公开(公告)号：US07858560B2

公开(公告)日：2010-12-28

申请号：US10197954

申请日：2002-07-16

申请人： Hubert Köster ,  Suhaib Siddiqi ,  Daniel P. Little

发明人： Hubert Köster ,  Suhaib Siddiqi ,  Daniel P. Little

IPC分类号： C40B44/00

CPC分类号： G01N33/6803 ,  B01J2219/00576 ,  B01J2219/00581 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6834 ,  C40B40/06 ,  C40B40/10 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6851 ,  C12Q2565/627 ,  C12Q2565/518

摘要： Capture Collections of capture compounds and collections thereof and methods using the compounds collections for the analysis of biomolecules are provided. In particular, collections, compounds and methods are provided for analyzing complex protein mixtures, such as the proteome. The compounds in the collections are multifunctional reagents that provide for the separation and isolation of biomolecules in complex protein mixtures. In the collections, each and every compound in the collection, has the formula: Z is a trityl derivative, each of m and n independently is an integer that is 1 to 100; X, the reactivity function, covalently binds to amino acid side chains of biomolecules; Y, the selectivity function, modulates binding of X to the amino acid side chains in biomolecules such that X binds to fewer biomolecules when the selectivity moiety Y is present than in its absence; and Q permits separation or immobilization of capture compounds in the collection. Automated systems for performing the methods also are provided.

摘要翻译： 捕获捕获化合物及其收集物的集合以及使用化合物集合分析生物分子的方法。 特别地，提供收集，化合物和方法用于分析复合蛋白质混合物，例如蛋白质组。 收集物中的化合物是多功能试剂，其提供复杂蛋白质混合物中生物分子的分离和分离。 在集合中，集合中的每个化合物具有以下分子式：Z是三苯甲基衍生物，m和n各自独立地是1至100的整数; X，反应性功能共价结合生物分子的氨基酸侧链; Y，选择性功能，调节X与生物分子中氨基酸侧链的结合，使得当存在选择性部分Y时，X与不存在的选择性部分Y结合较少的生物分子; 并且Q允许在收集物中分离或固定捕获化合物。 还提供了用于执行方法的自动化系统。