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公开(公告)号：US20060230467A1
公开(公告)日：2006-10-12
申请号：US10519395
申请日：2003-06-24
CPC分类号： A61K45/06 , A61K38/00 , C12N9/1205 , C12N9/1211 , C12N15/8261 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to pharmaceutical compositions comprising plant deoxyribonucleoside kinase enzymes (dNK) capable of phosphorylating nucleoside analogs and to medical use of said dNKs. More specifically the invention relates to the medical use of deoxyribonucleoside kinase enzymes derived from (Arabidopsis thaliana), from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). The invention also relates to methods of sensitising cells to prodrugs, and to methods of inhibiting pathogenic agents in warm-blooded animals using said plant dNKs. In another aspect the invention relates to plant derived deoxyribonucleoside kinase enzymes provided in isolated form from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). In further aspects the invention provides polynucleotides encoding the plant dNKs, vector constructs comprising the polynucleotide, packaging cell lines capable of producing said vector, and genetically modified isolated host cells transduced/transfected/-transformed with the vector.
摘要翻译： 本发明涉及包含能够磷酸化核苷类似物的植物脱氧核糖核苷激酶（dNK）和所述dNK的医学用途的药物组合物。 更具体地，本发明涉及来自（拟南芥），来自火炬松（Pinus taeda），番茄（Lycopersicum esculentum），玉米（Zea mays）或来自稻（Oryza sativa）的脱氧核糖核苷激酶的医学用途。 本发明还涉及使细胞对前药敏感的方法，以及使用所述植物dNK在温血动物中抑制病原体的方法。 在另一方面，本发明涉及以番茄（番茄），玉米（Zea mays）或稻（Oryza sativa）分离形式提供的植物衍生的脱氧核糖核苷激酶。 在其它方面，本发明提供了编码植物dNK的多核苷酸，包含多核苷酸的载体构建体，能够产生所述载体的包装细胞系以及用载体转导/转染/转化的遗传修饰的分离的宿主细胞。
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公开(公告)号：US07666639B2
公开(公告)日：2010-02-23
申请号：US10519395
申请日：2003-06-24
CPC分类号： A61K45/06 , A61K38/00 , C12N9/1205 , C12N9/1211 , C12N15/8261 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to pharmaceutical compositions comprising plant deoxyribonucleoside kinase enzymes (dNK) capable of phosphorylating nucleoside analogs and to medical use of said dNKs. More specifically the invention relates to the medical use of deoxyribonucleoside kinase enzymes derived from (Arabidopsis thaliana), from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). The invention also relates to methods of sensitizing cells to prodrugs, and to methods of inhibiting pathogenic agents in warm-blooded animals using said plant dNKs.In another aspect the invention relates to plant derived deoxyribonucleoside kinase enzymes provided in isolated form from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). In further aspects the invention provides polynucleotides encoding the plant dNKs, vector constructs comprising the polynucleotide, packaging cell lines capable of producing said vector, and genetically modified isolated host cells transduced/transfected/-transformed with the vector.
摘要翻译： 本发明涉及包含能够磷酸化核苷类似物的植物脱氧核糖核苷激酶（dNK）和所述dNK的医学用途的药物组合物。 更具体地，本发明涉及来自（拟南芥），来自火炬松（Pinus taeda），番茄（Lycopersicum esculentum），玉米（Zea mays）或来自稻（Oryza sativa）的脱氧核糖核苷激酶的医学用途。 本发明还涉及使细胞对前药敏感的方法，以及使用所述植物dNK在温血动物中抑制病原体的方法。 在另一方面，本发明涉及以番茄（番茄），玉米（Zea mays）或稻（Oryza sativa）分离形式提供的植物衍生的脱氧核糖核苷激酶。 在其它方面，本发明提供了编码植物dNK的多核苷酸，包含多核苷酸的载体构建体，能够产生所述载体的包装细胞系以及用载体转导/转染/转化的遗传修饰的分离的宿主细胞。
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公开(公告)号：US07928206B2
公开(公告)日：2011-04-19
申请号：US10515417
申请日：2003-05-21
CPC分类号： C12N15/8261 , A61K31/7072 , A61K38/45 , A61K45/06 , C12N9/1205 , C12N9/1211 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to novel plant thymidine kinases and their use in gene therapy. More specifically the invention provides novel thymidine kinases derived from tomato.In further aspects the invention provides novel polynucleotides encoding the tomato thymidine kinase of mutant thereof, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, methods of inhibiting pathogenic agents in warm-blooded animals, methods for biocontrol of plants, methods of synthesizing monophosphates and pharmaceutical compositions comprising the plant thymidine kinases of the invention.In a preferred embodiment the invention provides a unique combination of a plant thymidine kinase and the nucleoside analog nucleoside analog AZT (3′-azido-3′-deoxythymidine) to treat abnormal cell growth.
摘要翻译： 本发明涉及新型植物胸苷激酶及其在基因治疗中的应用。 更具体地，本发明提供了源自番茄的新型胸苷激酶。 在另外的方面，本发明提供了编码其突变体的番茄胸苷激酶的新型多核苷酸，包含多核苷酸的载体构建体，携带多核苷酸或载体的宿主细胞，使前体药物致敏细胞的方法，在温血动物中抑制病原体的方法 用于植物的生物防治，合成单磷酸盐的方法和包含本发明的植物胸苷激酶的药物组合物。 在优选的实施方案中，本发明提供植物胸苷激酶和核苷类似物核苷类似物AZT（3'-叠氮基-3'-脱氧胸苷）的独特组合来治疗异常细胞生长。
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4.发明申请公开(公告)号：US20070248543A1
公开(公告)日：2007-10-25
申请号：US11629526
申请日：2005-06-22
申请人： Zoran Gojkovic
发明人： Zoran Gojkovic
IPC分类号： A61K31/7052 , A61K39/395 , A61K48/00 , A61K49/00 , C07H21/04 , C07K14/00 , C12N1/21 , C12N15/00 , C12N5/06 , C12P1/04
CPC分类号： C12N9/1205 , A61K38/00 , A61K48/00 , C12N9/12 , C12Y207/01074 , C12Y207/01076
摘要： The application relates to the field of suicide gene therapy using expression vectors encoding a deoxynucleotide kinase capable of converting prodrugs into cytotoxic drugs. In particular, Chicken deoxycytidine kinase and eukaryotic deoxyadenosine kinase polypeptides and nucleotides encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing such polypeptides for the treatment of malignancies and viral infections, methods of sensitising cells to prodrugs, and methods of inhibiting pathogenic agents in warm-blooded animals using said dCKs and dAKs.
摘要翻译： 该申请涉及使用能够将前药转化为细胞毒性药物的脱氧核苷酸激酶的编码载体的自杀基因治疗领域。 特别地，公开了鸡脱氧胞苷激酶和真核脱氧腺苷激酶多肽和编码这些多肽的核苷酸以及通过重组技术产生这种多肽的方法。 还公开了利用这种多肽治疗恶性肿瘤和病毒感染的方法，使细胞对前药敏感的方法，以及使用所述dCK和dAK在温血动物中抑制病原体的方法。
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公开(公告)号：US20060035850A1
公开(公告)日：2006-02-16
申请号：US10515417
申请日：2003-05-21
CPC分类号： C12N15/8261 , A61K31/7072 , A61K38/45 , A61K45/06 , C12N9/1205 , C12N9/1211 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to novel plant thymidine kinases and their use in gene therapy. More specifically the invention provides novel thymidine kinases derived from tomato, pine, rice or thale cress. In further aspects the invention provides novel polynucleotides encoding the plant thymidine kinases, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, methods of inhibiting pathogenic agents in warm-blooded animals, methods for biocontrol of plants, methods of synthesizing monophosphates and pharmaceutical compositions comprising the plant thymidine kinases of the invention. In a preferred embodiment the invention provides a unique combination of a plant thymidine kinase and the nucleoside analog AZT to treat abnormal cell growth.
摘要翻译： 本发明涉及新型植物胸苷激酶及其在基因治疗中的应用。 更具体地，本发明提供了衍生自番茄，松树，水稻或鲟鱼水芹的新型胸苷激酶。 在另外的方面，本发明提供了编码植物胸苷激酶的新型多核苷酸，包含多核苷酸的载体构建体，携带多核苷酸或载体的宿主细胞，使前体药物致敏细胞的方法，温血动物中抑制病原体的方法，生物防治方法 植物，合成单磷酸盐的方法和包含本发明的植物胸苷激酶的药物组合物。 在优选的实施方案中，本发明提供植物胸苷激酶和核苷类似物AZT的独特组合以治疗异常细胞生长。
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