利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

17 条记录 (耗时 0.02 秒)

    Compositions and methods for reverse transcription of nucleic acid molecules
    2.
    发明申请
    Compositions and methods for reverse transcription of nucleic acid molecules 有权
    核酸分子逆转录的组成和方法

    公开(公告)号：US20030186270A1

    公开(公告)日：2003-10-02

    申请号：US10292604

    申请日：2002-11-13

    申请人： Invitrogen Corporation

    发明人： Gary F. Gerard ,  Michael D. Smith ,  Deb K. Chatterjee

    IPC分类号： C12Q001/68 ,  C12P019/34 ,  C12N009/22

    CPC分类号： C12N15/1096 ,  C12N9/1276 ,  C12N2740/10011 ,  C12N2740/11011 ,  C12Q1/6848 ,  C12Q2527/101 ,  C12Q2521/301 ,  C12Q2521/107

    摘要： The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55null C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (null and/or null subunits thereof), to isolated nucleic acid molecules encoding such RSV RT, AMV RT or other ASLV RT subunits, to vectors and host cells comprising these isolated nucleic acid molecules and to RSV RT, AMV RT and other ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, particularly heterodimeric RSV RTs, AMV RTs or other ASLV RTs (which may be nullnull RTs, nullnull RTs, or null RTs), vectors (particularly baculovirus vectors) and host cells (particularly insect and yeast cells) comprising these nucleic acid molecules, methods for producing these heterodimeric RTs and heterodimeric RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, or RSV RTs, AMV RTs or other ASLV RTs of the invention.

    摘要翻译： 本发明通常涉及核酸分子，特别是信使RNA分子的逆转录的组合物和方法。 具体地，本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物，以及使用这些组合物或多肽，特别是在高于约55℃的温度下产生，扩增或测序核酸分子（特别是cDNA分子）的方法 本发明还涉及通过这些方法产生的核酸分子，涉及包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。 本发明还涉及用于生产分离的编码这样的核酸分子的Rous肉瘤病毒（RSV）和禽成髓细胞病毒（AMV）RT或其他禽类肉瘤 - 白血病病毒（ASLV）RT（其α和/或β亚基））的方法 RSV RT，AMV RT或其他ASLV RT亚单位，包含这些分离的核酸分子的载体和宿主细胞以及通过这些方法产生的RSV RT，AMV RT和其他ASLV RT亚基。 本发明还涉及编码重组异源二聚体RT全酶，特别是异二聚体RSV RT，AMV RT或其他ASLV RT（其可以是字母表RT，betabeta RT或αRT），载体（特别是杆状病毒载体）和宿主细胞（ 特别是昆虫和酵母细胞），通过这些方法产生的这些异源二聚体RT和异二聚体RT的制备方法。 本发明还涉及包含本发明的组合物，多肽或RSV RT，AMV RT或其他ASLV RT的试剂盒。

    Compositions and methods for reverse transcription of nucleic acid molecules
    4.
    发明授权
    Compositions and methods for reverse transcription of nucleic acid molecules 有权
    核酸分子逆转录的组成和方法

    公开(公告)号：US06518019B2

    公开(公告)日：2003-02-11

    申请号：US09245026

    申请日：1999-02-05

    申请人： Gary F. Gerard ,  Michael D. Smith ,  Deb K. Chatterjee

    发明人： Gary F. Gerard ,  Michael D. Smith ,  Deb K. Chatterjee

    IPC分类号： C12Q168

    CPC分类号： C12N15/1096 ,  C12N9/1276 ,  C12N2740/10011 ,  C12N2740/11011 ,  C12Q1/6848 ,  C12Q2527/101 ,  C12Q2521/301 ,  C12Q2521/107

    摘要： The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures about about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (&agr; and/or &bgr; subunits thereof), to isolated nucleic acid molecules encoding such RSV RT, AMV RT or other ASLV RT subunits, to vectors and host cells comprising these isolated nucleic acid molecules and to RSV RT, AMV RT and other ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, particularly heterodimeric RSV RTs, AMV RTs or other ASLV RTs (which may be &agr;&bgr; RTs, &bgr;&bgr; RTs, or &agr; RTs), vectors (particularly baculovirus vectors) and host cells (particularly insect and yeast cells) comprising these nucleic acid molecules, methods for producing these heterodimeric RTs and heterodimeric RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, or RSV RTs, AMV RTs or other ASLV RTs of the invention.

    摘要翻译： 本发明通常涉及核酸分子，特别是信使RNA分子的逆转录的组合物和方法。 具体地，本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物，以及使用这些组合物或多肽，特别是在约55℃的温度下产生，扩增或测序核酸分子（特别是cDNA分子）的方法 本发明还涉及通过这些方法产生的核酸分子，涉及包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。 本发明还涉及用于生产分离的编码这样的核酸分子的Rous肉瘤病毒（RSV）和禽成髓细胞病毒（AMV）RT或其他禽类肉瘤 - 白血病病毒（ASLV）RT（其α和/或β亚基））的方法 RSV RT，AMV RT或其他ASLV RT亚单位，包含这些分离的核酸分子的载体和宿主细胞以及通过这些方法产生的RSV RT，AMV RT和其他ASLV RT亚基。 本发明还涉及编码重组异源二聚体RT全酶，特别是异二聚体RSV RT，AMV RT或其他ASLV RT（其可以是字母表RT，betabeta RT或αRT），载体（特别是杆状病毒载体）和宿主细胞（ 特别是昆虫和酵母细胞），通过这些方法产生的这些异源二聚体RT和异二聚体RT的制备方法。 本发明还涉及包含本发明的组合物，多肽或RSV RT，AMV RT或其他ASLV RT的试剂盒。

    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTION OF NUCLEIC ACID MOLECULES
    6.
    发明申请
    COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTION OF NUCLEIC ACID MOLECULES 审中-公开
    反向转录核酸分子的组合物和方法

    公开(公告)号：US20110008770A1

    公开(公告)日：2011-01-13

    申请号：US12511569

    申请日：2009-07-29

    申请人： Gary F. Gerard ,  Michael D. Smith ,  Deb K. Chatterjee

    发明人： Gary F. Gerard ,  Michael D. Smith ,  Deb K. Chatterjee

    IPC分类号： C12Q1/68 ,  C12N9/12 ,  C12P19/34

    CPC分类号： C12N15/1096 ,  C12N9/1276 ,  C12N2740/10011 ,  C12N2740/11011 ,  C12Q1/6848 ,  C12Q2527/101 ,  C12Q2521/301 ,  C12Q2521/107

    摘要： The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (α and/or β subunits thereof), to isolated nucleic acid molecules encoding such RSV RT, AMV RT or other ASLV RT subunits, to vectors and host cells comprising these isolated nucleic acid molecules and to RSV RT, AMV RT and other ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, particularly heterodimeric RSV RTs, AMV RTs or other ASLV RTs (which may be αβ RTs, ββ RTs, or α RTs), vectors (particularly baculovirus vectors) and host cells (particularly insect and yeast cells) comprising these nucleic acid molecules, methods for producing these heterodimeric RTs and heterodimeric RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, or RSV RTs, AMV RTs or other ASLV RTs of the invention.

    摘要翻译： 本发明通常涉及核酸分子，特别是信使RNA分子的逆转录的组合物和方法。 具体地，本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物，以及使用这些组合物或多肽，特别是在高于约55℃的温度下产生，扩增或测序核酸分子（特别是cDNA分子）的方法 本发明还涉及通过这些方法产生的核酸分子，涉及包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。 本发明还涉及用于生产分离的核酸分子编码的Rous肉瘤病毒（RSV）和禽成髓细胞增殖症病毒（AMV）RT或其他禽类肉瘤 - 白血病病毒（ASLV）RT（α和/或其亚基）的方法 这样的RSV RT，AMV RT或其他ASLV RT亚基，涉及包含这些分离的核酸分子的载体和宿主细胞以及通过这些方法产生的RSV RT，AMV RT和其他ASLV RT亚基。 本发明还涉及编码重组异源二聚体RT全酶，特别是异二聚体RSV RT，AMV RT或其它ASLV RT（其可以是α和bgr; RTs，bgr; RT或RT）的载体（特别是杆状病毒载体）的核酸分子， 和包含这些核酸分子的宿主细胞（特别是昆虫和酵母细胞），通过这些方法生产这些异源二聚体RT和异二聚体RT的方法。 本发明还涉及包含本发明的组合物，多肽或RSV RT，AMV RT或其他ASLV RT的试剂盒。

    Recombinant methods for making reverse transcriptases and mutants thereof
    8.
    发明授权
    Recombinant methods for making reverse transcriptases and mutants thereof 失效
    制备逆转录酶及其突变体的重组方法

    公开(公告)号：US06989259B2

    公开(公告)日：2006-01-24

    申请号：US09064057

    申请日：1998-04-22

    申请人： Gary F. Gerard ,  Michael D. Smith ,  Deb K. Chatterjee

    发明人： Gary F. Gerard ,  Michael D. Smith ,  Deb K. Chatterjee

    IPC分类号： C12N9/12

    CPC分类号： C12N15/1096 ,  C12N9/1276 ,  C12N2740/10011 ,  C12N2740/11011 ,  C12Q1/6848 ,  C12Q2527/101 ,  C12Q2521/301 ,  C12Q2521/107

    摘要： The invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity and to methods of producing, amplifying or sequencing nucleic acid molecules using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and use of such nucleic acid molecules to produce desired polypeptide. The invention also relates to methods for producing Avian Sarcoma-Leukosis Virus (ASLV) RT subunits, in particular, Avian Myeloblastosis Virus (AMV) RTs, to isolated nucleic acid molecules encoding ASLV RT subunits, and to ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant RT holoenzymes, particularly ASLV RTs, methods for producing these RTs and to RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, and ASLV RTs of the invention.

    摘要翻译： 本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物以及使用这些组合物或多肽，特别是在高于约55℃的温度下产生，扩增或测序核酸分子的方法。本发明还涉及核酸 通过这些方法产生的分子与包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。 本发明还涉及用于将编码ASLV RT亚基的分离的核酸分子以及通过这些方法产生的ASLV RT亚基生产禽类肉瘤 - 白血病病毒（ASLV）RT亚单位，特别是禽成髓细胞病毒（AMV）RT）的方法。 本发明还涉及编码重组RT全酶，特别是ASLV RT的核酸分子，用于制备这些RT的方法和通过这些方法产生的RT。 本发明还涉及包含本发明的组合物，多肽和ASLV RT的试剂盒。