公开(公告)号：WO2023004068A2

公开(公告)日：2023-01-26

申请号：PCT/US2022/037918

申请日：2022-07-21

Applicant: 10X GENOMICS, INC.

Inventor： BOUTET, Stephane, Claude ,  SALMANZADEH, Alireza ,  GIBBONS, Michael

IPC: B01L3/00 ,  C12M1/00 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0854 ,  B01L2400/0409 ,  B01L2400/086 ,  B01L3/5021 ,  B01L3/50273 ,  B01L3/502753

Abstract: Devices, kits, and their methods of use for lysing and/or purifying biological particles, e.g., nuclei are provided. One or more thixotropic layers can be employed in a vessel to purify biological particles. A device with sharp features may be employed to lyse biological particles or the contents thereof.

公开(公告)号：WO2022140344A1

公开(公告)日：2022-06-30

申请号：PCT/US2021/064546

申请日：2021-12-21

Applicant: AMGEN INC.

Inventor： TAN, Glenn, Christopher ,  ZASADZINSKA, Ewelina ,  HOFFMANN, Patrick ,  WU, Bin

IPC: C12N5/00 ,  B01L3/00 ,  C12M1/00 ,  G01N21/64 ,  B01L2200/0668 ,  B01L2300/0896 ,  C12N5/0075

Abstract: The present disclosure provides materials and methods identifying, selecting, and characterizing cells that express and secrete non-Fc containing biomolecules.

公开(公告)号：WO2021253014A1

公开(公告)日：2021-12-16

申请号：PCT/US2021/037297

申请日：2021-06-14

Applicant: BIOFLUIDICA, INC.

Inventor： MULLER, Rolf ,  HUPERT, Mateusz

IPC: B01L3/00 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L3/0275 ,  B01L3/502753

Abstract: The presently disclosed subject matter provides dual-depth thermoplastic microfluidic devices, related kits, microfluidic systems comprising the dual-depth thermoplastic microfluidic device, methods of isolating nucleic acid analytes from a liquid sample, and methods of isolating extracellular vesicles from a liquid sample.

公开(公告)号：WO2021231781A1

公开(公告)日：2021-11-18

申请号：PCT/US2021/032321

申请日：2021-05-13

Applicant: QUIDEL CORPORATION

Inventor： KEHLE, Johannes ,  JACONO, Bruce ,  KROLL, Werner ,  SUR, Kunal ,  PARPIA, Zaheet ,  ELIOPOULOS, Andrew ,  MOROVICH, Matthew ,  MCDANIELS-DAVIDSON, Jesse ,  BJARNASON, Jon ,  WITKOWICKI, Jesse ,  LINHOFF, Todd ,  PACK, Todd Denison

IPC: B01L3/00 ,  G01N35/04 ,  B01L2200/021 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0663 ,  B01L2300/0672 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/5029 ,  B01L3/527 ,  B01L3/561 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/6848 ,  G01N21/55 ,  G01N35/0098

Abstract: A system for analysis of a sample to aid in diagnosis and/or detection of the presence or absence, and/or the identity of, an analyte in the sample is provided. A cartridge with reagents to isolate nucleic acid from a sample inserted in the cartridge and to amplify the isolated nucleic acid, and an instrument that interacts with the cartridge, together provide a self-contained sample to answer system for detection, identification, differentiation and/or quantification of a target nucleic acid in a sample.

公开(公告)号：WO2021084116A2

公开(公告)日：2021-05-06

申请号：PCT/EP2020/080606

申请日：2020-10-30

Applicant: MURSLA LIMITED

Inventor： DIAS, Tomas Miguel De Freitas ,  ARSÈNE, Pierre

IPC: G01N33/543 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L3/502715 ,  B01L3/502761 ,  C12Q1/003 ,  G01N27/30 ,  G01N27/3274 ,  G01N27/3276 ,  G01N27/3278 ,  G01N33/54346 ,  G01N33/5438 ,  G01N33/54386 ,  G01N33/68

Abstract: A method of detecting a target biological entity in a biofluid using a sensor, wherein the biofluid comprises a plurality of the target biological entities and nanoparticles, the sensor comprising a substrate bearing a pair of electrodes having an affinity with the nanoparticles, and wherein a region between the electrodes defines a sensing region. The method comprises: treating the biofluid with a suspension comprising a plurality of nanoparticles to obtain a treated mixture comprising bound nanoparticle-entity assemblies; introducing the treated mixture to the sensor; conditioning the sensor in the presence of the treated mixture by applying an activation voltage between the electrodes to increase a degree of connection between a surface of the pair of electrodes and at least one bound nanoparticle-entity assembly in contact with the surface of the pair of electrodes; and detecting the presence of target biological entities by using the pair of electrodes to detect a current through the at least one bound nanoparticle-entity assembly.

公开(公告)号：WO2022140174A2

公开(公告)日：2022-06-30

申请号：PCT/US2021/064009

申请日：2021-12-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BEACHAM, Daniel W. ,  HINZ, Wolfgang ,  PARKER, Scott T. ,  DONOHUE, John

IPC: G01N27/414 ,  B01L3/00 ,  G01N33/487 ,  G01N35/00 ,  B01L2200/0668 ,  B01L2200/147 ,  B01L2300/027 ,  B01L2300/0663 ,  B01L2300/0829 ,  B01L2300/1827 ,  B01L2300/185 ,  B01L2300/1894 ,  B01L3/502715 ,  B01L7/00 ,  C12M1/00 ,  G01N15/00 ,  G02B21/00

Abstract: Systems, devices and methods for cell analysis provide an end user with real-time cell analysis and imaging of single cells in a population. Various cell analysis systems can provide both optical imaging, as well as electroscopic imaging, which is an image of cellular response as detected by sensors covering a cell footprint or cellular efflux. An automated fluidic system can provide an end-user selected sequence of reagents to cells, while precision controlled sensor array device thermostatting, and analysis compartment environmental control provide consistency in the cell analysis system environment.

公开(公告)号：WO2021259960A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067049

申请日：2021-06-22

Applicant: GNA BIOSOLUTIONS GMBH

Inventor： STEHR, Joachim ,  BUERSGENS, Federico ,  REBUFFO-SCHEER, Cecilia ,  GROTZ, Bettina ,  DUCZEK, Juliane ,  SCHMIDBAUER, Simon

IPC: C12Q1/6806 ,  B01L7/00 ,  C12N15/10 ,  B01L2200/0668 ,  B01L2300/0851 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L7/52 ,  C12N15/1013

Abstract: Bereitgestellt wird ein Verfahren zur Extraktion einer Target-Nukleinsäure (12) aus einer Probenflüssigkeit (30). Das Verfahren umfasst ein Bereitstellen einer Probenflüssigkeit (30) mit der Target-Nukleinsäure (12) in einem Reaktionsgefäß (22) und ein Bereitstellen von magnetischen Mikropartikeln (10) in der Probenflüssigkeit (30), die jeweils mit zumindest einer Extraktionsnukleinsäure (16) funktionalisiert sind, wobei die Extraktionsnukleinsäuren (16) zumindest teilweise komplementär zur Target-Nukleinsäure (12) sind. Ferner umfasst das Verfahren ein Hybridisieren zumindest eines Teils der Target-Nukleinsäure (12) mit einer der Extraktionsnukleinsäuren (16) und Binden der Target-Nukleinsäure (12) über die Extraktionsnukleinsäure (16) an eines der magnetischen Mikropartikeln (10). Außerdem umfasst das Verfahren ein Bereitstellen eines Magnetfeldes in dem Reaktionsgefäß (22) derart, dass zumindest ein Teil der mit der Target-Nukleinsäure (12) verbundenen magnetischen Mikropartikeln (10) an einer Gefäßwand (22a, 22b, 22c) des Reaktionsgefäßes (22) sedimentiert. Ferner werden Vorrichtungen und Verfahren zur Amplifikation einer Target-Nukleinsäure (12), die Verwendung von magnetischen Mikropartikeln (10) zur Extraktion einer Target-Nukleinsäure (12) und ein magnetisches Mikropartikel bereitgestellt.

公开(公告)号：WO2021245635A1

公开(公告)日：2021-12-09

申请号：PCT/IB2021/054981

申请日：2021-06-07

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

Inventor： THIRIET, Pierre-Emmanuel ,  GUIDUCCI, Carlotta ,  PORRO, Gloria

IPC: B01L3/00 ,  B03C5/00 ,  G01N1/00 ,  G01N27/00 ,  B03C5/02 ,  G01N1/40 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0848 ,  B01L2400/0424 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B03C2201/24 ,  B03C2201/26 ,  B03C5/005 ,  B03C5/026 ,  G01N2001/4038

Abstract: A dielectrophoretic detection device comprising a chip, with a flow channel having at least one inlet and one outlet, and at least a detection area configured to detect analytes trapped on functionalised beads flowing within said flow channel, a first and a second electrode assembly shaped as rows of parallel pillars extending over said height of the flow channel, configured to generate under an appropriate electric tension therebetween an electric field so as to form an electrical barrier, preventing said beads to cross said barrier and drawing said beads to said detection area by dielectrophoretic forces, where they are clustered and concentrated. The device may be provided with multiple rows of parallel pillars of electrode assemblies extending over the height of the flow channel, forming multiple concentration lines. The flow channel may be further provided with further rows of parallel pillars of electrode assemblies crossing said flow channel in transverse direction, forming further incubation lines.

公开(公告)号：WO2021222667A1

公开(公告)日：2021-11-04

申请号：PCT/US2021/030020

申请日：2021-04-29

Applicant: ABBOTT LABORATORIES ,  ARAI, Yoshiyuki ,  KOMORI, Tomotaka

Inventor： YOSHIMURA, Toru

IPC: B01L3/00 ,  G01N35/00 ,  B01L2200/0621 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0896 ,  B01L2400/043 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761

Abstract: Sample analysis systems and methods using assay surfaces, assay processing units (APUs), assay processing systems (APSs), and laboratory systems are disclosed. An assay surface includes a sample processing component comprising a plurality of regions, including at least one wash region and at least one storage region configured to hold a plurality of solid supports moveable through the regions under a magnetic force, and a detection component configured to receive the solid supports. An APU includes an assay surface receiving component, a magnetic element configured to generate a moveable magnetic field, and one or more processors configured to move the magnetic field. An APS includes one or more assay surfaces and an APU. A laboratory system includes one or more APSs and a controller for parallel processing. Sample processing and detection methods are disclosed with a reduced sample volume and/or shortened processing time and/or higher sensitivity.

公开(公告)号：WO2021191767A1

公开(公告)日：2021-09-30

申请号：PCT/IB2021/052355

申请日：2021-03-22

Applicant: CRESTOPTICS S.P.A. ,  FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

Inventor： FOLLI, Viola ,  CAPRINI, Davide ,  CECCARELLI, Raino

IPC: C12Q1/02 ,  B01L3/00 ,  C12M1/34 ,  C12M3/00 ,  B01L2200/0668 ,  B01L2300/0877 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  C12M23/16 ,  C12Q1/025

Abstract: The present invention relates to a microfluidic device (100) for a system of detecting the neuronal activity of a population of sample micro-organisms, which device (10) comprises: - a conduit (10) comprising an intermediate portion (13) arranged between an inlet portion (11) and an outlet portion (12) of an operating fluid in/from the conduit (10), said conduit (10) being shaped so as to receive the operating fluid crossing through a longitudinal direction (A), - a stagnation chamber (20) configured to receive internally the population of sample micro-organisms, wherein said stagnation chamber (20) is in fluid communication with said conduit (10) through one or more openings (30) obtained in the intermediate portion (13) and configured to keep unchanged, in use, the crossing direction of the operating fluid, and wherein the ratio between the cross section passage (o) of the operating fluid in the conduit (10) at said intermediate portion (13) and the cross section passage of the operating fluid in the conduit (10) at said inlet portion (11) or at said outlet portion (12) is greater than 1.