公开(公告)号：WO2011091436A9

公开(公告)日：2011-09-15

申请号：PCT/US2011022454

申请日：2011-01-25

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  DEY SUBHAKAR ,  PILLAI SASI ,  WILLIAMSON BRIAN ,  PURKAYASTHA SUBHASISH

Inventor： DEY SUBHAKAR ,  PILLAI SASI ,  WILLIAMSON BRIAN ,  PURKAYASTHA SUBHASISH

IPC: A01N45/00 ,  A61K31/41 ,  A61K31/59

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled Diels Alder reagent.

Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的Diels Alder试剂的一步标记试剂。

公开(公告)号：WO2011138669A3

公开(公告)日：2011-12-29

申请号：PCT/IB2011000972

申请日：2011-05-06

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  ALBEANU NICOLAE ,  DIMA MARTIAN

Inventor： ALBEANU NICOLAE ,  DIMA MARTIAN

IPC: H01J49/40

CPC classification number: H01J49/16 ,  H01J49/0031 ,  H01J49/0095 ,  H01J49/06 ,  H01J49/401 ,  H01J49/403

Abstract: There is provided a pulser, a time of flight mass spectrometer system comprising the same, and a method of analyzing the ions using the pulser. The pulser comprises a first positive switch for coupling and decoupling a first electrode of the accelerator assembly to a first positive voltage; a first negative switch for coupling and decoupling the first electrode to a first negative voltage; and, a first bipolar switch for alternately coupling and decoupling the first electrode to a third voltage.

Abstract translation: 提供了一种脉冲发生器，包括该脉冲发生器的飞行时间质谱仪系统和使用脉冲发生器分析离子的方法。 脉冲发生器包括用于将加速器组件的第一电极耦合和解耦到第一正电压的第一正开关; 用于将第一电极耦合和去耦合到第一负电压的第一负开关; 以及用于交替地将第一电极耦合和去耦合到第三电压的第一双极开关。

公开(公告)号：WO2017025893A3

公开(公告)日：2017-05-26

申请号：PCT/IB2016054777

申请日：2016-08-09

Applicant: DH TECHNOLOGIES DEV PTE LTD

Inventor： BURTON LYLE LORRENCE ,  COX DAVID MICHAEL

IPC: H01J49/00

CPC classification number: H01J49/0036 ,  H01J49/004

Abstract: One or more known compounds of a sample are ionized using an ion source, producing an ion beam of precursor ions. A tandem mass spectrometer receives the ion beam from the ion source, selects one or more precursor ions from the ion beam using a precursor ion mass selection window, fragments precursor ions within the precursor ion mass selection window, and mass analyzes the resulting product ions, producing an unknown product ion mass spectrum. A library product ion mass spectrum for a known compound is retrieved from a memory. Each peak of the unknown spectrum is analyzed for a potential non- halogen isotopic peak using a processor, and if a potential non-halogen isotopic peak is found, it is removed if it does not have a corresponding peak in the library spectrum.

Abstract translation: 使用离子源将一种或多种已知的样品化合物离子化，产生前体离子的离子束。 串联质谱仪接收来自离子源的离子束，使用前体离子质量选择窗口从离子束中选择一个或多个前体离子，在前体离子质量选择窗口内分裂前体离子，并质量分析所得产物离子， 产生未知的产物离子质谱。 从存储器中检索已知化合物的文库产物离子质谱。 使用处理器分析未知光谱的每个峰的潜在非卤素同位素峰，如果找到潜在的非卤素同位素峰，则如果其在谱库谱中没有相应的峰，则将其去除。

公开(公告)号：WO2013008086A3

公开(公告)日：2013-03-14

申请号：PCT/IB2012001366

申请日：2012-07-11

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  GUNA MIRCEA

Inventor： GUNA MIRCEA

IPC: H01J49/26

CPC classification number: H01J49/4265 ,  H01J49/004 ,  H01J49/422 ,  H01J49/427

Abstract: A method for operating a mass spectrometer having an ion trap over a plurality of selected mass-to-charge ranges constituting an overall mass- to-charge range is disclosed. For each of the plurality of selected mass-to- charge ranges the method comprises filling the ion trap with fragmented ions of the selected mass-to-charge ranges, cooling the fragmented ions trapped in the ion trap for a first cooling period, applying an RF voltage and a resolving direct current voltage to the ion trap for eliminating any remaining fragmented ions outside the selected ion mass-to-charge range and retaining ions within the selected ion mass-to-charge range, cooling the retained ions in the ion trap for a second cooling period, and scanning the retained ions out of the ion trap and detecting the ions released therefrom.

Abstract translation: 公开了一种在构成总质量 - 电荷范围的多个选择的质量 - 电荷范围内操作具有离子阱的质谱仪的方法。 对于多个选择的质量 - 电荷范围中的每一个，所述方法包括用选定的质量 - 电荷范围的碎片离子填充离子阱，冷却在第一冷却期间捕集在离子阱中的碎裂离子， RF电压和分离到离子阱的直流电压，用于消除选定的离子质量 - 电荷范围之外的任何剩余的分散离子，并将离子保持在所选离子质量 - 电荷范围内，冷却离子阱中的保留离子 在第二冷却期间，并将残留的离子从离子阱中扫描出来并检测从其释放的离子。

公开(公告)号：WO2012135682A2

公开(公告)日：2012-10-04

申请号：PCT/US2012031553

申请日：2012-03-30

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  DEY SUBHAKAR N ,  MINKOFF MARJORIE S ,  PILLAI SASI K ,  PURKAYASTHA SUBHASISH ,  WILLIAMSON BRIAN L

IPC: H01J49/04 ,  G01N27/62

CPC classification number: H01J49/0009 ,  H01J49/0027

Abstract: A composition, method, and kit for calibrating a mass spectrometer including a predetermined concentration of a calibrant having a mixture of amino acid polyethylene glycol compounds and a solvent for dissolving the calibrant. The calibrant can be used in either positive or negative ionization mode, and it can be used in calibrating an atmospheric pressure chemical ionization or an electrospray mass spectrometer. The calibrant can include a mixture to enable calibration across a broad range of masses.

Abstract translation: 用于校准质谱仪的组合物，方法和试剂盒，其包括具有氨基酸聚乙二醇化合物和用于溶解校准物的溶剂的混合物的校准物的预定浓度。 校准液可用于正离子或负离子模式，可用于校准大气压化学电离或电喷雾质谱仪。 校准物可以包括混合物以使得能够在宽范围的质量范围内进行校准。

公开(公告)号：WO2012035412A3

公开(公告)日：2012-05-10

申请号：PCT/IB2011002139

申请日：2011-09-14

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  ETH ZUERICH ,  BONNER RONALD ,  TATE STEPHEN ,  AEBERSOLD RUDOLF ,  NAVARRO ALVAREZ PEDRO JOSE ,  RINNER OLIVER ,  REITER LUKAS ,  GILLET LUDOVIC

Inventor： BONNER RONALD ,  TATE STEPHEN ,  AEBERSOLD RUDOLF ,  NAVARRO ALVAREZ PEDRO JOSE ,  RINNER OLIVER ,  REITER LUKAS ,  GILLET LUDOVIC

IPC: H01J49/00

CPC classification number: H01J49/0036 ,  G01N30/7233 ,  G06F19/703 ,  G06F19/709 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/0045 ,  H01J49/42

Abstract: Systems and methods are used to store an electronic record of all product ion spectra of all detectable compounds of a sample. A plurality of product ion scans are performed on a tandem mass spectrometer one or more times in a single sample analysis across a mass range using a plurality of mass selection windows. All sample product ion spectra of all detectable compounds for each mass selection window are produced. All sample product ion spectra for each mass selection window are received from the tandem mass spectrometer using a processor. All sample product ion spectra for each mass selection window are stored as an electronic record of all detectable compounds of the sample using the processor. The electronic record is used to characterize compounds known at the time the electronic record is stored or to characterize compounds that became known after the electronic record was stored.

Abstract translation: 系统和方法用于存储样品中所有可检测化合物的所有产物离子谱的电子记录。 使用多个质量选择窗在整个质量范围的单个样品分析中在串联质谱仪上执行一次或多次多次产物离子扫描。 生成每个质量选择窗口中所有可检测化合物的所有样品产物离子谱图。 使用处理器从串联质谱仪接收每个质量选择窗口的所有样品产物离子质谱图。 使用处理器将每个质量选择窗口的所有样品产物离子谱存储为样品中所有可检测化合物的电子记录。 电子记录用于表征电子记录存储时已知的化合物的特征，或描述电子记录存储后已知的化合物的特征。

公开(公告)号：WO2012032394A3

公开(公告)日：2012-05-10

申请号：PCT/IB2011002057

申请日：2011-09-07

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  BONNER RONALD ,  TATE STEPHEN A

Inventor： BONNER RONALD ,  TATE STEPHEN A

IPC: H01J49/00

CPC classification number: H01J49/0081 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/0045

Abstract: Systems and methods are used to analyze a sample using variable mass selection window widths. A tandem mass spectrometer is instructed to perform at least two fragmentation scans of a sample with different mass selection window widths using a processor. The tandem mass spectrometer includes a mass analyzer that allows variable mass selection window widths. The selection of the different mass selection window widths can be based on one or more properties of sample compounds. The properties may include a sample compound molecular weight distribution that is calculated from a molecular weight distribution of expected compounds or is determined from a list of molecular weights for one or more known compounds. The tandem mass spectrometer can also be instructed to perform an analysis of the sample before instructing the tandem mass spectrometer to perform the at least two fragmentation scans of the sample.

Abstract translation: 系统和方法用于使用可变质量选择窗口宽度来分析样本。 指示串联质谱仪使用处理器对具有不同质量选择窗口宽度的样品进行至少两次碎片扫描。 串联质谱仪包括质量分析器，允许质量选择窗口宽度可变。 不同质量选择窗口宽度的选择可以基于样品化合物的一种或多种性质。 性质可以包括根据预期化合物的分子量分布计算的样品化合物分子量分布，或由一种或多种已知化合物的分子量列表确定。 也可以指示串联质谱仪在指示串联质谱仪进行样品的至少两次碎片扫描之前对样品进行分析。

公开(公告)号：WO2012023031A3

公开(公告)日：2012-04-26

申请号：PCT/IB2011001905

申请日：2011-08-18

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  GUNA MIRCEA ,  COLLINGS BRUCE

Inventor： GUNA MIRCEA ,  COLLINGS BRUCE

IPC: H01J49/02

CPC classification number: H01J49/0031 ,  H01J49/025 ,  H01J49/06 ,  H01J49/061

Abstract: A mass spectrometer system can include a mass analyzer operable to mass transmit streams of ions to a detector in a mass dependent fashion for measurement of ion flux intensity. An ion attenuator can be located in the extraction region between the mass analyzer and detector, downstream of the mass analyzer, and can be operable to provide selective attenuation of the ion beam by attenuating ion flux intensity also in mass dependent fashion. Higher concentration ions can be selected and attenuated, while other lower concentration ions can be left unattenuated. Different ions can be attenuated to different degrees. Locating the ion attenuator downstream of the mass analyzer so that the ion beam is already mass differentiated when attenuated can avoid mass discriminatory effects associated with ion beam attenuators. Selective attenuation of only certain ions but not others can extend the dynamic range of the detector without necessarily sacrificing detector sensitivity.

Abstract translation: 质谱仪系统可以包括质量分析器，可以以质量依赖的方式将离子流质量传递到检测器，以测量离子通量强度。 离子衰减器可以位于质量分析器和检测器之间的质量分析器下游的提取区域中，并且可以通过以质量依赖的方式衰减离子通量强度来提供离子束的选择性衰减。 可以选择和减弱较高浓度的离子，而其他较低浓度的离子可以保持不衰减。 不同的离子可以衰减到不同的程度。 将质子分析仪下游的离子衰减器定位，使得当衰减时离子束已经被质量区分，可以避免与离子束衰减器相关的质量鉴别效应。 只有某些离子而不是其他离子的选择性衰减才能扩展检测器的动态范围，而不必牺牲检测器灵敏度。

公开(公告)号：WO2012025821A3

公开(公告)日：2012-04-19

申请号：PCT/IB2011001951

申请日：2011-08-25

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  GUNA MIRCEA

Inventor： GUNA MIRCEA

IPC: H01J49/42

CPC classification number: H01J49/4285 ,  H01J49/422 ,  H01J49/4225

Abstract: A system and method involving processing ions in a linear ion trap are provided, involving a two-dimensional asymmetric substantially quadrupole field having a hexapole and octopole component.

Abstract translation: 提供了涉及在线性离子阱中处理离子的系统和方法，涉及具有六极和八极组分的二维非对称基本四极场。

公开(公告)号：WO2012028940A2

公开(公告)日：2012-03-08

申请号：PCT/IB2011002011

申请日：2011-09-01

Applicant: DH TECHNOLOGIES DEV PTE LTD ,  KOVARIK PETER ,  COVEY THOMAS R

Inventor： KOVARIK PETER ,  COVEY THOMAS R

CPC classification number: H01J49/0422 ,  H01J49/04

Abstract: Systems and methods for delivering a sample to a mass spectrometer are provided. In one aspect, the system can include a sample source for generating a sample plume entrained in a primary gas stream in a first flow direction at a first flow rate, and a gas source for generating a secondary gas stream along a second flow direction different from the first flow direction and at a second flow rate greater than the first flow rate. The sample source and the gas source can be positioned relative to one another such that the primary gas stream intersects the secondary gas stream so as to generate a resultant gas stream propagating along a trajectory different from said first and second direction to bring the sample to proximity of a sampling orifice of the mass spectrometer.

Abstract translation: 提供了将样品递送到质谱仪的系统和方法。 在一个方面，系统可以包括用于产生以第一流量在第一流动方向上夹带在主气流中的样品羽流的样品源，以及用于沿着不同于第二流动方向的第二流动方向产生二次气流的气体源 第一流动方向和大于第一流量的第二流量。 样品源和气体源可以相对于彼此定位，使得主气体流与次级气流相交，以便产生沿着与所述第一和第二方向不同的轨迹传播的所得气流，以使样品接近 的质谱仪的采样孔。