Abstract:
Disclosed is a method for discovering pharmacologically active substances from natural products at high speed, including: obtaining an activity profile by testing pharmacological activity of a plurality of samples; obtaining a mass profile based on a mass spectrum resulting from analysis of the samples by mass spectrometry; and determining molecular weight of pharmacologically active substances by comparing and analyzing the activity profile and the mass profile. The disclosed method allows fast discovery of pharmacologically active substances by performing high resolution mass spectrometry for numerous components included in an extract sample of natural products and comparing with the activity test data. The information about the intensity of the activity of the pharmacologically active substances of the natural products allows effective utilization of the natural products.
Abstract:
An ion chromatography housing for easy insertion and removal of a plurality of component cartridges is disclosed. Various components of the IC system are provided in the separate component cartridges. The IC housing includes a capillary separation column and may be connected to conventional-scale components of an IC system. A plurality of IC housings may be provided in a compartment with one or more separation columns. The columns may be capillary columns or conventional-scale columns. A method of using the ion chromatography system is also disclosed. The IC system may be utilized to perform two-dimensional ion chromatographic separation.
Abstract:
Methods for regenerating acidic ion-exchange resins and reusing regenerants in such methods are provided. A spent ion-exchange resin is contacted with an alcohol ion-exchange regenerant. The spent ion-exchange resin is thereafter contacted with an acidic ion-exchange regenerant to recharge the acidic ion-exchange resin to produce a regenerated acidic ion-exchange resin. Metal- and water-containing biomass-derived pyrolysis oil is then contacted with the regenerated acidic ion-exchange resin to produce low metal, water-containing biomass-derived pyrolysis oil. The regenerated acidic ion-exchange resin may be recycled. The spent alcohol and acid ion-exchange regenerants may be recovered and recycled.
Abstract:
A separation or reaction unit (1; 1'; 81; 81'; 101) and a method for aseptically connecting such units. The separation or reaction unit (1; 1'; 81; 81'; 101) comprises at least one fluid inlet (3a, 3b, 5a, 5b; 3a', 3b', 5a', 5b'; 85a, 85b; 103a, 103b) and at least one fluid outlet (3a, 3b, 5a, 5b; 3a', 3b', 5a', 5b'; 85a, 85b; 103a, 103b). At least one of the inlet or outlet is sealed by at least one film (7, 9; 11; 87a, 87b; 107a, 107b) and the contact surface between the film and the separation or reaction unit is aseptic. The films are adapted to be mated with a corresponding film on another separation or reaction unit or on a fluid distribution unit (20; 57; 61) which the separation or reaction unit possibly should be connected with and said mated films are adapted to be pulled out together two and two after mating such that corresponding fluid inlets/outlets on the two connected units are mated aseptically.
Abstract:
Ion transport apparatus (e.g. an electrolytic eluent generator or a suppressor for ion chromatography) in which ions in a first chamber are transported to a liquid in second chamber through a wall comprising an ion exchange bead sealed in a bead seat. The wall is capable of transport ions but of substantially blocking bulk liquid flow.
Abstract:
Non-suppressed ion chromatography apparatus including a chromatographic column (18), a source (10) of an aqueous eluent liquid stream (12), a detector (20), a recycle line (22,26) between the detector and the chromatographic columninlet, and a purifying device (24) disposed along the recycle line including ion exchange removal medium. Also, such apparatus with an electrolytic purifying device disposed along the recycle line. Also, methods of using such apparatus.
Abstract:
A chromatographic method including chromatographically separating sample ionic species in an eluent stream, detecting the separated sample ionic species, catalytically combining hydrogen and oxygen gases or catalytically decomposing hydrogen peroxide in a catalytic gas elimination chamber (31), and recycling the effluent stream from the catalytic gas elimination chamber to the chromatography separation column (10). The residence time between the detector (14) and said chamber (31) is at least one minute to facilitate decomposition of unstable oxidative compounds. Also, flowing the recycle sequentially through two detector effluent flow channels of an electrolytic membrane suppressor (28). Also, applying heat or UV energy between the detector (14) and the chamber (31). Also, detecting bubbles after the chamber. Also, a Platinum group metal catalyst and ion exchange medium in the chamber. Apparatus for performing the methods.
Abstract:
A displacement chromatography process and displacer compounds used in the process and having the general formula (I), wherein: each group R 1 , R 2 , R 3 , R’ 1 , R' 2 , and R’ 3 , independently may be selected from alkyl, aryl, and aralkyl, and in which a ring containing one or more quaternary nitrogen may be formed by any one or more of R 1 and R 2 , R 1 and R' 1 , R 1 and R 4 , R 4 and R' 4 , or R 4 and R 5 ; each R 4 , R' 4 , R 5 and R' 5 independently may be selected from alkyl, aryl, aralkyl and -(CH 2 ) a -(CHY) b -(CH 2 ) c -N + R 1 R 2 R 3 An¯, wherein R 1 , R 2 and R 3 are as defined above; each Y independently may be selected from -H, -OH, -OR 6 , halo, alkyl, aryl and aralkyl, wherein -R 6 may be alkyl or -(CH 2 ) a -(CHOH) b -(CH 2 ) c -N + R 1 R 2 R 3 An¯ , wherein R 1 , R 2 and R 3 are as defined above; each q and z independently may be any whole number from 0 to about 6, with the proviso that q + z is equal to or less than about 6; each a, b, and c independently may be any whole number from 0 to 2, with the proviso that the sum a+b+c in any fragment is at least 1; and each An¯ independently may be one or more organic or inorganic, monovalent or polyvalent anion as needed to obtain a neutral compound.
Abstract:
An ion chromatography system including sample preparation. The system includesa liquid sample injection loop (24), an ion concentrator (32), an ion separator (40) and only a single pump (20) for pumping fluid through the system.
Abstract:
Systems and methods for monitoring analytes in real time using integrated chromatography systems and devices. Integrated microfluidic liquid chromatography devices and systems include multiple separation columns (110, 114, 115, 116) integrated into a single substrate. Using such a device, parallel analysis of multiple samples can be performed simultaneously and/or sequential analysis of a single sample can be performed simultaneously and/or sequential analysis of a single sample can be performed simultaneously on a single chip or substrate. The devices and systems are well suited for use in high pressure liquid chromatography (HPLC) applications. HPLC chips and devices including embedded parylene channels can be fabricated using a single mask process.