公开(公告)号：US20020083496A1

公开(公告)日：2002-06-27

申请号：US09844006

申请日：2001-04-30

发明人： Sarah Wyatt ,  Pei-Lan Tsou ,  Dominique Robertson ,  Wendy Boss

IPC分类号： A01H005/00 ,  C12N015/82 ,  C12N005/14

CPC分类号： C12N15/8242 ,  C12N15/8249 ,  C12N15/8261 ,  C12N15/8266 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8274 ,  Y02A40/146

摘要： The present invention provides transgenic plants over-expressing a transgene encoding a calcium-binding protein or peptide (CaBP). Preferably, the CaBP is a calcium storage protein and over-expression thereof does not have undue adverse effects on calcium homeostasis or biochemical pathways that are regulated by calcium. In preferred embodiments, the CaBP is calreticulin (CRT) or calsequestrin. In more preferred embodiments, the CaBP is the C-domain of CRT, a fragment of the C-domain, or multimers of the foregoing. In other preferred embodiments, the CaBP is localized to the endoplasmic reticulum by operatively associating the transgene encoding the CaBP with an endoplasmic reticulum localization peptide. Alternatively, the CaBP is targeted to any other sub-cellular compartment that permits the calcium to be stored in a form that is biologically available to the plant. Also provided are methods of producing plants with desirable phenotypic traits by transformation of the plant with a transgene encoding a CaBP. Such phenotypic traits include increased calcium storage, enhanced resistance to calcium-limiting conditions, enhanced growth and viability, increased disease and stress resistance, enhanced flower and fruit production, reduced senescence, and a decreased need for fertilizer production. Further provided are plants with enhanced nutritional value as human food or animal feed.

摘要翻译： 本发明提供过表达编码钙结合蛋白或肽（CaBP）的转基因的转基因植物。 优选地，CaBP是钙储存蛋白，并且其过度表达对钙调节的钙稳态或生化途径没有不利的不利影响。 在优选的实施方案中，CaBP是钙网蛋白（CRT）或钙蛋白酶。 在更优选的实施方案中，CaBP是CRT的C-结构域，C-结构域的片段或前述的多聚体。 在其它优选实施方案中，CaBP通过将编码CaBP的转基因与内质网定位肽有效结合而定位于内质网。 或者，CaBP靶向任何其他亚细胞区室，其允许钙以对植物生物学可用的形式存储。 还提供了通过用编码CaBP的转基因转化植物来产生具有期望表型性状的植物的方法。 这种表型性状包括增加钙储存，增强对钙限制条件的抵抗力，增强生长和活力，增加疾病和胁迫抗性，增强花和果实生产，减少衰老，减少对肥料生产的需求。 还提供了作为人类食物或动物饲料具有增强的营养价值的植物。

公开(公告)号：US20010047525A1

公开(公告)日：2001-11-29

申请号：US09766113

申请日：2001-01-19

发明人： Wesley B. Bruce ,  Xiping Niu

IPC分类号： A01H005/00 ,  C07H021/04 ,  C12N005/14 ,  C12N015/82

CPC分类号： C12N15/8222 ,  C12N15/1034 ,  C12N15/8227

摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions are novel nucleotide sequences for root-preferred promoter elements and plant promoters comprising the elements. Methods for expressing a nucleotide sequence in a plant using the promoter sequences disclosed herein are provided. The methods comprise transforming a plant cell with a nucleotide sequence operably linked to the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物是用于根优选启动子元件的新核苷酸序列和包含这些元件的植物启动子。 提供了使用本文公开的启动子序列在植物中表达核苷酸序列的方法。 所述方法包括用与本发明的启动子可操作地连接的核苷酸序列转化植物细胞，并从转化的植物细胞再生稳定转化的植物。

公开(公告)号：US20010026940A1

公开(公告)日：2001-10-04

申请号：US09826660

申请日：2001-04-05

发明人： Guy A. Cardineau ,  Steven J. Stelman ,  Kenneth E. Narva

IPC分类号： C12N015/82 ,  C12N005/14

CPC分类号： C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  Y02A40/162

摘要： The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention provides plant-optimized, polynucleotide sequences that encode pesticidal toxins (full-length and truncated). Truncated polynucleotide sequences can be used to produce truncated toxins or for the production of fusion (or chimeric) genes and proteins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, that make them particularly well-suited for optimized expression in plants. Using techniques known to those skilled in the art, the polynucleotide sequences described herein can be used to transform plants in order to confer pest resistance upon said plants. The subject invention further provides plant-optimized, polynucleotide sequences that encode C-terminal, protoxin portions that can be used with genes encoding truncated, core toxins to produce full-length toxins. In addition, the subject invention provides preferred amino acid sequences that are encoded by the plant-optimized polynucleotide sequences disclosed herein.

摘要翻译： 本发明涉及可用于防治害虫，特别是植物害虫的材料和方法。 更具体地，本发明提供编码杀虫毒素（全长和截短的）的植物优化的多核苷酸序列。 截短的多核苷酸序列可用于产生截短的毒素或用于产生融合（或嵌合）基因和蛋白质。 与野生型序列相比，本发明的多核苷酸序列具有某些修饰，使得它们特别适合于在植物中优化表达。 使用本领域技术人员已知的技术，本文所述的多核苷酸序列可用于转化植物以赋予所述植物上的害虫抗性。 本发明进一步提供植物优化的编码C末端原毒素部分的多核苷酸序列，其可以与编码截短的核心毒素的基因一起使用以产生全长毒素。 此外，本发明提供了由本文公开的植物优化的多核苷酸序列编码的优选的氨基酸序列。

公开(公告)号：US20040221333A1

公开(公告)日：2004-11-04

申请号：US10682915

申请日：2003-10-14

发明人： Annemie Boets ,  Greta Arnaut ,  Jeroen Van Rie ,  Nicole Damme

IPC分类号： C12N015/82 ,  A01H001/00 ,  C12N015/87 ,  A01H005/00 ,  C12N005/14 ,  C12N015/31

CPC分类号： C07K14/32 ,  C12N15/8286 ,  Y02A40/162

摘要： Novel bacterial insecticidal proteins and equivalents thereof were isolated. These proteins and the DNA sequences encoding them are useful to make insecticidal compositions or transgenic plants to protect plants from damage by insects, particularly coleopteran insects.

摘要翻译： 分离出新的细菌杀虫蛋白及其等同物。 这些蛋白质和编码它们的DNA序列可用于制备杀虫组合物或转基因植物，以保护植物免受昆虫特别是鞘翅目昆虫的损害。

公开(公告)号：US20020013958A1

公开(公告)日：2002-01-31

申请号：US09923876

申请日：2001-08-06

申请人： Incyte Pharmaceuticals, Inc.

发明人： Raghunath V. Lalgudi ,  Laura Y. Ito ,  Bradley K. Sherman

IPC分类号： A01H005/00 ,  C12N015/82 ,  C12N005/14 ,  C12N015/29

CPC分类号： C07K14/415

摘要： The present invention provides purified, corn seedling-derived polynucleotides (cdps) which encode corn seedling-derived polypeptides (CDPs). The invention also provides for the use of cdps or their complements, oligonucleotides, or fragments in methods for determining altered gene expression, to recover regulatory elements, and to follow inheritance of desirable characteristics through hybrid breeding programs. The invention further provides for vectors and host cells containing cdps for the expression of CDPs. The invention additionally provides for (i) use of isolated and purified CDPs to induce antibodies and to screen libraries of compounds and (ii) use of anti-CDP antibodies in diagnostic assays.

摘要翻译： 本发明提供了编码玉米幼苗衍生多肽（CDPs）的纯化的玉米秧苗衍生的多核苷酸（cdps）。 本发明还提供了在用于确定改变的基因表达，恢复调节元件以及通过杂交育种程序追踪所需特征的遗传的方法中使用cdps或其补体，寡核苷酸或片段。 本发明进一步提供含有CDP表达的CDP的载体和宿主细胞。 本发明另外提供（i）使用分离和纯化的CDP诱导抗体和筛选化合物文库和（ii）在诊断测定中使用抗CDP抗体。

公开(公告)号：US20010049833A1

公开(公告)日：2001-12-06

申请号：US09782982

申请日：2001-02-14

发明人： Andrew James Greenland ,  Didier Rene Philippe Thomas ,  Ian Jepson

IPC分类号： A01H005/00 ,  C12N015/82 ,  C12N005/14 ,  C12N005/16

CPC分类号： C12N15/8249 ,  C12N9/50 ,  C12N15/8261 ,  Y02A40/146

摘要： Disclosed are isolated DNA constructs including (a) a first DNA sequence including either an inducible promoter sequence responsive to the presence or absence of an exogenous inducer or a developmental gene promoter capable of initiating gene expression in a selected tissue or at a selected stage of development of an organism; (b) a second DNA sequence including a DNA sequence coding for a protease enzyme operably linked and under the control of the promoter sequence specified at (a); whereby the presence or absence of the exogenous inducer or the activation of the developmental gene promoter specified at (a) results in expression of the protease enzyme. These constructs are preferably rendered reversible by the presence of further elements. These constructs can be used in plant or mammalian cells for disruption of cell function, controlling senescence and modifying the metabolism of stored proteins.

摘要翻译： 公开了分离的DNA构建体，其包括（a）第一DNA序列，其包括对存在或不存在外源性诱导物的诱导型启动子序列或能够在选择的组织中或在选定的发育阶段启动基因表达的发育基因启动子 的生物体 （b）第二DNA序列，其包含编码可操作地连接并在（a）中指定的启动子序列控制下的蛋白酶的DNA序列; 由此外部诱导物的存在或不存在或（a）中规定的发育基因启动子的活化导致蛋白酶的表达。 这些构建体优选通过其它元素的存在而是可逆的。 这些构建体可用于植物或哺乳动物细胞破坏细胞功能，控制衰老和改变储存蛋白质的代谢。

公开(公告)号：US20030237110A9

公开(公告)日：2003-12-25

申请号：US09923876

申请日：2001-08-06

申请人： Incyte Pharmaceuticals, Inc.

发明人： Raghunath V. Lalgudi ,  Laura Y. Ito ,  Bradley K. Sherman

IPC分类号： A01H005/00 ,  C12N015/82 ,  C12N005/14 ,  C12N015/29

CPC分类号： C07K14/415

摘要： The present invention provides purified, corn seedling-derived polynucleotides (cdps) which encode corn seedling-derived polypeptides (CDPs). The invention also provides for the use of cdps or their complements, oligonucleotides, or fragments in methods for determining altered gene expression, to recover regulatory elements, and to follow inheritance of desirable characteristics through hybrid breeding programs. The invention further provides for vectors and host cells containing cdps for the expression of CDPs. The invention additionally provides for (i) use of isolated and purified CDPs to induce antibodies and to screen libraries of compounds and (ii) use of anti-CDP antibodies in diagnostic assays.

公开(公告)号：US20020002712A1

公开(公告)日：2002-01-03

申请号：US09847089

申请日：2001-05-01

发明人： Mary C. Betlach ,  James T. Kealey ,  Neal Gutterson ,  Ed Ralston

IPC分类号： C12N015/82 ,  C12N005/14 ,  A01H005/00

CPC分类号： C12N9/1258 ,  C12N15/52 ,  C12N15/8243

摘要： The present invention provides genetically altered plants and plant cells that have been modified to contain expression system(s) capable of expressing a functional polyketide synthase (PKS). The present invention further provides methods of producing PKS and polyketides using these plants and cells.

摘要翻译： 本发明提供已被修饰以包含能够表达功能性聚酮化合物合酶（PKS）的表达系统的经遗传改变的植物和植物细胞。 本发明还提供使用这些植物和细胞产生PKS和聚酮化合物的方法。

公开(公告)号：US20010039670A1

公开(公告)日：2001-11-08

申请号：US09834624

申请日：2001-04-16

申请人： BTG INTERNATIONAL LIMITED.

发明人： Maria Deak ,  Denes Dudits ,  Karolyne Torok ,  Laszlo Sass ,  Barna Balazs ,  Zoltan Kiraly

IPC分类号： C12N015/82 ,  C12N005/14 ,  C12N015/29 ,  A01H005/00

CPC分类号： C12N15/8271 ,  C07K14/415 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283

摘要： The invention relates to plants, especially transgenic plants, plant parts and plant cells overproducing an iron binding protein (e.g. ferritin) and having an enhanced resistance against a wide range of abiotic and biotic oxidative stress conditions (e.g. against treatment with paraquat or fusaric acid and against viral, bacterial and fungal infections). The invention also comprises nucleic acid sequences encoding an alfalfa ferritin or functional variants thereof and the use of said sequences for rendering plants resistant against oxidative stress conditions. The invention is useful for reducing environmental damages of crops caused by a wide variety of stress conditions.

摘要翻译： 本发明涉及植物，特别是转基因植物，过度产生铁结合蛋白（例如铁蛋白）的植物部分和植物细胞，并且具有对广泛范围的非生物和生物氧化应激条件的增强的抗性（例如，抗百草枯或禾本科酸和 针对病毒，细菌和真菌感染）。 本发明还包括编码苜蓿铁蛋白或其功能变体的核酸序列，以及所述序列用于提供抗氧化应激条件的植物的用途。 本发明对于减少由各种胁迫条件引起的作物的环境损害是有用的。

公开(公告)号：US20040221331A1

公开(公告)日：2004-11-04

申请号：US10713381

申请日：2003-11-14

发明人： Marc C. Albertsen ,  Timothy W. Fox ,  Carl W. Garnaat ,  Gary Huffman ,  Timmy L. Kendall

IPC分类号： C07H021/04 ,  A01H001/00 ,  C12N015/82 ,  C12N015/87 ,  C12N015/00 ,  A01H005/00 ,  C12N015/63 ,  C12N005/14

CPC分类号： C12Y302/01031 ,  C07K14/34 ,  C07K14/415 ,  C12N9/1037 ,  C12N9/22 ,  C12N9/2434 ,  C12N15/8231 ,  C12N15/8289 ,  C12Y602/01003

摘要： The present invention relates to an isolated nucleic acid sequence encoding the Ms45 male tissue-preferred regulatory region. In one aspect this invention relates the use of this male tissue-preferred regulatory region in mediating fertility. An example of such use is the production of hybrid seed such as in a male sterility system. The Ms45 male tissue-preferred regulatory region can be operably linked with exogenous genes, such as those encoding cytotoxins, complementary nucleotidic units and inhibitory molecules. This invention also relates to plant cells, plant tissue and differentiated plants which contain the regulatory region in this invention.