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    Solid phase sequencing of biopolymers
    3.
    发明授权
    Solid phase sequencing of biopolymers 有权
    生物聚合物的固相测序

    公开(公告)号：US07803529B1

    公开(公告)日：2010-09-28

    申请号：US09395409

    申请日：1999-09-14

    申请人： Charles Cantor ,  Hubert Köster

    发明人： Charles Cantor ,  Hubert Köster

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6872 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2531/113 ,  C12Q2521/525 ,  C12Q2565/627 ,  C12Q2531/119 ,  C12Q2523/301 ,  C12Q2531/137 ,  C12Q2521/301 ,  C12Q2531/149

    摘要： This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

    摘要翻译： 本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。 有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。 这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。 该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。 可以确定其序列的核酸包括生物样品中的DNA或RNA，例如患者活组织检查和环境样品。 探针可以固定在固体支持物例如杂交芯片上以促进自动化分子量分析和靶序列的鉴定。

    Mass spectrometric detection of polypeptides
    8.
    发明授权
    Mass spectrometric detection of polypeptides 有权
    多肽的质谱检测

    公开(公告)号：US06387628B1

    公开(公告)日：2002-05-14

    申请号：US09664977

    申请日：2000-09-18

    申请人： Daniel Little ,  Hubert Köster ,  G. Scott Higgins ,  David Lough

    发明人： Daniel Little ,  Hubert Köster ,  G. Scott Higgins ,  David Lough

    IPC分类号： C12Q160

    CPC分类号： G01N33/6848 ,  G01N33/6818 ,  G01N33/6842 ,  G01N33/6851 ,  H01J49/00

    摘要： A process for determining the identity of a target polypeptide using mass spectroscopy is provided. Depending on the target polypeptide to be identified, a process as disclosed can be used, for example, to diagnose a genetic disease or chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogenic organism; or for determining identity or heredity. Kits for performing the disclosed processes also are provided.

    摘要翻译： 提供了使用质谱法确定靶多肽的身份的方法。 根据待鉴定的靶多肽，可以使用所公开的方法，例如诊断遗传疾病或染色体异常，疾病或病症的倾向或病原生物的感染; 或用于确定身份或遗传。 还提供了用于执行所公开的处理的套件。