公开(公告)号：WO99033862A1

公开(公告)日：1999-07-08

申请号：PCT/US1998/026251

申请日：1998-12-10

IPC: C12N15/09 ,  B01D11/04 ,  B01D15/02 ,  B01D15/08 ,  B01D61/02 ,  B01D61/14 ,  C07K1/16 ,  C07K1/20 ,  C07K1/22 ,  C07K1/32 ,  C12N7/02 ,  G01N30/02 ,  G01N30/06 ,  G01N30/88

CPC classification number: C07K1/34 ,  C07K1/16 ,  C07K1/20 ,  C07K1/22 ,  C07K1/32 ,  C07K1/36 ,  G01N30/02 ,  G01N30/06 ,  G01N2030/8813 ,  G01N2030/8827 ,  G01N2030/8831 ,  Y10S436/828 ,  B01D15/34

Abstract: The present invention is directed to novel methods for enhancing the ability to separate a species of interest from other different species present in a free solution mixture thereof which takes advantage of interactions that occur between soluble, small molecular weight ligands and the species of interest. The small molecular weight ligands employed in the present invention function to interact with a species of interest in a mixture of different species through either affinity, hydrophobic and/or ionic interactions, thereby altering the molecular weight, hydrodynamic volume and/or isoelectric point of the species of interest and rendering it separable from other component(s) in the mixture.

Abstract translation: 本发明涉及增强从存在于其游离溶液混合物中的其它不同物种分离感兴趣物种的能力的新方法，其利用可溶性，小分子量配体和感兴趣物种之间发生的相互作用。 本发明中使用的小分子量配体通过亲和力，疏水和/或离子相互作用在不同物种的混合物中与感兴趣的物种相互作用，由此改变了分子量，流体动力学体积和/或等电点 感兴趣的物种，使其与混合物中的其他成分分离。

公开(公告)号：WO9846623A3

公开(公告)日：1999-01-28

申请号：PCT/GB9801109

申请日：1998-04-16

Applicant: MARSDEN JOHN CHRISTOPHER ,  AGNER ERIK

Inventor： AGNER ERIK

IPC: B01D15/08 ,  B01D15/36 ,  B01D15/42 ,  C07K1/16 ,  G01N30/02 ,  G01N30/46

CPC classification number: G01N30/468 ,  B01D15/08 ,  B01D15/363 ,  B01D15/422 ,  C07K1/16 ,  G01N30/02 ,  G01N30/461 ,  G01N30/466 ,  B01D15/36

Abstract: Sample displacement chromatography is performed at low operating pressures (less than 30 bar) and/or at high sample loading (in excess of 500 mg/cm2 column area), with product recovery being effected in a non-gradient manner. The low operating pressures permit the use of simple and inexpensive apparatus. Non-gradient product recovery allows the desired product to be allowed in solutions with advantageously high concentration. Materials which may be purified include pharmaceuticals, pharmaceutical excipients, fine chemicals, biochemicals, X-ray contrast agents, chelating agents, peptides, proteins, oligonucleotides and vaccines. Apparatus embodiments include sample displacement chromatography apparatus comprising one or more ion exchange columns in direct combination with one or more desalting columns, and multicolumn chromatography apparatus permitting switching between a series separation mode and a parallel extraction mode.

公开(公告)号：WO98046623A2

公开(公告)日：1998-10-22

申请号：PCT/GB1998/001109

申请日：1998-04-16

IPC: B01D15/08 ,  B01D15/36 ,  B01D15/42 ,  C07K1/16 ,  G01N30/02 ,  G01N30/46

CPC classification number: G01N30/468 ,  B01D15/08 ,  B01D15/363 ,  B01D15/422 ,  C07K1/16 ,  G01N30/02 ,  G01N30/461 ,  G01N30/466 ,  B01D15/36

Abstract: Sample displacement chromatography is performed at low operating pressures (less than 30 bar) and/or at high sample loading (in excess of 500 mg/cm column area), with product recovery being effected in a non-gradient manner. The low operating pressures permit the use of simple and inexpensive apparatus. Non-gradient product recovery allows the desired product to be allowed in solutions with advantageously high concentration. Materials which may be purified include pharmaceuticals, pharmaceutical excipients, fine chemicals, biochemicals, X-ray contrast agents, chelating agents, peptides, proteins, oligonucleotides and vaccines. Apparatus embodiments include sample displacement chromatography apparatus comprising one or more ion exchange columns in direct combination with one or more desalting columns, and multicolumn chromatography apparatus permitting switching between a series separation mode and a parallel extraction mode.

Abstract translation: 在低操作压力（小于30巴）和/或高样品负载（超过500mg / cm 2柱面积）下进行样品置换色谱，产物回收以非梯度方式进行。 低操作压力允许使用简单且便宜的装置。 非梯度产物回收允许所需产物在有利地高浓度的溶液中被允许。 可以纯化的材料包括药物，药物赋形剂，精细化学品，生物化学品，X射线造影剂，螯合剂，肽，蛋白质，寡核苷酸和疫苗。 装置实施方案包括样品置换色谱装置，其包括与一个或多个脱盐塔直接组合的一个或多个离子交换柱，以及允许在串联分离模式和并行提取模式之间切换的多色谱色谱装置。

公开(公告)号：WO1991015502A1

公开(公告)日：1991-10-17

申请号：PCT/JP1991000421

申请日：1991-03-29

Applicant: SHISEIDO COMPANY, LTD. ,  KAMINUMA, Toshihiko ,  IIDA, Toshii ,  TAJIMA, Masahiro

Inventor： SHISEIDO COMPANY, LTD.

IPC: C07K01/14

CPC classification number: C07K1/16 ,  C07K1/36

Abstract: An improved process for purifying a polypeptide with a packing for high-performance liquid chromatography, which comprises preteating polypeptides produced by various cells into a given state, adjusting the pH of an aqueous solution of the polypeptides to fall within a specified range to thereby remove contaminants by precipitation, and treating the remaining solution with a packing for reversed-phase high-performance liquid chromatography.

公开(公告)号：WO1991007427A1

公开(公告)日：1991-05-30

申请号：PCT/JP1990001493

申请日：1990-11-15

Applicant: NISSEI CHEMICAL INDUSTRY CO., LTD. ,  MAKINO, Kenjiro ,  YADA, Tomoaki ,  SAKOU, Masayuki ,  HATANAKA, Chitoshi ,  MURAO, Sawao

Inventor： NISSEI CHEMICAL INDUSTRY CO., LTD.

IPC: C07K03/20

CPC classification number: C07K1/16 ,  B01J20/22

Abstract: A carrier for column chromatography and its applications. The carrier is characterized by comprising a pectic acid-cellulose gel. When it is used for ion exchange chromatography and gel filtration chromatography, a water-soluble polymeric substance, particularly protein, is stably separated and purified, so that the recovery can be improved. Further, the removal of structural protein and cell wall-binding protein in the pectic acid-cellulose gel serves to reduce a non specific adsorption by remaining proteins.

Abstract translation: 柱色谱的载体及其应用。 载体的特征在于包含果胶酸纤维素凝胶。 当用于离子交换层析和凝胶过滤色谱法时，水溶性聚合物质，特别是蛋白质，被稳定地分离和纯化，从而可以提高回收率。 此外，在果胶 - 纤维素凝胶中去除结构蛋白和细胞壁结合蛋白用于减少残留蛋白质的非特异性吸附。

公开(公告)号：WO2019095592A1

公开(公告)日：2019-05-23

申请号：PCT/CN2018/079258

申请日：2018-03-16

Applicant: 深圳翰宇药业股份有限公司

Inventor： 陈友金 ,  宓鹏程 ,  陶安进 ,  袁建成

IPC: C07K1/02 ,  C07K1/08 ,  C07K1/16 ,  C07K7/06

CPC classification number: C07K1/02 ,  C07K1/06 ,  C07K1/08 ,  C07K1/16 ,  C07K7/06

Abstract: 提供一种Reltecimod的液相制备方法，包括如下步骤：步骤1)，合成三个全保护的多肽片段，R1-Ser(R 4 )-Pro-OH，R 2 -Met-Leu-Val-OH和R 3 -Ala-Tyr(R 5 )-Asp(R 6 )-OH；步骤2)偶联R 2 -Met-Leu-Val-OH和R 3 -Ala-Tyr(R 5 )-Asp(R 6 )-OH，得到R2-Met-Leu-Val-Ala-Tyr(R 5 )-Asp(R6)-OH；步骤3)偶联R1-Ser(R 4 )-Pro和R2-Met-Leu-Val-Ala-Tyr(R 5 )-Asp(R 6 )-OH，得到全保护多肽；步骤4)脱除保护基，得到粗品；步骤5)纯化。

公开(公告)号：WO2019032946A1

公开(公告)日：2019-02-14

申请号：PCT/US2018/046188

申请日：2018-08-10

Applicant: FERRING B.V. ,  PETERSON, Raymond ,  HAMILTON, Robert ,  CHEN, Lin ,  OINEN, Mark

Inventor： PETERSON, Raymond ,  HAMILTON, Robert ,  CHEN, Lin ,  OINEN, Mark

IPC: A61P15/04 ,  A61K38/11 ,  C07K7/16 ,  A61P15/02

CPC classification number: C07K1/16 ,  A61K38/095 ,  A61P5/10 ,  A61P7/04 ,  A61P15/02 ,  A61P15/04 ,  B01D9/0054 ,  B01D15/163 ,  C07K1/306 ,  C07K7/06 ,  C07K7/16

Abstract: A method of manufacturing oxytocin or oxytocin receptor agonist comprising a step of combining an antisolvent with a solution comprising oxytocin or oxytocin receptor agonist so as to precipitate a product oxytocin or a product oxytocin receptor agonist from the solution.

公开(公告)号：WO2018045587A1

公开(公告)日：2018-03-15

申请号：PCT/CN2016/098714

申请日：2016-09-12

Applicant: 广东东阳光药业有限公司

Inventor： 杨辉 ,  马旭通 ,  杨彬 ,  林小鹊 ,  李文佳

IPC: C07K16/22 ,  C07K1/16

CPC classification number: C07K1/16 ,  C07K16/22

Abstract: 一种抗VEGF类单克隆抗体的纯化方法，包括首先用复合材料层析进行捕获，使抗体与收获液中的大多数组分分离，继而用羟基磷灰石层析进行精细纯化，进一步去除宿主细胞污染物和聚集体等，所述的复合层析材料为离子交换作用和疏水作用的复合介质。该方法同时降低宿主蛋白(HCP)、DNA、多聚体、酸性峰的含量，从而达到显著提高抗体纯度的目的，且操作简单，成本较低。

公开(公告)号：WO2017136785A1

公开(公告)日：2017-08-10

申请号：PCT/US2017/016595

申请日：2017-02-03

Applicant: PLASMA TECHNOLOGIES, LLC

Inventor： ZURLO, Eugene ,  NOWOTNIK, David Peter ,  HELDEBRANT, Charles ,  CURTIN, Dennis

IPC: C07K1/30 ,  C07K1/16 ,  C07K1/22 ,  C07K1/34 ,  C07K1/36 ,  C07K14/81 ,  C07K16/06

CPC classification number: C07K1/16 ,  A61K38/16 ,  C07K1/22 ,  C07K1/30 ,  C07K1/34 ,  C07K14/81 ,  C07K16/06

Abstract: Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of: salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-detergent treatment, and sterile filtration. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.

Abstract translation: 本文描述了由血基材料（包括α-1-蛋白酶抑制剂，γ球蛋白，白蛋白和其他蛋白质）生产多种蛋白质产物的方法。 本发明的方法包括以下步骤：盐分级分离，色谱法，超滤，渗滤，溶剂 - 去污剂处理和无菌过滤。 有利的是，本发明的方法简单并且以高产率产生α-1-蛋白酶抑制剂，γ-球蛋白，白蛋白和其他蛋白质。 可以选择工艺步骤的顺序以获得来自各种过程中材料的多种产物，例如上清液，糊剂，层析流过液和层析液洗涤液。

公开(公告)号：WO2016206597A1

公开(公告)日：2016-12-29

申请号：PCT/CN2016/086856

申请日：2016-06-23

Applicant: 韩震

Inventor： 韩震

IPC: C07K7/08 ,  C07K14/00 ,  C07K1/16 ,  C07K1/06 ,  C07K1/04 ,  A61K38/10 ,  A61K38/16 ,  A61P35/00 ,  A61P35/02 ,  A61P37/04 ,  A61P37/02 ,  A61P31/00

CPC classification number: C07K14/001 ,  A61K38/00 ,  A61K38/10 ,  A61K38/16 ,  A61P37/04 ,  C07K1/04 ,  C07K1/042 ,  C07K1/06 ,  C07K1/061 ,  C07K1/16 ,  C07K7/08 ,  C07K14/00

Abstract: 公开了一种多肽化合物及其制备方法与应用，该多肽化合物结构式为：(X A X B X C X D X E -X) 2 KY，或{(X A X B X C X D X E -X) 2 K} 2 KY，或{({X A X B X C X D X E -X} 2 K) 2 K} 2 KY；其中：X A 为极性氨基酸分子，X B 和X E 为碱性氨基酸分子(可相同也可不相同)，X C 和X D 为非极性氨基酸分子(可相同也可不相同)，K为赖氨酸(Lys,K)，X和Y为空或任意一个或多个氨基酸或化学基团。所述多肽化合物具有增强免疫的作用。