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1.发明公开公开(公告)号：US20240318209A1
公开(公告)日：2024-09-26
申请号：US18627530
申请日：2024-04-05
发明人： Tianwei Tan , Yingyue Yu , Haowen Zhu , Jianyu Long
CPC分类号： C12P7/22 , C12N1/205 , C12N9/0008 , C12N9/1007 , C12N9/1022 , C12N9/1029 , C12N9/1085 , C12N9/1288 , C12N9/14 , C12R2001/15 , C12Y102/99006 , C12Y201/01014 , C12Y202/01001 , C12Y203/01031 , C12Y205/01006 , C12Y205/01054 , C12Y207/08007 , C12Y303/01001
摘要： The present invention discloses a genetically engineered bacterium using glucose as a substrate for de novo synthesis of vanillin and an application thereof, which belongs to the technical field of gene recombination and metabolic engineering. The genetically engineered bacterium using the glucose as the substrate for de novo synthesis of vanillin disclosed by the present invention is recombinant Corynebacterium glutamicum modified by chassis microorganisms and including a vanillin synthesis module and a methyl cyclic regeneration module. The genetically engineered bacteria constructed by the present invention are safe and non-toxic, can use the glucose for de novo synthesis of natural vanillin, and is low in production cost, high in yield, and promising in application prospect.
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公开(公告)号：US20230272442A1
公开(公告)日：2023-08-31
申请号：US18015840
申请日：2021-06-30
发明人： Mingan Shi , Xin Xiong , Jia Sun , Jing Zuo , Yunchong Xia , Zailing Zhu , Zhigang Cai , Guoying Zhang , Marko Blazlc , Tina Kogej , Gregor Kosec , Mirjan Svageij , Jaka Horvat , Stefan Fujs
CPC分类号： C12P17/182 , C12N9/1007 , C12N9/0028 , C12N9/93 , C12N1/20 , C12N15/52 , C12N15/75 , C12Y201/01014 , C12Y105/0102 , C12Y603/02012 , C12Y603/02017
摘要： The present invention provides a 5-methylfolate producing microorganism which a) has been modified to have a decreased expression and/or activity of a polypeptide having both dihydrofolate synthase activity and folylpolyglutamate synthetase activity compared to an otherwise identical microorganism (reference microorganism); b) has been (further) modified to express a heterologous polypeptide having only dihydrofolate synthase activity; c) has been (further) modified to have an increased expression level of at least one enzyme (such as at least two, at least three, at least four, at least five, at least six, at least seven or at least eight) enzymes involved in the biosynthesis of a 5-methylfolate compared to an otherwise identical microorganism (reference microorganism); and/or d) has been (further) modified to have a decreased expression and/or activity of a polypeptide having 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity compared to an otherwise identical microorganism (reference microorganism).
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公开(公告)号：US20140178930A1
公开(公告)日：2014-06-26
申请号：US14180975
申请日：2014-02-14
申请人： Novozymes A/S
CPC分类号： C12N15/75 , C12N9/1007 , C12N9/2471 , C12P21/00 , C12Y201/01014 , C12Y302/01023
摘要： A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
摘要翻译： 公开了一种细菌宿主细胞，其包含在其基因组中的至少两个拷贝的扩增单元，所述扩增单元包括：i）目的基因的至少一个拷贝，和ii）可表达条件必需基因，其中所述条件必需基因 是无活性的或从具有基本上低于条件性必需基因的内源启动子的活性的异源启动子转录，并且其中条件必需基因如果不起作用将使细胞营养缺陷至少一种特定物质或不能利用一种或 更具体的唯一碳源; 使用本发明的细胞产生蛋白质的方法以及构建本发明的细胞的方法。
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公开(公告)号：US20160362698A1
公开(公告)日：2016-12-15
申请号：US15252548
申请日：2016-08-31
申请人： Novozymes A/S
CPC分类号： C12N15/75 , C12N9/1007 , C12N9/2471 , C12P21/00 , C12Y201/01014 , C12Y302/01023
摘要： A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
摘要翻译： 公开了一种细菌宿主细胞，其包含在其基因组中的至少两个拷贝的扩增单元，所述扩增单元包括：i）目的基因的至少一个拷贝，和ii）可表达条件必需基因，其中所述条件必需基因 是无活性的或从具有基本上低于条件性必需基因的内源启动子的活性的异源启动子转录，并且其中条件必需基因如果不起作用将使细胞营养缺陷至少一种特定物质或不能利用一种或 更具体的唯一碳源; 使用本发明的细胞产生蛋白质的方法以及构建本发明的细胞的方法。
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公开(公告)号：US08685653B2
公开(公告)日：2014-04-01
申请号：US12712537
申请日：2010-02-25
CPC分类号： C12N15/75 , C12N9/1007 , C12N9/2471 , C12P21/00 , C12Y201/01014 , C12Y302/01023
摘要： A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
摘要翻译： 公开了一种细菌宿主细胞，其包含在其基因组中的至少两个拷贝的扩增单元，所述扩增单元包括：i）目的基因的至少一个拷贝，和ii）可表达条件必需基因，其中所述条件必需基因 是无活性的或从具有基本上低于条件性必需基因的内源启动子的活性的异源启动子转录，并且其中条件必需基因如果不起作用将使细胞营养缺陷至少一种特定物质或不能利用一种或 更具体的唯一碳源; 使用本发明的细胞产生蛋白质的方法以及构建本发明的细胞的方法。
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公开(公告)号：US09458468B2
公开(公告)日：2016-10-04
申请号：US14180975
申请日：2014-02-14
申请人： Novozymes A/S
CPC分类号： C12N15/75 , C12N9/1007 , C12N9/2471 , C12P21/00 , C12Y201/01014 , C12Y302/01023
摘要： A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
摘要翻译： 公开了一种细菌宿主细胞，其包含在其基因组中的至少两个拷贝的扩增单元，所述扩增单元包括：i）目的基因的至少一个拷贝，和ii）可表达条件必需基因，其中所述条件必需基因 是无活性的或从具有基本上低于条件性必需基因的内源启动子的活性的异源启动子转录，并且其中条件必需基因如果不起作用将使细胞营养缺陷至少一种特定物质或不能利用一种或 更具体的唯一碳源; 使用本发明的细胞产生蛋白质的方法以及构建本发明的细胞的方法。
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公开(公告)号：US07700322B2
公开(公告)日：2010-04-20
申请号：US10575697
申请日：2004-10-29
CPC分类号： C12N15/75 , C12N9/1007 , C12N9/2471 , C12P21/00 , C12Y201/01014 , C12Y302/01023
摘要： A bacterial host cell comprising at least two copies of an amplification unit in its genome, said amplification unit comprising: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of said conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
摘要翻译： 一种细菌宿主细胞，其在其基因组中包含至少两个拷贝的扩增单元，所述扩增单元包含：i）目的基因的至少一个拷贝，和ii）可表达的条件必需基因，其中条件必需基因是 来自具有显着低于所述条件必需基因的内源启动子的活性的异源启动子的无启动子或转录，并且其中所述条件必需基因如果不是功能性的将使细胞对于至少一种特定物质具有营养缺陷型或不能利用一种或多种特异性 唯一碳源 使用本发明的细胞产生蛋白质的方法以及构建本发明的细胞的方法。
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公开(公告)号：US20070134760A1
公开(公告)日：2007-06-14
申请号：US10575697
申请日：2004-10-29
申请人： Carsten Olsen , Michael Rasmussen
发明人： Carsten Olsen , Michael Rasmussen
CPC分类号： C12N15/75 , C12N9/1007 , C12N9/2471 , C12P21/00 , C12Y201/01014 , C12Y302/01023
摘要： A bacterial host cell comprising at least two copies of an amplification unit in its genome, said amplification unit comprising: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of said conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
摘要翻译： 一种细菌宿主细胞，其在其基因组中包含至少两个拷贝的扩增单元，所述扩增单元包含：i）目的基因的至少一个拷贝，和ii）可表达的条件必需基因，其中条件必需基因是 来自具有显着低于所述条件必需基因的内源启动子的活性的异源启动子的无启动子或转录，并且其中所述条件必需基因如果不是功能性的将使细胞对于至少一种特定物质具有营养缺陷型或不能利用一种或多种特异性 唯一碳源 使用本发明的细胞产生蛋白质的方法以及构建本发明的细胞的方法。
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公开(公告)号：US09506093B2
公开(公告)日：2016-11-29
申请号：US14408378
申请日：2012-06-18
申请人： Wanda Dischert , Rainer Figge
发明人： Wanda Dischert , Rainer Figge
CPC分类号： C12P13/12 , C12N9/1007 , C12Y201/01014 , Y02P20/52
摘要： The present invention is related to a recombinant microorganism optimized for the fermentative production of methionine, wherein the activity of the cobalamin-independent methionine synthase MetE is attenuated in said microorganism. The invention is also related to a method for producing methionine by fermentation.
摘要翻译： 本发明涉及针对甲硫氨酸的发酵生产而优化的重组微生物，其中所述微生物中不含钴胺素的甲硫氨酸合酶MetE的活性减弱。 本发明还涉及通过发酵生产甲硫氨酸的方法。
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10.发明申请公开(公告)号：US20150247175A1
公开(公告)日：2015-09-03
申请号：US14408378
申请日：2012-06-18
申请人： Metabolic Explorer
发明人： Wanda Dischert , Rainer Figge
CPC分类号： C12P13/12 , C12N9/1007 , C12Y201/01014 , Y02P20/52
摘要： The present invention is related to a recombinant microorganism optimised for the fermentative production of methionine, wherein the activity of the cobalamin-independent methionine synthase MetE is attenuated in said microorganism. The invention is also related to a method for producing methionine by fermentation.
摘要翻译： 本发明涉及针对甲硫氨酸的发酵生产而优化的重组微生物，其中所述微生物中不含钴胺素的甲硫氨酸合酶MetE的活性减弱。 本发明还涉及通过发酵生产甲硫氨酸的方法。
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