitation phase. The numbers of stator and rotor poles for this TPSRM are selected such that substantially no flux reversal occurs in any part of the stator back material as a result of transitioning between the first and second excitation phases.

    摘要翻译： TPSRM可以包括具有多个极和铁磁或铁背材料的定子，以及具有多个极和铁磁或铁背材料的转子。 在第一激励阶段期间，流过卷绕在第一组多个定子极上的线圈的电流在第一组定子极和定子背材料的部分引起磁通流。 在第二激励阶段期间，流过绕第二组定子极的线圈的电流在第二组定子极和定子背材料的一部分引起磁通流。 选择该TPSRM的定子和转子极数，使得在第一和第二激发相之间的转变的结果，基本上不会在定子背材料的任何部分发生磁通反转。