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52 条记录 (耗时 0.024 秒)

    MICROARRAY FOR DETECTING AND QUANTIFYING MICRORNA
    24.
    发明申请
    MICROARRAY FOR DETECTING AND QUANTIFYING MICRORNA 审中-公开

    公开(公告)号：WO2008048342A3

    公开(公告)日：2008-04-24

    申请号：PCT/US2007/003116

    申请日：2007-02-07

    申请人： DHARMACON, INC. ,  BASKERVILLE, Scott ,  JUCKER, Fiona, M. ,  KHVOROVA, Anastasia

    发明人： BASKERVILLE, Scott ,  JUCKER, Fiona, M. ,  KHVOROVA, Anastasia

    IPC分类号： C12Q1/68 ,  C12M1/34

    摘要： A microarray can be configured for hybridizing with short nucleic acids, such as miRNA or siRNA, contained within a sample. Such a microarray includes a polynucleotide trap coupled to a substrate of a microarray site. The polynucleotide trap includes a probe that selectively hybridizes with short nucleic acid sequences, and a linker that is coupled to the substrate and configured to extend the probe from the substrate. Additionally, the polynucleotide trap can include an enhancer that hybridizes with the linker in order to enhance functionality of the probe. The linker and/or enhancer are configured to inhibit the probe from interacting with the linker or substrate. This can include the linker and/or enhancer being configured to have a minimal secondary structure so as to present the probe region for hybridizing with the target polynucleotide and inhibit the linker region or probe region from interacting with the substrate.

    MICROARRAY FOR DETECTING AND QUANTIFYING MICRORNA
    25.
    发明申请
    MICROARRAY FOR DETECTING AND QUANTIFYING MICRORNA 审中-公开
    用于检测和定量MICRORNA的微阵列

    公开(公告)号：WO2008048342A2

    公开(公告)日：2008-04-24

    申请号：PCT/US2007003116

    申请日：2007-02-07

    申请人： DHARMACON INC ,  BASKERVILLE SCOTT ,  JUCKER FIONA M ,  KHVOROVA ANASTASIA

    发明人： BASKERVILLE SCOTT ,  JUCKER FIONA M ,  KHVOROVA ANASTASIA

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q2525/207 ,  C12Q2525/197 ,  C12Q2525/161 ,  C12Q2537/163 ,  C12Q2565/525

    摘要： A microarray can be configured for hybridizing with short nucleic acids, such as miRNA or siRNA, contained within a sample. Such a microarray includes a polynucleotide trap coupled to a substrate of a microarray site. The polynucleotide trap includes a probe that selectively hybridizes with short nucleic acid sequences, and a linker that is coupled to the substrate and configured to extend the probe from the substrate. Additionally, the polynucleotide trap can include an enhancer that hybridizes with the linker in order to enhance functionality of the probe. The linker and/or enhancer are configured to inhibit the probe from interacting with the linker or substrate. This can include the linker and/or enhancer being configured to have a minimal secondary structure so as to present the probe region for hybridizing with the target polynucleotide and inhibit the linker region or probe region from interacting with the substrate.

    摘要翻译： 微阵列可以配置用于与样品中包含的短核酸（例如miRNA或siRNA）杂交。 这样的微阵列包括与微阵列位点的基底偶联的多核苷酸捕获物。 多核苷酸捕获物包括与短核酸序列选择性杂交的探针以及偶联于基底并被配置为从基底延伸探针的接头。 此外，多核苷酸诱捕器可以包括与接头杂交以增强探针功能的增强子。 接头和/或增强子被配置为抑制探针与接头或基底相互作用。 这可以包括接头和/或增强子被配置为具有最小的二级结构，以便呈递探针区域用于与靶标多核苷酸杂交并且抑制接头区域或探针区域与底物相互作用。

    APPARATUS AND SYSTEM HAVING DRY GENE SILENCING COMPOSITIONS
    27.
    发明申请
    APPARATUS AND SYSTEM HAVING DRY GENE SILENCING COMPOSITIONS 审中-公开
    具有干基因沉默组合物的装置和系统

    公开(公告)号：WO2006071410A3

    公开(公告)日：2007-05-03

    申请号：PCT/US2005042404

    申请日：2005-11-21

    申请人： DHARMACON INC ,  ROBERTSON BARBARA ,  LEAKE DEVIN ,  ROBINSON KATHRYN ,  MARSHALL WILLIAM S ,  KHVOROVA ANASTASIA

    发明人： ROBERTSON BARBARA ,  LEAKE DEVIN ,  ROBINSON KATHRYN ,  MARSHALL WILLIAM S ,  KHVOROVA ANASTASIA

    IPC分类号： C12N15/63 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/68

    CPC分类号： C12N15/111 ,  C12N2310/14 ,  C12N2320/12 ,  C12N2330/31

    摘要： A reverse transfection apparatus can be used for introducing siRNA into a cell to effect gene silencing. Such an apparatus can include a well plate having a well configured for transfecting cells. The well can include a substantially dry gene silencing composition that has at least a first siRNA which silences a first target gene. The gene silencing composition can be configured such that the at least first siRNA is capable of being solubilized or suspended in an aqueous medium in an amount sufficient for transfecting cells in the well. Additionally, the at least first siRNA can include a modification or a conjugate. The reverse transfection apparatus can be provided as a kit or system that additionally includes cells, polynucleotide carriers, reverse transfection reagents, and the like.

    摘要翻译： 反向转染装置可用于将siRNA引入细胞以进行基因沉默。 这样的装置可以包括具有良好配置用于转染细胞的孔板。 该孔可以包括至少具有使第一靶基因沉默的第一siRNA的基本上干的基因沉默组合物。 基因沉默组合物可以配置成使得至少第一siRNA能够以足以转染孔中的细胞的量溶解或悬浮在水性介质中。 另外，至少第一siRNA可以包括修饰或缀合物。 反向转染装置可以作为另外包含细胞，多核苷酸载体，反向转染试剂等的试剂盒或系统来提供。

    APPARATUS AND SYSTEM HAVING DRY CONTROL GENE SILENCING COMPOSITIONS
    28.
    发明申请
    APPARATUS AND SYSTEM HAVING DRY CONTROL GENE SILENCING COMPOSITIONS 审中-公开
    具有干燥控制基因沉默组合物的装置和系统

    公开(公告)号：WO2006060246A2

    公开(公告)日：2006-06-08

    申请号：PCT/US2005/042385

    申请日：2005-11-21

    申请人： DHARMACON, INC. ,  ROBERTSON, Barbara ,  LEAKE, Devin ,  ROBINSON, Kathryn ,  MARSHALL, William, S. ,  KHVOROVA, Anastasia

    发明人： ROBERTSON, Barbara ,  LEAKE, Devin ,  ROBINSON, Kathryn ,  MARSHALL, William, S. ,  KHVOROVA, Anastasia

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12N15/87 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/11 ,  C12N2330/30

    摘要： An RTF testing plate can include at least a first control well including a substantially dry first control composition having at least a first control siRNA. The first control siRNA is capable of providing a first indication of the gene silencing efficacy. Additionally, the first control composition can be configured such that the first control siRNA is capable of being solubilized or suspended in an aqueous medium in an amount sufficient for transfecting cells in the first control well. The control siRNA can be any one of a transfection control siRNA, positive control siRNA, or negative control siRNA. Optionally, the total amount of control siRNA in the first control composition can be present in an amount for transfecting cells in only the first control well.

    摘要翻译： RTF测试板可以包括至少第一对照孔，其包含具有至少第一对照siRNA的基本上干的第一对照组合物。 第一对照siRNA能够提供基因沉默功效的第一指示。 另外，可以配置第一对照组合物，使得第一对照siRNA能够以足以转染第一对照孔中的细胞的量溶解或悬浮在水性介质中。 对照siRNA可以是转染对照siRNA，阳性对照siRNA或阴性对照siRNA中的任一种。 任选地，第一对照组合物中对照siRNA的总量可以以仅在第一对照孔中转染细胞的量存在。