公开(公告)号：WO1998020154A1

公开(公告)日：1998-05-14

申请号：PCT/US1997019729

申请日：1997-10-29

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  KUYLEN, Nazle ,  CREWS, Harold, R. ,  JAFFE, Gerald, E. ,  BARCELON, Maria ,  LUCAS, Francis, J. ,  GARCIA, Nancy, M.

IPC: C12Q01/00

CPC classification number: C12Q1/34 ,  C12Q1/26 ,  C12Q1/37 ,  G01N33/574 ,  G01N33/582 ,  G01N2333/95 ,  G01N2333/96413 ,  G01N2333/96466 ,  G01N2333/96469 ,  G01N2800/52

Abstract: The necessity to detect blast cells in a patient sample to determine a treatment regime for leukemia has long been desired. The present invention relates to a method and assay reagents for identification of blast cells in a fluid sample. The method further enables the determination of the lineage of the blast cells. The new method provides a determination of blast cells in less time than prior art methods.

Abstract translation: 早期需要检测患者样品中的胚细胞以确定白血病的治疗方案。 本发明涉及用于鉴定流体样品中的胚细胞的方法和测定试剂。 该方法还能够确定胚细胞的谱系。 该新方法提供比现有技术方法更少的时间测定胚细胞。

公开(公告)号：WO1998018320A1

公开(公告)日：1998-05-07

申请号：PCT/US1997017847

申请日：1997-10-01

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  SHUKLA, Ravindra, S. ,  CREWS, Harold, R. ,  GALIOUNGHI, Adry ,  LANDRUM, Eileen ,  LUCAS, Francis, J.

IPC: A01N01/02

CPC classification number: G01N33/5005 ,  Y10T436/10

Abstract: The invention provides a novel cell control product for use with a cytoenzymology assay to confirm enzymatic activity in devices employing electronic and/or optical means. The cell control product comprises a lyophilized mammalian cell which is capable of being rehydrated in water to exhibit cellular structure and cellular enzymatic activity so that said lyophilized cells can function effectively as a cytoenzymology cell control in an enzymatic analysis. The cellular structure of the cell control is capable of being authenticated by light scatter or microscopy analysis and the cellular enzymatic activity is capable of being authenticated by fluorescence analysis. The cell control exhibits a real time stability when stored at between 2 and 8 DEG C for at least a two-month period of time. Preferably, the mammalian cell is an abnormal cell selected from Molt 4, CEM and HL 60 cell lines.

Abstract translation: 本发明提供了一种新的细胞控制产物，用于细胞酶学测定以确认采用电子和/或光学方法的装置中的酶活性。 细胞对照产物包括冻干的哺乳动物细胞，其能够在水中再水化以显示细胞结构和细胞酶活性，使得所述冻干细胞可以有效地起到酶分析中的细胞酶学细胞控制的作用。 细胞控制的细胞结构能够通过光散射或显微镜分析进行鉴定，并且细胞酶活性能够通过荧光分析进行鉴定。 当在2℃和8℃之间储存至少两个月的时间时，细胞控制体现出实时的稳定性。 优选地，哺乳动物细胞是选自Molt4，CEM和HL60细胞系的异常细胞。

公开(公告)号：WO1997018474A1

公开(公告)日：1997-05-22

申请号：PCT/US1996017577

申请日：1996-11-05

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  COULTER, Wallace, H. ,  ZWERNER, Robert, K. ,  SCHMITTLING, Robert, J. ,  RUSSELL, Thomas, R.

IPC: G01N33/569

CPC classification number: G01N33/5375 ,  G01N33/569 ,  G01N33/56966

Abstract: A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles (14) have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample (12) by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernantant can be further enriched by multiple removal steps.

Abstract translation: 用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。 颗粒（14）具有一个或多个与其结合的反应物，其特定于并将与所选群体结合。 颗粒优选与样品（12）混合，通过反复使颗粒通过大部分样品沉降以结合所选择的群体。 然后允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含富集群体的上清液从结合有所选群体的颗粒中分离。 通过多次去除步骤可以进一步丰富生物样品上清液中富集的种群。

公开(公告)号：WO1996034291A1

公开(公告)日：1996-10-31

申请号：PCT/US1996005941

申请日：1996-04-29

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  YOUNG, Carole

IPC: G01N33/96

CPC classification number: G01N33/96 ,  G01N15/1012 ,  G01N15/12 ,  G01N33/5094 ,  G01N33/92 ,  G01N2496/05 ,  G01N2496/10

Abstract: A method of quality control to diagnose the cause of a malfunction of an instrument. The method uses measurements of the physical property of a sample to diagnose the cause of a malfunction of an instrument. The spatial position of a control product sample is analysed. Alternatively, the spatial position of a statistically significant number of patient blood samples can be used. The method enables the monitoring of an instrument for problems associated with debris and noise caused by red cell lysis inefficiency; instrument reagents pump volume settings; instrument laser alignments; instrument gain settings; and flow noise caused by partial plugs, residual plugs or other flow problems. The method provides a more specific indication of the type and cause of an instrument malfunctioning than non specific flagging is provided by prior art methods.

Abstract translation: 一种用于诊断仪器故障原因的质量控制方法。 该方法使用样品的物理性质的测量来诊断仪器故障的原因。 分析对照产品样品的空间位置。 或者，可以使用统计学上显着数量的患者血液样本的空间位置。 该方法能够监测仪器与由红细胞裂解无效率引起的碎片和噪声相关的问题; 仪器试剂泵体积设置; 仪器激光对准 仪器增益设置; 和由部分插头，残留插头或其他流量问题引起的流动噪声。 该方法提供了比现有技术方法提供的非特定标记的仪器故障的类型和原因的更具体的指示。

公开(公告)号：WO2004019047A1

公开(公告)日：2004-03-04

申请号：PCT/US2003/024428

申请日：2003-08-06

Applicant: COULTER INTERNATIONAL CORP.

Inventor： KRAMER, Donald, L.

IPC: G01N021/00

CPC classification number: G01N15/1459 ,  G01N21/474 ,  G01N2015/1006

Abstract: A back-scatter detector for detecting light scattered backwardly (i.e., reflected) by an irradiated particle such as a blood cell comprises a plurality of optical fibers. A fiber optic holder (30) having a centrally located opening for passing a light beam used to irradiate particles at a particle-interrogation zone serves to position the light-collecting ends at a desired position to collect back-scattered light. Preferably, the light-­collecting ends of the optical fibers are positioned in a circular pattern centered about the irradiating light beam, and the respective axes of the supported portions of the optical fibers extend either parallel to the beam axis, or, more preferably, so that they converge at or near the location of the scattering source, i.e., the irradiated particle or cell. The latter configuration assures that the back-scatter light enters the fiber end substantially parallel to the fiber axis, thereby reducing optical transmission loses in the fiber.

Abstract translation: 用于检测被诸如血细胞的照射颗粒向后（即反射）的光的后向散射检测器包括多根光纤。 具有用于通过用于在粒子询问区照射颗粒的光束的居中定位的开口的光纤保持器（30）用于将光收集端部定位在期望位置以收集反向散射光。 优选地，光纤的聚光端以围绕照射光束为中心的圆形图案定位，并且光纤的被支撑部分的各个轴线平行于光束轴线延伸，或者更优选地如此 它们聚集在散射源的位置处或附近，即辐射的颗粒或细胞。 后一种配置确保背散射光基本上平行于光纤轴进入光纤端，从而减少光纤中的光传输损耗。

公开(公告)号：WO2003012427A1

公开(公告)日：2003-02-13

申请号：PCT/US2002/022415

申请日：2002-07-15

Applicant: COULTER INTERNATIONAL CORP.

Inventor： ELLIOTT, Michael ,  JACOBS, Dana, B. ,  ORTIZ, Nery ,  GOLDSON, Wayne ,  WHITLEY, Penny ,  GERULA, Theodore, J.

IPC: G01N31/00

CPC classification number: G01N33/96 ,  G01N2496/05 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/108331

Abstract: An improved blood suspension media for hematological compositions, having particular utility with a red blood cell component for devices using electronic and optical means for blood determinations, and processes for using the suspension media. The suspension media finds particular utility in providing the hematology control product with a stable and consistent MCV and RDW for an extended product shelf life.

Abstract translation: 用于血液学组合物的改进的血液悬浮培养基，其具有用于使用电子和光学手段用于血液测定的装置的红细胞组分的特别用途，以及使用悬浮介质的方法。 悬浮介质特别适用于为血液控制产品提供稳定和一致的MCV和RDW以延长产品保质期。

公开(公告)号：WO1997026528A1

公开(公告)日：1997-07-24

申请号：PCT/US1997000099

申请日：1997-01-06

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  FRANK, Robert, Stewart ,  WYATT, James, Lynn

IPC: G01N15/14

CPC classification number: G01N15/12 ,  G01N15/1031 ,  G01N2015/105 ,  G01N2015/1093 ,  G01N2015/129

Abstract: This invention is related to an apparatus and method to determine the hemoglobin concentration of individual red blood cells by measuring the radio frequency resistance (RF) and direct current (DC) resistance resulting during the flow of the cells through a measurement aperture. The hemoglobin concentration of the cell is determined using the ratio of these two resistances. This determination can be made using a focused flow cell and a non-focused flow cell. In addition, the determination can be made with red blood cells which are either in their native, deformable state or with red blood cells that have been isovolumetrically sphered.

Abstract translation: 本发明涉及通过测量在细胞通过测量孔径流动期间产生的射频电阻（RF）和直流（DC）电阻来确定各个红细胞的血红蛋白浓度的装置和方法。 使用这两个电阻的比率确定电池的血红蛋白浓度。 该确定可以使用聚焦流动池和非聚焦流动池进行。 此外，可以使用处于其天然的，可变形的状态的红血细胞或已经被等离子体喷射的红细胞进行测定。

公开(公告)号：WO1996021399A1

公开(公告)日：1996-07-18

申请号：PCT/US1996000066

申请日：1996-01-11

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  COULTER, Wallace, H. ,  SHAMBAUGH, Charles, R. ,  CARTER, James, H. ,  SHUKLA, Ravindra

IPC: A61B19/00

CPC classification number: G01N35/1079 ,  A61B5/15003 ,  A61B5/150351 ,  A61B5/150755 ,  A61J1/201 ,  A61J1/2017 ,  A61J1/2058 ,  A61J1/2072 ,  A61J1/2075 ,  A61J1/2082 ,  A61J1/2086 ,  A61J1/2089 ,  A61J1/2096 ,  B01L3/5082

Abstract: A device and method for transferring fluids between closed vials is provided. In one embodiment, a reagent transfer vial (42) is provided. In a further embodiment, the reagent transfer vial is used in conjunction with a transfer device (10) which includes a cylinder having a pair of opposing open tubes or tubular portions into which a sample vial (12) and the reagent transfer vial (42) can be inserted to transfer fluid therebetween. The tubes are separated by a wall through which extends a fluid transfer needle (40) and a vent needle (24) which first is inserted into a sample vial to vent the sample vial into a vent chamber (36) or reservoir formed in the transfer device before the transfer needle is inserted into the sample vial.

Abstract translation: 提供了用于在密封小瓶之间传送流体的装置和方法。 在一个实施方案中，提供了试剂转移小瓶（42）。 在另一个实施方案中，试剂转移小瓶与转运装置（10）结合使用，转运装置（10）包括具有一对相对的开放管或管状部分的圆筒，样品瓶（12）和试剂转移小瓶（42） 可以插入其间传送流体。 管被壁分开，通过该壁延伸流体输送针（40）和通气针（24），通气针（24）首先插入样品瓶中，以将样品瓶排出到形成在转印器中的通风室（36）或储存器 将转移针插入样品瓶中之前的装置。

公开(公告)号：WO2003096146A2

公开(公告)日：2003-11-20

申请号：PCT/US2003/012919

申请日：2003-04-25

Applicant: COULTER INTERNATIONAL CORP.

Inventor： LARY, Todd, P. ,  RILEY, John, S. ,  SNOW, Christopher, W.

IPC: G06F

CPC classification number: G06T11/206 ,  G06F19/00 ,  G06Q50/24 ,  Y10S707/99945

Abstract: A stellar icon (20) for representing multivariate data includes finger members (25) corresponding to constituents of a sample with a length corresponding to an expression of an attribute of the constituent. The finger members are positioned on the base member (23) so that the finger members corresponding to a constituent with an expression of attribute within a certain range are on the upper portion of the base member, and the finger members corresponding to a constituent with an expression of an attribute that is not in the range are positioned on the lower portion of the base member. A method and system for forming the stellar icon include forming a transformation table (47) including data regarding the range and constituents and attributes to be represented. The transformation table is used to determine the relative position of each finger member on the base member and to determine the length of the finger member so that the length corresponds to the expression of the attribute.

Abstract translation: 用于表示多变量数据的恒星图标（20）包括对应于具有与组成部分的属性的表达式相对应的长度的样本的组成部分的手指部件（25）。 指状构件位于基部构件（23）上，使得与具有一定范围内的属性表达的构件对应的手指构件位于基部构件的上部，并且与具有 不在范围内的属性的表达位于基础成员的下部。 用于形成恒星图标的方法和系统包括形成包括关于要表示的范围和组成和属性的数据的变换表（47）。 变换表用于确定每个指状构件在基底构件上的相对位置并确定手指构件的长度，使得长度对应于该属性的表达。

公开(公告)号：WO2003012418A1

公开(公告)日：2003-02-13

申请号：PCT/US2002/023664

申请日：2002-07-25

Applicant: COULTER INTERNATIONAL CORP.

Inventor： TAYLOR, Richard, Lee ,  ZHENG, Min

IPC: G01N27/00

CPC classification number: G01N15/12 ,  G01N2015/025 ,  G01N2015/1062

Abstract: A method is disclosed which allows more accurate counting of particles where the sample has significant size variability between particles. The method uses flight time (44) and wait time (22) to obtain a corrected count of particles (12).

Abstract translation: 公开了一种方法，其允许对颗粒进行更精确的计数，其中样品在颗粒之间具有显着的尺寸变化。 该方法使用飞行时间（44）和等待时间（22）获得校正的粒子数（12）。