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68 条记录 (耗时 0.031 秒)

    Real time detection of intermolecular interaction
    2.
    发明授权
    Real time detection of intermolecular interaction 失效

    公开(公告)号：US06936427B2

    公开(公告)日：2005-08-30

    申请号：US10071844

    申请日：2002-02-08

    申请人： Lawrence M. Kauvar

    发明人： Lawrence M. Kauvar

    IPC分类号： C40B30/04 ,  G01N33/566 ,  G01N33/68 ,  G01N33/53

    CPC分类号： C40B30/04 ,  G01N33/566 ,  G01N33/6845 ,  G01N33/6857

    摘要： Intermolecular binding can be detected by formation of a “paratope” which results in an immediate generation of a signal. The substances to be tested for interaction are bound to demitopes, wherein said demitopes are components of a paratope which binds a reporter which provides said signal when bound. Known interactions measured in this way can also be employed to screen for compounds which interfere with the interactions. In addition to testing for individual interactions, the interaction of a compound with a library or library×library interactions can also be determined and the effect of potentially interfering substances evaluated.

    High throughput microscopy
    4.
    发明授权
    High throughput microscopy 失效
    高通量显微镜

    公开(公告)号：US06444992B1

    公开(公告)日：2002-09-03

    申请号：US09394842

    申请日：1999-09-13

    申请人： Lawrence M. Kauvar ,  John Sedat

    发明人： Lawrence M. Kauvar ,  John Sedat

    IPC分类号： G01N2164

    CPC分类号： G02B21/365 ,  G02B21/16 ,  G02B21/26

    摘要： An apparatus for high resolution, high throughput, three-dimensional fluorescence microscopy with a rotatable circular disc for mounting samples to be viewed and multiple CCDs and beam splitters as detectors for the fluorescence emission.

    摘要翻译： 一种用于高分辨率，高通量，三维荧光显微镜的装置，其具有用于安装要观察的样品的可旋转圆盘，以及用于荧光发射的检测器的多个CCD和分束器。

    Determination of concentration by affinity titration
    6.
    发明授权
    Determination of concentration by affinity titration 失效
    通过亲和滴定测定浓度

    公开(公告)号：US5620901A

    公开(公告)日：1997-04-15

    申请号：US238379

    申请日：1994-05-05

    申请人： Lawrence M. Kauvar

    发明人： Lawrence M. Kauvar

    IPC分类号： A61K47/48 ,  G01N33/53 ,  G01N33/543

    CPC分类号： A61K47/48438 ,  G01N33/53 ,  G01N33/54306 ,  Y10S435/81 ,  Y10S435/97

    摘要： A method for determining the amount of analyte in a sample utilizes a series of test regions with systematically varied, preferably monotonically increasing, affinity for a specific binding partner for an analyte or for the analyte itself. By determining the portion of test regions which are capable of binding specific binding partner in competition with the analyte in the sample, or which bind the analyte, the amount of analyte may be estimated. Thus, titration of affinity replaces titration of concentration, allowing assays to be performed without the need for serial dilution steps.

    摘要翻译： 用于确定样品中分析物的量的方法利用一系列测试区域，其对于分析物或分析物本身的特异性结合配偶体系统地改变，优选单调递增的亲和力。 通过确定能够结合特异性结合配偶体与样品中分析物竞争或测量结合分析物的部分测试区域，可以估计分析物的量。 因此，亲和力的滴定取代浓度的滴定，允许进行测定而不需要连续稀释步骤。

    Determination of concentration by affinity titration
    7.
    发明授权
    Determination of concentration by affinity titration 失效
    通过亲和滴定测定浓度

    公开(公告)号：US5356784A

    公开(公告)日：1994-10-18

    申请号：US39786

    申请日：1993-03-30

    申请人： Lawrence M. Kauvar

    发明人： Lawrence M. Kauvar

    IPC分类号： A61K47/48 ,  G01N33/53 ,  G01N33/543

    CPC分类号： A61K47/48438 ,  G01N33/53 ,  G01N33/54306 ,  Y10S435/81 ,  Y10S435/97

    摘要： A method for determining the amount of analyte in a sample utilizes a series of test regions with systematically varied, preferably monotonically increasing, affinity for a specific binding partner for an analyte or for the analyte itself. By determining the portion of test regions which are capable of binding specific binding partner in competition with the analyte in the sample, or which bind the analyte, the amount of analyte may be estimated. Thus, titration of affinity replaces titration of concentration, allowing assays to, be performed without the need for serial dilution steps.

    摘要翻译： 用于确定样品中分析物的量的方法利用一系列测试区域，其对于分析物或分析物本身的特异性结合配偶体系统地改变，优选单调递增的亲和力。 通过确定能够结合特异性结合配偶体与样品中分析物竞争或测量结合分析物的部分测试区域，可以估计分析物的量。 因此，亲和力的滴定取代浓度的滴定，允许进行测定，而不需要连续稀释步骤。

    Method to produce immunodiagnostic reagents
    8.
    发明授权
    Method to produce immunodiagnostic reagents 失效
    生产免疫诊断试剂的方法

    公开(公告)号：US5217869A

    公开(公告)日：1993-06-08

    申请号：US255906

    申请日：1988-10-11

    申请人： Lawrence M. Kauvar

    发明人： Lawrence M. Kauvar

    IPC分类号： C07K7/06 ,  C07K7/08 ,  C07K16/00 ,  C07K16/30 ,  G01N30/00 ,  G01N30/02 ,  G01N33/68

    CPC分类号： C07K7/06 ,  C07K16/00 ,  C07K16/30 ,  C07K7/08 ,  G01N33/6878 ,  C07K2317/34 ,  G01N30/00 ,  G01N30/02 ,  Y10S435/975 ,  Y10S436/809 ,  Y10S530/808 ,  Y10S530/809

    摘要： Screening methods to obtain suitable antibodies for use in immunoassays for analytes not ordinarily susceptible to detection by this means involves in vitro screening of panels of cells secreting a representative selection of antibodies. An application of this method also permits the preparation of specific mimotopes which mimic the immunological activity of the desired analyte, which mimotopes can then be used as competitors in the immunoassay or can be used to immunize subject mammals in order to improve the specificity and affinity of the antibodies. Methods to identify a particular analyte by its pattern of binding strength to a panel of related antibodies and to match an arbitrary analyte with an immunoreactive member of a panel of candidate antibodies are also disclosed.

    摘要翻译： 获得用于免疫测定中的合适抗体的筛选方法，用于通常不易于通过这种方式检测的分析物包括体外筛选分泌抗体的代表性选择的细胞组。 该方法的应用还允许制备模拟所需分析物的免疫活性的特异性模拟表位，该模拟表达可随后在免疫测定中用作竞争者，或者可用于免疫受试哺乳动物以改善受试哺乳动物的特异性和亲和力 抗体。 还公开了通过其与一组相关抗体的结合强度的模式来鉴定特定分析物并将任意分析物与一组候选抗体的免疫反应性成员相匹配的方法。