公开(公告)号：WO1997023602A1

公开(公告)日：1997-07-03

申请号：PCT/EP1996005812

申请日：1996-12-19

Applicant: SOCIETE DES PRODUITS NESTLE S.A. ,  BAUR, Markus ,  MACE, Catherine ,  MALNOE, Armand ,  PFEIFER, Andrea, M., A. ,  REGNIER, Marcelle

Inventor： SOCIETE DES PRODUITS NESTLE S.A.

IPC: C12N05/08

CPC classification number: C12N5/0629 ,  A61K35/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/39 ,  C12N2501/81 ,  C12N2501/999 ,  C12N2510/04

Abstract: The present invention relates to improved continuous (immortalized) cell lines, in particular keratinocytes and melanocytes derived from normal human skin tissue. The present invention also relates to novel serum-free media for isolating, producing and maintaining said improved continuous keratinocyte and melanocyte cell lines. The present invention also relates to methods for producing primary melanocytes and keratinocytes under serum-free conditions without any feeder cells.

Abstract translation: 本发明涉及改进的连续（永生化）细胞系，特别是衍生自正常人皮肤组织的角质形成细胞和黑素细胞。 本发明还涉及用于分离，产生和维持所述改进的连续角质形成细胞和黑素细胞细胞系的新型无血清培养基。 本发明还涉及在无任何饲养细胞的无血清条件下生产原代黑素细胞和角质形成细胞的方法。

公开(公告)号：WO2016007841A1

公开(公告)日：2016-01-14

申请号：PCT/US2015/039917

申请日：2015-07-10

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： SIA, Samuel ,  GILLETTE, Brian

IPC: A61K38/00 ,  A01N63/00 ,  C12N5/00

CPC classification number: C12N5/0653 ,  A61K35/35 ,  A61K35/44 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/80 ,  C12N2501/81 ,  C12N2501/999 ,  C12N2523/00 ,  A61K2300/00

Abstract: Provided are methods, apparatus, pharmaceutical compositions, and kits for treatment of a metabolic condition, including obesity and type 2 diabetes, by administration to a subject of a therapeutically effective amount of a cell or tissue preparation such as brown adipose microtissues or brown adipose tissue directly converted from white adipose tissue. Modified approaches to creating brown adipose tissue involve differentiation of explanted white adipose tissue and direct browning of white adipose tissue in a bioreactor rather than isolation and expansion of adipose stems cells or endothelial cells and formation and differentiation of 3D cell aggregates.

Abstract translation: 提供了通过向受试者施用治疗有效量的细胞或组织制剂如棕色脂肪微量组织或棕色脂肪组织来治疗代谢病症（包括肥胖症和2型糖尿病）的方法，装置，药物组合物和试剂盒 从白色脂肪组织直接转化。 创建棕色脂肪组织的修改方法包括移植的白色脂肪组织的分化和生物反应器中白色脂肪组织的直接褐变，而不是脂肪干细胞或内皮细胞的分离和扩增以及3D细胞聚集体的形成和分化。

公开(公告)号：WO2015196012A1

公开(公告)日：2015-12-23

申请号：PCT/US2015/036577

申请日：2015-06-19

Applicant: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY

Inventor： SABAAWY, Hatem ,  BARTUCCI, Monica

IPC: C12N5/071

CPC classification number: C12N5/0693 ,  A01K67/0278 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2227/105 ,  A01K2267/0331 ,  C12N5/0683 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/84 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2501/81 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/03 ,  C12N2513/00 ,  G01N33/5011 ,  G01N33/5082

Abstract: The present invention relates to organoids derived from a single cell, such as a prostate cancer cell, and methods and compositions relating to the production and use thereof, including cell culture medium for producing organoids and methods of personalized treatment for prostate cancer. The invention further provides a humanized mouse comprising a prostate organoid derived from a patient's prostate cell.

Abstract translation: 本发明涉及衍生自单细胞如前列腺癌细胞的组织细胞，以及与其生产和使用有关的方法和组合物，包括用于生产组织的细胞培养基和用于前列腺癌的个性化治疗方法。 本发明还提供了包含源自患者前列腺细胞的前列腺组织细胞的人源化小鼠。

公开(公告)号：WO2009156413A1

公开(公告)日：2009-12-30

申请号：PCT/EP2009/057852

申请日：2009-06-23

Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  AILHAUD, Gérard, Paul ,  AMRI, Ez-Zoubir ,  DANI, Christian, Jean, Lucien ,  ELABD, Christian

Inventor： AILHAUD, Gérard, Paul ,  AMRI, Ez-Zoubir ,  DANI, Christian, Jean, Lucien ,  ELABD, Christian

IPC: C12N5/08 ,  G01N33/50

CPC classification number: C12N5/0667 ,  C12N2501/115 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/81 ,  G01N33/5044

Abstract: L'invention a pour objet une population d'adipocytes bruns humains fonctionnelle, dans laquelle l'expression de UCP1, CIDEA, CPT1B et Bc12 est supérieure, l'expression de Bax est inférieure, et l'expression de PPAR alpha, PGC1 alpha, PGC1 bêta et PRDM16 est similaire par rapport aux expressions correspondantes d'une population d'adipocytes blancs humains. L'invention concerne également un procédé de différenciation de cellules hMADS en la population d'adipocytes bruns humains fonctionnelle, un procédé de conversion d'une population d'adipocytes blancs humains en la population d'adipocytes bruns humains fonctionnelle, ainsi qu'une méthode de criblage de molécules susceptibles de moduler le poids corporel chez un sujet.

Abstract translation: 本发明的主题是人类棕色脂肪细胞的功能群体，其中UCP1，CIDEA，CPT1B和Bc12的表达较高，Bax的表达较低，PPAR-α，PGC-1α，PGC- 1beta和PRDM16与人类白色脂肪细胞群体的相应表达相似。 本发明还涉及将hMADS细胞分化为人类棕色脂肪细胞的功能群体的方法，将人白色脂肪细胞群转化为人类棕色脂肪细胞的功能群体的方法，以及筛选 能够调节个体体重的分子。

公开(公告)号：WO2007148332A1

公开(公告)日：2007-12-27

申请号：PCT/IL2007/000741

申请日：2007-06-19

Applicant: YEDA RESAERCH AND DEVELOPMENT CO. LTD ,  LAPIDOT, Tsvee ,  SPIEGEL, Asaf ,  RUBINSTEIN, Menachem ,  KALINKOVICH, Alexander ,  SHIVTIEL, Shoham

Inventor： LAPIDOT, Tsvee ,  SPIEGEL, Asaf ,  RUBINSTEIN, Menachem ,  KALINKOVICH, Alexander ,  SHIVTIEL, Shoham

IPC: A61K33/00 ,  A61K35/00

CPC classification number: A61K45/06 ,  A61K31/137 ,  A61K31/428 ,  A61K31/473 ,  A61K31/496 ,  A61K31/55 ,  A61K35/28 ,  A61K38/1787 ,  A61K38/193 ,  C12N5/0647 ,  C12N2501/22 ,  C12N2501/80 ,  C12N2501/81 ,  C12N2501/815 ,  A61K2300/00

Abstract: The invention relates to receptors of catecholamines and their role in stem cell development and function.

Abstract translation: 本发明涉及儿茶酚胺的受体及其在干细胞发育和功能中的作用。

公开(公告)号：WO2005068614A3

公开(公告)日：2005-11-03

申请号：PCT/NL2005000026

申请日：2005-01-14

Applicant: VERENIGING VOOR CHRISTELIJK WETENSCHAPPELIJK ONDERWIJS ,  GIBBS SUSAN ,  DE BOER EDITH MARGARETHA ,  KIRTSCHIG GUDULA ,  SCHEPER RIEKELD JOHANNES ,  BRUYNZEEL DERK PIETER

Inventor： GIBBS SUSAN ,  DE BOER EDITH MARGARETHA ,  KIRTSCHIG GUDULA ,  SCHEPER RIEKELD JOHANNES ,  BRUYNZEEL DERK PIETER

IPC: A61K35/12 ,  C12N5/071 ,  C12N5/08 ,  A61P17/02 ,  C12N5/06

CPC classification number: C12N5/0698 ,  A61K35/12 ,  C12N2500/33 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/81 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/92

Abstract: Disclosed is a method for in vitro growing of connective tissue substitute, said connective tissue substitute being populated with fibroblasts, a connective tissue substitute obtainable by such a method, as well as a method for closing of a wound, wherein such connective tissue substitute is applied onto a wound.

Abstract translation: 公开了一种用于结缔组织替代物的体外生长的方法，所述结缔组织替代物填充有成纤维细胞，通过这种方法获得的结缔组织替代物以及关闭伤口的方法，其中应用这种结缔组织替代物 上伤口。

公开(公告)号：WO9413260A3

公开(公告)日：1996-06-13

申请号：PCT/FR9301225

申请日：1993-12-10

Applicant: LMVH RECH ,  BONTE FREDERIC ,  MEYBECK ALAIN ,  DUMAS MARC

Inventor： BONTE FREDERIC ,  MEYBECK ALAIN ,  DUMAS MARC

IPC: A61K8/00 ,  A61K8/14 ,  A61K8/33 ,  A61K8/34 ,  A61K8/44 ,  A61K8/49 ,  A61K8/64 ,  A61K8/73 ,  A61K8/96 ,  A61K8/97 ,  A61K31/047 ,  A61K31/07 ,  A61K31/215 ,  A61K31/375 ,  A61K31/56 ,  A61K36/00 ,  A61K36/18 ,  A61K36/185 ,  A61L27/00 ,  A61P17/00 ,  A61Q5/00 ,  A61Q17/00 ,  A61Q19/00 ,  A61Q19/02 ,  C12N5/00 ,  C12N5/071 ,  A61K7/48 ,  A61K7/06 ,  A61K35/78

CPC classification number: C12N5/0629 ,  A61K8/97 ,  A61K36/185 ,  A61Q5/00 ,  A61Q17/00 ,  A61Q19/02 ,  C12N5/0626 ,  C12N2500/76 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/81 ,  A61K2300/00

Abstract: The use of a simarouba extract (A) to produce (a) cosmetic or pharmaceutical (esp. dermatological) compsns. and (b) culture media for cells and tissues (partic. bulk culture of skin cells, esp. keratinocytes), is new. (A) have (1) depigmenting action and stimulate differentiation of keratinocytes and (2) restore, preserve, and/or reinforce the protective function of the skin (partic. its water-barrier function) cohesion of epidermal cells, and also improve quality of the hair.

Abstract translation: 使用苦参提取物（A）制备（a）化妆品或药物（尤其是皮肤病学）的化合物。 和（b）细胞和组织的培养基（特别是皮肤细胞，特别是角质形成细胞的大量培养物）是新的。 （A）具有（1）脱色作用并刺激角质形成细胞的分化，（2）恢复，保存和/或增强表皮细胞的保护功能（特别是其阻水功能）凝聚力，并提高质量 的头发。

公开(公告)号：WO1994013260A2

公开(公告)日：1994-06-23

申请号：PCT/FR1993001225

申请日：1993-12-10

Applicant: LMVH RECHERCHE ,  BONTE, Frédéric ,  MEYBECK, Alain ,  DUMAS, Marc

Inventor： LMVH RECHERCHE

IPC: A61K07/48

CPC classification number: C12N5/0629 ,  A61K8/97 ,  A61K36/185 ,  A61Q5/00 ,  A61Q17/00 ,  A61Q19/02 ,  C12N5/0626 ,  C12N2500/76 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/81 ,  A61K2300/00

Abstract: A simarouba extract is used to produce a cosmetic or pharmaceutical, and particularly dermatological, composition or a skin cell culture medium. The resulting compositions are also disclosed. The simarouba extract has a significant skin depigmentation activity and can enhance the protective function of the skin, particularly its water barrier function, as well as having a significant keratinocyte differentiation activity.

Abstract translation: 西马鲁巴提取物用于制备化妆品或药物，特别是皮肤病学的组合物或皮肤细胞培养基。 还公开了所得组合物。 辛伐他丁提取物具有显着的皮肤色素沉着活性，并且可以增强皮肤的保护功能，特别是其水屏障功能，以及具有显着的角质化细胞分化活性。

公开(公告)号：WO2013060899A2

公开(公告)日：2013-05-02

申请号：PCT/EP2012071418

申请日：2012-10-29

Applicant: UNIV LEIPZIG ,  SAVKOVIC VUK ,  DIECKMANN CHRISTINA ,  SIMON JAN-CHRISTOPH ,  SCHULZ-SIEGMUND MICHAELA ,  HACKER MICHAEL

Inventor： SAVKOVIC VUK ,  DIECKMANN CHRISTINA ,  SIMON JAN-CHRISTOPH ,  SCHULZ-SIEGMUND MICHAELA ,  HACKER MICHAEL

IPC: C12N5/071 ,  A61L27/36

CPC classification number: A61L27/3834 ,  A61L27/3895 ,  A61L27/60 ,  C12N5/0626 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/33 ,  C12N2501/81 ,  C12N2506/03 ,  C12N2533/40

Abstract: The present invention relates to a method for generating melanocytes from stem cells comprising the steps of: (i) removing the bulb of an epilated human hair; (ii) incubating the remaining part of the epilated hair with a collagen degrading agent to separate stem cells from the outer root sheath; (iii) cultivating the separated stem cells from step (ii) in a medium that induces differentiation and stimulates growth of stem cells, melanocyte precursors and melanocytes, wherein the medium comprises one or more growth factors for differentiation into melanotic melanocytes. Furthermore, a method for producing an autograft, a homograft or an allograft comprising melanocytes is disclosed herein as well as autografts, a homografts and allografts or melanocytes for the treatment of diseases related to depigmentation of the skin or for the treatment of scars.

Abstract translation: 本发明涉及一种从干细胞产生黑素细胞的方法，包括以下步骤：（i）去除脱毛的人毛发的球; （ii）将脱毛毛发的剩余部分用胶原降解剂孵育以从外根鞘分离干细胞; （iii）在诱导分化并刺激干细胞，黑素细胞前体和黑素细胞生长的培养基中培养来自步骤（ii）的分离的干细胞，其中所述培养基包含用于分化成黑素细胞黑素细胞的一种或多种生长因子。 此外，本文公开了一种用于生产包含黑素细胞的自体移植物，同种移植物或同种异体移植物的方法，以及自体移植物，同种移植物和同种异体移植物或黑素细胞，用于治疗与皮肤脱色素或治疗疤痕有关的疾病。

公开(公告)号：WO2010138782A1

公开(公告)日：2010-12-02

申请号：PCT/US2010/036505

申请日：2010-05-28

Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH ,  HICKMAN, James, J. ,  DAVIS, Hedvika

Inventor： HICKMAN, James, J. ,  DAVIS, Hedvika

IPC: A01N63/00 ,  C12N5/071

CPC classification number: C12N5/0622 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/70 ,  C12N2501/81 ,  C12N2501/91 ,  C12N2506/025 ,  C12N2506/1369 ,  C12N2506/1392

Abstract: The invention provides a method of producing oligodendrocytes by in vitro differentiation of human multi-potent progenitor cells (MLPCs). The method comprises culturing culturing isolated MLPCs on a first surface in a serum-free defined culture medium; replacing the culture medium with serum-free culture medium supplemented with bFGF, EGF and PDGF-AA for approximately 24 hours; changing the cultured MLPCs into the supplemented serum-free culture medium further supplemented with differentiation factors norepinephrine, forskolin. and K252a; establishing a 3D environment by covering the culture with a second surface opposite and spaced apart from the first surface, so as to contain the MLPCs therebetween; and continuing to culture until a majority of the MLPCs have differentiated into oligodendrocytes. Additionally included is a method of treatment for a subject afflicted by a disease characterized by central or peripheral nervous system demyelination, the method comprising transplanting into the subject oligodendrocytes produced according to the method disclosed.

Abstract translation: 本发明提供了通过体外分化人多能祖细胞（MLPC）产生少突胶质细胞的方法。 该方法包括在无血清定义培养基的第一表面培养培养分离的MLPC; 用补充有bFGF，EGF和PDGF-AA的无血清培养基代替培养基约24小时; 将培养的MLPC变成补充的无血清培养基，进一步补充分化因子去甲肾上腺素，毛喉素。 和K252a; 通过用与第一表面相对且间隔开的第二表面覆盖培养来建立3D环境，以便在其间容纳MLPC; 并继续培养，直到大多数MLPC已经分化为少突胶质细胞。 另外包括治疗由以中枢神经系统或周围神经系统脱髓鞘为特征的疾病所折磨的受试者的方法，该方法包括将根据所公开的方法产生的少突胶质细胞移植到受试者中。