公开(公告)号：WO2023057642A1

公开(公告)日：2023-04-13

申请号：PCT/EP2022/077989

申请日：2022-10-07

申请人： HOMEDICUS GMBH

发明人： RIESTER, Markus ,  DIEBOLD, Michael

IPC分类号： A61B5/15 ,  A61B5/151 ,  A61B5/157 ,  B01L3/00 ,  F16K99/00 ,  A61B5/150022 ,  A61B5/150221 ,  A61B5/150251 ,  A61B5/150358 ,  A61B5/150389 ,  A61B5/150419 ,  A61B5/15107 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0803 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0688 ,  B01L3/502738 ,  F16K99/0021

摘要： The invention relates to a liquid sampling device (100) comprising a support structure (106) having a liquid reception interface (130) that is in fluid communication with a reservoir chamber (134) and a microfluidic valve (136), the microfluidic valve (136) being connected to a measuring chamber (138). The liquid reception interface (130), the reservoir chamber (134) and the microfluidic valve (136) are configured to allow liquid entering the liquid reception interface to flow into the reservoir chamber (134) while being prevented from passing the microfluidic valve (136) unless a predetermined static pressure difference over the microfluidic valve (136) is exceeded. The microfluidic valve (136) is a passive valve without moving parts. Passing of liquid through the microfluidic valve is controlled by a static pressure difference overthe microfluidic valve (136). The reservoir chamber (134) is configured and arranged to cause high enough a static pressure at an entrance side of the microfluidic valve (136) that causes a flow of liquid through the microfluidic valve (136) into the measuring chamber (138) once the reservoir chamber (134) is completely filled.

公开(公告)号：WO2023275831A1

公开(公告)日：2023-01-05

申请号：PCT/IB2022/056132

申请日：2022-06-30

申请人： CREGANNA UNLIMITED COMPANY

发明人： WRIGHT, David W. ,  MITCHELL, Fred ,  CASTILLO, Benjamin A.

IPC分类号： B01L3/00 ,  C12Q1/00 ,  G01N27/327 ,  B01F33/30 ,  G01N35/00 ,  B01F33/304 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/049 ,  B01L2400/0683 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/523 ,  C12Q1/006

摘要： A disposable diagnostic device (10) includes a body (12) having a first channel (14) and a second channel (16) spaced from the first channel. A blister (18) is operably fixed to the body and encloses a chamber (20) which is configured in a hermetically sealed-off relation from the first and second channels when the device is in a non-activated first state and is in open communication with at least one of the first and second channels when the device is in an activated second state. A reactant (22) and an inert gas (24) are disposed in the chamber such that the inert gas protects the reactant from being exposed to contaminants when the device is in said non-activated first state. A method of constructing a disposable diagnostic device is also disclosed.

公开(公告)号：WO2023059183A1

公开(公告)日：2023-04-13

申请号：PCT/NL2022/050551

申请日：2022-10-03

申请人： MICRONIT HOLDING B.V.

发明人： BLOM, Marko Theodoor ,  VAN 'T OEVER, Ronny ,  CONDE, Alvaro Jose

IPC分类号： B01L3/00 ,  B01L2200/0621 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2300/042 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L3/502738 ,  B01L3/502746

摘要： Microfluidic device comprising a microfluidic circuit comprising a main fluid channel and an inlet coupled to the main fluid channel for introducing fluid into the microfluidic circuit and at least one fluid chamber configuration, wherein the fluid chamber configuration comprises: - at least one fluid chamber; - a fluid chamber inlet channel coupling the main fluid channel to an inlet of the fluid chamber; - a fluid chamber outlet channel coupled to an outlet of the fluid chamber; - a pressure stop valve arranged in the main fluid channel downstream of the coupling with the fluid chamber inlet channel, wherein the pressure stop valve is arranged to block fluid at a pressure below a first burst pressure; - a flow restrictor arranged in the fluid chamber outlet channel, wherein the flow restrictor is arranged to provide a back pressure which is higher than the first burst pressure of the pressure stop valve.

公开(公告)号：WO2021252671A2

公开(公告)日：2021-12-16

申请号：PCT/US2021/036670

申请日：2021-06-09

申请人： ELEMENT BIOSCIENCES, INC.

发明人： GUO, Minghao ,  ZHANG, Leon Zilun ,  ZHOU, Chunhong ,  KELLINGER, Matthew ,  PREVITE, Michael ,  ARSLAN, Sinan ,  HE, Molly ,  MAH, Huizhen ,  SUN, Lei

IPC分类号： C12Q1/6837 ,  C12Q1/6869 ,  C12M1/34 ,  C12N15/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L3/502715 ,  B01L3/502738 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/701 ,  G01N2021/6439 ,  G01N21/6428

摘要： Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：WO2021250060A1

公开(公告)日：2021-12-16

申请号：PCT/EP2021/065393

申请日：2021-06-09

申请人： MILTENYI BIOTEC B.V. & CO. KG

发明人： ROCKENBACH, Alexander ,  MILTENYI, Stefan

IPC分类号： B01L3/00 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/088 ,  B01L2400/043 ,  B01L2400/0633 ,  B01L3/502738 ,  B01L3/502761 ,  B01L3/502784

摘要： A microfabricated droplet dispensing structure is described, which may include a plurality of MEMS microfluidic fluidic valves, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target particle and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. At least one additional microfabricated valve may sort a barcoded bead, wherein the sorted bead is conjugated with the sorted cell to make a bead/cell complex. The complex may be enclosed in an aqueous droplet and the droplet dispensed into a stream of a second immiscible fluid.

公开(公告)号：WO2020257356A2

公开(公告)日：2020-12-24

申请号：PCT/US2020/038242

申请日：2020-06-17

申请人： MAMMOTH BIOSCIENCES, INC. ,  BROUGHTON, James Paul ,  SINGH, Jasmeet ,  FASCHING, Clare Louise ,  TSALOGLOU, Maria-Nefeli ,  GALARZO, Pedro Patrick Draper ,  CHEN, Janice Sha ,  MIAO, Xin ,  HARRINGTON, Lucas ,  DRZAL, Daniel Thomas ,  SHAPIRO, Sarah Jane

发明人： BROUGHTON, James Paul ,  SINGH, Jasmeet ,  FASCHING, Clare Louise ,  TSALOGLOU, Maria-Nefeli ,  GALARZO, Pedro Patrick Draper ,  CHEN, Janice Sha ,  MIAO, Xin ,  HARRINGTON, Lucas ,  DRZAL, Daniel Thomas ,  SHAPIRO, Sarah Jane

IPC分类号： B01L3/00 ,  B01L7/00 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0666 ,  B01L3/502738 ,  B01L7/525

摘要： Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids.

公开(公告)号：WO2023274997A1

公开(公告)日：2023-01-05

申请号：PCT/EP2022/067647

申请日：2022-06-28

申请人： ETH ZURICH

发明人： CAO, Xiaobao ,  XUE, Peng ,  AEBERSOLD, Rudolf ,  STAVRAKIS, Stavros ,  DEMELLO, Andrew James

IPC分类号： B01L3/00 ,  G01N33/68 ,  G01N1/40 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/0655 ,  B01L2400/086 ,  B01L3/502738 ,  G01N33/6842

摘要： In a method for analyzing cells, a sample fluid comprising a suspending medium and cells is fed to a microfluidic device comprising at least one cell processing unit (10) comprising a trapping region (12), a reaction unit (20), and an outlet arrangement (30). The trapping region is delimited by at least an input valve (13) and a sieve valve (14), in particular a v- type valve that is capable of retaining the cells while letting fluids pass. The method comprises trapping cells in the trapping region, subsequently establishing a flow of a reaction fluid through the trapping region while the sieve valve (14) assumes said open state, such that the reaction fluid transfers the trapped cells from the trapping region (12) into the reaction unit (20), decomposing the transferred cells into cell fragments through a decomposition process, collecting the cell fragments in the outlet arrangement, and analyzing the cell fragments.

公开(公告)号：WO2022060536A1

公开(公告)日：2022-03-24

申请号：PCT/US2021/047081

申请日：2021-08-23

申请人： INTELLIGENT VIRUS IMAGING INC

发明人： HAUSER, Janosch ,  KYLBERG, Gustaf ,  STEMME, Göran ,  SINTORN, Ida-Maria ,  ROXHED, Niclas

IPC分类号： B01L3/502738 ,  G01N1/30 ,  G01N1/31 ,  H01J37/16 ,  H01J37/20 ,  H01J37/252

摘要： The microfluidic device (100, 200) has a first reservoir (102, 202) that preferably includes a first liquid (108, 208). The first liquid (108, 208) is being held by a capillary stop valve (228, 230) in the first reservoir (102, 202). A second reservoir (104, 204) is in fluid communication with the first reservoir (102, 202). The second reservoir (104, 204) has a second liquid (110, 210) and a sample support (116, 216) disposed therein. The second reservoir (104, 204) has an inlet opening (145, 236) defined therein. A draining unit (106, 206) is adjacent to the second reservoir (104, 204). The draining unit (106, 206) is in fluid communication with the second reservoir (104, 204). The draining unit (106, 206) has a first absorption member (158, 218, 858) disposed therein.

公开(公告)号：WO2021198297A1

公开(公告)日：2021-10-07

申请号：PCT/EP2021/058364

申请日：2021-03-30

申请人： OSLER DIAGNOSTICS LIMITED

发明人： LILLIS, Barry ,  GREEN, Steven ,  HOLLAND, Damian

IPC分类号： B01L3/00 ,  F16K11/16 ,  F16K31/524 ,  F16K99/00 ,  B01L2200/025 ,  B01L2300/0887 ,  B01L2400/0655 ,  B01L3/502715 ,  B01L3/502738 ,  F16K11/165 ,  F16K31/52491 ,  F16K99/0026 ,  F16K99/0028 ,  F16K99/0042

摘要： The present disclosure relates to an actuator, an actuator assembly, a method of operating an actuator, a computer program and a system. In one aspect, an actuator may comprise a plurality of independently operable actuation elements and an operator having an operator surface. Each actuation element may have an actuation surface. The operator may be driveable to move the operator surface along a path to selectively engage with the respective actuation surface of each actuation element to actuate the actuation element.

公开(公告)号：WO2021086945A

公开(公告)日：2021-05-06

申请号：PCT/US2020/057708

申请日：2020-10-28

申请人： QUANTUM-SI INCORPORATED [US]/[US]

发明人： ROTHBERG, Jonathan, M. ,  LEAMON, John, H. ,  SCHULTZ, Jonathan, C. ,  MILLHAM, Michele ,  LV, Caixia ,  MA, Xiaxiao

IPC分类号： C12Q1/6806 ,  B03C7/02 ,  C12N15/10 ,  G01N33/68 ,  A61B5/15 ,  B01L3/00 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0631 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0887 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/52 ,  C12N15/1003 ,  C12N15/101 ,  G01N33/6818

摘要： Methods and devices for isolating or enriching target molecules from a sample using lysis, fragmentation and affinity purification, e.g., using scodaphoresis, are provided herein. In particular embodiments, a device for enriching a target molecule from a biological sample is characterized in that the device comprises an automated sample preparation module comprising a cartridge housing that is configured to receive a removable cartridge. In some embodiments, methods and devices further involve detection, analysis and/or sequencing of a target molecule.