公开(公告)号：WO2021188350A1

公开(公告)日：2021-09-23

申请号：PCT/US2021/021857

申请日：2021-03-11

Applicant: CAPOLUPO, Wayne

Inventor： CAPOLUPO, Wayne

IPC: A41D19/015 ,  A41D19/00 ,  A47K10/42 ,  B65D83/08 ,  B65D85/18

Abstract: Devices, kits and methods are provided to reduce or eliminate fecal contamination and cross-contamination and transmission of fecal-born pathogenic organisms by users employed in the food production and food preparation industries or users in a home, for example in restaurants, cruise ships, schools, hospitals, dormitories, transportation means and terminals, and government buildings, the devices and methods pertaining to a glove-like garment that is applied prior to disposal of feces and removed after disposal of toilet tissue. The glove is flushable, and in an embodiment includes means of retraction from the wrist area for inversion of the garment, followed by flushing the inverted removed glove.

公开(公告)号：WO2018081382A1

公开(公告)日：2018-05-03

申请号：PCT/US2017/058470

申请日：2017-10-26

Applicant: BROWN UNIVERSITY ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  UNIVERSITY OF KANSAS ,  THE TRUSTEES OF DARTMOUTH COLLEGE

Inventor： KELSEY, Karl ,  WIENCKE, John ,  KOESTLER, Devin ,  CHRISTENSEN, Brock

IPC: C12Q1/6888 ,  C12Q1/6883 ,  C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12Q1/68

Abstract: Ratio of neutrophils to lymphocytes (NLR) is here associated with immune suppression and decreased survival times in multiple solid tumors. Based on immune cell-specific DMRs and validated cell deconvolution algorithms, the NLR in blood from glioma patients was estimated and glioma patients had elevated mdNLR scores compared to controls. The patient mdNLR scores were increased in patients with grade IV tumors compared to grade II/III. High mdNLR scores were associated with shorter survival. Candidate single (myeloid-associated) gene loci that were highly correlated with the mdNLR were identified. Single myeloid differentiation loci provide a simpler and cheaper alternative to the mdNLR, which requires complex array data. Immunomethylomics are useful and more convenient than conventional cell analysis in profiling glioma risk and survival.

Abstract translation: 这里中性粒细胞与淋巴细胞（NLR）的比率在多种实体瘤中与免疫抑制和存活时间减少相关。 基于免疫细胞特异性DMR和验证的细胞去卷积算法，估计胶质瘤患者血液中的NLR，并且神经胶质瘤患者与对照相比具有升高的mdNLR评分。 与II / III级相比，患有IV级肿瘤的患者mdNLR评分增加。 高mdNLR评分与生存期较短有关。 鉴定了与mdNLR高度相关的候选单个（骨髓相关）基因位点。 单一髓样分化基因座为mdNLR提供了一种更简单，更便宜的替代方法，这需要复杂的阵列数据。 在分析神经胶质瘤风险和生存期方面，免疫甲基组学比常规细胞分析更有用和更方便。

公开(公告)号：WO2017112931A1

公开(公告)日：2017-06-29

申请号：PCT/US2016/068517

申请日：2016-12-23

Applicant: RHODE ISLAND HOSPITAL ,  BROWN UNIVERSITY

Inventor： PARK, William Keun Chan ,  DUPUY, Damian E. ,  WALSH, Edward G.

IPC: A61B18/00 ,  A61B18/04 ,  A61B18/18 ,  A61N5/02 ,  A61F2/01

CPC classification number: A61B18/14 ,  A61B18/1815 ,  A61B2018/00095 ,  A61B2018/00577 ,  A61B2018/00982

Abstract: A thermal accelerant is delivered to a tissue site and localized to modulate the shape, extent or other characteristic of RF or microwave-induced hyperthermic tissue ablation. The accelerant may be provided via an image-guided hand piece or via a lumen added to a microwave antenna, and promotes faster heating, more complete ablation and/or a more extensive treatment region to reduce recurrence of treated cancers, overcoming natural limitations, variations in tissue response and drop-off or thermal loss away from the antenna. The accelerant is delivered as a low-viscosity but heat sensitive fluid, and is fixed in place to provide regions of preferential absorption or heating. Shorter exposure times to heat the far field may allow survival of vulnerable tissue such as vessels, and multiple antennae may be used for effective treatment of irregular or large tumors.

Abstract translation: 将热促进剂递送至组织部位并定位以调节RF或微波诱导的高热组织消融的形状，程度或其他特征。 促进剂可以通过图像引导的手持件或经由添加到微波天线的内腔来提供，并且促进更快的加热，更彻底的消融和/或更广泛的治疗区域，以减少治疗的癌症的复发，克服自然限制，变化 在组织反应以及远离天线的脱落或热损失。 促进剂以低粘度但热敏感的流体形式提供，并固定在适当位置以提供优先吸收或加热的区域。 较短的暴露时间加热远场可能允许脆弱组织如血管的存活，并且多个触角可用于有效治疗不规则或大的肿瘤。

公开(公告)号：WO2017082982A1

公开(公告)日：2017-05-18

申请号：PCT/US2016/047249

申请日：2016-08-17

Applicant: ICECOLDNOW, INC.

Inventor： DUSSAULT, David ,  BECK, Benjamin J. ,  MARSDEN, Douglas A. ,  DONOVAN, Ryan J.

IPC: F28D15/00 ,  G05D23/00 ,  F25B40/00 ,  F25B39/02 ,  F25D17/02 ,  F25D31/00

CPC classification number: F25B1/00 ,  A47J31/44 ,  F25D31/002

Abstract: A coffee appliance includes a powered cooling system integrated with and matched to a hot coffee brewer, configured to cool freshly-brewed coffee by thermal contact to chill a small batch of fresh-brewed coffee in a cooled receiving vessel. The vessel has an evaporator coil to ice the beverage. The cooling system is a robust system, a phase change refrigerant compression-type system employing a positive-displacement compressor, sized in relation to its rate of thermal cooling and the temperature of the beverage and the thermal mass and conductivity of the fluid-contacting assembly, bringing hot coffee to an ice-cold temperature, 2-5 °C, on demand and quickly. The fresh brewed, flash-cooled coffee has undiluted and undegraded flavor. An integrated appliance includes a coffee brewer and cooler in a single device, and a slide switch or valve allows the user to select hot or iced coffee.

Abstract translation: 咖啡器具包括与热咖啡煮泡器集成并与热咖啡煮泡器匹配的动力冷却系统，其配置为通过热接触来冷却新煮泡的咖啡以冷却小批量的在冷却的接收器中的新鲜煮咖啡 船只。 该容器具有蒸发器盘管以将饮料冰冻。 该冷却系统是一个坚固的系统，一种采用容积式压缩机的相变致冷剂压缩式系统，其尺寸与其热冷却速率和饮料的温度以及流体接触组件的热质量和传导率 ，根据需要并迅速将热咖啡带入2-5℃的冰冷温度。 新鲜酿造的闪光咖啡具有未经稀释和未降解的风味。 集成设备包括一台咖啡机和一台冷却器，滑动开关或阀门可让用户选择热咖啡或冰咖啡。

公开(公告)号：WO2017040258A1

公开(公告)日：2017-03-09

申请号：PCT/US2016/048919

申请日：2016-08-26

Applicant: CRAWFORD, Keith D. ,  VASIR, Baldev ,  GARVEY, John

Inventor： CRAWFORD, Keith D. ,  VASIR, Baldev ,  GARVEY, John

IPC: A61K35/12 ,  A61K35/545 ,  C12N5/074 ,  C12N5/0789 ,  G01N33/50

CPC classification number: A61K35/545 ,  C12N5/0668 ,  G01N33/56966

Abstract: Methods, compositions and cells are provided that identify and isolate a population of adult non-embryonic progenitor cells having multilineage potential, physical diameters of about 2 ?m to about 8 ?m in size or about 4 ?m to about 6 ?m, and expressing at least one of the stem cell associated genes among Oct-4, KLF-4, Nanog, Sox-2, Rex-1, GDF-3 or Stella. Methods are also provided that identify and isolate populations, which are subsets or subpopulations of progenitor adult stem cells within the population of the adult stem cells which is a heterogeneous population, the methods including contacting the adult stem cells with a ligand specific for at least one of: CD99, tetraspan, ICAM4, full-length MUC1, and truncated MUC1 receptor, in which a presence of a surface protein on the cells that bind to the ligand identifies the population which is the subset of the differentiated progenitor adult stem cells.

Abstract translation: 提供方法，组合物和细胞，其鉴定和分离具有多线性电位，物理直径约2μm至约8μm或约4μm至约6μm的成体非胚胎祖细胞群体，以及 在Oct-4，KLF-4，Nanog，Sox-2，Rex-1，GDF-3或Stella中表达至少一种干细胞相关基因。 还提供了鉴定和分离群体的种群，其是作为异质群体的成体干细胞群体内的祖细胞成体干细胞的子集或亚群，所述方法包括使成体干细胞与至少一种特异性的配体接触 的CD99，tetraspan，ICAM4，全长MUC1和截短的MUC1受体，其中与配体结合的细胞上的表面蛋白的存在识别作为分化的祖细胞成体干细胞的子集的群体。

公开(公告)号：WO2015057917A1

公开(公告)日：2015-04-23

申请号：PCT/US2014/060818

申请日：2014-10-16

Applicant: BROWN UNIVERSITY

Inventor： SUN, Shouheng ,  PETERSON, Andrew ,  ZHU, Wenlei ,  MICHALSKY, Ronald

IPC: B01J23/52

CPC classification number: C25B11/0473 ,  B01D53/8671 ,  B01D2252/30 ,  B01D2255/106 ,  B01D2255/806 ,  B01D2255/9202 ,  B01D2256/20 ,  B01D2257/504 ,  B01D2258/0283 ,  B01D2259/80 ,  C25B1/00 ,  C25B11/0405 ,  C25B11/0415 ,  C30B29/02 ,  Y02P20/152

Abstract: Selective electrocatalytic reduction of carbon dioxide (CO 2 ) to carbon monoxide (CO) on gold (Au) nanoparticles (NPs) in 0.5 M KHCO 3 at 25 °C. Among monodisperse 4-, 6-, 8-, and 10-nm NPs tested, the 8 nm Au NPs show the maximum Faradaic efficiency (FE), up to 90% at -0.67 V vs. reversible hydrogen electrode. Density functional theory (DFT) calculations suggest that edge sites dominate over corner sites on the Au NP surface facilitating stabilization of the reduction intermediates, such as COOH*, and the formation of CO. This mechanism is further supported by the fact that Au NPs embedded in a matrix of butyl-3methylimidazolium hexafluorophosphate for more efficient COOH* stabilization exhibit even higher reaction activity (3 A/g mass activity) and selectivity (97% FE) at -0.52 V (vs. RHE). Use of monodisperse Au NPs to optimize the available reaction intermediate binding sites thus allows efficient and selective electrocatalytic reduction of CO 2 to CO.

Abstract translation: 在25℃下在0.5M KHCO 3中的金（Au）纳米颗粒（NP）上的二氧化碳（CO 2）到一氧化碳（CO）的选择性电催化还原。 在测试的单分散4-，6-，8-和10-nm NP中，8nm Au NP显示出最大的法拉第效率（FE），在-0.67V时相对于可逆氢电极高达90％。 密度泛函理论（DFT）计算表明，边缘位点主要位于Au NP表面上的拐角位置，有利于还原中间体（如COOH *）的稳定化和CO的形成。这一机理进一步得益于Au纳入嵌入 在用于更有效的COOH *稳定性的丁基-3甲基咪唑六氟磷酸盐的基质中，在-0.52V（vs.RVE）下表现出甚至更高的反应活性（3A / g质量活性）和选择性（97％FE）。 使用单分散金纳米粒子优化可用的反应中间体结合位点，因此可以有效地选择性地将CO 2还原成CO。

公开(公告)号：WO2015017772A1

公开(公告)日：2015-02-05

申请号：PCT/US2014/049395

申请日：2014-08-01

Applicant: PARCELL LABORATORIES, LLC

Inventor： CRAWFORD, Keith D. ,  GARVEY, John ,  WHITE, Colin ,  LAYTON, Pamela

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/074 ,  C12N5/0789

CPC classification number: C12N5/0668 ,  A61K9/0019 ,  A61K35/16 ,  A61K35/28 ,  A61K38/1825 ,  A61K38/1866 ,  A61K38/1875 ,  A61K38/22 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/414 ,  A61L2430/34 ,  C12N5/0607 ,  C12N2521/10 ,  A61K2300/00

Abstract: Grafts, systems, methods and kits are provided for treating a subject having a defect using adult stem cells that are characterized in that the cells do not substantially induce a T cell or natural kill (NK) cell-mediated immune response in the subject. The systems, methods and kits optionally further include exogenous biological factors and energy sources such as an ultrasound device for stimulating healing of the target tissue and tissue defect and/or tissue formation.

Abstract translation: 提供移植物，系统，方法和试剂盒用于使用成体干细胞治疗具有缺陷的受试者，其特征在于细胞在受试者中基本上不诱导T细胞或天然杀伤（NK）细胞介导的免疫应答。 系统，方法和试剂盒任选地还包括外源性生物因子和能量源，例如用于刺激靶组织愈合的超声波装置和组织缺陷和/或组织形成。

公开(公告)号：WO2014031727A1

公开(公告)日：2014-02-27

申请号：PCT/US2013/055955

申请日：2013-08-21

Applicant: BROWN UNIVERSITY

Inventor： PARK, William Keun Chan ,  MILLS, David R. ,  WALSH, Edward G.

IPC: A61K49/04 ,  A61K39/395 ,  A61P35/00

CPC classification number: A61K49/1875 ,  C07K16/2896 ,  G01N33/57492 ,  G01N33/57496 ,  G01N2333/47

Abstract: An MRI contrast material includes tumor-targeting metal-loaded ferritin nanoparticles constructed with genetically modified ferritin coupled to a target-specific agent. Ferritin derived from Archaeoglobus fulgidus (AfFtn-AA) forms hollow nanocages surrounding paramagnetic or superparamagnetic metal core, storing a significantly greater quantity of iron (approximately 7,000 Fe ions per ferritin cage) or other paramagnetic or superparamagnetic metal than natural ferritins, and is conjugated via a short linker with a monoclonal antibody against a cell surface antigen overexpressed by a cancer, to selectively and efficiently attach to tumor cells to enhance MRI contrast. Significant T 2 contrast with diminished T 1 effect was observed owing to the heterogeneous nanoconjugate distribution when bound to cells. In a treatment method, after imaging, an external stimulus heats the cell-bound agent to release the metal and selectively destroy the targeted cells. The enhanced imaging and release of toxic metal ions provides simultaneous early detection and treatment.

Abstract translation: MRI造影材料包括用与靶特异性试剂偶联的遗传修饰的铁蛋白构建的肿瘤靶向金属负载的铁蛋白纳米颗粒。 衍生自古细菌球菌（AfFtn-AA）的铁蛋白形成围绕顺磁性或超顺磁性金属核的中空纳米笼，与天然铁蛋白相比，存储显着更大量的铁（每个铁蛋白笼约7,000个Fe离子）或其他顺磁性或超顺磁性金属， 具有针对由癌细胞过表达的细胞表面抗原的单克隆抗体的短接头，选择性且有效地附着于肿瘤细胞以增强MRI对比度。 当与细胞结合时，由于异质纳米缀合物分布，观察到T1效应降低的显着T2对比。 在处理方法中，在成像之后，外部刺激加热细胞结合剂以释放金属并选择性地破坏靶细胞。 有毒金属离子的增强成像和释放提供同时的早期检测和处理。

公开(公告)号：WO2012162660A2

公开(公告)日：2012-11-29

申请号：PCT/US2012/039699

申请日：2012-05-25

Applicant: BROWN UNIVERSITY ,  KELSEY, Karl ,  HOUSEMAN, Eugene, Andres ,  WIENCKE, John ,  ACCOMANDO, William, P., Jr. ,  MARSIT, Carmen

Inventor： KELSEY, Karl ,  HOUSEMAN, Eugene, Andres ,  WIENCKE, John ,  ACCOMANDO, William, P., Jr. ,  MARSIT, Carmen

IPC: C12Q1/68 ,  C12N15/11 ,  G06F17/10

CPC classification number: C12Q1/6883 ,  C12Q2600/154 ,  G06F19/18 ,  G06F19/24

Abstract: Methods using DNA Methylation arrays are provided for identifying a cell or mixture of cells and for quantification of alterations in distribution of cells in blood or in tissues, and for diagnosing, prognosing and treating disease conditions, particularly cancer. The methods use fresh and archival samples.

Abstract translation: 提供使用DNA的方法甲基化阵列用于鉴定细胞或细胞混合物，并用于定量血液或组织中细胞分布的变化，以及用于诊断，预后和治疗疾病状况，特别是癌症。 该方法使用新鲜和档案样品。

公开(公告)号：WO2012019134A2

公开(公告)日：2012-02-09

申请号：PCT/US2011/046810

申请日：2011-08-05

Applicant: BROWN UNIVERSITY ,  HUANG, Yongsong ,  APONTE, Jose C. ,  TAROZO, Rafael ,  DILLON, James

Inventor： HUANG, Yongsong ,  APONTE, Jose C. ,  TAROZO, Rafael ,  DILLON, James

IPC: B01J20/282 ,  B01J20/291 ,  G01N30/02 ,  C07C7/12 ,  B01D15/08

CPC classification number: B01J20/0262 ,  B01J20/289 ,  B01J20/291 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3236 ,  B01J2220/62

Abstract: Methods, compositions, devices and kits having a novel chromatographic material are provided herein for separating and identifying organic molecules and compounds, for example molecules and compounds containing electron rich functional groups such as carbon-carbon double bonds. The methods, compositions, and kits include a metal-thiolate chromatographic medium (MTCM) with a sulfur-containing functional group or a metal-selenolate chromatographic medium (MSCM) comprising a selenium-containing functional group covalently attached to a support medium, such that the sulfur-containing functional group or selenium-containing functional group is bound to at least one metal atom. The MTCM and/or MSCM has affinity and specificity to compounds having one or more carbon-carbon double bonds, and performs a highly efficient and rapid separation of samples yielding non-overlapping peaks of purified materials compared to traditional media.

Abstract translation: 本文提供了具有新型色谱材料的方法，组合物，装置和试剂盒，用于分离和鉴定有机分子和化合物，例如含有富电子官能团如碳 - 碳双键的分子和化合物。 所述方法，组合物和试剂盒包括具有含硫官能团的金属硫醇盐色谱介质（MTCM）或包含共价连接至载体介质的含硒官能团的金属硒酸盐色谱介质（MSCM），使得 含硫官能团或含硒官能团与至少一个金属原子结合。 MTCM和/或MSCM对具有一个或多个碳 - 碳双键的化合物具有亲和力和特异性，并且与传统培养基相比，对样品进行高效且快速的分离，产生纯化材料的非重叠峰。