公开(公告)号：WO2021148488A2

公开(公告)日：2021-07-29

申请号：PCT/EP2021/051232

申请日：2021-01-21

Applicant: F. HOFFMANN-LA ROCHE AG ,  ROCHE DIAGNOSTICS GMBH ,  ROCHE SEQUENCING SOLUTIONS, INC.

Inventor： ASTIER, Yann ,  BURGESS, Daniel ,  SCHLECHT, Ulrich ,  YANG, Jaeyoung

IPC: B01L3/00 ,  C07K16/00 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0848 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L3/502761 ,  C12Q1/68

Abstract: The present disclosure is directed to automated systems including a microfluidic chip having one or more independently operable processing conduits. In some embodiments, the automated systems are suitable for use in sample cleanup and/or target enrichment processes, such as sample cleanup and/or target enrichment processes conducted prior to sequencing.

公开(公告)号：WO2023057345A1

公开(公告)日：2023-04-13

申请号：PCT/EP2022/077363

申请日：2022-09-30

Applicant: DEPIXUS

Inventor： BAI, Hua

IPC: G01N27/327 ,  B01L3/00 ,  C12Q1/6825 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0893 ,  B01L2400/0415 ,  B01L3/50853 ,  G01N27/3276 ,  G01N27/3278

Abstract: An apparatus for biomolecule analysis, comprising a plurality of cells (1) comprising a well (10) extending along the axis (X) from a top surface (4) towards a bottom surface (2), a molecule (24) anchored to the bottom surface (2) and a bead (22) within the well; an electrically conductive solution filling the well (10); a top electrode (14) and bottom electrode (18) at the bottom surface (2) in contact with the electrically conductive solution, wherein a cavity (20) wider than the well (10) is arranged between the bottom electrode (18) and the well (10), said cavity (20) in fluid communication with the well (10) and exposing the bottom electrode (18) at the bottom surface to the electrically conductive solution filling the cavity (20).

公开(公告)号：WO2023274997A1

公开(公告)日：2023-01-05

申请号：PCT/EP2022/067647

申请日：2022-06-28

Applicant: ETH ZURICH

Inventor： CAO, Xiaobao ,  XUE, Peng ,  AEBERSOLD, Rudolf ,  STAVRAKIS, Stavros ,  DEMELLO, Andrew James

IPC: B01L3/00 ,  G01N33/68 ,  G01N1/40 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/0655 ,  B01L2400/086 ,  B01L3/502738 ,  G01N33/6842

Abstract: In a method for analyzing cells, a sample fluid comprising a suspending medium and cells is fed to a microfluidic device comprising at least one cell processing unit (10) comprising a trapping region (12), a reaction unit (20), and an outlet arrangement (30). The trapping region is delimited by at least an input valve (13) and a sieve valve (14), in particular a v- type valve that is capable of retaining the cells while letting fluids pass. The method comprises trapping cells in the trapping region, subsequently establishing a flow of a reaction fluid through the trapping region while the sieve valve (14) assumes said open state, such that the reaction fluid transfers the trapped cells from the trapping region (12) into the reaction unit (20), decomposing the transferred cells into cell fragments through a decomposition process, collecting the cell fragments in the outlet arrangement, and analyzing the cell fragments.

公开(公告)号：WO2022010356A1

公开(公告)日：2022-01-13

申请号：PCT/NL2021/050440

申请日：2021-07-12

Applicant: LUMICKS CA HOLDING B.V.

Inventor： OSWALD, Felix ,  KAMSMA, Douwe ,  FRANKLIN, Amanda Caroline

IPC: G01N29/036 ,  B01L3/00 ,  G01N29/22 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2400/0436 ,  B01L2400/0439 ,  B01L3/502715 ,  B01L3/502761 ,  G01N15/1463 ,  G01N2015/1075 ,  G01N2291/02466 ,  G01N2291/02809 ,  G01N29/222

Abstract: The disclosure relates to methods and systems for manipulating a particle, in particular for studying small particles and/or biological cellular bodies. A disclosed method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a sample comprising a particle (9) in a fluid medium (11); providing a driving signal, having a signal frequency, a signal amplitude and a signal power, to the sample holder generating an acoustic wave in the sample holder (3) for applying an acoustic force to the particle (9), the wave having a frequency; determining an acoustic resonance frequency of the sample holder and determining a quality factor for the sample holder with respect to the resonance frequency. An associated system (1) is also disclosed.

公开(公告)号：WO2021260666A1

公开(公告)日：2021-12-30

申请号：PCT/IB2021/055726

申请日：2021-06-26

Applicant: STIMSON, William ,  ROTONDO, Dino ,  COWAN, David

Inventor： STIMSON, William ,  ROTONDO, Dino ,  COWAN, David

IPC: G01N35/00 ,  B01L3/00 ,  G01N33/543 ,  B01L2200/0668 ,  B01L2300/044 ,  B01L2300/0663 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L3/50851 ,  B01L3/50853 ,  G01N33/54366 ,  G01N35/00029

Abstract: The present invention relates to the field of assay devices and methods for performing assays, such as immunoassays. The system comprises a means to receive the target sample collected from a subject; a means to measure the target analyte possibly present in said target sample; and a process of measuring the target analytes in real time manner. The target analyte includes but is not limited to any biological analyte, microbial entity like those of viral or bacterial sources such as SARS-CoV-2. The invention thus primarily relates to measuring analytes of interest to detect and treat related indications, such as COVID-19.

公开(公告)号：WO2021211754A2

公开(公告)日：2021-10-21

申请号：PCT/US2021/027347

申请日：2021-04-14

Applicant: QUANTERIX CORPORATION

Inventor： DUFFY, David, C. ,  COLLIER, Nicholas ,  KAN, Cheuk, W. ,  TOBOS, Carmen, I. ,  RISSIN, David, M. ,  WIENER, Alexander, D. ,  MEYER, Ray ,  WARWICK, Christopher ,  MILLINGTON, Roger ,  SVANCARA, Danielle, M.

IPC: B01L3/00 ,  G01N1/40 ,  G01N35/00 ,  G01N35/08 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L3/502761 ,  B01L3/502784 ,  G01N15/0612 ,  G01N15/1456 ,  G01N15/1484 ,  G01N2015/03 ,  G01N2035/0441 ,  G01N35/0098

Abstract: Methods and systems for capture object-based assays, including for determining a measure of the concentration of an analyte molecule or particle in a fluid sample, are described. The methods and systems may relate to high sensitivity detection of analytes, sometimes using assay conditions and sample handling that result in the capture and detection of a high percentage of the analyte molecules or particles in a fluid sample using relatively few capture objects. Apparatuses and methods for immobilizing capture objects with respect to assay sites, in some instances with unexpectedly high efficiencies are also described. Some such apparatuses involve the use of force fields and fluid meniscus forces, alone or in combination, to facilitate or improve capture object immobilization. Also described are techniques for utilizing a relatively high percentage of capture objects in an assay sample, such as by using disclosed sample washing techniques, imaging systems, and analysis procedures that can reduce capture object loss.

公开(公告)号：WO2021097085A2

公开(公告)日：2021-05-20

申请号：PCT/US2020/060227

申请日：2020-11-12

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： GURKAN, Umut ,  MAN, Yuncheng ,  KUCUKAL, Erdem

IPC: B01L3/00 ,  C12N5/071 ,  C07K14/705 ,  A61B5/15 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/086 ,  B01L3/502753 ,  G01N33/49

Abstract: A microfluidic device includes at least one microchannel with a plurality of micropillar arrays provided along a length of the microchannel. Each micropillar array defines a plurality microcapillaries having cross sectional area, and the cross sectional area of the microcapillaries defined by each micropillar array decreases in a direction of fluid flow through the microchannel.

公开(公告)号：WO2020249804A2

公开(公告)日：2020-12-17

申请号：PCT/EP2020/066408

申请日：2020-06-12

Applicant: SHARP LIFE SCIENCE (EU) LIMITED

Inventor： WILSON, Adam Christopher ,  TAYLOR, Peter Neil ,  ANDERSON, Sally ,  ROBERTS, Philip Mark Shryane ,  JACOBS, Adrian Marc Simon ,  PARRY-JONES, Lesley Anne ,  HADWEN, Benjamin James

IPC: B01L3/00 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2400/0427 ,  B01L3/502761 ,  B01L3/502792

Abstract: A method of operating an EWOD device to employs a magnetic field to separate magnetically responsive particles from a polar liquid droplet. The method includes the steps of dispensing a liquid droplet onto an element array of the EWOD device, wherein the liquid droplet includes magnetically responsive particles; performing an electrowetting operation to move the liquid droplet along the element array to a location relative to a magnet element in proximity to that location of the EWOD device; operating the magnet element to apply a magnetic field to the liquid droplet, wherein at least a portion of the magnetically responsive particles aggregate within the liquid droplet in response to the magnetic field; and separating the aggregated magnetically responsive particles from the liquid droplet with the magnetic field, wherein the aggregated magnetically responsive particles move in response to the magnetic field to a location on the element array in proximity to the magnet element. Embodiments of the methods of the present application may be performed by an EWOD control system executing program code stored on a non-transitory computer readable medium.

公开(公告)号：WO2023274566A1

公开(公告)日：2023-01-05

申请号：PCT/EP2021/071437

申请日：2021-07-30

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.

Inventor： ERBEN, Elena ,  MAGHELLI, Nicola ,  KREYSING, Moritz

IPC: B01L3/00 ,  G02B21/32 ,  G06V20/69 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2300/1861 ,  B01L2300/1872 ,  B01L2400/0442 ,  B01L2400/0445 ,  B01L2400/0451 ,  B01L3/502761 ,  G01N15/10 ,  G01N2015/149

Abstract: The invention concerns a method for spatially manipulating at least one particle in a fluid, wherein the particle or the particles is/are spatially manipulated in the fluid by hydrodynamic flows which are generated in the fluid by means of dynamic localized heating of the fluid. The method according to the invention is characterized in that at least one target spatial configuration of the particle(s) in the fluid is defined and that the following further steps are carried out: a) an actual spatial configuration of the particle(s) is captured, b) a specific dynamic localized heating event to be applied to the fluid is determined in dependence of at least one recent actual spatial configuration of the particle(s) and a target configuration of the particle(s), c) the specific dynamic localized heating as determined in step c) is applied at least once to the fluid and d) at least one or all of the steps a) to c) are repeated. The invention concerns furthermore an apparatus for spatially manipulating at least one particle in a fluid by means of hydrodynamic flows a computer program product and a computer-readable storage medium.

公开(公告)号：WO2023274565A1

公开(公告)日：2023-01-05

申请号：PCT/EP2021/071392

申请日：2021-07-30

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.

Inventor： STOEV, Iliya ,  MAGHELLI, Nicola ,  KREYSING, Moritz

IPC: G01N15/10 ,  B01L3/00 ,  G02B21/32 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2200/143 ,  B01L2300/0816 ,  B01L2300/1861 ,  B01L2300/1872 ,  B01L2400/0442 ,  B01L2400/0445 ,  B01L2400/0451 ,  B01L3/502761 ,  G01N2015/149 ,  G06V20/69

Abstract: The invention concerns a method for measuring a force on at least one particle in a fluid wherein an inhomogeneous field of hydrodynamic flows is generated in a fluid by specific dynamic localized heating events, the particle is spatially manipulated by the hydrodynamic flows, a spatial configuration of the particle(s) within the fluid is captured and at least one force acting on the particle(s) is determined by evaluating the captured spatial configuration of the particle(s). The invention concerns furthermore an apparatus for measuring a force on at least one particle in a fluid, a computer program product, and a computer-readable storage medium.