公开(公告)号：US4294927A

公开(公告)日：1981-10-13

申请号：US92554

申请日：1979-11-08

申请人： Lajos Alfoldi ,  Katalin Balint nee Fodor ,  Csaba Kari ,  Istvan Torok ,  Gyorgy Szvoboda ,  Tibor Lang ,  Istvan Gado ,  Gabor Ambrus

发明人： Lajos Alfoldi ,  Katalin Balint nee Fodor ,  Csaba Kari ,  Istvan Torok ,  Gyorgy Szvoboda ,  Tibor Lang ,  Istvan Gado ,  Gabor Ambrus

IPC分类号： C12N15/03 ,  C12N15/00

CPC分类号： C12R1/29 ,  C12N15/03 ,  C12R1/31 ,  Y10S435/867 ,  Y10S435/869

摘要： The invention provides a process for the preparation of antibiotic-producer Micromonospora strains having modified genetic material, wherein a protoplast suspension is prepared with lysozyme, under osmotically buffered conditions ensured by sucose, from each culture of the two genetically marked mutants of the antibiotic-producer Micromonospora strain following cultivation in a glycine medium, both suspensions obtained are combined in the presence of polyethylene glycol, and incubated at room temperature, the resulting fused protoplasts are suspended in soft agar, then plated on an agar plate containing sucrose, prolin and inorganic salts, incubated for 20 to 30 days, and finally all mutants are selected from the regenerated colonies which are sure to have genetic material different from that of the parent strains.The process can be advantageously applied to improve productivity of antibiotic-producer strains having industrial importance.

摘要翻译： 本发明提供了一种用于制备具有修饰遗传物质的抗生素 - 生产物单孢菌菌株的方法，其中在抗生素生产者的两个遗传标记的突变体的每个培养物中，在通过sucose保证的渗透缓冲条件下，用溶菌酶制备原生质体悬浮液 在甘氨酸培养基中培养后的微单孢菌菌株，将得到的两种悬浮液在聚乙二醇存在下合并，并在室温下温育，将得到的融合原生质体悬浮在软琼脂中，然后铺在含有蔗糖，脯氨酸和无机盐 ，孵育20至30天，最后所有突变体选自再生菌落，其确保具有与亲本菌株不同的遗传物质。 该方法可以有利地应用于提高具有工业重要性的抗生素 - 生产菌株的生产力。

公开(公告)号：US4412068A

公开(公告)日：1983-10-25

申请号：US271552

申请日：1981-06-08

申请人： David Rosi

发明人： David Rosi

IPC分类号： C07H15/236 ,  C12P19/48 ,  C07G11/00 ,  C07H15/26 ,  C12R1/31

CPC分类号： C12R1/31 ,  C07H15/236 ,  C12P19/485 ,  Y02P20/55 ,  Y10S435/869

摘要： 5-Deoxygentamicin C.sub.2b and 6'-N-methyl-2-hydroxy- and 6'-N-methyl-5-deoxygentamicins C.sub.2b are prepared, as the major fermentation products, by culturing a nutrient medium containing carbohydrates, a source of assimilable nitrogen, essential salts and either D-streptamine or 2,5-dideoxystreptamine with a mutant of Micromonospora purpurea and isolating the fermentation products from the nutrient medium. The fermentation products are acylated with an ester of an .omega.-[N-(benzyloxycarbonyl)amino]-.alpha.-hydroxy-lower-alkanoic acid after first blocking the primary amino group at the 2'-position with an amine-protecting group, followed by catalytic hydrogenolysis of the benzyloxycarbonyl group and removal of the amine-protecting group to prepare the 1-(.omega.-amino-.alpha.-hydroxy-lower-alkanoyl) derivatives.

摘要翻译： 通过培养含有碳水化合物的营养培养基，可同化氮源，制备5-脱氧虫酰胺Cbb和6'-N-甲基-2-羟基 - 和6'-N-甲基-5-脱氧阿霉素C2b作为主要的发酵产物 ，必需盐和D-链霉胺或具有小单孢菌突变体的2,5-二隐球菌素，并从营养培养基中分离发酵产物。 在用胺保护基团首先封闭2位上的伯氨基后，将发酵产物用ω-[N-（苄氧基羰基）氨基]-α-羟基 - 低级 - 链烷酸的酯进行酰化，随后 通过催化氢解苄氧羰基并除去胺保护基以制备1-（ω-氨基-α-羟基 - 低级烷酰基）衍生物。

公开(公告)号：US4288547A

公开(公告)日：1981-09-08

申请号：US93062

申请日：1979-11-13

申请人： Haruo Yamamoto

发明人： Haruo Yamamoto

IPC分类号： A61K31/70 ,  A61K31/7028 ,  A61K31/7034 ,  A61K31/7036 ,  A61P31/04 ,  C07H15/236 ,  C12P19/48 ,  C12R1/31

CPC分类号： C12R1/31 ,  C07H15/236 ,  C12P19/485 ,  Y02P20/55 ,  Y10S435/809

摘要： 2-Hydroxygentamicins B, B.sub.1 and A.sub.3 and 2-hydroxy antibiotics JI-20A and JI-20B are prepared as fermentation products by culturing a nutrient medium containing carbohydrates, a source of assimilable nitrogen, essential salts and D-streptamine with a mutant of Micromonospora purpurea, and isolating the said fermentation products from the nutrient medium; the fermentation products are acylated with an ester of an .omega.-(N-benzyloxycarbonyl)amino-.alpha.-hydroxy-lower-alkanoic acid, after first blocking the 6'- and/or 2'-amine group with an amine-protecting group, followed by catalytic hydrogenolysis of the benzyloxycarbonyl group and removal of the amine-protecting groups to prepare the 1-N-(.omega.-amino-.alpha.-hydroxy-lower-alkanoyl) derivatives.

摘要翻译： 通过培养含有碳水化合物，可同化氮源，必需盐和D-链霉素的营养培养基，制备2-羟基阿霉素B，B1和A3和2-羟基抗生素JI-20A和JI-20B作为发酵产物，其具有小单孢菌突变体 紫外线，并从所述营养培养基中分离所述发酵产物; 在用胺保护基团首先封闭6'-和/或2'-胺基团之后，用ω-（N-苄氧羰基）氨基-α-羟基 - 低级 - 链烷酸的酯将发酵产物酰化， 然后催化氢解苄氧羰基并除去胺保护基以制备1-N-（ω-氨基-α-羟基 - 低级烷酰基）衍生物。

公开(公告)号：US4387219A

公开(公告)日：1983-06-07

申请号：US209412

申请日：1980-11-24

申请人： Haruo Yamamoto ,  Sol J. Daum

发明人： Haruo Yamamoto ,  Sol J. Daum

IPC分类号： C07H15/236 ,  C12P19/48 ,  C07H15/22

CPC分类号： C12R1/31 ,  C07H15/236 ,  C12P19/485 ,  Y02P20/55

摘要： 2-Hydroxygentamicins B, B.sub.1 and A.sub.3 and 2-hydroxy antibiotics JI-20A and JI-20B are prepared as fermentation products by culturing a nutrient medium containing carbohydrates, a source of assimilable nitrogen, essential salts and D-streptamine with a mutant of Micromonospora purpurea, and isolating the said fermentation products from the nutrient medium; the fermentation products are acylated with an ester of an .omega.-(N-benzyloxycarbonyl)amino-.alpha.-hydroxy-lower-alkanoic acid, after first blocking the 6'-and/or 2'-amine group with an amine-protecting group, followed by catalytic hydrogenolysis of the benzyloxycarbonyl group and removal of the amine-protecting groups to prepare the 1-N-(.omega.-amino-.alpha.-hydroxy-lower-alkanoyl) derivatives.

摘要翻译： 通过培养含有碳水化合物，可同化氮源，必需盐和D-链霉素的营养培养基，制备2-羟基阿霉素B，B1和A3和2-羟基抗生素JI-20A和JI-20B作为发酵产物，其具有小单孢菌突变体 紫外线，并从所述营养培养基中分离所述发酵产物; 在用胺保护基团首先封闭6'-和/或2'-胺基团之后，用ω-（N-苄氧基羰基）氨基-α-羟基 - 低级 - 链烷酸的酯将发酵产物酰化， 然后催化氢解苄氧羰基并除去胺保护基以制备1-N-（ω-氨基-α-羟基 - 低级烷酰基）衍生物。

公开(公告)号：US3909356A

公开(公告)日：1975-09-30

申请号：US50072074

申请日：1974-08-26

申请人： KYOWA HAKKO KOGYO KK

发明人： SUZUKI TAKEO ,  ITO SEIGA

IPC分类号： C07H13/06 ,  C12P7/62 ,  C12P19/00 ,  C12D13/02

CPC分类号： C12R1/06 ,  C07H13/06 ,  C12P7/62 ,  C12P19/00 ,  C12R1/15 ,  C12R1/31 ,  C12R1/34 ,  C12R1/365 ,  Y10S435/83 ,  Y10S435/843 ,  Y10S435/866 ,  Y10S435/869 ,  Y10S435/872

摘要： A process for producing fatty acid esters of fructose including the steps of (a) culturing a fructose-utilizing microorganism capable of producing fatty acid esters of fructose and belonging to the genus Arthrobacter, Corynebacterium, Nocardia or Mycobacterium in a medium containing fructose as a carbon source, (b) forming the fatty acid esters of fructose intracellularly and (c) recovering the esters from the microbial cells.

摘要翻译： 一种制备果糖的脂肪酸酯的方法，包括以下步骤：（a）在含有果糖作为碳的培养基中培养能够产生果糖并属于节杆菌属，棒状杆菌属，诺卡氏菌属或分枝杆菌属的果糖的微生物微生物 来源，（b）在细胞内形成果糖的脂肪酸酯，（c）从微生物细胞中回收酯。