公开(公告)号：US06562340B1

公开(公告)日：2003-05-13

申请号：US08507362

申请日：1995-08-16

申请人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

发明人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

IPC分类号： A61K3847

CPC分类号： C12Y302/01004 ,  A23K20/189 ,  C12N9/2437 ,  C12N15/80 ,  C12Y302/01021 ,  C12Y302/01091

摘要： The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, tricale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是一种或多种EGI，EGII EGIII及其任何功能活性的衍生物。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。 这种基于酶的饲料添加剂可以掺入包括一种或多种大麦，小麦，三倍体，黑麦和玉米的谷类饲料中。 饲料添加剂具有提高饲料转化率和/或增加其中包含谷物饲料的消化率的优点。

公开(公告)号：US06447771B1

公开(公告)日：2002-09-10

申请号：US09370265

申请日：1999-08-09

申请人： John Ford ,  Julio J. Mulero ,  George Yeung

发明人： John Ford ,  Julio J. Mulero ,  George Yeung

IPC分类号： A61K3847

CPC分类号： C07K14/70596 ,  A01K2217/05 ,  A01K2217/075 ,  A61K38/00 ,  C12N9/14 ,  C12Q1/6883

摘要： The invention provides polynucleotides isolated from cDNA libraries of human fetal liver-spleen and macrophage as well as polypeptides encoded by these polynucleotides and mutants or variants thereof. The polypeptides correspond to a human CD39-like protein. Other aspects of the invention include vectors containing polynucleotides of the invention and related host cells as well a processes for producing CD39-like polypeptides, and antibodies specific for such polypeptides.

摘要翻译： 本发明提供从人胎儿肝脾和巨噬细胞的cDNA文库中分离的多核苷酸，以及由这些多核苷酸及其突变体或变体编码的多肽。 多肽对应于人CD39样蛋白。 本发明的其它方面包括含有本发明多核苷酸和相关宿主细胞的载体以及产生CD39样多肽的方法，以及对这些多肽具有特异性的抗体。

公开(公告)号：US06251390B1

公开(公告)日：2001-06-26

申请号：US07919784

申请日：1992-07-27

申请人： Gary E. Harman ,  Roxanne M. Broadway ,  Arne Tronsmo ,  Matteo Lorito ,  Christopher K. Hayes ,  Antonio Di Pietro

发明人： Gary E. Harman ,  Roxanne M. Broadway ,  Arne Tronsmo ,  Matteo Lorito ,  Christopher K. Hayes ,  Antonio Di Pietro

IPC分类号： A61K3847

CPC分类号： C12Y302/01052 ,  A01N63/00 ,  C12N9/2442 ,  C12Y302/01014

摘要： Two chitinases from Trichoderma harzianum P1 (ATCC 74058) show chitin-containing-fungus-inhibiting activity. One is an endochitinase and the other is a chitobiase. Both have molecular weights of 40 kDa and isoelectric points of 3.9. A &lgr;gtll recombinant encoding for the endochitinase has been prepared (ATCC 55338) and the gene encoding for the endochitinase has been removed from the DNA of the recombinant by the restriction enzyme, Not I. Endochitinases and chitobiases including the two purified from Trichoderma harzianum strain P1 demonstrate synergy with each other in anti-fungal effect.

摘要翻译： 来自哈茨木霉（Trichoderma harzianum P1）（ATCC 74058）的两种几丁质酶显示出含有几丁质的真菌抑制活性。 一种是内切壳酶，另一种是木质纤维素酶。 两者的分子量为40kDa，等电点为3.9。 已经制备了编码内切素酶的羔羊重组体编码（ATCC 55338），并且已经通过限制性内切酶Not I从内含子酶的DNA中除去内切素酶的基因。内含子酶和木质素酶包括从哈茨木霉菌株P1纯化的两种 在抗真菌作用中表现出彼此的协同作用。

公开(公告)号：US06344196B1

公开(公告)日：2002-02-05

申请号：US09634088

申请日：2000-08-08

申请人： Alan E. Kligerman

发明人： Alan E. Kligerman

IPC分类号： A61K3847

CPC分类号： C12Y302/01022 ,  A23L29/06 ,  A61K38/47

摘要： A composition is provided for ingestion by mammals for in vivo conversion of alpha-D-galactoside comprising an amount of alpha-galactosidase effective to hydrolyze alpha-D-galactoside to D-galactose, and non-toxic, ingestible excipient(s) for said alpha-galactosidase. Gastric distress in mammals due to ingestion of foods containing alpha-D-galactoside may be reduced by ingesting the foregoing composition of alpha-galactosidase and non-toxic, ingestible excipient contemporaneously with the ingestion of said food in an amount effective to hydrolyze the alpha-D-galactoside to D-galactose.

摘要翻译： 本发明提供一种哺乳动物摄取用于体内转化α-D-半乳糖苷的组合物，其包含一定量的有效水解α-D-半乳糖苷的D-半乳糖苷，以及无毒，可摄取的赋形剂，用于所述 α-半乳糖苷酶。 通过摄入含有α-D-半乳糖苷的食物的哺乳动物的胃窘迫可以通过摄取上述组合物的α-半乳糖苷酶和无毒的可摄取的赋形剂，同时摄入所述食物以有效水解α- D-半乳糖苷与D-半乳糖。

公开(公告)号：US06335013B1

公开(公告)日：2002-01-01

申请号：US09608285

申请日：2000-06-30

申请人： John Ford ,  Julio J. Mulero ,  George Yeung

发明人： John Ford ,  Julio J. Mulero ,  George Yeung

IPC分类号： A61K3847

CPC分类号： C07K14/70596 ,  A01K2217/05 ,  A01K2217/075 ,  A61K38/00 ,  C12N9/14 ,  C12Q1/6883 ,  C12Q2600/158

摘要： The invention provides novel polynucleotides isolated from cDNA libraries of human fetal liver-spleen and macrophage as well as polypeptides encoded by these polynucleotides and mutants or variants thereof. The polypeptides correspond to a novel human CD39-like protein. Other aspects of the invention include vectors containing polynucleotides of the invention and related host cells as well a processes for producing novel CD39-like polypeptides, and antibodies specific for such polypeptides.

摘要翻译： 本发明提供从人胎儿肝脾和巨噬细胞的cDNA文库中分离的新型多核苷酸，以及由这些多核苷酸及其突变体或变体编码的多肽。 多肽对应于新的人CD39样蛋白。 本发明的其它方面包括含有本发明多核苷酸和相关宿主细胞的载体以及产生新型CD39样多肽的方法，以及对这些多肽具有特异性的抗体。

公开(公告)号：US06210666B1

公开(公告)日：2001-04-03

申请号：US09176666

申请日：1998-10-21

申请人： Nobuhiro Miyamura

发明人： Nobuhiro Miyamura

IPC分类号： A61K3847

CPC分类号： C12N9/2465 ,  A61K38/00

摘要： Fabry disease results from an X-linked deficiency in the enzyme &agr;-galactosidase A. The present invention is directed to recombinant truncated forms of &agr;-galactosidase A, as well as therapeutic compositions comprising said truncated &agr;-galactosidase A which are useful, for example, to treat Fabry disease patients.

摘要翻译： 法布里病源自α-半乳糖苷酶A的X连锁缺陷。本发明涉及重组截短形式的α-半乳糖苷酶A，以及包含所述截短的α-半乳糖苷酶A的治疗组合物，其可用于例如 ，治疗法布里病患者。

公开(公告)号：US06461609B1

公开(公告)日：2002-10-08

申请号：US08790491

申请日：1997-01-29

申请人： David Hudgins Calhoun ,  George Coppola

发明人： David Hudgins Calhoun ,  George Coppola

IPC分类号： A61K3847

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C07K16/40 ,  C12N9/2465 ,  C12N2710/14143 ,  C12N2799/026 ,  Y02A50/473

摘要： Fabry disease results from an X-linked deficiency in the enzyme &agr;-galactosidase A. The present invention is directed to recombinant human &agr;-galactosidase A and provides baculovirus expression vectors and recombinant virus that provide stable expression of extracellular and intracellular levels of this enzyme in an insect cell culture. The recombinant-derived enzyme can be used in enzyme replacement therapy to treat Fabry patients. Composition useful in therapeutic administration of &agr;-galactosidase A are also provided.

摘要翻译： 法布里病源自α-半乳糖苷酶A的X连锁缺陷。本发明涉及重组人α-半乳糖苷酶A，并提供杆状病毒表达载体和重组病毒，其提供该酶的细胞外和细胞内水平的稳定表达 昆虫细胞培养。 重组衍生酶可用于酶替代疗法治疗法布里患者。 还提供了可用于治疗性施用α-半乳糖苷酶A的组合物。

公开(公告)号：US06426070B1

公开(公告)日：2002-07-30

申请号：US09446959

申请日：2000-05-09

申请人： Helene F. Rosenberg ,  Joseph B. Domachowske

发明人： Helene F. Rosenberg ,  Joseph B. Domachowske

IPC分类号： A61K3847

CPC分类号： A61K38/465 ,  C12Y301/27005

摘要： A method for inactivating a virion of an enveloped RNA virus comprising contacting the virion with an eosinophil-derived ribonuclease, such as eosinophil-derived neurotoxin (EDN) or eosinophil cationic protein (ECP). The invention also provides methods for treating a subject infected by an enveloped RNA virus and for preventing infection by an enveloped RNA virus comprising administering an effective amount of an eosinophil-derived ribonuclease, such as EDN or ECP. The invention also provides a composition comprising an effective amount of an eosinophil-derived ribonuclease and an acceptable carrier.

摘要翻译： 一种用于灭活包膜RNA病毒的病毒粒子的方法，包括使病毒粒子与嗜酸性粒细胞衍生的核糖核酸酶如嗜酸性粒细胞衍生的神经毒素（EDN）或嗜酸性粒细胞阳离子蛋白质（ECP）接触。 本发明还提供了治疗受包膜RNA病毒感染的受试者和用于预防包膜RNA病毒感染的方法，包括施用有效量的嗜酸性粒细胞衍生的核糖核酸酶，例如EDN或ECP。 本发明还提供了包含有效量的嗜酸性粒细胞衍生的核糖核酸酶和可接受的载体的组合物。

公开(公告)号：US06183741B2

公开(公告)日：2001-02-06

申请号：US09183149

申请日：1998-10-30

申请人： Anne G. Rowlands ,  Robert F. Rainville

发明人： Anne G. Rowlands ,  Robert F. Rainville

IPC分类号： A61K3847

CPC分类号： G03C1/047

摘要： The present invention is a process for reducing gelatin insolubles which includes providing a gelatin solution and adding to the gelatin solution amylase in an amount to provide a concentration of amylase of at least 0.1 ppm for a time sufficient to reduce gelatin insolubles. The present invention also provides a gelatin having a Bloom strength of from 60 to 400 and a concentration of amylase of greater than 0.1 ppm.

摘要翻译： 本发明是一种减少明胶不溶物的方法，其包括提供明胶溶液，并将淀粉酶的添加量提供至少0.1ppm的淀粉酶浓度足以减少明胶不溶物的时间。 本发明还提供了一种布朗强度为60-400，淀粉酶浓度大于0.1ppm的明胶。

公开(公告)号：US06743425B2

公开(公告)日：2004-06-01

申请号：US10218109

申请日：2002-08-14

申请人： Kazuwa Nakao

发明人： Kazuwa Nakao

IPC分类号： A61K3847

CPC分类号： A61K38/2242

摘要： The present invention aims to provide novel therapeutic agents for achondroplasia caused by mutations in FGFR3. Therapeutic agents for achondroplasia caused by the cartilage growth inhibition resulting from mutations in the gene for fibroblast growth factor receptor 3 (FGFR3), comprising a substance activating guanylyl cyclase B (GC-B) as an active ingredient are disclosed.

摘要翻译： 本发明旨在提供由FGFR3突变引起的软骨发育不良的新型治疗剂。用于成纤维细胞生长因子受体3（FGFR3）基因突变引起的软骨生长抑制引起的软骨发育不良的治疗剂包括活化鸟苷酸环化酶B （GC-B）作为活性成分。