公开(公告)号：WO2012141471A3

公开(公告)日：2012-12-06

申请号：PCT/KR2012002696

申请日：2012-04-10

Applicant: COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND ,  HWANG DONG-YOUN ,  LEE KANG-IN

Inventor： HWANG DONG-YOUN ,  LEE KANG-IN

IPC: C12N5/0735 ,  C12N5/02

CPC classification number: C12N5/0696 ,  C12N2501/727

Abstract: The present invention relates to a method for preparing induced pluripotent stem cells (iPS cells), comprising: (a) culturing human-derived somatic cells, into which genes encoding differentiation-inducing factors are introduced, in a medium containing one or more dedifferentiation enhancers selected from a group consisting of a protein kinase C inhibitor, a histone deacetylase inhibitor, and a bone morphogenetic protein (BMP) pathway blocker; and (b) separating embryonic stem cell-like colonies from the culture obtained in step (a). The method of the present invention may enable the preparation of induced pluripotent stem cells with high efficiency, as well as the preparation of induced pluripotent stem cells under xenopathogen-free and feeder cell-free conditions.

Abstract translation: 本发明涉及制备诱导性多能干细胞（iPS细胞）的方法，其包括：（a）在含有一种或多种去分化促进剂的培养基中培养来源于人的体细胞，在所述体细胞中导入编码分化诱导因子的基因 选自由蛋白激酶C抑制剂，组蛋白脱乙酰酶抑制剂和骨形态发生蛋白（BMP）通路阻断剂组成的组; 和（b）从步骤（a）中获得的培养物中分离胚胎干细胞样集落。 本发明的方法可以使得能够高效地制备诱导性多能干细胞，以及在无异种原性和无饲养细胞条件下制备诱导性多能干细胞。

公开(公告)号：WO2009014340A3

公开(公告)日：2009-03-19

申请号：PCT/KR2008004159

申请日：2008-07-16

Applicant: COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND ,  LEE SOO-HONG ,  AHN SEONG-EUN ,  LEE DAE-HEE

Inventor： LEE SOO-HONG ,  AHN SEONG-EUN ,  LEE DAE-HEE

IPC: C12N5/0735 ,  C12N5/077

CPC classification number: C12N5/0606 ,  C12N5/0654 ,  C12N2502/03 ,  C12N2502/1394 ,  C12N2506/02 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/90

Abstract: The present invention provides a process for differentiating embryonic stem cells by co-culturing embryonic stem cells or an embryoid body derived from embryonic stem cells in a differentiation culture medium comprising feeder cells, wherein the culture medium comprises a porous membrane having the feeder cells attached to a bottom of the porous membrane, and the embryonic stem cells or embryoid body are co-cultured on the porous membrane; and a process for recovering cells differentiated from embryonic stem cells using the same.

Abstract translation: 本发明提供了一种通过在包含饲养细胞的分化培养基中共培养胚胎干细胞或源自胚胎干细胞的胚状体来分化胚胎干细胞的方法，其中所述培养基包含多孔膜，所述多孔膜的饲养细胞附着于 多孔膜的底部和胚胎干细胞或拟胚体在多孔膜上共培养; 以及使用该方法回收从胚胎干细胞分化的细胞的方法。

公开(公告)号：WO2011099736A2

公开(公告)日：2011-08-18

申请号：PCT/KR2011000799

申请日：2011-02-08

Applicant: COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND ,  LEE SOO-HONG ,  KANG SUN-WOONG ,  KIM JIN-SU ,  CHA BYUNG-HYUN

Inventor： LEE SOO-HONG ,  KANG SUN-WOONG ,  KIM JIN-SU ,  CHA BYUNG-HYUN

IPC: A61L27/14 ,  A61L27/38 ,  A61L27/56 ,  C12N11/02

CPC classification number: A61L27/56 ,  A61L27/58

Abstract: The present invention relates to a method for manufacturing a porous scaffold for tissue-reconstruction, the method comprising the steps of performing a heat treatment on biodegradable polymers and stacking the heat-treated biodegradable polymers obtained thereby, wherein the heat treatment is performed on modified biodegradable polymers which have been obtained by substituting a hydrogen of a terminal hydroxy group of the biodegradable polymer with a C1~C4 alkylcarbonyl group.

Abstract translation: 本发明涉及一种用于组织重建的多孔支架的制造方法，所述方法包括以下步骤：对可生物降解的聚合物进行热处理，并堆叠由此得到的经热处理的可生物降解的聚合物，其中对经改性的可生物降解 通过用C 1 -C 4烷基羰基取代生物可降解聚合物的末端羟基的氢而获得的聚合物。

公开(公告)号：WO2010123205A2

公开(公告)日：2010-10-28

申请号：PCT/KR2010001792

申请日：2010-03-24

Applicant: COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND ,  CHUNG KWANG HOE ,  LEE KWANG-HYOUNG ,  CHOI HAENG-SUK ,  KO JUNG-JAE

Inventor： CHUNG KWANG HOE ,  LEE KWANG-HYOUNG ,  CHOI HAENG-SUK ,  KO JUNG-JAE

IPC: B01F11/00 ,  B01F15/06

CPC classification number: B01F11/0008 ,  B01F11/0014 ,  B01F15/065 ,  B01F2015/062 ,  B01L7/00 ,  B01L9/06 ,  B01L9/52 ,  B01L2300/1827

Abstract: A constant temperature agitating device for agitating substances received in a container comprises a base, a container receiving body part and a shaking motion part. The container receiving body part is positioned at an upper part of the base, and includes upper and lower body parts for covering the upper and lower parts of the container so as to receive the container, wherein the inner surfaces of the upper and lower body parts which contact with the container form a container receiving part corresponding to the outer surface of the container. The shaking motion part shakes the container receiving body part against the base. The container receives heat via the inner surface contacting with the container receiving part.

Abstract translation: 用于搅拌容纳在容器中的物质的恒温搅拌装置包括基座，容器容纳体部和摇动部分。 容器容纳体部分位于基部的上部，并且包括用于覆盖容器的上部和下部以容纳容器的上部和下部主体部分，其中上部和下部主体部分的内表面 其与容器接触形成与容器的外表面相对应的容器接收部分。 摇动运动部件将容器接收体部分摇动到底座上。 容器通过与容器接收部分接触的内表面接收热量。

公开(公告)号：WO2011081328A2

公开(公告)日：2011-07-07

申请号：PCT/KR2010008969

申请日：2010-12-15

Applicant: COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND ,  LEE SOO-HONG ,  KANG SUN-WOONG ,  KIM JIN-SU ,  HAN IN-BO ,  SHIN DONG-AH

Inventor： LEE SOO-HONG ,  KANG SUN-WOONG ,  KIM JIN-SU ,  HAN IN-BO ,  SHIN DONG-AH

IPC: A61L27/44 ,  A61F2/02 ,  A61L27/34 ,  A61L27/54 ,  A61L27/56

CPC classification number: A61L27/54 ,  A61L27/34 ,  A61L27/56 ,  A61L2300/64 ,  C08L89/00 ,  C08L5/08

Abstract: The present invention provides a construct for tissue reconstruction, comprising a coating layer formed by coating a surface of a frame of a polymer support having voids, with hyaluronic acids or salts thereof and fibrinogens, wherein (i) the coating layer comprises: cells; cell growth factors; or cells and cell growth factors, (ii) the coating layer is cross-linked by thrombin, and (iii) the construct has voids of 10 µm to 1,000 µm. In addition, the present invention provides a kit for producing a construct for tissue reconstruction, which is cross-linked by thrombin and has voids of 10 µm to 1,000 µm.

Abstract translation: 本发明提供了用于组织重建的构建体，其包括通过用透明质酸或其盐和纤维蛋白原涂覆具有空隙的聚合物载体的框架的表面而形成的涂层，其中（i）所述涂层包括：细胞; 细胞生长因子; 或细胞和细胞生长因子，（ii）涂层通过凝血酶交联，和（iii）构建体具有10μm至1,000μm的空隙。 此外，本发明提供了一种用于组织重建用构件的制备工具，其通过凝血酶交联并具有10μm〜1000μm的空隙。

公开(公告)号：WO2011159050A3

公开(公告)日：2012-05-03

申请号：PCT/KR2011004118

申请日：2011-06-07

Applicant: COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND ,  MOON JI-SOOK

Inventor： MOON JI-SOOK

IPC: C12N5/0793 ,  C12N5/02

CPC classification number: C12N5/0618 ,  C12N5/0619 ,  C12N2500/02 ,  C12N2501/01 ,  C12N2501/119 ,  C12N2501/41 ,  C12N2501/815

Abstract: The present invention provides a method for differentiating human neural progenitor cells into dopaminergic neurons, comprising the step of culturing human neural progenitor cells in a medium containing fusaric acid. In addition, the present invention provides a medium for differentiation of human neural progenitor cells into dopaminergic neurons.

Abstract translation: 本发明提供了将人神经祖细胞分化为多巴胺能神经元的方法，其包括在含有镰孢酸的培养基中培养人神经祖细胞的步骤。 此外，本发明提供了将人神经祖细胞分化为多巴胺能神经元的培养基。

公开(公告)号：WO2009125936A3

公开(公告)日：2009-12-30

申请号：PCT/KR2009001614

申请日：2009-03-30

Applicant: COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND ,  BAEK KWANG-HYUN ,  KIM MYUNG-SUN ,  KIM YONG-SOO

Inventor： BAEK KWANG-HYUN ,  KIM MYUNG-SUN ,  KIM YONG-SOO

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158

Abstract: The present invention relates to an analysis method including a step of measuring the amount of gene expression for coding one or more proteins selected from a group consisting of proteins of sequence numbers 1 to 4 in a patient specimen to provide information required for diagnosis of polycystic ovary syndrome. The present invention further relates to a kit for the diagnosis of polycystic ovary syndrome including a reagent for measuring the amount of gene expression for coding one or more proteins selected from a group consisting of proteins of sequence numbers 1 to 4.

Abstract translation: 本发明涉及一种分析方法，其包括测量用于编码患者样品中选自由序列号1至4的蛋白质组成的组中的一种或多种蛋白质的基因表达量，以提供诊断多囊卵巢所需的信息的步骤 综合征。 本发明还涉及用于诊断多囊性卵巢综合征的试剂盒，其包括用于测量编码选自由序列号1至4的蛋白质组成的组中的一种或多种蛋白质的基因表达量的试剂。